• Title/Summary/Keyword: Test 1.1 & 2.1

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Studies on enzootic bovine leukosis I. The changes of antibodies against bovine leukemia virus and peripheral blood lymphocyte on Holstein cattle in a dairy farm (축우의 유행형 (지방병성) 백혈병에 관한 연구 I. 한 유우군의 우백혈병 바이러스 항체와 혈중 임파구의 변동에 관하여)

  • Kim, Chan-ju;Son, Jae-young;Ko, Ki-whan
    • Korean Journal of Veterinary Research
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    • v.30 no.3
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    • pp.333-341
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    • 1990
  • To investigate bovine leukosis virus (BLV) infection in the cattle rearing in a dairy farm where a case of bovine lymphosarcoma had been identified several years ago, the 196 Holstein cattle including newborn calves to 12 years of age were tested. The BLV antibody test and peripheral lymphocyte count for bovine leukosis were carried out by the immunodiffusion (ID) test and Bendixen's Kep. These tests were performed 2 to 4 times at the interval of 3 to 5 months. The observed results were as follows: 1. The positive rates of BLV-antibody in the 1st, the 2nd, the 3rd and the 4th tests were 23.3%, 28.1%, 49.0% and 55.7%, respectively. The conversion rates from negative to positive in the 2nd, the 3rd and the 4th tests were 8.9%, 41.4%, and 20.0%, respectively. Results showed that the highest conversion rate was observed at the 3rd test which was conducted after winter. The highest positive rate by ID test were observed in 4 year old cattle in the 1st and 2nd tests, and in 2 year old herd in the 3rd and 4th tests. 2. In hematological test by Bendixen's Key, the positive and suspicious rates in the 1st, the 2nd, the 3rd and the 4th tests were 5.8 and 7.8%, 8.3 and 6.6%, 8.7 and 10.1%, 10.8 and 19.6% respectively. Results showed that the positive and. suspicious rates increased in course of time. 3. 70 to 100% of the positive cattle in hematological test were positive for BLV-antibody test. All of 13 cattle with persistent lymphocytosis (PL) were also positive for BLV-antibody, indicating the high relationship between PL and BLV-antibody. 4. The number of total leukocytes and absolute lymphocytes in the BLV-antibody positive cattle appeared significantly higher than those of the negative cattle. The markedly increased cell counts were observed in the cattle over one year old. 5. The mean of total leukocytes and absolute lymphocytes in the negative cattle for BLV-antibody increased slightly after sero-conversion into positive. 6. In the clinical examinations during experimental periods, none of the 72 positive cattle for BLV-antibody showed any lesions for bovine leukosis.

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Mutagenicity studies of food and cosmetic dyes (1) (식용 및 외용색소의 유전독성에 관한 연구 (1))

  • 하광원;정해관;오혜영;허옥순;손수정;한의식;정성철;한순영;최선주
    • Journal of Food Hygiene and Safety
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    • v.8 no.3
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    • pp.171-179
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    • 1993
  • The mutagenicity of 22 food and cosmetic dyes had been evaluated. Two different short-term mutagenicity tests were used: (1) Salmonella typhimurium preincubation assay (Ames test) (2) Chromosome aberration test with cultured Chinese hamster lung (CHL) fibroblast cells. Orange No. 203 was mutagenic in Salmonella typhimurium with and without rat liver microsomal activation, and Red No. 204 was mutagenic in Salmonelhl typhimurium with rat liver microsomal activation. Red No. 104-1 and Red No. 215 showed slight increase of chromosomal aberration in CHL cells.

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A Study on the Maneuverabilities of the Training Ship M.S.HEUIMANGBONG (실습선 희망봉호의 조정성능에 관한 연구)

  • Park, Seok-Pil;Kim, Gi-Yun
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.34 no.1
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    • pp.62-66
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    • 1998
  • The maneuverabilities of the training ship M.S. HEUIMANGBONG were studied, based on the data obtained from her Z test. The results obtained were summerized as follows: 1. The maneuvering indices K' and T'of the M.S. HEUIMANGBONG were 0.542, 1.305 at 10$^{\circ}$Z Test and 0.433, 1.351 at 20$^{\circ}$Z test and 0.442, 1.388 at 30$^{\circ}$Z test respectively. The above calculated values K', T' showed that her maneuverabilities were more effective when her rudder was used to small angle than to large angle. 2. As the overshoot angles of the starbord side of the M.S. HEUIMANGBONG were larger than those of port side at 10$^{\circ}$, 20$^{\circ}$and 30$^{\circ}$Z test, her maneuverabilities in port turning were found to be higher than in starbord turning. 3. The running distance of a turn at her 10$^{\circ}$Z test was about 9.9 times her own length and she was considered to have a good maneuverabilities synthetically

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Determination of Short-term Bioconcentration Factor on Carbofuran in Carassius auratus (goldfish) (Carassius auratus(goldfish)를 이용한 Carbofuran의 단기간 생물농축계수의 측정)

  • 민경진;배영규;차춘근;박천만;강회양
    • Journal of Environmental Health Sciences
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    • v.22 no.4
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    • pp.25-32
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    • 1996
  • The Bioconcentration factor(BCF) is used as an important criterion in the risk assessment of environmental contaminants. Also it can be used as indicator of biomagnification of environmentally hazardous chemicals through food-chain as well as a tool for ranking the bioconcentration potential of the chemicals in the environment. This paper reports the measured BCF value on carbofuran in Carassius auratus(goldfish), under steady state, and examined corelation between the BCF value and the depuration rate constant. Carassius auratus(goldfish) was chosen as test organism and test periods were 1-day, 3-day and 5-day. Experimental concentrations were 0.05, 0.10 and 0.50 ppm. Carbofuran in fish tissue and in test water was extracted with n-hexane and acetonitril. GC-ECD was used to detect and quantitate carbofuran. The depuration rate of carbofuran from the whole body of goldfish is determined over the 24-h period after treatment. The obtained results were as follows: 1. It was possible to determine short term BCFs of carbofuran through relatively simple procedure in environmental concentrations. 2. $BCF_1$ of carbofuran in concentration of 0.05, 0.10 and 0.50 ppm were 1.66, 1.64 0.61, $BCF_3$ were 2.08, 2.14, 0.66 and $BCF_5$ were 2.21, 2.57, 0.86, respectively. 3. Carbofuran concentration in fish extract was increased as increasing test concentration and prolonging test period, but $BCF_s$ in concentration of 0.50 ppm was greately decreased. 4. Determined deputation rate constants of carbofuran in concentration of 0.05, 0.10, 0.50 ppm were 0.076, 0.082 and 0.089, respectively. 5. It is considered that great decrease of $BCF_s$ in concentration of 0.50 ppm is due to high water solubility and stability of carbofuran in testwater. 6. It is suggested that low BCF of carbofuran is due to its relatively high water solubility and depuration rate, compared to BPMC, carbaryl and chlorothalonil.

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The Effect of Massage Therapy and Exercise Therapy on Body Composition and Blood Cholesterol in the Obese women (마사지 요법과 운동요법이 비만여성의 신체조성과 혈중 콜레스테롤에 미치는 영향)

  • Lee, Mi-Shun;Han, Mi-Jung;Lee, Young-Shun
    • Journal of the Korean Society of Fashion and Beauty
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    • v.2 no.1 s.1
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    • pp.93-102
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    • 2004
  • The purpose of this study is to examine the effect of massage therapy and exercise therapy on body composition and blood cholesterol in the obese women. Sixteen college women were randomly assigned to aged $20.1{\pm}2.7$ years who had not exercised regurlarly, volunteered to participate in the present study as subjects. They completed a 12 weeks massage therapy and exercise therapy performed 3 times/week, for 60min/session. Massage put in exercise treatment effect of 12 weeks in BMI has shown a significant decrease those are before test $25.1{\pm}2.2$ to after test $23.7{\pm}3.2$(p<0.05). The waist measure/height has a difference by before test r=0.5 to after a test r=0.47, and waist measure/hips measure by before test r=0.8 to after test r=0.75. A total cholesterol(TC) average value has $187.6{\pm}36.9$ before test to $168{\pm}32.4$(p<0.05) after test, a significant difference. Also average value of LDLC has $114{\pm}33.8$ before test to $99.2{\pm}30.4$(p<0.05) that shows a difference, but HDLC was $52{\pm}7.5$ before test to $52.3{\pm}7.7$(p<0.05) after test which means a none difference. HDLC does not have any difference but total cholesterol and LDLC were significantly different. It was a short program of 3 times a week, 60 min/session but, a dance meditation exercise program with high strength self massage had given a big effect in consideration. Using these scientific studies data, wish a program related by obese could make an activation in a beauty part.

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Subjective Symptoms of Fatigue in Normal Pregnant women (정상 임부가 자각하는 피로)

  • 김신정
    • Journal of Korean Academy of Nursing
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    • v.29 no.2
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    • pp.248-257
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    • 1999
  • The purpose of this study was to identify basic data for the health fare of normal pregnant women. The number of subjects was 779 pregnant women who received prenatal care in two University hospitals. The data, which were collected from June to October, 1998. and used the questionnaire “Symptom Table on Fatigue Perception” designed by the Research Committee of the Industial Fatigue in Hygienic. Association of Japan Industry. The collected data were scored by use of means and standard deviations according to the subjective symptoms of fatigue and each item as an independent variable was analysed by t-test and ANOVA test. The results are as follows : 1) Degree of subjective fatigue showed as an average of 1.81. Fatigue as physical symptoms had the highest score with 2.09. followed by neuro-sensory symptoms, 1.69 and psychological symptoms had the lowest score 1.66. 2) With the respect to the general characteristics of the subjects. there were statiscally significant difference in experience of pregnancy(t=-2.286, p=.023). wanted pregnancy(t=-2.935, p=.004), parity(t=-2.429, p=.015), sleeping time(F=3.478, p=.031). and presence of other child(t=2.347, p=0.19).

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Effect of Genotoxicity or Carcinogenecity Chemicals on the ROS Production (유전독성, 발암성 화학물질이 ROS 생성에 미치는 영향)

  • Go, Seo-Youn;Sheen, Yhun-Yhong
    • Environmental Analysis Health and Toxicology
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    • v.23 no.1
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    • pp.23-32
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    • 2008
  • In the present study, ROS detection of L5178Y cells that were treated with twenty test compounds in order to find out hydrogen peroxide ($H_2O_2$) induction for genotoxicity and carcinogenic toxicity. Twenty test compounds were consist of four classes, such as genotoxic carcinogens, genotoxic noncarcinogens, nongenotoxic carcinogens, and nongenotoxic noncarcinogens. Genotoxic carcinogens are 1,2-dibromoethane, glycidol, melphalan, diethylstilbestrol and urethane. Genotoxic noncarcinogens are 8-hydroxyquinoline, emodin, acetonitrile and diallylphthalate, L-ascorbic acid. Nongenotoxic carcinogens are methyl carbamate, O-nitrotoluene, 1,4-dioxane, tetrachloroethylene and 2,3,7,8-tetrachlorodibenzo-p-dioxin. And nongenotoxic noncarcinogens are D-mannitol, 1,2-dichlorobenzene, caprolactam, bisphenol A and chlorpheniramine maleate.

Studies on the Adherence and Corrosion Resistance of Oxide Coated Materials ( 1 ) (산화물 피복강재의 밀착성과 내식성에 관한 연구 ( 1 ))

  • Lee, Jong-Rark;Lim, U-Joh
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.32 no.2
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    • pp.157-163
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    • 1996
  • To ceramic film, $SiO_2$ and $Al_2O_3$, coated on pure Fe and stainless steel(SUS41O) by RF magnetron sputtering, the adherence between mm and substarte was studied. The adherence index (${\chi}$) was determined by the measure of micro hardness test. Also, the corrosion resistance on oxide coatings was studied using electrochemical measurement. The main results obtained are as the following: 1) In the micro-hardness test, with $1{\mu}m$ thickness mm, it has only one the value of ${\chi}$. Above $2{\mu}$thickness fIlm, however, get another value of ${\chi}$as the cracks in fIlm. 2) The oxide fIlm adhere well on the mild materials such as pure steel than high intensity materials like stainless. 3) Alumina($Al_2O_3$) coated materials have better corrosion resistance than silica($SiO_2$)coated materials

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Eye Irritation Test of Lens Washing Agents SOLOCARETM on New Zealand White Rabbits (렌즈 보존액 SOLOCARETM에 대한 안점막자극성시험)

  • Jang, Woo-Yeong;Park, Eun-Kyu;Kim, Ki-Hong
    • Journal of Korean Ophthalmic Optics Society
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    • v.11 no.4
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    • pp.299-310
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    • 2006
  • The eye irritant test of lens washing agent, $SOLOCARE^{TM}$ was conducted using Draize methods according to KFDA Guidelines 1999-61. In addition, to test the potential toxicity of test articles the ratio of inflammatory cells and non-inflammatory epitheloid cells were also observed using smear cytology methods against ocular discharge. At sacrifice, the histopathological changes on Cornea, Iris, Retina and Sclera were also observed in all animals. Slight (1~2 degrees) irritancy of cornea and conjunctiva were observed at 1, 2 and 3 days after dropping in non-washing group. The MIOI of these points are detected as 4.33, 3.33 and 2.00, respectively. In washing group, slight irritancy of cornea and conjunctiva were restricted to 1 and 2 days after dropping with MIOI as 3.00 and 1.33, respectively. Therefore, $SOLOCARE^{TM}$ was also considered as non-irritating materials because the MIOI is detected below 5.00 throughout the whole experimental periods in both washing and non-washing groups and the IAOI is also detected as 4.33 (1 day after dropping). No meaningful changes on smear cytology of ocular discharges are observed in this study compared to that of non-treated intact eyes. In addition, no abnormal histopathological changes on the cornea, iris, retina and sclera were also detected in $SOLOCARE^{TM}$ dropping group compared to that of non-treated intact eyes.

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Tooth whitening effect of toothpaste containing hydrogen peroxide (과산화수소가 배합된 제제의 치아미백효과에 관한 연구)

  • Ahn, Jae-Hyun;Kim, Ji-Hye;Kim, Jong-Hoon;Jang, Jong-Hwa;Oh, Yoon-Jong;Park, Yong-Duk
    • Journal of Korean society of Dental Hygiene
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    • v.14 no.1
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    • pp.101-108
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    • 2014
  • Objectives : The purpose of this study was to evaluate the tooth whitening effect of 0.74% and 2.80% hydrogen peroxide toothpastes and safety on tooth and gingival tissue. Methods : Toothpastes containing 0.74% and 2.80% hydrogen peroxide were evaluated. In in-vitro test, some additives (sodium metaphosphate, sodium pyrophosphate and titanium dioxide) were added to the toothpastes. Hydroxyapatite specimens (HAPs) were made and stained using modified Stookey's methods. HAPs were treated for 1 hour at shaking incubator and brushed for 1,000 times as 250 gF with each diluted toothpaste. Before and after color was measured by colorimeter. Using double blind method, 99 Korean with natural maxillary anterior teeth were selected and the initial brightness (baseline) was measured by SHADEEYE-EX. Based on this measurement they were crossly distributed into control group (0% hydrogen peroxide), test 1 (0.74% hydrogen peroxide) and test 2 (2.80% hydrogen peroxide). After 2 weeks, people of each group were provided toothpaste and told to use 3 times a day right after every meal for 3 minutes. The brightness of teeth was measured 3 times for every one month. Results : ${\Delta}L$ was statistically significant among three groups in shaking test. ${\Delta}L$ of two test groups was statistically significant compared with control group but not between each test group in brushing test. After using toothpaste for 3 months, test 1 group and test 2 group were 15.89% and 31.23% more whitened compared with control group respectively (p<0.05). Rate of more whitened person of each test group was 24.2% and 40.5% more than control group respectively (p<0.05). There was no difference in the hypersensitivity during 3 months using toothpastes and no side effect on teeth or gums. Conclusions : Toothpastes containing 0.74% and 2.80% hydrogen peroxide showed tooth whitening effect and both were safe enough to use for tooth whitening.