• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1115-1123
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• 2016

_L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the _L-asparaginase gene (_L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of _L-ASPG86 (_L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The _L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant _L-asparaginase (r-_L-ASPG86) showed optimum conditions at 37-40℃, pH 9. Moreover, r-_L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-_L-ASPG86 was 687.1 units/mg under optimum conditions (37℃, pH 9, and 5 mM MnSO₄).

• Journal of The Korean Association of Information Education
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• v.23 no.5
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• pp.491-497
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• 2019

As the world develops into a global society, more and more people want to speak foreign languages fluently. To speak fluently, you must have sufficient training in speaking, which requires a dialogue partner. Recently, it is expected that the development of voice recognition information technology will enable the development of a system for conducting foreign language speaking training without human beings from the other party. In this study, a test bed system for foreign language speaking training was developed and applied to elementary school classes. Elementary school students were asked to present their English conversation situation and conduct speaking training. Then, satisfaction with the system and potential for continuous utilization were surveyed. The system developed in this study has been identified as helpful for the training of learning to speak a foreign language.

• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2711-2719
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• 2013

Hydrogen bonding interaction between alcohols and water molecules is an important characteristic in the aqueous solutions of alcohols. In this paper, a series of molecular dynamics simulations have been performed to investigate the aqueous solutions of low molecular weight alcohols (methanol, ethylene glycol and glycerol) at the concentrations covering a broad range from 1 to 90 mol %. The work focuses on studying the effect of the alcohols molecules on the hydrogen bonding of water molecules in binary mixtures. By analyzing the hydrogen bonding ability of the hydroxyl (-OH) groups for the three alcohols, it is found that the hydroxyl group of methanol prefers to form more hydrogen bonds than that of ethylene glycol and glycerol due to the intra-and intermolecular effects. It is also shown that concentration has significant effect on the ability of alcohol molecule to hydrogen bond water molecules. Understanding the hydrogen bonding characteristics of the aqueous solutions is helpful to reveal the cryoprotective mechanisms of methanol, ethylene glycol and glycerol in aqueous solutions.

• Computers and Concrete
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• v.26 no.2
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• pp.161-173
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• 2020

Shear keys in precast concrete segmental bridges (PCSBs) are usually match-casting which is very labour intensive. In this research, an innovative match-casting-free construction was proposed by leaving small gap between the convex and the concave castellated shear keys in the joints of PCSBs. Specimen experiment, shear strength analysis and numerical simulation were conducted, investigating the loading performance of this new type of dry joints, the gap dry joints. Compared with match-casting joint specimens, it has been found from experiment that shear capacity of gap joint specimens significantly decreased ranging from 17.75% to 42.43% due to only partially constrained and contacted in case of gap dry joints. Through numerical simulation, the effects of bottom contacting location, the heights of the gap and the shear key base were analyzed to investigate strength reduction and methods to enhance shear capacity of gap joint specimens. Numerical results proved that shear capacity of gap dry joints under full contact condition was higher than that under partial contact. In addition, left contact destroyed the integrity of shear keys, resulting in significant strength reduction. Larger shear key base remarkably increased shear capacity of the gap joint. Experimental tests indicated that AASHTO provision underestimated shear capacity of the match-casting dry joint specimens, while the numerical results for the gap dry joint showed that AASHTO provision underestimated shear capacity of full contact specimens, but overestimated that of left contact specimens.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.05a
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• pp.709-711
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• 2018

Although the supply of smartphones by the silver generation has increased in recent years, low learning, experience levels and understanding make it difficult to use them. In thid paper, we proposes remote control UI applications to support the use of smartphones by the silver generation. For the proposed application, the key features of the feature phone are arranged in a $3{\times}2$ arrangement on the Home screen to enable quick recognition and proposed application provides remote control via Node.js server and socket communication, in case of difficulties in the use of incidental function, increases the understanding and availability levels of the silver generation using smart phones by providing support through remote control.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.3
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• pp.339-345
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• 2001

In this paper, FG-CPW (Finite-Ground Coplanar Wave-Guide) balanced diode mixer is presented. Frequency bandwidth is selected for a C-band, which is 5.72~5.82 GHz for RF, 5.58~5.68 GHz for LO, and 140 MHz for IF signals. A rat-race hybrid is designed for the accomplishment of single balanced type. A low pass filter (LPF) with CPW structure is used far good conversion loss and unwanted harmonics suppression. When LO signal with the power of 4 dBm at 5.635 GHz is injected, a conversion loss of 6.2 dB is obtained for the mixer. Also, the LO to RF and LO to IF isolation of 30 dB and 40 dB are obtained, respectively. This mixer can be used in the area on wireless LAN application.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.05a
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• pp.453-455
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• 2022

Metadata is data that explains attributes and features to the data as structured data. Among them, video metadata refers to data extracted from information constituting the video for accurate content-based search. Recently, as the number of users using video content increases, the number of OTT providers is also increasing, and the role of metadata is becoming more important for OTT providers to recommend a large amount of video content to individual users or to search appropriately. In this paper, a study was conducted on a method of automatically extracting metadata for mood attributes through sound effects of images. In order to classify the sound effect of the video and generate metadata about the attributes of the mood, I would like to propose a method of establishing a terminology dictionary for the mood and extracting information through supervised learning.

• Annual Conference of KIPS
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• 2017.04a
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• pp.113-116
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• 2017

최근 급격히 증가한 모바일 기기와 Over The Top (OTT) 서비스의 활성화로 인하여 CMM 기반의 LTE/EPC 네트워크에 과다한 데이터/제어 트래픽의 수용이 힘들어지는 문제가 중요 이슈로 부각되고 있다. 이를 해결하기 위하여 IETF는 Distributed Mobility Management (DMM) 기반의 이동성 관리 방안을 제안하였다. 하지만 DMM 기술은 중앙의 트래픽 부하 분산에 초점을 두고 있어 단말의 이동과 관련하여 발생하는 과다한 제어 트래픽 수용에 관한 문제를 해결하기에는 부족하다. 따라서 본 논문에서는 이러한 문제를 해결하기 위하여 SDN 기반으로 CMM과 DMM을 함께 이용하는 HMM (Hybrid Mobility Management) LTE/ECP 네트워크 모델을 제시한다. 또한 HMM 기반의 LTE/EPC 네트워크 모델은 CMM 및 DMM 기법의 선택을 위해 단말의 이동성과 PDN 연결의 개수를 고려한 선택방안을 제시하며, 제안하는 HMM 기반의 LTE/EPC 네트워크 구조에서의 데이터 트래픽 부하량과 단말의 이동성과 PDN 연결 개수에 따라 제어 트래픽의 양을 비교하는 그래프를 제시하며 제안하는 네트워크 모델의 타당성을 입증한다.

• Proceedings of the KAIS Fall Conference
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• 2009.12a
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• pp.842-845
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• 2009

본 연구에서는 신 에너지전원(태양광)이 배전계통에 연계되어 운용되는 것을 상정하여, 정상상태와 비상(사고)상태, 전력품질(순시), 스마트배전설비 등에 대한 배전계통의 특성 및 신 에너지전원의 특성을 시험하고 분석할 수 있는 분산전원용 계통연계 시뮬레이터를 제작하고 평가하기 위하여, 배전계통 전용해석 S/W인 PSCAD/EMTDC를 이용하여 사전 시뮬레이션을 수행하여 설계를 수행하고, 시험결과를 분석하였다. 본 연구에서 제작한 시뮬레이터는 배전용변전소(154/22.9KV)의 주변압기와 3상 4선식 22.9KV 고압배전선로의 2개 선로 및 부하를 축약형태(약 1/100)로 모의한 것이지만, 주상변압기(13,200/380/220V) 이하의 저압 배전선로는 실계통과 거의 동일한 상태로 모의한 것이다. 이상의 구성요소 데이터를 산출하기 위하여 PSCAD/EMTDC를 이용하여 사전 시뮬레이션을 수행하여 설계 및 제작을 수행하고 그 효용성을 확인하였다.

• Communications of the Korean Mathematical Society
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• v.37 no.1
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• pp.31-43
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• 2022

In his notebooks, Srinivasa Ramanujan recorded several modular equations that are useful in the computation of class invariants, continued fractions and the values of theta functions. In this paper, we prove some new modular equations of signature 2 by well-known and useful theta function identities of composite degrees. Further, as an application of this, we evaluate theta function identities.