• The Korean Journal of Mycology
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• v.11 no.2
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• pp.85-89
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• 1983

The aims of this study were to investigate various components and their physiological activities of Sarcodon aspratus (Berk.) S. Ito which grows wildly in Korea, belonging to the family Thelephoraceae. The analysis of the powered carpophore of this fungus by TLC and an amino acid autoanalyzer revealed that it contained twenty-one free amino acids and that twenty­two total amino acids were identified in its acid hydrolysate. These amino acids were also quantified.

• Journal of Ecology and Environment
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• v.29 no.2
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• pp.143-149
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• 2006

This study was conducted to identify native ectomycorrhizal (ECM) fungi colonizing Pinus densiflora for revegetation of abandoned coal mines in Korea. Seedlings of P. densiflora growing on coal mining spoils of a study site in Samcheok were collected. ECM roots were observed under stereomicroscope and their DNA were extracted from each root tip for a seedling for molecular identification. A PCR primer pair specific to fungi, ITS1F and ITS4, was used to amplify fungal DNA. Restriction enzymes, Alul and Hinfl were used for restriction fragment length polymorphism (RFLP). Combined with RFLP profiles and sequence analysis, total twenty one taxa were identified from the ECM root tips. Basidiomycetous fungi including Thelephoraceae, Pezizales, Laccaria, Pisolithus and Ascomycetous fungi including ericoid mycorrhizal fungi were identified from this study. Results showed that the most frequently found in the study sites was a species in Thelephoraceae. A possible use of ECM fungi identified in this study for the revegetation of abandoned coal mines with P. densiflora was discussed.

• The Korean Journal of Mycology
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• v.14 no.1
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• pp.25-30
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• 1986

The purposes of this study were to investigate enzyme components and its physiological activities of Sarcodon aspratus (Berk.) S. Ito which grows wildly in Korea, belonging to the family Thelephoraceae. The carpophores of the fungus was extracted with cooling distilled water and salted out by ammonium sulfate. The precipitate was purified by dialysing through visking tube against distilled water and then dissolved with pH 7.8 ammonia aqua, and the extract was filterated. The fraction of filtrate was obtained as light brown powder after lyophilization and determined proteolytic activity. Protease activity of Sarcodon aspratus (Berk.) S. Ito was about two-third of that of pepsin on casein by cup method. The proteolytic potency of this enzyme was found to be 500 unit/mg. This proved the efficacy of the mushroom when it was used as a folk medicine for treating indigestion of beef.

• The Korean Journal of Mycology
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• v.11 no.4
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• pp.159-162
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• 1983

The dried carpophores of Sarcodon aspratus were examined for mineral elements and a protein-polysacccharide. Calcium, iron, zinc, magnesium, manganese, copper, lead, cadmium and mercury were detected in that order of contents by atomic absorption spectrometry and automatic mercury analysis. The protein-polysaccharide fraction extracted from the carpophore was found to contain 31.5% protein and 52.8% polysaccharide. The fraction showed no antitumor activity against sarcoma 180 implanted in mice.

• Korean Journal of Pharmacognosy
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• v.33 no.3 s.130
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• pp.224-229
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• 2002

Sarcodon aspratus (Thelephoraceae), a native mushroom distributed in Korea and Japan has been widely used as a traditional food and folk medicines. Two proteoglycan polysaccharides, named SAP-A and SAP-B were isolated from the fruitbody of S. aspratus. Their molecular weight were determined as 6184.9 D, and 25749.2 D, respectively, by maldi-tof ms. The composition of sugar and amino acid of these compounds was also determined. The SAP-A showed potent antitumor activity. The $ID_{50}$ value of SAP-A was 8.2 mg/kg/day for ICR mice transplanted with solid sarcoma-180, and the elongation of lifespan effect was 167.4% for ICR mice transplanted with ascitic sarcoma-180. Moreover, the elongation life-span effect of SAP-A increased dose-dependently.

• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.591-596
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• 1996

In the course of screening lipid peroxidation inhibitor from basidiomycetes, a mushroom, which was collected at O-Dae mountain in Kangweon- Do, was found to have active compound. The mushroom was identified as Polyzellus multiplex, which belongs to Aphylloporalles Thelephoraceae, on the basis of macroscopic and microscopic characteristics of the fruiting body. The methanol extract of fruiting body was extracted with benzene and ethylacetate, sequentially. By using various kinds of chromatographies, PM1, and PM2 and PM3, were purified from the ethylacetate extract and the benzene extract, respectively. Color reaction and analyses of IR, UV, and NMR spectra indicated that PM1 was a derivative of thelephoric acid, and PM2 and PM3 were linoleic acid and oleic acid, respectively. IC$_{50}$ of PM1 for inhibition of lipid peroxidation was 1.96 ppm and LD$_{50}$ was 500 mg/kg.

• The Korean Journal of Mycology
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• v.22 no.1
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• pp.62-99
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• 1994

The fungal flora of Korean wood-rotting fungi were studied for two years from March of 1990 to February of 1992. Fresh fungi were collected from national parks, local areas, and islands throughout the country. Fleshy fungi of the Aphyllophorales were identified through specimen examination and literature studies. Total 217 species and 1 variety were counted, among which Aleurodiscus cerussatus, Botryobasidium obtusisporum, Ceraceomyces cystidiatus, Erythricium hypnophilum, Grandinia crustosa, Grandinia spathulata, Hyphoderma praetermissum, Hyphoderma roseocremeum, Hypochnicium bombycinum, Hypochnicium detriticum, Hypochnicium lundellii, Laeticorticium roseum, Mycoacia fuscoatra, Peniophora incarnata, Phanerochaete avellanea, Phanerochaete filamentosa, Phanerochaete martelliana, Phlebia lilascens, and Trechispora vaga under the Corticiaceae, Chondrostereum purpureum and Cystostereum subabruptum under the Stereaceae, Tomentella pilosa under the Thelephoraceae, Asterostroma laxum, Hymenochaete cruenta, Hymenochaete fuliginosa, Hymenochaete tabacina, Inonotus radiatus, and Phellinus pomaceus under the Hymenochaetaceae, Antrodia crassa, Antrodia serialis, Ceriporia reticulata, Oligoporus balsameus, Oligoporus guttulatus, Oxyporus cuneatus, Rigidoporus microporus, and Trichaptum laricinum under the Polyporaceae, total 36 species were confirmed as unrecorded species to Korea and are registered here with Korean names and English descriptions.