• 전기저널
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• 4호통권64호
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• pp.17-21
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• 1982

• 한국식품조리과학회지
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• 제17권2호
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• pp.149-155
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• 2001

Namul cooked with the standard recipe was examined by research of microbiological test for three months and sensory evaluation of frozen namul after reheating. When the namul was freezed storage, in the microbiological test namul began to change on the 40th day, but there was no problem about stability of storage until 3 months. The overall qualities of taste, flavor, color and texture were examined by sensory evaluation of frozen namul after reheating. The pH was seemed to change slowly, its color was changed on the 20th day from the beginning of storage. Radish root represented substantial difference in texture and overall quality on the 20th day. The off-flavor of immature pumpkin stated on the 25th day. Later 10days nettle tree mushroom began to be changed in its taste, texture, overall quality and appearance, then on the 25th day it was seemed to have low preference. But oak mushroom kept its quality good for 25 days. Oyster mushroom was changed in color, appearance and overall quality on the 20th day. Bracken had low preference in taste, texture and moisture on the 25th day. The color of spinach was changed on the 15th day, and its taste on the 20th day. Soybean sprout was changed in taste, texture and overall quality on the 15th day, and overall quality marked low preference on the 25th day Root of bell flower was changed on the 25th day(p<0.05).

• 한국대기환경학회지
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• 제27권2호
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• pp.168-180
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• 2011

The variation of the physicochemical properties of atmospheric aerosols in coexistence of the heavy Asian Dust and Haze observed from $15^{th}$ to $17^{th}$ March 2009 in Seoul was scrutinized through the mass and ion concentration observations and synoptic weather analysis. Although the ratio of PM1.0/PM10 was constant at 0.3 (which is typical during Asian Dust period in Korea) during the measurement period, both PM10 and PM1.0 mass concentrations were 3~6 times and 2~4 times higher than that of clear days, respectively. Water-soluble ion components accounted for 30~50% of PM10 and 50~70% of PM1.0 mass concentration. One of the secondary pollutants, $NO_3^-$ was found to be associated with $Ca^{2+}$ and $Na^+$ in coarse mode indicating that the aerosol derived from natural source was affected by anthropogenic pollutants. While the acidity of the aerosols increased in fine mode when the stagnation of weather patterns was the strongest (March $16^{th}$), the alkalinity increased in coarse mode when new air masses arrived with a southwestern wind after ending a period of stagnation (March $17^{th}$). In the selected case, SOR (Sulfur Oxidation Ratio, $nSO_4^{2-}/[nSO_4^{2-}+nSO_2]$) and NOR (Nitrogen Oxidation Ratio, $nNO_3^-/[nNO_3^-+nNO_2]$) values of ion components were higher than the general values during Asian Dust period. These results imply that dust aerosols could be mixed with pollutants transported from China even in heavy Asian Dust cases in Korea.

• 보존과학회지
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• 제17권
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• pp.57-64
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• 2005

청평사 강선루 회전문 부근에서 출토된 조선시대 $16\~17$세기 철물과 철촉에 대한 금속조직학적으로 조사하기 위하여 유물에서 부식되지 않은 시편을 채취하여 마운트와 연마 및 부식을 시켜 금속조직을 관찰하고 비금속개재물부분은 SEM으로 관찰한 뒤 EDS로 분석하였다. 금속학적 조직조사와 SEM-EDS분석을 통하여 철물과 철촉은 고체저온환원법에 의한 순철에 가까운 괴련철 혹은 해면철을 이용하여 철을 생산하고, 단야로 같은 곳에서 침탄을 하여 탄소량을 높인 것으로 밝혀졌다. 또한 철물은 탄소량을 어느 정도 높인 다음 담금질과 열처리과정을 반복하여 철기의 강도를 인위적으로 많이 높이는 작업을 한 것으로 밝혀졌다. 이번 조사연구를 통하여 조선시대에도 괴련철을 소재로 하여 철을 생산하였다는 것을 알 수 있어 의미 있는 결과를 얻었다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권2호
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• pp.161-166
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• 2000

This study was designed to determine the effects of the insulin-like growth factor (IGF-1) and estradiol $17-{\beta}$ on the in vitro proliferation of stromal vascular cell from Hanwoo omental, subcutaneous, intermuscular and intramuscular adipose tissues. Cells were cultured in M199+20% newborn calf serum and the proliferation of cells was measured by direct microscopic cell counting and change of genomic DNA amount. Cell numbers increased slightly over the first 72 hour of culture and then increased greatly, regardless of adipose tissue depots. In IGF-1 treatment, the number of omental preadipocytes maintained highest level from the beginning to the 20th day of culture. However, in estradiol-$17{\beta}$ treatment, those tended to be lower than the control from the beginning of culture and significantly lower at the 24th day. When IGF-1 was added to subcutaneous preadipocytes, the numbers of cells were higher from 11th day than those from other treatments, although there was no statistical significance. For intermuscular preadipocytes treated with IGF-1, its numbers were significantly (p<0.05) higher at 11th day, and in the other days it showed a similar tendency to those of the subcutaneous tissue. In this experiment, preadipocytes were taken from 24 month old fully matured steers and the highest proliferation rate was shown in intramuscular tissue followed by those of subcutaneous preadipocytes. Addition of $5{\mu}M$ estradiol-$17{\beta}$ to the growth medium failed to promote the replication of Hanwoo preadipocytes, as indicated by direct cell counts and total genomic DNA content. As the culture period proceeded, the amounts of DNA were increased, but the patterns of increment were not consistent with the results of cell numbers.

• 대한한방부인과학회지
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• 제23권1호
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• pp.30-41
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• 2010

Purpose: This study was to observe the effect of "herbal decoction for sitz bath" on dermoepidermal recovery to wound tissue in rat's skin. Methods: The samples were assigned to 3 groups: control group : without any treatment, positive control group : potarose 10% solution, experiment group : herbal decoction for sitz bath. We made the open wound of $2{\times}2cm^2$ size that cut deep into the dermis. Treating the open wound for 17 days, we observed the size of the wound diminishing. On 17th days, the cell viability was measured by MTT assay. The effect anti-inflammatory and dermoepidermal recovery were examined by H&E staining, immunohystochemical staining for MIP-2, FGF. Results: The experiment group showed more recovery from the open wound comparing the control group and the positive control group on 10th days after wounding. But there was not remarkable difference between the experiment and positive control group after 17th days post-wounding. The number of MIP-2 positive reacted cell were significantly decreased and that of FGF positive reacted cell were significantly increased than positive control group at 17th days. Conclusion: According to these results, we finally concluded that "herbal decoction for sitz bath" could be effective in recovery to wound tissue.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제13권1호
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• pp.1-8
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• 1991

체중 100g 내외의 Sprague Dawley계 정상백서 암, 수 각 50마리씩을 실험동물로 하여 분말형태의 순수 DMBA를 소구(pellet)로 압착제작하여 우측 악하선에 매식후 즉시, 1,3,5,7,9,11,13,15,17주째 10마리씩 희생시켜 좌, 우측악하선을 적출, 무게를 측정하고, 육안적, 조직학적, 생화학적으로 실험기간에 따라 비교분석하여 다음과 같은 결론을 얻었다. 1. 매식후 11 주째부터 우측 경부 악하선부위의 종창을 관찰할 수 있었으며, 결절덩어리를 촉진할 수 있었다. 2. 우측악하선의 무게의 크기는 실험기간의 경과에 따라 괄목할만한 증가를 보였다(매식후 17주째는 좌측에 비해 약 5.5배의 무게를 나타냈다). 3. 매식후 7주와 11주째부터 조직학적으로 상피이형성과 침윤성 유표피암의 소견을 각각 관찰할 수 있었다. 4. 우측 악하선에서의 유표피암의 발생율은 매식후 17주째 76%이었다. 5. DMBA 는 현저히 억제된 $Na^+,K^+$-ATPase 활성도를 야기하며 실험기간의 경과의 따라 활성도 비율도 현저히 저하시킨다.

• IMMUNE NETWORK
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• 제20권6호
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• pp.50.1-50.11
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• 2020

Osteoporosis is prevalent in elderly women and it may cause dental implant failure. In particular, estrogen deficiency in postmenopausal women leads to higher rates of osteoporosis prevalence. Immune cell-mediated effects involving the development of osteoporosis have been studied previously; however, the role of IL-10-producing regulatory B (B10) cells in osteoporosis is largely unclear. Here, we examined the role of B10 cells in osteoporosis. C57BL/6 mice were subjected to ovariectomy (OVX). Fifteen weeks after OVX surgery, the first molar of the right maxillary was extracted, and twenty-four weeks after OVX surgery, serous progression of osteoporosis was observed in the alveolar bone. Moreover, the proportion of CD19⁺CD5⁺CD1d^high regulatory B cells, B10, and CD4⁺CD25⁺FoxP3⁺ regulatory T cells from the spleen of OVX mice decreased during the progression of osteoporosis, compared to controls. In contrast to regulatory cells, IL-17-producing Th (Th17) cell levels were increased in OVX mice. Adoptive transfer of B10 cells to OVX mice led to a decrease in Th17 cell abundance and inhibited the development of osteoporosis in the alveolar bone from OVX mice. Thus, our results suggest that B10 cells may help suppress osteoporosis development.

• 동의생리병리학회지
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• 제23권5호
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• pp.1106-1115
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• 2009

Mori Ramulus has multiple applications in Korean traditional medicine prescription because it has antioxidant and anti-inflammatory effects by reducing macrophage activities. Yet, no studies on the anti-arthritic activity of EMR (extract of Mori Ramulus) have been reported in vitro and in vivo. Rheumatoid arthritis (RA) is a systemic autoimmune disease with chronic inflammation characterized by hyperplasia of synovial cells in affected joints, which ultimately leads to the destruction of cartilage and bone. Because collagen-induced arthritis (CIA) is similar to RA in pathological symptoms and immune reactions, there have been several reports concerning RA using CIA mouse model. Here, we investigated the effects of Mori Ramulus on RA using CIA mice. The importance of CD4+ Th1 cells in RA progress was previously indicated and studies further showed that Th17 cells play a prime role in severity of disease. Accordingly, the present study was focused on CIA associated with CD4+ Th1 cells and Th1 7 cells. DBA/1OlaHsd mice were immunized with bovine type II collagen (CII). After a second collagen immunization, mice were treated with EMR once a day for 4 weeks. The severity of arthritis within the paw joints was evaluated by histological assessment of cartilage destruction and pannus formation. Immune cells in peripheral blood mononuclear cells (PBMC), draining lymph node (DLN) and paw joints, cytokine production and gene expression were assessed from CIA mouse using ELISA, FACS and real-time PCR analysis. Administration of EMR significantly suppressed the progression of CIA and inhibited the production of TNF-$\alpha$, IL-6 and IL-17 in the serum. The erosion of cartilage was dramatically reduced in mouse knees after treatment with EMR. In conclusion, our results demonstrate that EMR significantly suppressed the progression of CIA and that this action was mediated by the decreased production of TNF-$\alpha$, IL-6, IL-17 and collagen II-specific antibody in the serum. EMR suppressed Th17 cells and reduced level of IL-6 via B cell suppression, and thus, the levels of autoantibodies produced from B cells were decreased. Furthermore, EMR suppressed NKT cells which directly stimulate B cells and develop imbalance of Th1/Th2 cell. Oral administration of EMR (100 mg/kg or 200 mg/kg) significantly suppressed the progression of CIA, which is comparable to that of methotrexate (MTX, 0.3 mg/kg) used as a positive control. We are currently studying the mechanism underlying the therapeutic role for EMR in CIA mice.

• 건축역사연구
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• 제16권6호
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• pp.67-86
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• 2007

Choseon Dynasty, from many aspects, saw the institutional establishment of its royal palaces in the 17th and 18th century, with 'donggwol (east palace)' as the most representative form in the era. In that period, palaces were managed in the best way that fits the royal etiquette and order to maintain the Confucian framework of the times. While the royal palace was the place for the king to conduct state affairs, it was also a compound for the royal family to lead a life in. Since the royal family was also based on the Confucian system, women in the royal palace seldom revealed their existence to outside world. Yet daebi,(a Queen Mother) who was often called 'dongjo,' enjoyed the highest level of honor not only as a member of the royal family but in the hierarchical order of the dynasty. As they often engaged themselves in political affairs, daebi raised their reputation through rites and rituals. So, in the 16th century, they largely used Changgyeong-gung palace in the eastern part of the royal compound since they sometimes had to go out of the royal residence. While it was called 'dongjo' because it was seated in the eastern part, it was also used as a word symbolizing daebi. And, therefore, it has become a general principle of royal palaces to build the palace for daebi in the eastern wing of the compound. However, the residence for daebi was not always built in the eastern part in the 17th and 18th century and, instead, edifices for daebi were sometimes erected in several points within the royal compound. Beside, daebi's residence in this period had additional spaces for ceremonies since they had a number of official events there. Construction of daebi's residences in this era was not confined to the symbolic institutions and they became the peculiar palaces with specific characteristics for official ceremonies of the queen mothers. Consequently, it could be said that the architectural style of dongjo, which was the place of the supreme female in the hierarchical order, stemmed from donggwol where daebi spent the longest time of the royal life.