• 제목/요약/키워드: Test Sequence

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Null Allele in the D18S51 Locus Responsible for False Homozygosities and Discrepancies in Forensic STR Analysis

  • Eom, Yong-Bin
    • 대한의생명과학회지
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    • 제17권2호
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    • pp.151-155
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    • 2011
  • Short tandem repeats (STRs) loci are the genetic markers used for forensic human identity test. With multiplex polymerase chain reaction (PCR) assays, STRs are examined and measured PCR product length relative to sequenced allelic ladders. In the repeat region and the flanking region of the commonly-used STR may have DNA sequence variation. A mismatch due to sequence variation in the DNA template may cause allele drop-out (i.e., a "null" or "silent" allele) when it falls within PCR primer binding sites. The STR markers were co-amplified in a single reaction by using commercial PowerPlex$^{(R)}$ 16 system and AmpFlSTR$^{(R)}$ Identifiler$^{(R)}$ PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI PRISM$^{(R)}$ 3100 Genetic Analyzer with capillary electrophoresis. The GeneMapper$^{TM}$ ID software were used for size calling and analysis of STR profiles. Here, this study described a forensic human identity test in which allelic drop-out occurred in the STR system D18S51. During the course of human identity test, two samples with a homozygous (16, 16 and 21, 21) genotype at D18S51 locus were discovered using the PowerPlex$^{(R)}$ 16 system. The loss of alleles was confirmed when the samples were amplified using AmpFlSTR$^{(R)}$ Identifiler$^{(R)}$ PCR amplification kit and resulted in a heterozygous (16, 20 and 20, 21) genotype at this locus each other. This discrepancy results suggest that appropriate measures should be taken for database comparisons and that allele should be further investigated by sequence analysis and be reported to the forensic community.

MSCTest: 내장 소프트웨어 테스트를 위한 자동화 도구 (MSCTest: An Automated Testing Tool for Embedded Software)

  • 이남희;서선애;김태효;차성덕;이재원;박기웅
    • 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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    • 제6권2호
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    • pp.187-195
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    • 2000
  • 내장 소프트웨어는 외부 입력과 시스템의 현재 상태를 함께 고려하여 출력을 결정하는 특성을 가지고 있기 때문에, 하나의 모듈을 테스트하기 위해서는 해당 모듈에 대한 단위 테스트 기법 이외에, 추가적으로 그 모듈에 도달하기 위한 다양한 시퀀스를 생성하는 방법이 필요하다. 본 논문에서는 내장 소프트웨어의 모듈 테스트를 위한 명세 기반의 테스트 방법을 제안하고 이를 지원하는 자동화 도구인 MSCTest를 구현하였다. 각 모듈의 기능은 결정표 (decision table)를 이용하여 명세하고, 시스템에 발생가능한 시나리오는 데이타 표현을 첨가한 MSC (Message Sequence Charts)로 기술하여 테스트 시퀀스를 생성한다. MSCTest는 MSC 그래픽 편집기, 테스트 시퀀스와 데이타 생성기, 그리고 생성된 테스트데이타를 수행시키는 테스트 드라이버 생성기로 구성되어 있다. MSCTest를 이용하여 웹 비디오폰이나 디지털 TV 등에서 편리한 사용자 인터페이스를 제공하기 위해 사용되는 EsWin (Embedded System Windows) 소프트웨어를 효과적으로 테스트할 수 있었다.

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태스크 상호작용 테스팅을 위한 MSC 명세로부터의 전체 유한 상태 기계 생성 (Construction of Global Finite State Machine from Message Sequence Charts for Testing Task Interactions)

  • 이남희;김태효;차성덕;신석종;홍인표
    • 한국정보과학회논문지:소프트웨어및응용
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    • 제28권9호
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    • pp.634-648
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    • 2001
  • MSC는 통산 소프트웨어에서의 병행 수행 테스크들 사이의 상호작용을 기술하기 위해서 많이 사용되어져 왔다. 요구사항 단계에서 검증된 MSC 명세는 상태 기반의 설계 모델을 합성하는데 사용될 수 있을 뿐만 아니라, 테스크 시퀀스 생성에 사용될 수도 있다. 지금까지는 MSC에 기술된 각 이벤트의 위치 정보만을 이용하여 전체 상태 그래프를 생성함으로써 검증을 수행하였다. 본 논문에서는 MSC의 조건문을 시나리오 활성화 조건과 상태 변경을 기술할 수 있도록 확장하고, 이를 이용하여 전체 상태 그래프를 생성함으로써 테스크 시퀀스 생성에 이용한다. 생성된 전체 상태 그래프인 GFSM은 시스템의 의미 있는 상태 정보와 가능한 메시지 시퀀스만을 가지게 된다. 생성된 GFSM에 기존인 FSM 기반의 테스팅 기법을 적용하여 테스크 시퀀스를 추출할 수 있다.

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Finishing and polishing effects of multiblade burs on the surface texture of 5 resin composites: microhardness and roughness testing

  • Ehrmann, Elodie;Medioni, Etienne;Brulat-Bouchard, Nathalie
    • Restorative Dentistry and Endodontics
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    • 제44권1호
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    • pp.1.1-1.12
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    • 2019
  • Objectives: The aim of this in vitro study was to test the effect of 2 finishing-polishing sequences (QB, combining a 12/15-fluted finishing bur and an EVO-Light polisher; QWB, adding a 30-fluted polishing bur after the 12/15-fluted finishing bur used in the QB sequence) on 5 nanotech-based resin composites (Filtek Z500, Ceram X Mono, Ceram X Duo, Tetric Evoceram, and Tetric Evoceram Bulk Fill) by comparing their final surface roughness and hardness values to those of a Mylar strip control group (MS). Materials and Methods: Twelve specimens of each nanocomposite were prepared in Teflon moulds. The surface of each resin composite was finished with QB (5 samples), QWB (5 samples), or MS (2 samples), and then evaluated (60 samples). Roughness was analysed with an optical profilometer, microhardness was tested with a Vickers indenter, and the surfaces were examined by optical and scanning electron microscopy. Data were analysed using the Kruskal-Wallis test (p < 0.05) followed by the Dunn test. Results: For the hardness and roughness of nanocomposite resin, the QWB sequence was significantly more effective than QB (p < 0.05). The Filtek Z500 showed significantly harder surfaces regardless of the finishing-polishing sequence (p < 0.05). Conclusions: QWB yielded the best values of surface roughness and hardness. The hardness and roughness of the 5 nanocomposites presented less significant differences when QWB was used.

ROBUST TEST BASED ON NONLINEAR REGRESSION QUANTILE ESTIMATORS

  • CHOI, SEUNG-HOE;KIM, KYUNG-JOONG;LEE, MYUNG-SOOK
    • 대한수학회논문집
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    • 제20권1호
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    • pp.145-159
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    • 2005
  • In this paper we consider the problem of testing statistical hypotheses for unknown parameters in nonlinear regression models and propose three asymptotically equivalent tests based on regression quantiles estimators, which are Wald test, Lagrange Multiplier test and Likelihood Ratio test. We also derive the asymptotic distributions of the three test statistics both under the null hypotheses and under a sequence of local alternatives and verify that the asymptotic relative efficiency of the proposed test statistics with classical test based on least squares depends on the error distributions of the regression models. We give some examples to illustrate that the test based on the regression quantiles estimators performs better than the test based on the least squares estimators of the least absolute deviation estimators when the disturbance has asymmetric and heavy-tailed distribution.

Cloning and Sequence Analysis of Wild Argali ISG15 cDNA

  • Sun, Yanming;Chen, Kaili;Shen, Wen;Cui, Rupeng;Lu, Haifu
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권4호
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    • pp.561-566
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    • 2014
  • The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by $Ni^{2+}$ chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.

간이용 엘리베이터 장치 개선에 관한 연구 (A Study on the Improvement of Easy Elevator Equipment)

  • 위성동;구활본
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 기술교육위원회 창립총회 및 학술대회 의료기기전시회
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    • pp.82-88
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    • 2001
  • Manufactured easy elevator can drives from the first floor to fifth floor as sequence control circuit in cause opening than existing equipment of experiment and practice, the structure of in the first implementation process are hand-worked control component with push-button, L/S and relay, it is structured a lamp to express that the door open and moving of cage by mechanical action of For/Rev motor-braker of which load. The second structure of implement process to control from the first floor to the fifth floor with the PLC elevator program can control by the sensor of hand-operated function of L/Sl~L/S5 in time that the S/Wl~S/W5 of PLC control panel operates to the For/Rev. The function of two kind process that an elevator is driven by PLC program and the sequence control relay circuit is a mechanical relay sequence control field and it is equipment apparatus of it to get appropriately the technology of For/Rev in that mechanical operating cause of a load using the PLC program. Also the wring circuit using a plug, dissembly. the circuit and the principle of component, and PLC program with the function test can be used the implementation field to the total technology theory about FA.

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인공발(Prosthetic Foot) 스프링용 유리섬유강화 적층재의 적층배향에 따른 층간분리거동 해석 (Analysis of Delamination Behavior on the Stacking Sequence of Prosthetic Foot Keel in Glass fiber Reinforced Laminates)

  • 송삼홍;김철웅
    • 대한기계학회논문집A
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    • 제27권4호
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    • pp.623-631
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    • 2003
  • It is considered that the application of advanced composite materials to the prostheses for the disables is important to improve their bio-mechanical performance. Particularly, energy storing foot prosthesis is mostly important to restore gait ability of the disables with low-extremity amputation since it could provide propulsion at terminal stance enhancing the disables ability to walk long distance even run and jump. Therefore, the energy storing spring of Prosthetic foot keel under cyclic bending moment use mainly of high strength glass fiber reinforced plastic. The main objective of this study was to evaluate the stacking sequence effect using the delamination growth rate(dA$_{D}$/dN) of energy storing spring in glass fiber reinforced plastic under cyclic bending moment. The test results indicated that the shape of delamination zone depends on stacking sequence in GFRP laminates. Delamination area(A$_{D}$) turns out that variable types with the contour increased non-linearly toward the damage zones.nes.

Evaluation of Modified Turbo Spin Echo Technique Compared with Double Inversion Recovery Technique in Acquisition of Black Blood Brain Vessel Image

  • Choi, Kwan-Woo;Lee, Ho-Beom;Na, Sa-Ra;Son, Soon-Yong
    • Journal of Magnetics
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    • 제21권1호
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    • pp.148-152
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    • 2016
  • The main goal was to evaluate effectiveness of a modified TSE sequence compared with DIR (double inversion recovery) sequence in acquisition of fast flow brain vessel images using signal void effect. 32 healthy volunteers (10 men and 22 women; mean age of 31 years; ranging between 28-43 years) who underwent black blood DIR sequence (group A) and the modified TSE sequence (group B) were enrolled in our study. Signal to Noise Ratio (SNR) and Contrast to Noise Ratio (CNR) of the internal carotid arteries' lumen were compared in T1 and T2 weighted images for both group A and B. The images obtained from group B showed lower SNR values in internal carotid artery than the group A in both of the T1 and T2 weighted images (11.49% and 13.66% respectively). While the CNR values were higher in the group B than the group A in both of the T1 and T2 weighted images (8.69% and 7.55 % respectively). The qualitative score of all categories were not significantly different between the two groups. Furthermore approximately 49% of the total scan time was reduced from group B. Our study is to shorten the scanning time and minimize the inconveniences of the patients in acquisition of the black blood images of brain by using the signal void effect in the modified TSE technique while keeping the diagnostic value of the test.

비디오 얼굴 식별 성능개선을 위한 다중 심층합성곱신경망 결합 구조 개발 (Development of Combined Architecture of Multiple Deep Convolutional Neural Networks for Improving Video Face Identification)

  • 김경태;최재영
    • 한국멀티미디어학회논문지
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    • 제22권6호
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    • pp.655-664
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    • 2019
  • In this paper, we propose a novel way of combining multiple deep convolutional neural network (DCNN) architectures which work well for accurate video face identification by adopting a serial combination of 3D and 2D DCNNs. The proposed method first divides an input video sequence (to be recognized) into a number of sub-video sequences. The resulting sub-video sequences are used as input to the 3D DCNN so as to obtain the class-confidence scores for a given input video sequence by considering both temporal and spatial face feature characteristics of input video sequence. The class-confidence scores obtained from corresponding sub-video sequences is combined by forming our proposed class-confidence matrix. The resulting class-confidence matrix is then used as an input for learning 2D DCNN learning which is serially linked to 3D DCNN. Finally, fine-tuned, serially combined DCNN framework is applied for recognizing the identity present in a given test video sequence. To verify the effectiveness of our proposed method, extensive and comparative experiments have been conducted to evaluate our method on COX face databases with their standard face identification protocols. Experimental results showed that our method can achieve better or comparable identification rate compared to other state-of-the-art video FR methods.