• Child Health Nursing Research
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• v.24 no.4
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• pp.475-483
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• 2018

Purpose: This study aimed to identify stress levels due to end-of-life care, coping strategies, and psychological well-being among nurses in neonatal intensive care unit, and to investigate the effect of stress levels and coping strategies on their well-being. Methods: A total of 128 nurses in the neonatal intensive care units of general hospitals in B city participated. The data were collected using a self-report questionnaire. The collected data were analyzed using descriptive statistics, the t-test, ANOVA, the Pearson correlation coefficient, and hierarchical regression with SPSS version 22.0. Results: The coping strategy that nurses most often used was seeking social support. The factors affecting the well-being of the participants were wishful thinking, problem-focused coping and seeking social support, in order. Those 3 variables explained 21 % of the total variance in psychological well-being. Problem-focused coping and seeking social support were positively associated with psychological well-being, while wishful thinking showed a negative association. Conclusion: In order to improve the psychological well-being of nurses in neonatal intensive care units, it is necessary to provide nurses with a program to build a social support system and to improve their problem-based coping skills.

• Bulletin of the Korean Chemical Society
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• v.27 no.5
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• pp.718-724
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• 2006

The heterogeneous association behaviour of various concentration binary mixtures of mono alkyl ethers of ethylene glycol with ethyl alcohol were investigated by dielectric measurement in benzene solutions over the entire concentration range at 25 ${^{\circ}C}$. The values of static dielectric constant $\epsilon_0$ of the mixtures were measured at 1 MHz using a four terminal dielectric liquid test fixture and precision LCR meter. The high frequency limiting dielectric constant $\epsilon_\infty$ values were determined by measurement of refractive index $n_D$ ($\epsilon_\infty\;=\;n_D\;^2$). The measured values of $\epsilon_0$ and $\epsilon_\infty$ were used to evaluate the values of excess dielectric constant $\epsilon^E$, effective Kirkwood correlation factor $g^{eff}$ and corrective correlation factor $g_f$ of the binary polar mixtures to obtain qualitative and quantitative information about the H-bond complex formation. The non-linear behaviour of the observed $\epsilon_0$ values of the polar molecules and their mixtures in benzene solvent confirms the variation in the associated structures with change in polar mixture constituents concentration and also by dilution in non-polar solvents. Appearance of the maximum in $\epsilon^E$ values at different concentration of the polar mixtures suggest the formation of stable adduct complex, which depends on the molecular size of the mono alkyl ethers of ethylene glycol. Further, the observed $\epsilon^E$ < 0 also confirms the heterogeneous H-bond complex formation reduces the effective number of dipoles in these polar binary mixtures. In benzene solutions these polar molecules shows the maximum reduce in effective number of dipoles at 50 percent dilutions. But ethyl alcohol rich binary polar mixtures in benzene solvent show the maximum reduce in effective number of dipoles in benzene rich solutions.

• BMB Reports
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• v.28 no.3
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• pp.243-248
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• 1995

Cholesteryl ester transfer protein (CETP), a hydrophobic glycoprotein promoting transfer of cholesteryl esters (CE) from high-density lipoproteins (HDL) to lower-density lipoproteins in the plasma, has been recognized a potent atherogenic factor during the development of coronary artery diseases. This study demonstrated a possible utilization of antisense RNA to inhibit expression of the CETP gene using vaccinia virus as an expression system. The CETP cDNA was inserted into a transfer vector (pSC11) in sense and antisense orientations and used to generate recombinant viruses. Recombinants containing sense or antisense orientations of the CETP cDNA were isolated by $TK^-$ selection and X-gal test. The inserted CETP cDNAs in the recombinants were identified by Southern blot analysis and allowed to transcribe in host cells (CV-1). Expressions of the exogenous CETP mRNA, extracted from the CV-1 cells coinfected with viruses containing sense and antisense DNAs, were monitored by Northern blot analysis using the CETP cDNA probe, by Western blot analysis using monoclonal antibody against the C-terminal active region of the CETP and by the CETP assay. Decreased expressions of the exogenous CETP cDNA were clearly evident in the Northern and Western blot analyses as the dose of antisense expression increased. In the CETP assay, the CETP activities decreased compared to the activity obtained from the cell extracts infected with sense construct only.

• Korean Journal of Health Education and Promotion
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• v.25 no.3
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• pp.95-109
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• 2008

Objectives: The study was conducted to investigate visual display terminal(VDT) and related risk factors in office workers. Method: The study subjects were 539 officers at 3 places of business in Gwangju. The data were collected by self-administered questionnaire between October 12 and October 17, 2007. and they agreed to participate in this study. The tool was developed by Moon, Jaedong(1991) for VDT syndrome. Data analysis by using SPSS/win 10.0 was performed with descriptive statistics, t-test and ANOVA. Results: The summary of results is as follows: 1) The mean score of total VDT syndrome was $1.17{\pm}.65$ of full score 4.00, which was lower than other research. 2) For relation between general characteristics and total VDT, sex (t=-5.777, p=.000), age (F=3.516, p=.015), satisfaction of job (F=9.540, p=.000), self-awareness of health (F=25.015, p=.000) had statistically significant difference. 3) For relation between behavioral work factors and total VDT, break time of work(t=-5.363, p=.000), eye movement during computer work(t=2.176, p=.031), wrist exercise during(t=2.337, p=.020), distance between monitor and eyes(F=3.682, p=.012) had statistically significant difference. 3) For relation between environmental work factors and total VDT, height of chair(F=8.801, p=.000), space under desk(F=4.244, p=.015), reflection of monitor(t=2.697, p=.008) had statistically significant difference. Conclusion: To prevent and relieve VDT syndrome and musculoskeletal disorders in office workers, it is important to teach good posture and stretching exercise during work.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.5
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• pp.510-518
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• 2010

In this paper, the designed and implemented results of CFL linearization chip which can be used in mobile radio and TRS terminal of UHF band(380~910 MHz), using $0.6\;{\mu}m$ BiCMOS process based on Si, are shown. As gain control circuits for modifying transmit power are inserted not only in feedback path but also in forward path, the stability of CFL is maintained. And, DC-offset correction function of S/H structure, which is suitable for walkie-talkie PTT operation and is easily implemented, is realized. The performance test results of transmitter show that the regulation of FCC emission mask at PEP 3 W(34.8 dBm) is satisfied when the CQPSK modulated signal is fed and more than 30 dBc improvement of 3rd order IMD is achieved when two-tone signal is inputted.

• Journal of Advanced Navigation Technology
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• v.20 no.4
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• pp.305-313
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• 2016

International Civil Aviation Organization (ICAO) has recommended the adoption of performance-based navigation (PBN), which utilizes global navigation satellite system (GNSS). As a part of efforts to adopt PBN in South Korea, preparations have been made to implement GNSS. In Oct. 2014, Korea augmentation satellite system (KASS) was officially launched for development. A set of navigation devices need to be on-board for an airplane to utilize GNSS. GNSS navigation devices are used for different phases of flights through en-route, terminal, departure, approach and a wide variety of specification standards have been proposed for GNSS navigation. In this paper, we investigate the many proposed standards for GNSS navigation devices and their interfaces. This paper can be useful for designing procedures and flight test used in KASS implementation.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.8
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• pp.1615-1622
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• 2009

Generally, the Terminal Based Navigation System(TBNS) used embedded road data searches a path that has less qualitative than The Center Based Navigation System(CBNS). TBNS has not used real time road data but it is recently able to use it with technique such as TPEG. However, it causes to increase a cost of exploring by using real time road data for improvement quality of a path, because of limited performance. In this paper, we propose a Dynamic Heuristic to improve quality of path in the TBNS. Dynamic Heuristic(DH) is not fixed data and is dynamically modified using transferred real time road data from server. In this paper, we propose path-lading algorithm with Maximum Speed Dynamic Heuristic (DH-MAX) and do an experiment. The DH-MAX is to be used the highest speed as DH, in real map divided by same size. And proposed algorithm searches path using the priority searching only of the fixed data, but also the highest speed with real time information. In the performance test, the quality of path is enhanced but the cost of searching is increased than A* algorithm.

• Biomedical Science Letters
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• v.2 no.1
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• pp.21-30
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• 1996

MAP kinases are a family of serine/threonine specific protein kinases becoming activated in response to different proliferative stimuli by phosphorylation at both threonine and tyrosine residue. Present study shows that MAP kinase was purified from P388 murine leukema cells by SP sephadex C-50, phenyl superose and Mono Q column chromatography and identified with anti-ERKl antibody by western blotting. Immnublotting analysis to the crude extract of P388 cell lysate shows 44 kD and other minor bands but partial purified fraction eluted from phenyl supherose column have 44kD and 66 kD isoform. Subcloned GST-fusion protein from N-terminal of $p56^{kk}$ was tested as a substrate for MAP kinase phosphorylation. It was showed that the wild type and mutant forms(S42A) were fully phosporylated by purified MAP kinase fraction as com-pare with the other mutant form(S59A). This finding suggest that those GST-fusion proteins may be used as substrate for the in vitro test of MAP kinase.

• Toxicological Research
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• v.16 no.4
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• pp.302-309
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• 2000

The purpose of this study is to investigate toxic effects of iso-butylalcohol (iBA) in Sprague-Dawley (SD) rats under the exposure of 6 hours a day, 5 days a week for 13 weeks by inhalation, and to evaluate the occupational safety of iBA in comparison with the permissible exposure level (PEL) stipulated by the Occupational Safety and Health Administration (OSHA). iBA did not induce any abnormal changes from the aspects of clinical signs, feed consumption, ophthalmic test, urinalysis, hematology and blood chemistry during and at the terminal of the inhalation toxicity tests. We did not find any abnormal findings in the gross and microscopic observations due to the inhalation of iBA. There was no alteration in relative organ weights by the inhalation of iBA. No observed adverse effect level (NOAEL) of iBA was considered to be more than 3,000 ppm in rats under the inhalation of 6 hours a day, 5 days a week for 13 weeks. Fifty ppm of iBA, the PEL regulated by OSHA, is too conservative for working places. As iBA showed no abnormal observations in all the experimental parameters at any concentration under this experimental condition, we suggest that 150 ppm is safe enough for the PEL of iBA in the working areas, even taking into onsideration that OSHA lowered the PEL to 50 ppm for fear of the probable risk of its skin irritation.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.3
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• pp.241-248
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• 2007

To clarify the growth mechanisms of thyroid tumors, we investigated apoptotic cells in 88 thyroid tumors, consisting of 24 adenomas, 58 papillary thyroid carcinomas, and 6 undifferentiated carcinoma, using terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate digoxigenin-nick end labeling (TUNEL). The cell proliferating marker was also evaluated immunohistochemically using the monoclonal antibody to Ki-67 antigen (MIB-1) in the same tumors. The apoptosis was expressed as a percentage of the TUNEL-positive cells in the tumor cells, and a proliferating marker, being the percentage of Ki-67 positive cells, was counted up each tumor. The statistical analysis were used analysis of variance (ANOVA) and student's t-test that were analyze the differences in the rate of each histological types of the thyroid tumors. The overall level of apoptosis was extremely low in all histological types of the thyroid tumors analyzed, the mean apoptosis being $0.31{\pm}0.40$ in adenoma, $0.55{\pm}0.48 $in papillary thyroid carcinoma, and $4.60{\pm}3.27$ in undifferentiated carcinoma. The Ki-67 protein in the thyroid tumor subtypes was significantly lower in adenoma and papillary carcinoma, at $2.45{\pm}2.99$ and $6.27{\pm}4.42$, respectively, than that in undifferentiated carcinoma at $26.47{\pm}23.88$ (p<0.0001). There was no correlation between clinicopathological factors and apoptosis or Ki-67 in papillary thyroid carcinoma. In conclusion, our findings suggest that apoptosis occurs infrequently in thyroid tumor, and that cell proliferating maker Ki-67 markedly differs according to the thyroid tumor subtypes. Moreover, the ratio between proliferating cells and apoptotic cells may reflect thyroid tumor progression.