• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.761-768
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• 2007

Serratia proteamaculans HY-3 isolated from the digestive tract of a spider produces an extracellular protease named arazyme, with an estimated molecular mass of 51.5 kDa. The purified enzyme was characterized as having high activities at wide pH and temperature ranges. We further characterized biochemical features of the enzymatic reactions under various reaction conditions. The protease efficiently hydrolyzed a broad range of protein substrates including albumin, keratin, and collagen. The dependence of enzymatic activities on the presence of metal ions such as calcium and zinc indicated that the enzyme is a metalloprotease, together with the previous observation that the proteolytic activity of the enzyme was not inhibited by aspartate, cysteine, or serine protease inhibitors, but strongly inhibited by 1,10-phenanthroline and EDTA. The araA gene encoding the exoprotease was isolated as a 5.6 kb BamHI fragment after PCR amplification using degenerate primers and subsequent Southern hybridization. The nucleotide sequence revealed that the deduced amino acid sequences shared extensive similarity with those of the serralysin family of metalloproteases from other enteric bacteria. A gene(inh) encoding a putative protease inhibitor was also identified immediately adjacent to the araA structural gene.

• Smart Media Journal
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• v.9 no.4
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• pp.91-96
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• 2020

A blockchain is a technology in which all nodes participating in a distributed network manage each transaction's contents without a central server managing the transaction, which is a record of the transaction. The block containing the transaction record of a specific period is connected to the blockchain by referring to the hash value for the previous block, and the chain with the new block added is shared with all nodes again. Transactions using existing certificates will pass through FinTech, and in the near future, applications using blockchains are expected to emerge. In this study, we analyze the problems of the existing model, and propose a transaction model that applies the blockchain to come. Among various applications, this study develops a trading model targeting the energy sales market among the topics that will lead the fourth industrial revolution. As a result of analyzing the proposed model, it was possible to be sure of the possibility of a safer energy sales transaction than the existing method.

• The Transactions of the Korean Institute of Power Electronics
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• v.27 no.1
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• pp.26-32
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• 2022

This paper proposes a power system that includes a 120k W fuel cell DC-DC converter (FDC) and 30 kW bidirectional DC-DC converter (BHDC) for a 150 kW fuel-cell vehicle. With a high DC link voltage of 800 V, the efficiency and power density of the power electronic components are improved. Through the modular design of FDC and BHDC, electric components are shared, resulting in reduced mass production costs. The switching frequency of 30 kHz of full SiC devices and optimal design of coupled inductor reduce the volume, achieving a power density of 8.3 kW/L. Furthermore, a synergetic operation strategy using variable limiter control of FDC and BHDC was proposed to efficiently operate the fuel cell vehicle considering the fuel cell stack efficiency according to the load. Finally, the performance of the prototype was verified by Highway Fuel Economy Driving Schedule testing, EMI test, and the linked operation between FDC and BHDC. The full load efficiencies of the FDC and BHDC prototypes are 98.47% and 98.74%, respectively.

• Journal of IKEEE
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• v.5 no.1 s.8
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• pp.100-110
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• 2001

In this paper, we investigated several buffer management schemes for the design of shared-memory type ATM switches, which can enhance the utilization of switch resources and can support quality-of-service (QoS) functionalities. Our results show that dynamic threshold (DT) scheme demonstrate a moderate degree of robustness close to pushout(PO) scheme, which is known to be impractical in the perspective of hardware implementation, under various traffic conditions such as traffic loads, burstyness of incoming traffic, and load non-uniformity across output ports. Next, we considered buffer management strategies to support QoS functions, which utilize parameter values obtained via connection admission control (CAC) procedures to set tile threshold values. Through simulations, we showed that the buffer management schemes adopted behave well in the sense that they can protect regulated traffic from unregulated cell traffic in allocating buffer space. In particular, it was observed that dynamic partitioning is superior in terms of QoS support than virtual partitioning.