• Archives of Pharmacal Research
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• v.24 no.4
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• pp.300-306
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• 2001

A novel and two known bioactive mono-tetrahydrofuran (THF) annonaceous acetogenins, annomocherin (1), annonacin (2) and annomontacin (3), have been isolated from the fractionated ethanolic extracts of the seeds of Annona cherimolia, guided by the brine shrimp lethality test (BST). Their structures were elucidated on the basis of spectroscopic and chemical methods. All compounds have a relative stereochemistry of threo/trans/threo for the mono-THF ring with two flanking hydroxyls. Compound 1 has a double bond at C-23/ 24 of aliphatic chain. Compound 1 was isolated from natural sources for the first time, and was named annomocherin. Two known Compounds 2 and 3 which have never been isolated from this species before, were obtained. Compound 1 exhibited potent and selective cytotoxicities against the breast carcinoma (MCF-7) and kidney carcinoma (A-498) cell lines with 100 to 1,000 times the potency of adriamycin. In brine shrimp lethality test (BST), 1-3 exhibited cytotoxicity.

• Korean Journal of Veterinary Service
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• v.20 no.4
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• pp.359-370
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• 1997

This study was carried out to Investigate the biochemical characteristics, antibiotic susceptibility, serogroups and pili producibility test of enterotoxigenic Escherichia coli(ETEC) isolated from piglets with diarrhea in Kangwon province from March to October 1996. 1. Sixty eight E coli strains were isolated from 72 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. 2. The serogroups of 26 isolates were classified as 08 : K87 6(8.8%), O20 : K1O1 4(5.9%), O141 : K85 4(5.9%), 09 : K103 : P987 3(4.4%), O45 : K 2(2.9%) 0139 : K82 2(2.9%), O64 : $K^{-}$2(2.9%), O149 : K91 1(1.5%), O157 : K88ac 1(1.5%) and O115 : $K^{-}$1(1.5%), respectively. 3. In antibiotic susceptibility test, the isolates showed high susceptible to Ak, Eno, Na, Gm, Am and Km, whereas resistance to Tc, Sm and Cf. 4. Sixty one strains(89.7%) of 68 I coli Isolates were resistant to one or more drugs. The isolates resistant to 2 and 3 or more drugs were 60.3% and 19.1%, respectively. Amog the 16 multiple resistant patterns, Sm Tc(11.5% ), Cf Sm Tc(11.5% ), Cf Cp Sm Su Tc(9.8% ) and Cf Cp Sm Su Tc(8.2%) patterns were frequently observed. 5. MRHA of guinea pig erythrocytes was detected in 9 out of 26 OK serotype and 9 out of 42 unidentified serotypes. MRHA titers of serotypes showed from 16 to 32 in O141 : K85 and no titers in O139 : K82. 6. By the GM1 ganglioside ELISA, $\beta$-, $\alpha$-, and $\gamma$-hemolysin producing strains was detected as 36, 6, and 5 from heat labile enterotoxin(LT) of 47 ETEC, respectively. The distribution of LT toxin from 112 isolates was showed $\beta$- hemolysin, 2 isolates $\alpha$-hemolysin and 3 isolates $\gamma$-hemol-ysin from 26 OK serotypes.

• Industry Promotion Research
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• v.9 no.3
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• pp.77-85
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• 2024

In this study, the hydrothermal extract of Chrysanthemum indicum Flowers are analyzed for the identification of its functional material value. Various aromatic compounds were detected as a result of GC-MS analysis, and antioxidant analysis confirmed the high antioxidant activity of the extract. High antibacterial activity was confirmed in the TIME KILL TEST using the 70% ethanol extract, which showed the highest activity, and the inhibition of intracellular melanogenesis was confirmed to be 115.77 ± 6.67%, 112.42 ± 1.64%, and 67.12 ± 3.89% compared to the α-MSH treatment group at concentrations of 1%, 1.2%, and 1.5%, respectively, when the extract was treated with 1%, 1.2%, and 1.5%. Collagenage activity inhibition assay showed collagenase inhibition of 56.57 ± 2.28%, 58.64 ± 2.99 and 29.53 ± 1.03% when treated at concentrations of 1.5%, 3% and 5%. The cytotoxicity test of the extract showed no cytotoxicity at concentrations of 1.5%, 3% and 5%. Therefore, this study predicts that the hydrothermal extract of Chrysanthemum indicum Flowers will be useful as a functional cosmetic material.

• The Korean Journal of Pharmacology
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• v.11 no.2
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• pp.41-46
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• 1975

The isolated strips of tortoise intestine are evaluated as a test organ for bioassay of prostaglandin $E_1$. This preparation responded highly sensitively to $PGE_1$ and $PGE_2$ in picogram concentration range. The mean slope and the value of precision index among the doses of 0.1, 0.3 and 0.5ng/ml in final concentration were 37.7 and 0.143, respectively. And this was relatively insensitive to different prostaglandins; $E_1/E_2{\gtrsim}1$, $E_1/A_2{\sim}50$ and $E_1/F_{2\alpha}{\sim}100$, and showed the dual responses to 5-hydroxytryptamine and histamine; initial contraction followed by relaxation. The dose-ratio inducing the relative equal contraction height for $PGE_1$, acetylcholine, caerulein, angiotensin and barium chloride was 0.4 : 50 : 25 : 10 : 100 in this order. These results suggest that the intestinal strips of the tortoise are suitable for bioassay of prostaglandin $E_1$ and $E_2$ between the doses of 0.1 and 1.0 ng/ml level in the tissue extracts.

• Journal of Korean Ophthalmic Optics Society
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• v.5 no.2
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• pp.21-25
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• 2000

The old age may experienced the visual acuity decrease according to refraction abnormality and they have a inconvenient real life style on viewing condition. So that, This study can provide the accurate information on the treatment of visual acuity of a male old ages by test of eye refraction state. The test was performed the visual acuity test by the object and subject methods.and the subjects was the Korean male over 55 old age. The eye types were 0.8 % positive for emmetropia, 15.2 % for myopia, and 84 % for hyperopia, respectively. The abnormal refraction eyes were 1.3 % positive for simple myopic astigmatism, 13.9 % for compound myopic astigmatism. 3.4 % for simple hyperopia and 3.1 % for simple hyperopic astigmatism, 77.5 % for mixed astigmatism, respectively. The asix of astigmatisms were 62% positive for against-the-rule astigmatism, 6% for with-the rule astigmatism, and 32 % for oblique astigmatism, respectively. On the total myopic spherical power, the $-0.50{\leq}$spheric equivalent <-2.00 diopter was 84.3%, the $-2.00{\leq}$spherical equivalent <-6.00.diopter was 15.7%. On the total hyperopic spherical power, the $+0.50{\leq}$spheric equivalent <+2.00 diopter was 74.2%, the $+2.00{\leq}$spherical equivalent <+6.00 diopter was 24.8%, and anything over the +6.00 diopter was 1%. On the astigmatic power, the $0.5{\leq}$cylinder <1.00 diopter was 54.1%, the $1.00{\leq}$cylinder <2.00 diopter was 32.4%, and anything over the 2.00 cylinder diopter was 13.5%.

• Journal of the Korean Society of Clothing and Textiles
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• v.12 no.1 s.26
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• pp.53-68
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• 1988

The purpose of this study was to investigate the clothing buying behaviorial pattern and the differences of clothing buying behaviors by the demographic variables. For the measurement of clothing buying behavior, the researcher developed a questionnaire. This questionnaire was adjusted by preliminary test. Data were collected from 494 women dwelling in Seoul, Busan, Daegu. For statistical analysis, frequency, percentage, average, standard deviation, paired t-test, $X^2-test$, one way analysis of variance, Duncan's multiple range test were used. The results were as follows: 1. The $57\%$ of the subject group planned their clothing purchase in advance, ana their friends affected clothing buying behaviors more than other fashion information sources. The store in which the subject group purchased clothing most frequently was a department store. In clothing selection, hygienic factor and aesthetic factor were shown as important factors. 2, There were significant differences in clothing buying behaviors according to demographic variables.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.58 no.1
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• pp.72-78
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• 2009

We have developed a current range extension method to obtain the ratio error and phase displacement of a current transformer (CT) by using absolute evaluation method and two different CTs. The method was applied to CTs under test with the current ratios in the range of 5,000 A / 1 A - 20,000 A / 1 A. The ratio error and phase displacement of the CT under test obtained in this study are consistent with those measured at the national institute in Germany using the same CT under test within an expanded uncertainty (k = 2) in the overall current ratios.

• Journal of Sericultural and Entomological Science
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• v.6
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• pp.42-48
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• 1966

Throughout the studies the following experimental results were obtained and so are summarized here. 1) What caused tussah silkworms terrible disease broken ous all over the Korea in 1965, was examined to be “Inclusion body of virus” through microscope. 2) The examined inclusion bodies are easily stained as purple by seller's staining. 3) The proper speed of centrifugation for the purification of inclusion bodies is 1,000 r. p. m for 10 minutes. 4) It is possible, cleanly resulted, to attempt the “Rapid Agglatination Test ＆ Complement Fixation test” with autigen of inclusion bodies. 5) The Anti-polyhedra rabbit serum from antigen of the dilution of 2${\times}$10$\^$6//l$m\ell$ polyhedra made the Ropid Agglutination test possible even with dilution of 1/8 infected pupa blood(antigen).

• Proceedings of the Korea Concrete Institute Conference
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• 1997.10a
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• pp.679-684
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• 1997

In this study, compressive strengths of reinforced concrete compression members rehabilitated with C.F.L. were analyzed from the test. Test parameters are spacing, spliced length, and section area of rehabilitation material. Displacement, failure load were measured during test. The failure mode and ultimate load were analyzed from these measured data. Test result shows that closer spacing of C.F.L. is more effective. strengthening with 1-ply C.F.L. is more effective than that of specimen with 2-ply C.F.L. The compressive capacity of specimen spliced ($\pi$.D)/2 shows almost similar strength to that of non-spliced specimen. The ultimate load carrying capacity of specimen strengthened with C.F.L. is increased to 1.11~1.68 times of that of non-rehabilitation specimen.

• Journal of TMJ Balancing Medicine
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• v.3 no.1
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• pp.27-30
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• 2013

Diagnostic evaluation methods frequently adopted in Temporomandibular Balancing Medicine (TBM) are briefly reviewed as to their basic concepts and procedures. TBM diagnostic evaluation methods may be classified into direct and indirect ones. Indirect methods are to observe any change in the manifestations with regard to the (1) posture, (2) pain, (3) muscle strength, and (4) stretching. Direct methods are to evaluate through (1) cervical palpation test, (2) restricted cervical rotation test, and (3) lateral cervical tension test. These methods may provide clinicians valuable information on the current yinyang balance and potential therapeutic factors in relation with the temporomandibular joint, a meridian system, and the whole body.