• Journal of Microbiology and Biotechnology
• /
• 제12권5호
• /
• pp.826-828
• /
• 2002

In our previous studies, we have cloned and characterized a gene region from Bradyrhizobium japonicum ,which is involved in the synthesis of lipopolysaccharide (LPS). In this study, we have expanded the sequence analysis of the region and found an additional open reading frame (orf), which appeared to be divergently transcribed from the rfaF gene. Sequence alignment of the orf revealed a significant similarity with rfaD genes of Salmonella typhimurium , Escherichia coli, and Neisseria gonorrhoeae. These genes encode a heptose-6-epimerase, which catalyzes the interconversion of ADP -D -glycerol-D-manno-heptose to ADP-L-glycero-D-manno-heptose. This divergent organization of the rfaF and rfaD genes is different from that of other Gram-negative bacteria where two genes form an operon. A rfaD- mutant of E. coli was successfully transformed with plasmid constructs containing the rfaD gene of B. japonicum. Novobiocin sensitivity test showed that the rfaD gene from B. japonicum could complement the rfaD mutation in E. coli, which confirms the functionality of the cloned B. japonicum gene.

• Fisheries and Aquatic Sciences
• /
• 제16권4호
• /
• pp.243-251
• /
• 2013

Genetic diversity and gene flow patterns in Pollicipes mitella were investigated with a nucleotide sequence analysis of 514 base pairs from the mitochondrial cytochrome c oxidase subunit I gene (COI) in 124 samples collected from six Korean populations. In total, 59 haplotypes were defined by 40 variable nucleotide sites in the COI region. The haplotypes had shallow haplotype genealogy and no geographic associations. All populations had high haplotype diversity (0.909 to 0.979) and low nucleotide diversity (0.0055 to 0.0098). The haplotypes with recently diverged nucleotides were distributed by long-range larvae dispersal among regional populations. The pairwise fixation indices ($F_{ST}$) estimated with the exact test and migration rates indicate that substantial gene flow has occurred among populations as a result of sea currents, except between the Uljin (East Sea coast) and other Korean populations. This suggests that significant genetic differentiation and low migration rates have affected the Uljin population.

• Journal of Periodontal and Implant Science
• /
• 제41권3호
• /
• pp.131-134
• /
• 2011

Purpose: To test the plaque-removal efficacy of a single-tufted toothbrush on the posterior molars compared with a flat-trimmed toothbrush. Methods: Forty-nine subjects were selected. Professional instruction and written brushing instructions were given. After thorough supra-gingival scaling and polishing, all subjects were asked to abstain from oral hygiene procedures for 24 hours prior to the first experiment. The subjects were randomized to a treatment sequence. The modified Quigley and Hein plaque index was recorded pre- and post-tooth brushing, at 6 surfaces of the posterior molars. After a wash-out period, all the remaining plaque was removed professionally. Twenty-four hours of brushing abstinence was again performed. The plaque index was recorded pre- and post-tooth brushing after the subjects were given the second toothbrush in the cross-over sequence. Results: The percentage reductions in plaque scores achieved with the single-tufted brushes were significantly higher than those of the flat-trimmed brush at the maxillary buccal interproximal, marginal and mandibular lingual interproximal site. The other locations showed no significant difference. Conclusions: The results of the present study implied that the single-tufted brush could be an effective tool for the removal of plaque at some, but not all, sites of the posterior molars.

• Steel and Composite Structures
• /
• 제19권5호
• /
• pp.1221-1236
• /
• 2015

Since the coefficients of thermal expansion (CTE) between concrete and GFRP, steel and GFRP are quite different, GFRP laminates with different laminas stacking-sequence present different thermal behavior and currently there is no specification on mechanical properties of GFRP laminates, it is necessary to investigate the thermal influence on composite girder with stay-in-place (SIP) bridge deck at different levels and on different scales. This paper experimentally and theoretically investigated the CTE of GFRP at lamina's and laminate's level on micro-mechanics scales. The theoretical CTE values of laminas and laminates agreed well with test results, indicating that designers could obtain thermal properties of GFRP laminates with different lamina stacking-sequence through micro-mechanics methods. On the basis of the CTE tests and theoretical analysis, the thermal behaviors of composite girder with hybrid GFRP-concrete deck were studied numerically and theoretically on macro-mechanics scales. The theoretical results of concrete and steel components of composite girder agreed well with FE results, but the theoretical results of GFRP profiles were slightly larger than FE and tended to be conservative at a safety level.

• BMB Reports
• /
• 제38권3호
• /
• pp.350-353
• /
• 2005

Alanine at residue 73 (Ala-73) and aspartate at residue 9 (Asp-9) are characteristic to both Cw6 and Cw7 alleles of HLA-C gene and have been suggested as possible markers for psoriasis vulgaris (PsV). However, the results from various ethnic groups/populations are contradictory and inconclusive. In this study, an attempt has been made to examine the association between HLA-C (Ala-73 and Asp-9) and susceptibility to PsV among Saudi patients. Genomic DNA was extracted from 25 Saudi PsV patients and 75 control subjects. Polymerase chain reaction (PCR) was performed to amplify HLA-C sequences using earlier reported primers, C133P and C243PR. Sequence-specific primers were used to specifically detect nucleotide coding for Ala-73 and Asp-9 in all the subjects. The results showed significantly higher frequency of Asp-9 (84.0% versus 61.3%) in PsV patients as compared to controls (p < 0.05, 2-tailed Fisher's exact test). The frequencies of Ala-73 among PsV patients (92%) and controls (88%) did not differ significantly.

• Mycobiology
• /
• 제43권4호
• /
• pp.435-443
• /
• 2015

Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.

• Food Science and Biotechnology
• /
• 제16권1호
• /
• pp.67-70
• /
• 2007

Mackerel (Scomber japonicus) often causes severe allergic reactions in sensitive people. Food containing undeclared mackerel may pose a risk to such people. The major allergenic protein in fish such as mackerel, codfish, and Alaska pollack has been found to be parvalbumin. In this study, we developed a polymerase chain reaction (PCR) method to detect mackerel DNA using primers corresponding to the parvalbumin gene. We cloned and sequenced 1.5 kb of parvalbumin gene by PCR using mackerel genomic DNA as a template. Nucleotide sequence analysis of genomic parvalbumin gene, composed of 4 exons and 3 introns, allowed the selection of two pairs of oligonucleotide primers specific for mackerel. These primers successfully enabled PCR amplification of specific regions of genomic parvalbumin DNA from mackerel, but no amplification from 8 other fish samples, surimi, and 6 boiled fish pastes. The sensitivity of this method was sufficient to detect 5 ng of purified mackerel DNA mixed with 50 ng of surimi DNA. This rapid and specific method for the detection of allergenic mackerel would be beneficial in reducing food allergy caused by the ingestion of hidden allergen in processed food.

• 한국정보통신학회논문지
• /
• 제14권8호
• /
• pp.1918-1924
• /
• 2010

본 논문은 명암도의 변화가 많은 영상 시퀀스에서 움직임 정보를 효율적으로 추정하는 알고리즘을 제안한다. 기존의 계조도 기반의 움직임 추정 알고리즘은 조명 변화가 심한 시퀀스에서 오류가 많이 발생하지만 제안하는 알고리즘은 장면에서의 밝기 변화를 게인과 오프셋의 선형 모델로 정의하고 각 프레임에서의 그라디언트와 위상 정보를 이용하여 움직임을 정합시키므로 극단적인 상황에서도 강건한 특성을 갖는다. 제안하는 알고리즘을 인위적으로 움직임과 명암 변화를 발생시켜 만든 시퀀스와 플리커가 발생한 실제 동영상 시퀀스에 대해 적용하여 기존의 알고리즘과 성능을 비교하였다.

• 대한음성학회지:말소리
• /
• 제58호
• /
• pp.67-81
• /
• 2006

For a large corpus of time-aligned data, HMM based approaches are most widely used for automatic segmentation, providing a consistent and accurate phone labeling scheme. There are two methods for training in HMM. Flat starting method has a property that human interference is minimized but it has low accuracy. Bootstrap method has a high accuracy, but it has a defect that manual segmentation is required In this paper, a new algorithm is proposed to minimize manual work and to improve the performance of automatic segmentation. At first phase, voiced, unvoiced and silence classification is performed for each speech data frame. At second phase, the phoneme sequence is aligned dynamically to the voiced/unvoiced/silence sequence according to the acoustic phonetic rules. Finally, using these segmented speech data as a bootstrap, phoneme model parameters based on HMM are trained. For the performance test, hand labeled ETRI speech DB was used. The experiment results showed that our algorithm achieved 10% improvement of segmentation accuracy within 20 ms tolerable error range. Especially for the unvoiced consonants, it showed 30% improvement.

• Journal of Microbiology
• /
• 제41권1호
• /
• pp.16-21
• /
• 2003

We have collected eight high-level streptomycin-resistant strains of Pseudomonas marginalis and P. syringae pv. actinidiae which were isolated from kiwifruit orchards in Korea and Japan, The molecular mechanisms of resistance were investigated by the PCR, susceptibility tests, and nucleotide sequence analysis. Of the eight high-level streptomycin-resistant strains, four harbored strA-strB genes, which encode streptomycin-inactivating enzymes. While the three Korean strains of R marginalis did not have plasmid and carried the resistant genes in the chromosomes, the Japanese strain of P. syringae pv. actinidiae had a plasmid containing strA-strB genes. The myomycin susceptibility test demonstrated that the high-level resistance to streptomycin of the remaining four strains is associated with mutations in the rpsL gene. Nucleotide sequence analyses revealed that they contain a single base-pair mutation in codon 43 of their rpsL gene.