• Journal of Korean Society of Forest Science
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• v.81 no.2
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• pp.156-163
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• 1992

Pinus koraiensis (Pk), P. rigida (Pr) and P. rigida ${\times}$ P. taeda (Pr. t) seedlings in a bare-rooted nursery were artificially inoculated with Pisolithus tinctorius (Pt) and Thelephora terrestris (Tt) to test long term effects of ectomycorrhizal inoculation on host growth. Mycelial inocula of Pt and Tt were mass-cultured in vermiculite-peatmoss mixture and introduced into fumigated nursery soil before seed sowing. Bare-rooted, inoculated seedlings at one to four years of age were outplanted to the field with $P_2O_5$ content of 25 ppm in soil. At the time of outplanting, Pk seedlings(4 years old), Pr seedlings(2 years old), and Pr.t seedlings(1 year old) all infected by Pt were significantly taller by 28%. 26%, and 77%, respectively, than controlled seedlings infected by natural population of mycorrhizal fungi in the non-fumigated plot. Ten years after inoculation or six to nine years after outplanting, Pk seedlings inoculated with Pt were significantly taller by 9% Pr.t seedlings significantly taller by 18%, and Pr slightly Caller by 2%(not significant) than controlled seedlings, suggesting that the stimulatory effect of Pt on host growth gradually declined or became minimal after outplanting. Tt failed to stimulate host growth either in the nursery or in the field, and the survival rate of outplanted seedlings was not different among fungal treatments. Considerable loss of the infected root system during lifting the seedlings for outplanting would be the primary cause of the reduced effect of Pt in the field. Pt infected more than 90% of the fine roots in the fumigated nursery during the first growing season, but Pt assumed to fail to compete successfully with natural population of ectomycorrhizal fungi in the field. It is necessary to select other mycorrhizal fungi which adapt well in both nursery and field.

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.22-29
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• 1982

Two ectomycorrhizal fungi, Pisolithus tinctorius and Thelephora terrestris, were introduced form U.S.A. and inoculated to five pine species in Korea to evaluate the reported growth stimulation of host plants after inoculation. These fungi were grown as mycelial inoculum in large quantity and ioculated to the fumigated nursery soil just before seed sowing. At the end of the first growing season. Pisolithus stimulated the height growth of Pinus densiflora. P. thunbergii. P. rigida, and P. rigida x teada by55, 36, 69, and 37%, respectively, compared with control seedlings with no fumigation and no inoculation. When the growth stimulation was expressed with dry weight, Pisolithus increased dry weight of P. densiflora and P. rigida x taeda by 143% and 128%, respectively, over control seedling. Thelephora failed to stimulate growth of inoculated plants. Pinus koraiensis did not respond to the inoculation during the first growing season. It is concluded that artificial inoculation of nursery pine trees with selected mycorrhizal fungi should be seriously considered to improve the quality of planting stocks and to stimulate early plant growth. The potential for use of Pisolithus in reforestation on adverse sited is also discussed.

• Journal of Korean Society of Forest Science
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• v.70 no.1
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• pp.45-54
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• 1985

The experiment was conducted to test the effects of ectomycorrhizal inoculation and soil moisture regime on growth and nutrient uptake of Pinus rigida seedlings. Two-year-old seedlings inoculated with either Pisolithus tinctorius or Thelephora terrestris were used for this experiment. There were two moisture treatments and four phosphate treatment levels with three replications. Height and root collar diameter growth and dry weight increase were measured and nitrogen, phosphorous, and potassium in the tissue were analyzed three months after the initiation of phosphate and moisture regime treatment. Results are as follows; 1) Height growth of seedlings was not affected in all treatments. (mycorrhizae, fertility, moisture). 2) High moisture regime increased root collar diameter growth 2.7 times as large as low moisture regime. 3) In low moisture regime, net production increased 26% in seedlings inoculated with Pisolithus tinctorius and increased 72% control seedlings. 4) In high moisture regime, net production of inoculated seedlings with Pisolithus tinctorius increased four times as much as that of control seedlings but increased $P_2O_5$ in fertility level did not affect net production. 5) In uptake of nitrogen, phosphate, and potassium, Pisolithus tinctorius - inoculated seedlings increased uptake by 1.9, 1.8, and 1.5 times, respectively, as much as control seedlings. 6) Increase in $P_2O_5$ fertility level enhanced the uptake of $P_2O_5$ in Pisolithus tinctorius - inoculated seedlings, but not affected the uptake of $P_2O_5$ in control seedlings. 7) Increase in $P_2O_5$ fertility levels enhanced the uptake of $K_2O$ in Pisolirhus rinctorius - inoculated seedlings.