• Title/Summary/Keyword: Test procedures

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Comparative Study of Target Genes and Protocols by Country for Detection of SARS-CoV-2 based on Polymerase Chain Reaction (PCR) (중합효소 연쇄반응 기반의 코로나-19 바이러스 검출법에 대한 국가별 목표 유전자 및 프로토콜 비교 연구)

  • Kim, Jin-Hee
    • The Journal of the Korea Contents Association
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    • v.21 no.1
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    • pp.465-474
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    • 2021
  • Corona-19, a disease caused by 'Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)', was declared a global pandemic by the World Health Organization (WHO) in March 2020, and a real-time polymerase chain reaction test is performed as a diagnostic test for screening and confirmation in most countries. However, not only the target genes and protocols differ by countries, but also the procedures for reading the diagnosis results are diverse, so the criteria for confirmed patients differ by country. Therefore, in this review, we discussed the target genes, test techniques, and diagnostic criteria for each country notified by WHO. And the specificity and sensitivity, limits of detection, positive and negative controls, false positive bacteria candidates, and specimens, and the specifics of the control setting were also described. In addition, the characteristics of Korea's test were compared to each country's one. Finally, in order to obtain the same diagnosis result for SARS-CoV-2 in the future, standardized diagnosis methods and result interpretations for Corona-19 diagnosis were proposed.

Treatment of multiple gingival recessions with xenogeneic acellular dermal matrix compared to connective tissue graft: a randomized split-mouth clinical trial

  • Vincent-Bugnas, Severine;Laurent, Jonathan;Naman, Eve;Charbit, Mathieu;Borie, Gwenael
    • Journal of Periodontal and Implant Science
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    • v.51 no.2
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    • pp.77-87
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    • 2021
  • Purpose: The aim of this study was to compare the efficacy of the tunnel technique for root coverage using a new xenogeneic acellular dermal matrix vs. connective tissue grafting (CTG) for the treatment of multiple maxillary adjacent recessions (recession type 1) at 12 months postoperatively. Methods: This study enrolled 12 patients with at least 3 contiguous, bilateral, symmetrical maxillary gingival recessions (i.e., at least 6 recessions per patient). In total, 74 recessions were treated using the modified coronally advanced tunnel (MCAT) technique combined with a novel porcine-derived acellular dermal matrix (PADM) at 37 test sites or CTG at 37 control sites. The following clinical parameters were measured: recession height, clinical attachment level, width of keratinized tissue, probing depth, recession width, gingival thickness, mean root coverage (MRC), and complete root coverage (CRC). Comparisons between test and control groups were made for pain visual analog scale scores at 14 days. Results: At 12 months, the MCAT with PADM (test) yielded a statistically significant improvement in all clinical parameters studied. MRC was significantly higher on the control sides (80.6%±23.7%) than on the test sides (68.8%±23.4%). Similarly, CRC was 48.7%±6.8% on the control sides (CTG), in contrast to 24.3%±8.2% on the test sides (PADM). Statistically significant differences were observed in favor of the control sides for all clinical parameters studied. Nevertheless, the MCAT in adjunction with PADM was clearly superior at reducing mean and maximum patient-reported postoperative pain intensity and pain duration in the first week after surgery. Conclusions: The use of PADM to treat multiple recessions improved clinical parameters at 12 months, but these outcomes were nevertheless poorer than those observed for CTG. However, PADM reduced morbidity, particularly the pain experienced by patients.

Prediction of Draft Force of Moldboard Plow according to Travel Speed in Cohesive Soil using Discrete Element Method (이산요소법을 활용한 점성토 환경에서의 작업 속도에 따른 몰드보드 플라우 견인력 예측)

  • Bo Min Bae;Dae Wi Jung;Dong Hyung Ryu;Jang Hyeon An;Se O Choi;Yeon Soo Kim;Yong Joo Kim
    • Journal of Drive and Control
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    • v.20 no.4
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    • pp.71-79
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    • 2023
  • In the field of agricultural machinery, various on-field tests are conducted to measure design load for optimal design of agricultural equipment. However, field test procedures are costly and time-consuming, and there are many constraints on field soil conditions due to weather, so research on utilizing simulation to overcome these shortcomings is needed. Therefore, this study aimed to model agricultural soils using discrete element method (DEM) software. To simulate draft force, predictions are made according to travel speed and compared to field test results to validate the prediction accuracy. The measured soil properties are used for DEM modeling. In this study, the soil property measurement procedure was designed to measure the physical and mechanical properties. DEM soil model calibration was performed using a virtual vane shear test instead of the repose angle test. The DEM simulation results showed that the prediction accuracy of the draft force was within 4.8% (2.16~6.71%) when compared to the draft force measured by the field test. In addition, it was confirmed that the result was up to 72.51% more accurate than those obtained through theoretical methods for predicting draft force. This study provides useful information for the DEM soil modeling process that considers the working speed from the perspective of agricultural machinery research and it is expected to be utilized in agricultural machinery design research.

Determination of Formaldehyde in Cosmetics Using a Convenient DNPH Derivatization Procedure Followed by HPLC Analysis (간편한 DNPH 유도체화 HPLC 분석법을 이용한 화장품 중 포름알데하이드 분석)

  • Choi, Jongkeun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.1
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    • pp.65-73
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    • 2016
  • Korea Food and Drug Administration (KFDA) has officially announced 2,4-dinitrophenylhydrazine (DNPH) derivatization - high performance liquid chromatography (HPLC) methods for analysis of formaldehyde. This study was conducted to develop a convenient derivatization method for cosmetics by improving complex pre-treatment procedures included in KFDA method. To simplify pre-treatment procedures of KFDA method, reaction conditions including pH, time and temperature were optimized. This pre-treatment method does not require complicate pre-treatment steps of KFDA method such as pH adjustment of test solution with acetate buffer (pH 5.0), solvent-solvent partitioning with dichloromethane and concentrating procedure with vacuum evaporator. Formaldehyde-dinitrophenylhydrazone (formaldehyde-DNP) product produced by derivatization reaction was separated and quantified with a reversed-phase HPLC, which was slightly modified with KFDA method. The linearity test showed good results with 0.9999 of correlation coefficient ($r^2$) in the range of 2 ~ 40 ppm of standard solutions. In this method, limit of detection (LOD) and limit of quantitation (LOQ) values for formaldehyde were 0.2 ppm and 0.5 ppm, respectively. In addition, recovery test demonstrated that the method was also accurate and reproducible. Therefore, the proposed method can be applicable to rapid analysis of formaldehyde in cosmetics.

A Correlation Study between the Environmental, Personal Exposures and Biomarkers for Volatile Organic Compounds (대기 중 휘발성유기오염물질의 환경, 개인 및 인체 노출의 상관성 연구)

  • Jo, Seong-Joon;Shin, Dong-Chun;Chung, Yong;Breysse, Patrick N.
    • Environmental Analysis Health and Toxicology
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    • v.17 no.3
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    • pp.197-205
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    • 2002
  • Volatile organic compounds (VOCs) are an important public health problem throughout the world. Many important questions remain to be addressed in assessing exposure to these compounds. Because they are ubiquitous and highly volatile, special techniques must be applied in the analytical determination of VOCs. Personal exposure measurements are needed to evaluate the relationship between microenvironmental concentrations and actual exposures. It is also important to investigate exposure frequency, duration, and intensity, as well as personal exposure characteristics. In addition to air monitoring, biological monitoring may contribute significantly to risk assessment by allowing estimation of absorbed doses, rather than just the external exposure concentrations, which are evaluated by environmental and personal monitoring. This study was conducted to establish the analytic procedure of VOCs in air, blood, urine and exhaled breath and to evaluate the relationships among these environmental media. The subjects of this study were selected because they are occupationally exposed to high levels of VOCs. Environmental, personal, blood, urine and exhalation samples were collected. Purge & trap, thermal desorber, gas chromatography and mass selective detector were used to analyze the collected samples. Analytical procedures were validated with the“break through test”, 'quot;recovery test for storage and transportation”,“method detection limit test”and“inter-laboratory QA/QC study”. Assessment of halogenated compounds indicted that they were significantly correlated to each other (p value < 0.01). In a similar manner, aromatic compounds were also correlated, except in urine sample. Linear regression was used to evaluate the relationships between personal exposures and environmental concentrations. These relationships for aromatic and halogenated are as follows: Halogen $s_{personal}$ = 3.875+0.068Halogen $s_{environmet}$, ($R^2$= .930) Aromatic $s_{personal}$ = 34217.757-31.266Aromatic $s_{environmet}$, ($R^2$= .821) Multiple regression was used to evaluate the relationship between exposures and various exposure deter-minants including, gender, duration of employment, and smoking history. The results of the regression model-ins for halogens in blood and aromatics in urine are as follows: Halogen $s_{blood}$ = 8.181+0.246Halogen $s_{personal}$+3.975Gender ($R^2$= .925), Aromatic $s_{urine}$ = 249.565+0.135Aromatic $s_{personal}$ -5.651 D.S ($R^2$ = .735), In conclusion, we have established analytic procedures for VOC measurement in biological and environmental samples and have presented data demonstrating relationships between VOCs levels in biological media and environmental samples. Abbreviation GC/MS, Gas Chromatography/Mass Spectrometer; VOCs, Volatile Organic Compounds; OVM, Organic Vapor Monitor; TO, Toxic Organicsapor Monitor; TO, Toxic Organics.

A Study on the Static Structural Strength Evaluation of 53ft Liquefied Natural Gas Tank Container (53ft 액화천연가스 탱크 컨테이너의 정적 구조 강도 평가에 관한 연구)

  • Chunsik Shim;Hokyung Kim;Daseul Jeong;Deokyeon Lee;Kangho Kim;Minsuk Kim;Sungkuk Wi;Heechang Noh;Youngbin Kwon;Changseok Hong;Kim Byeonghwa;Cheonghak Kim
    • Journal of the Society of Naval Architects of Korea
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    • v.60 no.4
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    • pp.278-287
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    • 2023
  • This study aims to analyze and evaluate the structural strength of a 53ft Liquefied Natural Gas (LNG) tank container according to International Organization for Standardization (ISO) 1496-3, amidst growing global demand for LNG transportation. The research was conducted in two main stages: structural analysis using Finite Element Analysis (FEA) under various load conditions, and structural strength tests following ISO 1496-3 test procedures. The structural analysis was performed considering different loading conditions to assess the structural safety of the tank container. Calculated stresses were compared with allowable stress under specified load conditions. The structural strength tests were conducted at Mokpo National University's Subsea Umbilical cable Riser Flowline R&D Center, which provided a suitable testing environment. The study found that calculated stresses met the allowable stress under specified load conditions, confirming the structural safety of the tank container. Additionally, the maximum deformation and permanent deformation satisfied the design criteria for all test cases, indicating the container's structural strength meets requirements. The research also contributed valuable data for future structural strength tests of similar products and facilitated the development of safe and efficient LNG transportation solutions by developing effective test procedures in accordance with ISO 1496-3 standards.

Experimental Study for Optimal Method in Measuring the Basic Friction Angle of Rock (최적의 기본마찰각 측정법에 관한 실험적 연구)

  • Lee, Kook-Hyun;Jang, Hyun-Sic;Jang, Bo-An
    • The Journal of Engineering Geology
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    • v.29 no.4
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    • pp.565-578
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    • 2019
  • Basic friction angles of Hwangdeung granite, Berea sandstone, Jeongsun marble, Hongcheon gneiss, Pungam shale and Eumseong sandy shale were measured by direct shear test, tilt test and pull test. Characteristics of basic friction angle and the accuracy of test methods were compared and the optimal method in measuring basic friction angle was suggested. Although basic friction angles might be measured accurately by direct shear test, the test apparatus is expensive and procedures are complicated. Tilt tests which is the suggested method for measuring basic friction angle by International Association for Rock Mechanics also provided similar basic friction angles measured by direct shear test. However, the error measured for the same rock type is higher than 7° and values by repeated measurements in one sample show different trends, such as increasing or decreasing or almost constant as measurements continued. The difference measured in one gneiss sample is higher 12°, indicating that tilt test may be not a reliable method for measuring basic friction angle. Not only pull test provided accurate and consistent results under low normal stresses, but also test apparatus is simple and inexpensive and procedure is not complicated, indicating that pull test may be the optimal method for measuring basic friction angle.

Study on Achievement of Nursing Students-Relationship between Psychological Test Characteristics and Academic Achievement of Nursing Students in a Baccalaureate Program- (간호학생의 학업성취에 관한 연구 -대학 간호학생의 심리적 제특성과 학업성취와의 관계-)

  • 이은옥;이미라
    • Journal of Korean Academy of Nursing
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    • v.3 no.1
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    • pp.53-66
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    • 1972
  • There is an urgent need to improve the tool predicting success or failure of academic achievement of nursing in Korea so as to identify as early as possible those students who should receive special instruction and to improve screening procedures for admission of nursing. The main purpose of this study is to identify the correlation between the grade point averages of courses learned and their psychological test characteristics in a baccalaureate nursing program. All 240 students, except freshmen, enrolled in Nursing Department of Seoul National University in the spring semester, 1972, participated in this study. All of the subjects completed the psychometric tests such as interest test, personality test and test of self-concept. Total grade point averages, grade point averages of general education subjects, of supporting science courses and of professional education subjects were used as performance criteria of the students. Through the calculation of product-moment correlation coefficients between the test scores and four grade point averages of each class and of total subjects, the following findings and recommendations were obtained. 1. There was so much variation in characteristics of interest test correlated with academic achievement of nursing students in each class. 2. Since the school objectives, curriculum and teaching strategies may affect predictive efficiency of characteristics of students'interest test, interest test must-be utilized in a homogeneous group in order to predict school achievement. 3. Characteristics of interest test positively correlated at significant level with total grade point averages of all subjects were scientific interest-biological, scientific interest-physical, and humanitarian interest. Scientific interest-physics: was the only characteristic positively correlated at significant level with total grade point averages and grade point averages of professional courses. 4. There were various patterns in characteristics of personality test correlated with school achievement of nursing students by class pattern and personality maturation as they progress toward higher classes. 5. A characteristic of personality test, responsibility, is in high positive correlation with academic achievement in the upper division of classes. 6. Responsibility was the sole personality factor positively correlated at significant level with total grade point averages and grade point averages of nursing courses in the total number of students. 7. There were very different correlation coefficients between characteristics of self-concept test and academic achievement according to the type of each class and type of courses they learned. 8. Characteristics of self-concept test positively correlated at significant level with total grade point averages and grade point averages of nursing courses of all students were physical self and row variability. Those who have positive concept on their own physical status and who are deficient in self-concept were higher in total grade point averages and grade point averages of professional courses than other students. 9. Scores of professional courses offered in freshmen and sophomore classes were in positive correlation with limited number of characteristics of psychological tests. In pursuit of a tool predicting successful academic achievement of nursing students, their G.P.A. during the junior and senior year of nursing will serve as the more reasonable criteria. 10. Junior students of this school were in higher positive correlation with many psychological factors than other classes.

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The Test Strip Reflectometer Method as a Quick Test Procedure for Soil Nitrate Nitrogen (토양의 질산태 질소 간이검정)

  • Hong, Soon-Dal;Park, Hyo-Taek
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.5
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    • pp.369-375
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    • 2000
  • To find a quick test procedure for soil nitrate concentration, two methods, i.e. "Nitracheck 404" reflectometer (Eijkelkamp, Netherlands) with nitrate test strip and a portable colorimeter "Hanna Ion Specific meter(USA) with a color development reagent, were tested for twenty soils with different nitrate contents ranged between from $10mg\;kg^{-1}$ to $340mg\;kg^{-1}$. The standard deviation, coefficient of variability, and recovery from these quick test procedures were compared with those measured by conventional Kjeldahl distillation method and nitrate ion electrode method. The nitrate concentration measured by portable colorimeter method was higher in soils with low concentration and lower in soils with high concentration than those measured by conventional methods. However, concentrations measured by test strip reflectometer method was showed the similar coefficient of variability and recovery as those by conventional methods for all soil samples. From the experimental results in this study, the test strip reflectometer method was thought to be recommendable one revealed the satisfied results for accuracy, quickness, and simplicity for field test of soil nitrate concentration.

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Calibration Technique of a Gimballed INS by the Change of Schuler Period (슐러주기 변경에 의한 김블형 관성항법장치 교정기법 연구)

  • Sin, Yong-Jin;Kim, Cheon-Jung;Park, Jeong-Hwa
    • Journal of Institute of Control, Robotics and Systems
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    • v.7 no.10
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    • pp.843-848
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    • 2001
  • Most of gimballed inertial navigation systems(GNIS) are calibrated periodically to maintain their inherent accuracy. The existing calibration techniques using the conventional schuler test with the least square method and the multiposition test take a long time and have some problems in procedures. To solve this problem, calibration method using a linear Kalman filter is proposed by us. In this paper, the calibration method by the change of Schuler period is studied in order to improve the calibration performance of the gimballed INS. First of all, it is shown that the observability of Kalman filter is also enhanced the Schuler period is decreased. Simulation results show that the calibration performance using the present scheme is improved according to the decrease of the Schuler period and the calibration time is shortened extremely, too. And our proposed technique shows desirable estimation performance for the g-sensitive errors of inertial sensors in particular.

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