• BMB Reports
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• 제43권7호
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• pp.451-460
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• 2010

Starting with the first publication of lacZ gene fusion in 1980, reporter genes have just entered their fourth decade. Initial studies relied on the simple fusion of a promoter or gene with a particular reporter gene of interest. Such constructs were then used to determine the promoter activity under specific conditions or within a given cell or organ. Although this protocol was, and still is, very effective, current research shows a paradigm shift has occurred in the use of reporter systems. With the advent of innovative cloning and synthetic biology techniques and microfluidic/nanodroplet systems, reporter genes and their proteins are now finding themselves used in increasingly intricate and novel applications. For example, researchers have used fluorescent proteins to study biofilm formation and discovered that microchannels develop within the biofilm. Furthermore, there has recently been a "fusion" of art and science; through the construction of genetic circuits and regulatory systems, researchers are using bacteria to "paint" pictures based upon external stimuli. As such, this review will discuss the past and current trends in reporter gene applications as well as some exciting potential applications and models that are being developed based upon these remarkable proteins.

• 한국환경과학회지
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• 제29권12호
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• pp.1171-1184
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• 2020

The green synthesis of inorganic nanoparticles (NPs) using biomaterials has garnered considerable attention in recent years because of its eco-friendly, non-toxic, simple, and low-cost nature. In this study, we synthesized NPs of noble metals, such as Ag and Au using an aqueous extract of a marine seaweed, Ecklonia cava. The formation of AgNPs and AuNPs was confirmed by the presence of surface plasmon resonance peaks in UV-Vis absorption spectra at approximately 430 and 530 nm, respectively. Various properties of the NPs were evaluated using characterization techniques, such as dynamic light scattering, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. Phytochemicals in the seaweed extract, such as phlorotannins, acted as both reducing and stabilizing agents for the growth of the NPs. The green-synthesized AgNPs and AuNPs were found to exhibit high catalytic activity for the decomposition of organic dyes, including azo dyes, methylene blue, rhodamine B, and methyl orange.

• 한국생산제조학회지
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• 제20권5호
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• pp.564-569
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• 2011

The current establishment of city gas piping polyethylene (PE) tube used as bonding state or part of the health or safety of fusion is very important. A part of these fusion methods to determine the soundness of the short-term trials and long-term tests can be largely classified. Typical tests include short-term strength, tensile strength, impact strength, compressive strength, resiliency and compression. Polyethylene (PE) pipes installed in the domestic terms of overall penetration rate of 45% has been used. However, polyethylene (PE) pipes have reliability problems, and these occurs mostly in part by defective welding. Therefore, the test is necessary for safety. Non-destructive methods (ultrasonic testing) are difficult to be used. Therefore, Polypropylene copolymer (PP-C), polypropylene homopolymer (PP-H), and polyethylene (PE) pipe are used. Fusion of these materials is necessary in these field however, its technical, and basic research has not been studied well. In this research, short-term strength of welding parts, its tensile strength, hardness, fatigue, and microstructure have been analyzed to find the optimum process conditions to improve mechanical properties.

• 한국측량학회지
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• 제39권5호
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• pp.289-295
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• 2021

Recently, owing to the development of autonomous vehicles, research on precisely determining the position of a moving object has been actively conducted. Previous research mainly used the fusion of GNSS/IMU (Global Positioning System / Inertial Navigation System) and sensors attached to the vehicle through a Kalman filter. However, in recent years, new technologies have been used to determine the location of a moving object owing to the improvement in computing power and the advent of deep learning. Various techniques using RNN (Recurrent Neural Network), LSTM (Long Short-Term Memory), and NARX (Nonlinear Auto-Regressive eXogenous model) exist for such learning-based positioning methods. The purpose of this study is to compare the precision of existing filter-based sensor fusion technology and the NARX-based method in case of GNSS signal blockages using simulation data. When the filter-based sensor integration technology was used, an average horizontal position error of 112.8 m occurred during 60 seconds of GNSS signal outages. The same experiment was performed 100 times using the NARX. Among them, an improvement in precision was confirmed in approximately 20% of the experimental results. The horizontal position accuracy was 22.65 m, which was confirmed to be better than that of the filter-based fusion technique.

• Nuclear Engineering and Technology
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• 제52권5호
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• pp.1002-1007
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• 2020

The purpose of the current study was to evaluate a spectrum formulation set employed to modify the neutron spectrum of D-D fusion neutrons in a IS plasma focus device using GEANT4, MCNPX2.6, and FLUKA codes. The set consists of a moderator, reflector, collimator and filters of fast neutron and gamma radiation, which placed on the path of 2.45 MeV neutron energy. The treated neutrons eliminate cancerous tissue with minimal damage to other healthy tissue in a method called neutron therapy. The system optimized for a total neutron yield of 10⁹ (n/s). The numerical results indicate that the GEANT4 code for the cubic geometry in the Beam Shaping Assembly 3 (BSA3) is the best choice for the energy of epithermal neutrons.

• Food Science and Biotechnology
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• 제18권2호
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• pp.500-505
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• 2009

The fibrinolytic enzyme, subtilisin D5, was purified from the culture supernatant of the isolated Bacillus amyloliquefaciens DJ-5. The molecular weight of subtilisin D5 was estimated to be 30 kDa. Subtilisin D5 was optimally active at pH 10.0 and $45^{\circ}C$. Subtilisin D5 had high degrading activity for the A$\alpha$-chain of human fibrinogen and hydrolyzed the $B{\beta}$-chain slowly, but did not affect the $\gamma$-chain, indicating that it is an $\alpha$-fibrinogenase. Subtilisin D5 was completely inhibited by phenylmethylsulfonyl fluoride, indicating that it belongs to the serine protease. The specific activity (F/C, fibrinolytic/caseinolytic activity) of subtilisin D5 was 2.37 and 3.52 times higher than those of subtilisin BPN' and Carlsberg, respectively. Subtilisin D5 exhibited high specificity for Meo-Suc-Arg-Pro-Tyr-pNA (S-2586), a synthetic chromogenic substrate for chymotrypsin. The first 15 amino acid residues of the N-terminal sequence of subtilisin D5 are AQSVPYGISQIKAPA; this sequence is identical to that of subtilisin NAT and subtilisin E.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.1464-1466
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• 2003

classification of the satellite images provides information about land cover and/or land use. Quality of the classification result depends mainly on the spatial and spectral resolutions of the images. In this study, image fusion in terms of resolution merging, and band integration with multi-source of the satellite images; Landsat ETM+ and Ikonos were carried out to improve classification. Resolution merging and band integration could generate imagery of high resolution with more spectral bands. Precise image co-registration is required to remove geometric distortion between different sources of images. Combination of unsupervised and supervised classification of the fused imagery was implemented to improve classification. 3D display of the results was possible by combining DEM with the classification result so that interpretability could be improved.

• 환경영향평가
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• 제20권1호
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• pp.49-59
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• 2011

The results of comparing $PM_{10}$ concentration between 'Namsan' and 'Yongsan-gu' air quality monitoring stations show similar values with averaged concentration in the whole Seoul. The correlation factors in both sites were 0.865, 0.828 in 2005, 2006, respectively. For 'Bukhansan' and 'Gangbuk-gu' air quality monitoring stations, different from the results mentioned above, they showed clear differences as altitude changes. PM10 concentration in 'Bukhansan' monitoring stations was 10 ${\mu}g/m^3$ lower than 'Gangbuk-gu' monitoring station which is located near the ground. Also, averaged PM10 concentration in 'Bukhansan' and 'Gangbuk-gu' monitoring stations was lower than that in the whole Seoul. When comparing $NO_2$ concentration between 'Namsan' and 'Yongsan-gu' monitoring stations, $NO_2$ concentration in 'Namsan' monitoring station was lower than 'Yongsan-gu' monitoring station. For $NO_2$ concentration in 'Bukhansan', 'Gangbuk-gu' and 'the whole Seoul', there were the same pattern in 'Gangbuk-gu' and the 'the whole Seoul' and low values in 'Bukhansan' monitoring station. The correlation factors of $NO_2$ concentration in 'Bukhansan' and 'Gangbukgu' was 0.525, 0.549 in 2005, 2006, respectively, which stands for low correlationship.

• 폴리머
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• 제34권6호
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• pp.547-552
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• 2010

생분해성 고분자는 제약 및 생명공학 분야에서 많은 관심을 받고 있는 물질로 특히 나노미립구의 형태로 약물전달체의 개발에 널리 이용되고 있다. 본 연구에서는 cyclohexanedimethanol과 oxalyl chloride를 pyridine의 존재 하에서 반응하여 고분자량의 생분해성 퍼옥살레이트 고분자를 합성하고 그 물리화학적 및 생물학적 특성을 조사하였다. 폴리옥살레이트는 분자량이 약 75000 Da 정도인 반결정성 고분자이며 물에서 가수분해가 일어남을 GPC와 NMR로 확인하였다. 소수성의 폴리옥살레이트는 단일유화법으로 나노미립구로 제조될 수 있으며 약물을 포접할 수 있고 아주 우수한 세포안정성을 가졌다. 용이한 합성과 우수한 물리화학적 및 생물학적 특성을 바탕으로 폴리옥살레이트 나노미립구는 약물전달체 개발에 아주 높은 잠재력이 있음을 확인하였다.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2007년도 동계학술대회
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• pp.60-63
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• 2007

최근, 핸드폰은 일상생활의 필수품이 되었다. 특히 카메라가 장착된 핸드폰은 개인홍보와 오락요소로의 대표적인 도구로 사용된다. 나아가 양안카메라가 적용된 핸드폰은 사용자에게 입체감을 전달할 것이며, 입체감은 보다 효과적인 개인홍보 및 오락성을 제공할 것이다. 핸드폰은 휴대가 용이하지만 제한된 표시소자의 크기 및 해상도로 인하여 입체시 구현 시 사용자에게 손실된 정보 및 낮은 임장감을 제공한다. 이에 본 연구는 기존의 입체시 구현에 대한 구속조건들을 분석하고 핵심 요인을 산출하여, 핸드폰 환경에서의 최적의 입체시 구현을 위한 새로운 구속조건의 범위를 정량화하였다. 대표적인 구속조건으로는 수평, 수직 시차량이 고려되었다. 본 연구의 결과는 핸드폰용 양안카메라의 제작공차 및 효과적인 시차영상 제작을 위한 기준을 제시한다. 연구에 사용된 제품으로는 QVGA해상도(320*240) 및 2.8' 크기의 표시소자인 PDA폰이며, 입체시에 대한 구속조건을 정량화하기 위하여 수직, 수평이동이 가능한 지그를 사용하여 실험의 정확성을 높였다. 피실험자는 10명이며 남자 7명, 여자 3명으로 구성되었다.