• Title/Summary/Keyword: Test Species

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Selection and Identification of Phytohormones and Antifungal Substances Simultaneously Producing Plant Growth Promoting Rhizobacteria from Microbial Agent Treated Red-pepper Fields (미생물제제시용 고추경작지로부터 식물생장홀몬과 항진균물질을 동시에 생산하는 식물생장촉진근권세균의 선발 및 동정)

  • Jung, Byung-Kwon;Lim, Jong-Hui;An, Chang-Hwan;Kim, Yo-Hwan;Kim, Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.40 no.3
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    • pp.190-196
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    • 2012
  • In this study, a total of more than 1,000 bacteria, including 739 species of aerobic bacteria, 80 species of urease producing bacteria and 303 species of photosynthetic bacteria, were isolated from red-pepper field soils located in the Gyeongsan Province of the Republic of Korea. Amongst these, 158 species of aerobic bacteria, 70 species of urease producing bacteria and 228 species of photosynthetic bacteria were found to be auxin producing soil bacteria through quantification analysis with the Salkowski test. The latter groupings were then tested for antifungal activities to ${\beta}$-Glucanase and siderophore using CMC congo red agar and CAS blue agar media. In addition, the selected strains were examined for antifungal activity against various phytopathogenic fungi on PDN agar media. Six strains; BCB14, BCB17, C10, HA46, HA143, and HJ5, were noted for their ability to both produce auxin and act as antifungal substances. 16S rDNA sequence comparison analyses of these six strains identified them as Bacillus subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, and B. safensis HJ5.

Native Plants Combination for Ecological Environmental Restoration of the Dissected Sloping Area (절개지 사면의 생태환경 복원을 위한 자생식물 조합)

  • Lee, Chang Shook;Suh, Hyoung Min;Kim, Dong Geun;Eum, Sang Mi;Choi, Sun A;Lee, Nam Sook
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.6
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    • pp.36-50
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    • 2009
  • To suggest the native plants combination for restoration of the dissected sloping area, the plant communities of thirty seven quadrats from five localities of mid-southern part of Korean peninsula were surveyed from 2007 to 2008. Their flora, vegetation structure, and dominant species based on the importance value were investigated. And the soil characters (pH, moisture content, water holding capacity, and organic matter) of each dominant species were analyzed. Also, germination tests were performed to check the stability of restored native plants using seed chips. As a result, 79 native plants were suggested for the dissected sloping area : 20 trees and subtrees for the upper layer, 18 shrubs and vines for the middle layer, and 41 herbs for the lower layer, taken together their flora, dominant species, vegetation structure, soil condition, germination test, flowering period.

Acute Toxicity of Heavy Metals, Tributyltin, Ammonia and Polycyclic Aromatic Hydrocarbons to Benthic Amphipod Grandidierella japonica

  • Lee, Jung-Suk;Lee, Kyu-Tae;Park, Gyung-Soo
    • Ocean Science Journal
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    • v.40 no.2
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    • pp.61-66
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    • 2005
  • Benthic amphipod, Grandidierella japonica widely inhabits the Korean coastal waters and is developed as a standard test species for sediment toxicity tests. We exposed G. japonica to various pollutants including 4 kinds of inorganic metals (Ag, Cd, Cu and Hg), tributyltin [TBT], ammonia and 7 polycyclic aromatic hydrocarbon (PAH) compounds (acenaphthene, chrysene, fluoranthene, fluorene, naphthalene, phenanthrene and pyrene) to estimate the no observed effect concentration (NOEC) and the median lethal concentration (LC50) of each pollutant during the 96-hour acute exposure. Among all tested pollutants, TBT was most toxic to G. japonica, and Rg was most toxic among inorganic metals. The toxicity of pyrene to G. japonica was greatest among PAH compounds, followed by fluoranthene, phenanathrene, acenaphthene, fluorene and naphthalene. The toxicity of PAH compounds was closely related to their physico-chemical characteristics such as $K_ow$ and water solubility. G. japonica responded adequately to pollutant concentrations and exposure durations, and the sensitivity of G. japonica to various inorganic and organic pollutants was generally comparable to other amphipods used as standard test species in ecotoxicological studies, indicating this species can be applied in the assessment of environments polluted by various harmful substances.

Development of Non-Invasive Fecal PCR Assay for Detecting the Helicobacter Species Infection in Dogs (개의 Helicobacter 균속 감염 진단을 위한 비 침습적 분변 PCR 분석법)

  • Cheol-Yong Hwang;Hwa-Young Youn;Hong-Ryul Han
    • Journal of Veterinary Clinics
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    • v.19 no.3
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    • pp.295-298
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    • 2002
  • This study was conducted to develope noninvasive fecal PCR assay for detecting the Helicobacter species in dogs. From the DNA isolated from fecal samples, and a region of the 16S rRNA gene conserved among Helicobacter spp. was amplified In comparison with gastric biopsy test, the fecal PCR assay showed high specificity(100%) and sensitivity(96%). The prevalence of Helicobacter spp. infection in privately owned pet dogs in Korea detemined by the fecal PCR assay was 72.1%. the fecal PCR assay determined in this study can a new noninvasive test detecting Helicobacter spp. infection in dogs.

Efficiency of concentrating marine microplanktonic organisms using net sampler to verify the efficacy of a ship's ballast water treatment system (USCG phase-II 선박평형수 처리장치 성능 평가에 대비한 해양식물플랑크톤 네트 농축효율 비교)

  • Baek, Seung Ho;Lee, Min Ji;Shin, kyoungsoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.3
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    • pp.136-143
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    • 2016
  • To provide a type approval test for Ballast Water Treatment System (BWTS) of United States Coast Guard (USCG) Phase-II, this study examined the concentrating efficiency of nets for ${\geq}10{\mu}m$ and ${\leq}50{\mu}m$ sized phytoplanktonic organisms using different mesh sized nets ($5{\mu}m$ or $7{\mu}m$), different injection methods (hand breaker as semi-continuous assessment or pump as continuous assessment), and different filterability for the water volume. As a result of the t-test, the net concentrated efficiency between $5{\mu}m$ and $7{\mu}m$ mesh size was not significant (p > 0.05). The difference in the net concentrated efficiency for filtered natural water volume was not significant (p > 0.05). On the other hand, the Chl.a concentration in the continuous water injection method was significantly (p < 0.05) higher than that of semi-continuous water injection (t-test: t: -4.058). In the natural phytoplankton community, a total of 36 species were identified, including Bacillariophyta (17 species), Dinophyta (15 species), Euglenophyta (1 species), Dictyochophyta (2 species), and unidentified taxa (1 species). Among them, diatom Pseudo-nitzchia spp. was remarkably dominant. In particular, the net concentrated efficiency in all assessments was underestimated to be approximately 20-25%, which was caused by the small size Pseudo-nitzchia spp.. A width size of these genus might have passed through the $5{\mu}m$ or $7{\mu}m$ mesh size of the net. Therefore, net concentrated efficiency is dependent on the size of the observed species in natural water. This issue should be considered when determining the net volume for the type approval test of BWTS.

A new species of Parastenocaris from Korea, with a redescription of the closely related P. biwae from Japan (Copepoda: Harpacticoida: Parastenocarididae)

  • Karanovic, Tomislav;Lee, Wonchoel
    • Journal of Species Research
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    • v.1 no.1
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    • pp.4-34
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    • 2012
  • Parastenocaris koreana sp. nov. is described based on examination of numerous adult specimens of both sexes from several localities in Korea. Scanning electron micrographs are used to examine intra- and interpopulation variability of micro-characters, in addition to light microscopy. The new species is most closely related to the Japanese P. biwae Miura, 1969, which we redescribe based on newly collected material from the Lake Biwa drainage area. The two species differ in size, relative length of the caudal rami, shape of the anal operculum, shape of the genital double somite, relative length of the inner distal process on the female fifth leg, as well as relative length of the apical setae on the second, third, and fourth legs exopods in both sexes. Detailed examinations of three disjunct populations of P. koreana reveal also some geographical variation, especially in the surface ornamentation of somites, which may indicate some population structuring or even cryptic speciation. Lack of intraspecific variability in the number and position of sensilla on somites, as well as their potential phylogenetic significance, is a novel discovery. Both species examined here belong to the brevipes group, which we redefine to include 20 species from India (including Sri Lanka), Australia, East Asia, Northern Europe, and North America. A key to species of this group is also provided. In order to test the monophyly of the redefined brevipes group with highly disjunct distribution, as well as relationship between different species, a cladistics analysis is performed based on 39 morphological characters and with help of three outgroup taxa. Six equally parsimonious cladograms are generated, all of which show that the ingroup is well defined by at least three synapomorphies. Reconstructed phylogeny questions the previously suggested hypothesis about the origin of this group in South East Asia, with one Australian species showing the most basal position. We speculate that the present distribution of this group may be a combination of ancient vicariance and subsequent dispersal, with a possible origin in the Gondwanaland, in the rift valley between Australia and India.

Leaf Feeding Insects of Welsh Onion and Shallot, and Their Species Abundance Patterns (대파 및 쪽파 잎기생 해충상과 종 빈도 분포형)

  • 고현관;최재승;엄기백;최귀문;김정화
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.360-365
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    • 1992
  • Leaf feeding insect species of welsh onion and shallot were surveyed, and their species abundances were analyzed by fitness test for lognomal disstribution. A total of 13 and 6 species were identified on welsh onion and shallot, respectively. The dominant species on welsh onion were Thrips tabacid, Acrolepiopsis sapporensis, Spodoptera exigua, and Liriomyza chinensis. Thrips tabacid was also identified as the major species on the shallot. The community dominance was high in welsh onion and shallot observed on 12 October, Suwon. The species abundance patterns of the two communities were well described by lognormal distribution(P> 0.50).

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Study on Durability of Wood Deck according to Species (수종별 목재 데크재의 내구성에 관한 연구)

  • Kim, Kyoung Jung;Lee, Won Jae;Choi, Chul;Kim, Hee Jin;Kang, Seog Goo
    • Journal of the Korea Furniture Society
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    • v.28 no.2
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    • pp.111-117
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    • 2017
  • Recently, as people's interest in wood has increased, the use of wood as household and landscape decking materials has increased. As the deck material, imported wood such as synthetic wood, Ipe, and Malas was used in addition to the existing preserved wood, but recently deck use has been activated as part of the activation of domestic materials. As an important quality factor in the selection of such decking materials, various durability along with weatherability for long - term use is required for maintenance. Generally used tropical hardwoods have excellent weatherability and durability without additional preservative treatment. However, the domestic larch is a wood species with a higher specific gravity and durability than ordinary conifers. However, it has not yet been used as a deck material due to lack of comparative studies on its characteristics. Therefore, hardness and durability of wood were measured using six specimens of Ipe, Massaranduba, Malas, Douglas-fir, Larch and Torrefied-Larch. Density Profile was used to measure the density, and Brinell hardness test and resistance test against momentary impact were carried out for the test of resistance to static load. Also, The hardness and durability of wood were measured by castor test with resistance test against dynamic load, as well as, nail down test by experiment on surface hardness and durability. As a result of the experiment, the hardness was increased in proportion to the density, and it was confirmed that the imported lumber was harder and durable than the domestic larch.

Analysis of Healthcare Convergence on Bacterial Contamination of Radiological Equipment in Emergency Rooms of General Hospitals (종합병원 응급실내의 영상의학과 장비에서의 세균 오염에 관한 보건학적 융합 분석)

  • Hong, Dong-Hee;Park, Mi-Soon
    • Journal of the Korea Convergence Society
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    • v.8 no.1
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    • pp.51-59
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    • 2017
  • It is to establish preliminary data about healthcare convergencr of infection control by performing a bacterial contamination test for CT scanner as well as general radiographic systems in the emergency rooms of general hospitals. The period was December 1 ~ December 31, 2015. It is detect in the emergency rooms of 7 medical facilities including 2 in Seoul, and 1 in Gyeonggi-do Province, and 2 in Jeonla-do Province, and 2 in Chungcheong-do Province. The surface contamination strains detected from CT scanner in the radiology department emergency rooms were Micrococcus species(4,5%), Stenotrophomonas maltophilia(9%), Enterococcus faecium(4.5%), Providencia stuartii(4.5), and Gram negative bacilli(4.5%). Also, the surface contamination strains detected from general radiographic system in the radiology department emergency rooms were Providencia stuartii(11%), Klebsiella pneumonia(3.5%), Stenotrophomonas maltophilia(11%), Pantoea species(11%), Acinetobacter baumannii(3.5%), Micrococcus species(3.5%), Escherichia coli(3.5%), Enterobacter species(3.5%), and Gram negative bacilli(11%). Considering that the regions of most detection were all the places closely related to patients, radiologists would have to thoroughly clean with alcohol before and after a test.

Studies on the Isolation of Streptococcal species Causing Bovine Mastitis and Susceptibility Test to Them (젖소 유방염 유즙에서 분리된 연쇄상구균속의 분류와 약제감수성에 관한 연구)

  • Kim Tae-Jong
    • Journal of the korean veterinary medical association
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    • v.25 no.7
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    • pp.423-433
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    • 1989
  • The bovine mastitis investigated with 910 cows of 44 dairy farms in the area of Seoul and Kyunggi-Do. The streptococcal species were isolated from milk with mastits and their biochemical properties and serological reaction were investigated, and an antimi

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