• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.41 no.3
• /
• pp.191-197
• /
• 2017

In this study, the visualization of the unsteady flow field of a Weis-Fogh-type water turbine was investigated using particle-image velocimetry. The visualization experiments were performed in a parameter range that provided relatively high-efficiency wing conditions, that is, at a wing opening angle ${\alpha}=40^{\circ}$ and at a velocity ratio of the uniform flow to the moving wing U/V = 1.5~2.5. The flow fields at the opening, translational, and closing stages were investigated for each experimental parameter. In the opening stage, the fluid was drawn in between the wing and wall at a velocity that increased with an increase in the opening angle and velocity ratio. In the translational stage, the fluid on the pressure face of the wing moved in the direction of the wing motion, and the boundary layer at the back face of the wing was the thinnest and had a velocity ratio of 2.0. In the closing stage, the fluid between the wing and wall was jetted at a velocity that increased as the opening angle decreased; however, the velocity was independent of the velocity ratio.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
• /
• 2008.04a
• /
• pp.101-108
• /
• 2008

Polymer electrolyte membrane (PEM) fuel cells are promising power generation devices which are ideal for residential and automobile applications, thanks to their fast transient characteristics. However, liquid water produced in PEM fuel cells should be properly managed to enhance the performances and durabilities of the cells. In this study, a visualization experiment was conducted to investigate the flow behavior of water droplets in cathode channels. The visualization experiment was done with four different model flow channels which were made by varying the material (Acrylic and Teflon) and the channel width (1 mm and 2 mm). Acrylic is hydrophilic (contact angle is about $80^{\circ}$) while Teflon is hydrophobic (contact angle is about $120^{\circ}$). A computational fluid dynamics (CFD) analysis was also performed to compare the observed and the simulated two-phase water/air flow characteristics in cathode channels. The computational models were made to be consistent with the geometries and surface properties of the model flow channels. Both the experimental and numerical results showed that the Teflon cathode channel with 1 mm width has the best water management performance among four model flow channels considered. A close correlation was found between the experimental visualization results and the numerical CFD simulation results.

• Applied Microscopy
• /
• v.20 no.2
• /
• pp.57-70
• /
• 1990

Characterization and electron microscopic visualization of the plasmid and the gene expression of Escherichia coli were carried out. Transcriptional units of active structural genes were observed after lysis of Escherichia coli cells. The ribosomes attached to the E. coli genome on mRNA molecule as polyribosomes. From this gradient of polyribosome length, we estimated location of mRNA synthesis initiation site. In this experiment, a granule is ofen present which may correspond to a RNA polymerase at the promoter site. pOX1, pOX7, pOX7A, $pOX7{\Delta}1$, pSTP36, pSTP21, pBR322, and pJH12 were visualized by way of electron microscope, and their estimated sizes were determined to be $5.70{\pm}0.08{\mu}m,\;2.15{\pm}0.10{\mu}m,\;2.14{\pm}0.12{\mu}m,\;7.39{\pm}0.08{\mu}m,\;4.03{\pm}0.04{\mu}m,\;1.50{\pm}0.03{\mu}m\;and\;1.25{\pm}0.09{\mu}m$ respectively. One micrometer of measured length corresponded to about 3.0 Kb. Mica-press adsorption method that allows selectivs visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids. The released plasmid DNA was adsorbed preferently on mica in a divalent cation-free solution. Miller chromatin-spreading method was useful to observe the plasmid and transcripts. BAC method and cytochrome C monolayer were useful to observe the plasmid DNA. Our ability to visualize ultrastructural aspects of the expression of E. coli has given us a unique tool with which to study the regulation the level of an individual gene.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.39 no.11
• /
• pp.891-896
• /
• 2015

This experimental study investigated the wake flow around an elastically supported circular cylinder. In this study, the Reynolds numbers are varied in the region of $1.4{\times}10^4{\leq}Re{\leq}3.2{\times}10^4$. Under these conditions, we have captured the process of the wake mechanism and the moving path of the vortex by measuring the velocity at each position in the wake around the cylinder. Further, these facts from the wind tunnel test are proved by a flow visualization test through a water channel. From the result, we have arrived at the following conclusions : i) The process (formation${\rightarrow}$growth${\rightarrow}$collapse) of vortex is observed in the wake around the cylinder, ii) The vortex efflux angle is approximately $16^{\circ}{\sim}17^{\circ}$ under the experimental conditions. These angles have no relationship with the velocity change and the existence of flow-induced vibrations of the cylinder, and iii) The moving path of the vortex center is obtained by spectrum analysis of the fluctuating velocity behind the cylinder. These are confirmed by conducting visualization tests.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.30 no.5
• /
• pp.109-117
• /
• 2002

An off-surface flow visualization experiments have been performed to investigate the flow field over a delta wing with the leading edge extension(LEX). The model is a flat wing with $65^{\circ}$ sweepback angle. The free stream velocity is 6.2 m/s, which corresponds to Reynolds number of $4.4\times10^5$ based on the wing root chord. The angle of attack and sideslip angle range from $16^{\circ}\sim28^{\circ}$ and $0^{\circ}\sim-15^{\circ}$, respectively. The visualization technique of using the micro water-droplet and the laser beam sheet enabled to observe the vortical flow structures, which can not be obtained by 5-hole probe measurements. With sideslip angle, the interaction and breakdown of the LEX and wing vortices was promoted in the windward side, whereas, it was suppressed in the leeward side.

• Nano
• /
• v.13 no.11
• /
• pp.1850128.1-1850128.10
• /
• 2018

Glutathione (GSH), the protective agent and reducing agent, has been widely used to prepare gold nanoclusters (GSH-Au NCs) with stable fluorescence properties and negative charge of the surface. Meanwhile, polyethyleneimine (PEI) was used as the modification agent to synthesize magnetic ferroferric oxide nanoparticles ($Fe_3O_4$) with fantastic dispersibility and positive charge of the surface. Based on the electrostatic adsorption force, magnetic nano-$Fe_3O_4@GSH-Au$ NCs core-shell microspheres composed of magnetic $Fe_3O_4$ nanoparticles modified by PEI as the core and GSH-Au NCs as the shell were assembled. The prepared $Fe_3O_4@GSH-Au$ NCs microspheres harbored a uniform size (88.6 nm), high magnetization (29.2 emu/g) and excellent fluorescence. Due to the coordination bond action between Au atom and sulfhydryl (-SH), amino ($-NH_2$), carboxyl (-COOH) in sweat, $Fe_3O_4@GSH-Au$ NCs could combine with latent fingerprints. In addition, $Fe_3O_4@GSH-Au$ NCs with good fluorescence and magnetism could detect fingerprints on various objects. Significantly, the powders were not easy to suspend in the air, which avoided the damage to the health of forensic experts and the fingerprints by only powder contacting. Above all, $Fe_3O_4@GSH-Au$ NCs was successfully applied to the latent fingerprint visualization, which has great potential in forensic science.

• Journal of Sensor Science and Technology
• /
• v.14 no.1
• /
• pp.47-51
• /
• 2005

An active ${\mu}$-mixer is important in Bio-MEMS and ${\mu}$-TAS. The mixing state depends on some kinds of factors including the intensity of ultrasonic radiation. We have visualized the mixing state of the mixing chamber with radiation time and presented the influence of the driving voltage in this research. It will be possible to compare the performances of the ultrasonic radiation parts used in the active ${\mu}$-mixer using this method.

• Journal of the Korean Society of Visualization
• /
• v.1 no.1
• /
• pp.75-81
• /
• 2003

A custom micro-PIV optics assembly has been used to measure the flow fold inside a T-junction of a microchannel. The micro-PIV system consists of microscope objectives of various magnifications, a dichroic cube, and an 8-bit CCD camera. Fluorescent particles of diameters 620 nm have been used with a Nd:YAG laser and color filters. A programmable syringe pump with Teflon tubings were used to inject particle-seeded distilled water into the channel at flow rates of 2.0, 4.0, 6.0 mL/hr. The micro-channels are fabricated with PDMS with a silicon mold, then O$_{2}$ -ion bonded onto a slide glass. Results show differences in flow characteristics and resolution according to fluid injection rates, and magnifications, respectively. The results include PIV data with vector-to-vector distances of 2 $\mu$m with 32 pixel-square interrogation windows at 50$\%$ overlap.

• Journal of the Korean Society of Visualization
• /
• v.1 no.1
• /
• pp.82-91
• /
• 2003

The study of swirl flow has been of technical and scientific interest because it has an internal recirculation field and its tangential velocity is related to the curvature of the streamline. The fluid flow for ducts or elbows of an internal engine has been much studied through numerical methods and experiments, but studies about swirl flow has been insufficient. Using the PIV (Particle Image Velocimetry) method, this study found the time-mean velocity distribution, time-mean turbulent intensity, with swirl and without swirl flow for Re=10,000, 15,000, 20,000, and 25,000 along longitudinal sections and the results appear to be physically reasonable. In addition, axial velocity distribution is compared with that of Jeong's, Kodadadi's and Murakami's. It was found that the highest velocity of swirl and non-swirl flow occurs in the opposite position at the center of a round tube, $\phi$=45$^{\circ}$

• Journal of the Korean Society of Visualization
• /
• v.1 no.2
• /
• pp.38-46
• /
• 2003

An experimental investigation was performed to study the turbulent characteristics of swirling flow a 90$^{\circ}C$ circular tube for Re = 10,000, 15,000 and 20,000. 2D-PIV(Particle Image Velocimetry)technique was employed to measure the fluctuation velocity field. The results include spatial distributions of mean velocity vectors, turbulence intensity and turbulence kinetic energy. The axial and radial turbulence intensities, and kinetic energy profiles show double-peak structures in the inlet region of the 90 degree bend and the profiles are disappeared along the test tube with decaying the swirl intensity.