• 생명과학회지
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• 제10권3호
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• pp.279-285
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• 2000

cAMP 수용성 단백질인 CRP는 Escherichia coli에서 대사와 관련된 유전자의 전사를 조절한다. 본 연구는 야생형과 돌연변이 CRP 단백질의 열적 안정성과 온도에 따른 단백질의 구조변화를 관찰하기 위 하여 proteolytic digestion, UV spectrophotometer, CD spectrapolarimeter 등의 방법을 사용하였다. cAMP가 없을 때에는 야생형, S83G, S128A CRP가 열적 안정성에서 큰 차이를 보이지 않았지만, cAMP가 존재할 때 야생형 CRP가 다른 돌연변이 CRP보다 열적으로 더욱 안정함을 보였다. 그리고 protease digestion 실험을 통하여 높은 온도에서 cAMP의 존재와 무관하게 돌연변이 CRP에서 단백질 의 변성으로 인한 절단된 단백질띠를 관찰할 수 있었다. 그리고 55$^{\circ}C$에서 측정한 CD 스펙트럼에서 단백 질의 2차 구조인 $\alpha$-helix 구조가 부분적으로 파괴되었음이 관찰되었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권4호
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• pp.274-278
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• 2001

Polymer membranes composed of N, N-dimethylaminoethyl methaccrylate(DMAEMA) and acrylamide(AAm)(or ethyl acrylamide(EAAm) were prepared to demonstrated the thermo-control of solute permeation. Poly DMEMA has a lower critical solution temperature(LCST) at around 50$\^{C}$ in water, With the copolymerization of DMAEMA with AAm (or EAAm) a shift in the LCST to a lowere temperature was observed, probably due to the formation of hydrogen bonds between the amide and N-N-dimethylamino groups. However, the temperature-induced phase transition of poly(DMAEMA-co-EAAm) did not show a similar trend to that of poly(DMAEMA- co-AAm) in the gel state. The hydrogen bonds in poly(DMAEMA-co-EAAm) were significantly disrupted with the formation a gel network, which led to a difference in the swilling behavior of polymer gels in response to temperature. To apply these polymers to temperature-sensitive sol-ute permeation, polymer membranes were prepared. The permeation pattern of hydrocortisone, used as the model solute, was explained based on the temperature-sensitive swelling behavior of the polymer membranes.

• Journal of the korean society of oceanography
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• 제38권3호
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• pp.122-134
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• 2003

Low-pass filtered time series of wind, coastal temperature, sea level and current were analyzed to understand the coastal upwelling processes in the southeast coast of Korea. Southerly winds favorable for coastal upwelling were dominant in summer of 1997. Total period of four major wind events amounts to 58 days during one hundred days from June to early September. Coastal temperature is most sensitive to variations of wind. The time lag between the onset of southerly (northerly) winds and decrease (increase) of temperature is 3-18 hours. In the frequency domain the coherent bands have periods of 2.4 and 4.0-5.4 days with respective phase lags of 17 and 27-37 hours. Despite the sensitive response, the magnitude of temperature change is not quantitatively proportional to the intensity or duration of the wind, because it depends on the degree of baroclinic tilting of isotherms built dynamically by the strong Tsushima Warm Current (TWC). Current is particularly strong near the coast and has a large vertical shear during the upwelling periods, which is associated with the baroclinic tilting. Both of current and sea level are poorly coherent with wind or temperature except for the period of 4 days.

• 한국농림기상학회지
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• 제3권1호
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• pp.55-70
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• 2001

It was unusual crop weather for 1998 and 1999 compared with normal in Korea. The consecutive days of the optimum ripening period for rice plant that had daily mean temperature 21~23$^{\circ}C$ for 40 days after flowering, increased with long anomalies in 1998~99. The air temperature during ripening period was much higher than the optimum temperature and lower sunshine hour than norm in the local adaptability tests of newly developed rice lines during those years. In response of rice cultivation to warming and cloudy weather during crop season, the yield shall be decreased. Most scientists agree that the rate of heating is accelerating and temperature change could become increasingly disruptive. Weather patterns should also become more erratic. Agrometeorologists could be analyzed yearly variations of temperature, sunshine hour and rainfall pattern focused on transient agroclimate change for last a decade. Rice agronomists could be established taking advantage of real time agricultural meteorology information system for fertilization, irrigation, pest control and harvest. Also they could be analyzed the characteristics of flowering response of the recommended and newly bred rice cultivars for suitable cropping plan such as cultural patterns and sowing or transplanting date. Rice breeders should be deeply considered introducing the characteristics of basic vegetative type of flowering response like Togil rices as prospective rice cultivars corresponding to global warming because of the rices needed higher temperature at ripening stage than japonica rices, photoperiod-sensitive and thermo-sensitive ecotypes.

• 폴리머
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• 제36권2호
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• pp.223-228
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• 2012

온도와 pH 민감성을 갖는 그래프트 공중합체[Pluronic-$g$-poly(NIPAAm-$co$-MMA), Polymer A]와 [Pluronic-$g$-poly(NIPAAm-$co$-MAA), Polymer C]는 $t$-butylperoxybenzoate를 이용하여 Pluronic 공중합체의 주사슬에 $N$-isopropylacrylamide (NIPAAm)/$N,N$-diethylaminoethylmethacrylate (DEAEMA)와 $N$-isopropylacrylamide(NIPAAm)/methacrylic acid (MAA)를 각각 합성하였다. 그래프트 공중합체는 $^1H$ NMR과 젤 투과 크로마토그래피를 통해 공중합체의 화학적 구조와 분자량을 측정하였다. 그래프트 공중합체의 수용액 상에서의 특성은 다른 온도와 pH 조건에서 자외 및 가시선 분광 분석법, 접촉각 측정과 동적 광산란으로 측정되었다. 그래프트 공중합체는 수용액상에서 온도와 pH에 따라 민감한 상 변화를 보인다. 이는 DEAEMA 단량체의 아민 그룹과 MAA 단량체의 카복실 그룹은 각각 Polymer A와 Polymer C에서 하한임계용액온도에 큰 영향을 미친다고 제시한다. 그래프트 공중합체는 온도와 pH 변화에 관련된 다양한 약물 전달과 분자 스위치 적용에 사용될 수 있다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3098-3103
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• 2007

In this paper, natural convection of a magnetic fluids(W-40) in a half circular pipe enclosure are investigated by numerical and experimental method. One side wall is kept at a constant temperature(25$^{\circ}C$), and the opposite side wall is also kept at a constant temperature(20$^{\circ}C$). Under above conditions, various magnitudes of the magnetic fields were applied up. Theoretical study through the governing equation derived by Siliomis is carried out with numerical analysis by the GSMAC Method. And the thermo-sensitive liquid crystal film(R20C5A) is utilized in order to visualize wall-temperature distributions as an experimental method. This study has resulted in the following fact that the natural convection of a magnetic fluids are controlled by the direction and intensity of the magnetic fields.

• 분석과학
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• 제30권4호
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• pp.213-219
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• 2017

Polyalkylsiloxane has been spotlighted in pressure-sensitive adhesive (PSA) application due to excellent physical properties and good biocompatibility. Thermal behaviour of polyalkylsiloxane mixtures, such as thermal stability and heat flow, were studied using TG-DTA during catalytic hydrosilation. To understand reaction kinetics of cross-linking, catalytic hydrosilation of polyalkylsiloxane was monitored using variable temperature nuclear magnetic resonance (VT-NMR) as increased temperature. The formation of cross-linking bond $Si-CH_2-CH_2-Si$ was directly observed using distortionless enhanced by polarization transfer (DEPT) technique. Successfully polyalkylsiloxane PSA samples exhibited excellent adhesion properties by cross-linking reaction.

• 한국미생물·생명공학회지
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• 제17권1호
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• pp.81-83
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• 1989

The flagella antigenic variation of nine Bacillus thuringiensis serovar kurstaki temperature-sensitive mutants grown at the permissive temperature (3$0^{\circ}C$) was detected by a serological agglutination between H-antigen and antiserum. The flagella antigens were injected to rabbits to prepared their antisera, and then their homologous and heterologous titers of the antisera were measured. The homologous titers were ranged from 1:6,400 to 1:12,800, but the heterologous titers were very low. The H-antigen of the wild type strain was not agglutinated to 4 heterologous antisera, ts-U23 not to 7, ts-U3l not 5, ts-U32 not to 4, ts-U33 not to 7, ts-U7l not to 4, ts-U73 not to 6, ts-U74 not to 6, ts-U91 not to 4 and ts-U603 not to 4 antisera.

• Journal of Advanced Marine Engineering and Technology
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• 제33권7호
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• pp.987-993
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• 2009

An experimental study is performed to investigate unsteady mixed convection in a horizontal channel with a heat source. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualization and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. It is found that the periodic flow of mixed convection in a cavity appears at very low Reynolds numbers (Re<0.4), and the period decreases with increasing Reynolds numbers and increases with increasing aspect ratio.

• Structural Engineering and Mechanics
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• 제16권2호
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• pp.115-126
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• 2003

A novel finite element model based on the incremental endochronic theory with the effect of temperature was developed in this study to explore the deformed behaviors of a flexible pavement material. Three mesh systems and two loading steps were used in the calculation process for a specimen of three-dimensional circular cylinder. Computational results in the case of an uni-axial compression test for temperatures at $20^{\circ}C$ and at $40^{\circ}C$ were compared with available experimental measurements to verify the ability of developing numerical scheme. The isotropic response and the deviatoric response due to the thermal effect were presented from deformations in different profiles and displacement plots for the entire specimen. The characteristics of changing asphalt concrete material under a specified loading condition might be seen clearly from the numerical results, and might provide an useful information in the field of road engineering.