• Journal of the Korean Chemical Society
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• v.42 no.2
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• pp.209-213
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• 1998

The higher order structure of Pseudomonas alcaligenes 5S rRNA has been investigated by using ($\eta^{6}$-mesitylene) manganese (Ⅰ) tricarbonyl hexafluorophosphate[MTH-Mn (Ⅰ)], dimethylpyrocarbonate, potassium permanganate as chemical probes. The sequences cleaved strongly by MTH-Mn (Ⅰ) on the tertiary structure of the 5S rRNA are $G_{12}AUGG_{16}$ of loop a, $G_{51}AAGUGAAGC_{60}$ of the region b-C, $U_{65}-AGCG_{69}$. of the region B-a, and $G_{72}AUGG_{76}$ of loop d. Based on such cleavage patterns of 5S rRNA by MTH-Mn(Ⅰ) and other chemical probes, we presume that the sequences strongly cleaved form pocket-like structure as in the the corner of L structure of $tRNA^{Phe}$. We also presume that the region b-C and loop d together play a role of hinge in forming the pocket-like structure in the 5S rRNA.

• Journal of Life Science
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• v.12 no.4
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• pp.464-468
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• 2002

Enzymatic activities and fluorescence spectroscopic properties of the double mutant proteins P96L/F139W, P96L/F258W and a triple mutant protein P96L/F139W/F258W of tryptophan synthase $\alpha$ subunit from Escherichia coli was examined to study tertiary and local structure changes around the tryptophan residues. The enzymatic activities of P96l./F139W and P96L/F258W were similar, but P96L/F139W/F258W had lower activity, as compared to wild type. The fluorescence intensities of double mutant, P96L/F139W and P96L/F258W, were decreased but that of a triple mutant, P96L/F139W/F258W, was increased when compared to wild type. The sum of the maximum fluorescence intensity (fluorescence intensity at the λ$_{max}$) for the double mutant proteins was not equal to the intensity seen in the triple mutant protein. The enzymatic activity and fluorescence data indicate that the replacement of Pro$^{96}$ longrightarrowLeu might affect on the stability of helix 8 and the loop located between strand 4 and helix4. The result suggests that the tertiary structure of triple mutant (P96L/F139W/F258W), being different from wild type, might have more compact residual structure at the vicinity of 139 and 258.8.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.27 no.5
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• pp.235-242
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• 2017

In order to prepare porous scaffolds capable of pore control, PMMA powder serving as a pore-forming agent was added to HA powder to synthesize a slurry containing TBA as a solvent. And then, porous HA scaffolds where pillarshaped pore channels interconnected with each other were fabricated by freeze-casting and sintering. The crystal structure of the HA scaffolds according to the addition amount of PMMA powder was measured by XRD and the surface and inner cross section of the scaffolds were analyzed through SEM. It was found that removal of PMMA during sintering affects the internal structure of the scaffolds and the crystallinity of the HA powder. Furthermore, through evaluating the physical and mechanical properties of the scaffolds, it was confirmed that the porosity, pore size and compressive strength can be controlled by controlling the addition amount of the pore-forming agent. It was also found that the HA scaffolds produced in this study were similar in structure and properties to the natural cancellous bone. This suggests that porous HA scaffolds with PMMA can be used as an alternative to autogenous bone for tissue engineering as an artificial bone scaffold.

• Journal of the Korean Solar Energy Society
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• v.39 no.4
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• pp.1-9
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• 2019

Modules using 6 inch cells have problems with loss due to empty space between cells. To solve this problem made by shingled structure which can generate more power by utilizing empty space by increasing the voltage level than modules made in 6inch cell. Thus, in this paper, the c-Si cutting cells were produced using nanosecond green laser, and then the ECA was sprayed and cured to perform cutting cell bonding. Three types of ECA materials (B1, B2, B3) with Ag as the main component were used, and experimental conditions varied from 5 to 120 seconds of curing time, 130 to $210^{\circ}C$ of curing temperature, and 1 to 3 of curing numbers. As a results of experiments varying curing time, B1 showed efficiency 19.88% in condition of 60 seconds, B2 showed efficiency 20.15% in 90 seconds, and B3 showed efficiency 20.27% in 60 seconds. In addition, experiments with varying curing temperature, It was confirmed highest efficiency that 20.04% in condition of $170^{\circ}C$ with B1, 20.15% in condition of $150^{\circ}C$ with B2, 20.27% in condition of $150^{\circ}C$ with B3. These are because the Ag particles are densely formed on the surface to make the conduction path. After optimizing the conditions of temperature and curing time, the secondary-tertiary curing experiments were carried out. as the structural analysis, conditions of secondary-tertiary curing showed cracks that due to damp heat aging. As a result, it was found that the ECA B3 had the highest efficiency of 20.27% in condition of 60 seconds of curing time, $150^{\circ}C$ of curing temperature, and single number of curing, and that it was suitable for the manufacture of Solar cell of shingled structure rather than ECA B1 and B2 materials.

• Korean Journal of Occupational Health Nursing
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• v.27 no.3
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• pp.180-189
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• 2018

Purpose: The aim of this study was to investigate the influence of nurses' awareness of healthcare accreditation on their job stress and turnover intention. Methods: Across-sectional correlation study design was used. Participants consisted of 143 nurses from two tertiary hospitals in Seoul and Gyunggido, South Korea. Data were collected in October 2016 using self-report questionnaires and analyzed using descriptive statistics, t-test, an ANOVA, Pearson's correlation coefficient, and multiple regression in SPSS/WIN 21.0. Results: Awareness of health care accreditation correlated negatively with job stress (r=-.63, p<.001) and turn over intention (r=-.50, p<.001). Awareness of health care accreditation was the factor that most influenced job stress, explaining 38% of the variance (F=23.10, p<.001). Awareness of health care accreditation and duty pattern were the factors that most influenced intention to leave, explaining 32% of the variance (F=10.35, p<.001). Conclusion: These findings suggest raising nurses' awareness of certification systems to reduce their job stress and turnover intentions. It will be necessary to provide support for the aggressive work nurses do and improve their work structure, highlighting the need for both manpower and institutional support. Accordingly, providing regular education programs and appropriate compensation schemes, by raising nurses' awareness of medical institutions' certification systems, is necessary.

• Economic and Environmental Geology
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• v.9 no.1
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• pp.45-73
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• 1976

This study has been made for the enlargement of a previous work of 1964 which was carried out by an author of this work emphasizing the stratigraphy, micropaleontology, depositional environment, and structural tectonics of the studied area. The stratigraphic sequences of the area are groupped into four units: (1) basement of Pre-Cretaceous, (2) lower sediments of Late Cretaceous, (3) upper sediments of Late Cretaceous and (4) igneous rocks of Late Cretaceous and Tertiary (?). The oldest rocks consisting of schists and gneisses of Pre-Cambrian and schistose granite' of Jurassic age are exposed at the base of this area on which the thick Cretaceous sediments were deposited. These old rocks are unconformably overlain by the lower sedimens of Late Cretaceous composed of three members, an alternation of black shale and tuffaceous sediments, fine tuff and rhyollite flow in ascending order. The oily material was found from the black shales of the alternation m"ember as semi-solid greaselike material, oily order and microscopic granular spherical material and oily stain. The lower sediments are also overlain, in low-angleunconfromity, by the 'upper sediments having three members, an alternation of volcanic conglomerate and andesitic tuff, rhyollitic tuff and andesite flow in the same order. The igneous suit of diabase, diorites, biotite granite, porphyritic granite and porphyries of the latest Cretaceous and small exposure of pitchstone of Tertiary (?) intruded into the pre-existed rocks above mentioned. Considerable amount of ostra- coda microfossils have been chemically extracted from the black shales of the lower sediments and the identification of the fossils suggests that the depositional environment of the sediments were under fresh or brackish water condition. The distribution of the geology and its tectonic data also suggest a combination of dome and basin structures in the area of San-i peninsula and Jin-do as shown in fig. 8. Between these two units an anticlinal structure was constructed. As a result of this study, a seismic survey in a district between U-su-yong and north coast of Jin-do is recommended to determine the underground features.

• Journal of Korean Academy of Nursing Administration
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• v.3 no.2
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• pp.109-122
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• 1997

This Study was dosigned to test the relationship between effectiveness of nursing organization and related variables of organization on the perspectives of total quality management, The data were collected through self reported questionnaires from 138 nurses working in, 288 patients hospitalized in one tertiary hospital in Seoul from May 30 to October 10 in 1996. The data were analyzed by using the pc-SAS program. The information was obtained of descriptive statistics, correlation analysis, multiple regression, ANOVA & Tukey's multiple comparison test. Results showed that the managing change, the managing job design, the managing conflict, the formation and the centralization of nursing organization structure were shown to be relating variables for nurses' job satisfaction. Especially the managing change was the predictor of nurses' job satisfaction, It explained 57% of total variance. According to clustor analyses of the variables of the managing change, the managing job design, the managing conflict, the nursing units were divided three clusters and according to ANOVA, there was significint difference in nurses' job satisfaction The nurses' job satisfaction was higher in the nursing units revealed to have high job management score. But there was no significant difference in patient satisfaction among three clusters.

• Journal of The Korea Institute of Healthcare Architecture
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• v.23 no.4
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• pp.37-53
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• 2017

Purpose: As the structure of the disease has been changed and the infectious disease has been increased, the demand for diagnostic examination has been increasing. So, the department of laboratory medicine in hospital has playing the important role accounting for about nineteen percent of total medical expenses in korea. This study is to investigate the laboratory function and spatial composition of the department and figure out the space area and space organization. Methods: Explore the literature review to identify the laboratory function. Limit to five cases of tertiary level general hospital having about a thousand bed and analyze the space layout and floor area to confirm the spatial composition. Classify the exam function and check the spatial composition and spatial organization. Results: This study allows 5 conclusions to be summarized. Laboratory medicine divided into nine part in regulation, but in space allocated into core lab, emergency lab and six part lab. Total laboratory area is $2,036m^2$ in average, and is composited with 60% for lab, 17% for office and 20% for public. Lab area per a bed presents $1.88m^2$. Microbiolgy and molecular lab area are getting large. Laboratory space organized into the four zone, like an entrance zone, core zone, rear zone and peripheral zone. Emergency and transfusion lab are allocated in entrance zone, hematology chemistry and immunology in core lab, microbiology and molecular lab in rear, support offices in peripheral zone. The most important point was to check the spatial composition of the Laboratory Medicine according to the inspection function. Implications: This study can be used as a useful data in planning and designing a Laboratory Medicine Department.

• Journal of Microbiology and Biotechnology
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• v.29 no.9
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• pp.1460-1469
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• 2019

The extensive distribution of multidrug-resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) poses a threat to healthcare worldwide. This study aimed to investigate the MDR and molecular patterns of MRSA isolates in children admitted to the two biggest tertiary care pediatric hospitals in northern and southern Vietnam. A total of 168 MRSA strains were collected to determine antibiotic susceptibility by minimum inhibitory concentration tests. Antibiotic-resistant genes, pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing were used for the molecular characterization of MRSA. Among the total strains, the MDR rate (51.8%) was significantly higher in the northern hospital than in the southern hospital (73% vs. 39%, p < 0.0001). The MDR-MRSA with the highest rates were "ciprofloxacin-erythromycin-gentamicintetracyclines" (35.6%), followed by "erythromycin-tetracycline-chloramphenicol" (24.1%), and "ciprofloxacin-erythromycin-gentamicin" (19.5%), showing an accumulative total of 79.3%. The most susceptible antibiotics were rifampicin (100%) and vancomycin (100%), followed by doxycycline (94.0%), meropenem (78.0%), and cefotaxime (75.0%). The SCCmecII strains showed greater resistance to gentamicin, ciprofloxacin, tetracycline, meropenem and cephalosporins compared with the other strains. The SCCmecII strains exhibited the highest rate in the tested genes (aacA/aphD: 55.2%, ermA/B/C: 89.7%, and tetK/M: 82.8%). ST5-SCCmecII was the predominant clone in the northern hospital, whereas SCCmecIVa was more pronounced in the southern hospital. In conclusion, our results raised concerns about the predominant MDR-MRSA strains in the pediatric hospitals in Vietnam. The north-south difference in the antibiotic resistance patterns and genetic structure of MRSA suggests different MRSA origins and various uses of antimicrobial agents between the two regions.

• Bulletin of the Korean Chemical Society
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• v.13 no.2
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• pp.199-207
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• 1992

The approximate rates and stoichiometry of the reaction of excess sodium diethyldihydroaluminate (SDDA) with 68 selected organic compounds containing representative functional groups were examined under standard conditions (THF-toluene, $0^{\circ}C$ in order to compare its reducing characteristics with lithium aluminum hydride (LAH), aluminum hydride, and diisobutylaluminum hydride (DIBAH) previously examined, and enlarge the scope of its applicability as a reducing agent. Alcohols, phenol, thiols and amines evolve hydrogen rapidly and quantitatively. Aldehydes and ketones of diverse structure are reduced rapidly to the corresponding alcohols. Reduction of norcamphor gives 11% exo-and 89% endo-norborneol. Conjugated aldehydes such as cinnamaldehyde are rapidly and cleanly reduced to the corresponding allylic alcohols. p-Benzoquinone is mainly reduced to hydroquinone. Hexanoic acid and benzoic acid liberate hydrogen rapidly and quantitatively, however reduction proceeds very slowly. Acid chlorides and esters tested are all reduced rapidly to the corresponding alcohols. However cyclic acid anhydrides such as succinic anhydride are reduced to the lactone stage rapidly, but very slowly thereafter. Although alkyl chlorides are reduced very slowly alkyl bromides, alkyl iodides and epoxides are reduced rapidly with an uptake of 1 equiv of hydride. Styrene oxide is reduced to give 1-phenylethanol quantitatively. Primary amides are reduced very slowly; however, tertiary amides take up 1 equiv of hydride rapidly. Tertiary amides could be reduced to the corresponding aldehydes in very good yield ( > 90%) by reacting with equimolar SDDA at room temperature. Hexanenitrile is reduced moderately accompanying 0.6 equiv of hydrogen evolution, however the reduction of benzonitrile proceeds rapidly to the imine stage and very slowly thereafter. Benzonitrile was reduced to give 90% yield of benzaldehyde by reaction with 1.1 equiv of hydride. Nitro compounds, azobenzene and azoxybenzene are reduced moderately at $0^{\circ}C$, but nitrobenzene is rapidly reduced to hydrazobenzene stage at room temperature. Cyclohexanone oxime is reduced to the hydroxylamine stage in 12 h and no further reaction is apparent. Pyridine is reduced sluggishly at $0^{\circ}C$, but moderately at room temperature to 1,2-dihydropyridine stage in 6 h; however further reaction is very slow. Disulfides and sulfoxides are reduced rapidly, whereas sulfide, sulfone, sulfonic acid and sulfonate are inert under these reaction conditions.