• Bulletin of the Korean Chemical Society
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• 제33권9호
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• pp.2907-2909
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• 2012

A disintegrin and metalloprotease with thrombospondin domains (ADAMTS) are a member of peptidase and involved in processing of von Willebrand factor as well as cleavage of aggrecan, versican, brevican and neurocan. Among 19 subfamilies of human ADAMTS, ADAMTS-4 is a zinc-binding metalloprotease and is a famous therapeutic target for arthritis. It has been reported that a flavonoid luteolin shows inhibitory activity against ADMATS-4. In this study, we verified that luteolin is a potent inhibitor of ADAMTS-4 and probed the molecular basis of its action. On the basis of a docking study, we proposed a binding model between luteolin and ADAMTS-4 in which 3',4'-dihydroxyl groups in luteolin formed hydrogen bonding with ADMATS-4 and these interactions are important for its inhibitory activity against ADAMTS-4.

• Toxicological Research
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• 제31권4호
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• pp.393-402
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• 2015

This study was conducted to measure toxicity of 1-chloropropane (CAS No. : 540-54-5). According to the OECD Test Guideline 413 (Subchronic inhalation toxicity: 90-day study), SD rats were exposed to 0, 310, 1,250, and 5,000 ppm of 1-chloropropane for 6 h/day, 5 day/week for 13 weeks via whole-body inhalation. Mortality, clinical signs, body weights, food consumption, motor activity, ophthalmoscopy, hematology, serum chemistry, urinalysis, organ weights, gross and histopathological findings were compared between control and all tested groups. No mortality or remarkable clinical signs were examined during the study. No gross lesions or adverse effects on body weight, food consumption, motor activity, ophthalmoscopy, urinalysis, hematology, organ weights were observed in any of male or female rats in all tested groups. In serum biochemistry, glucose was significantly decreased in males of 1,250 and 5,000 ppm groups compared to control group in dose-dependent relationship. In histopathological examination, vacuolation of acinar cells was observed in pancreas of all male and female groups exposed to 1-chloropropane. In conclusion, no observable adverse effect level (NOAEL) was considered to be below 310 ppm/6 h/day, 5 day/week for rats.

• Preventive Nutrition and Food Science
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• 제8권4호
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• pp.349-355
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• 2003

Effects of extraction conditions, gamma-irradiation and storage conditions on bioactivities of Citrus (Citrus unshiu) peel extract were investigated. The Hunter color $L^{*}$- and $a^{*}$-values of the extract increased but $b^{*}$-value decreased with an increase in absorbed irradiation dose. DPPH radical scavenging, tyrosinase inhibition and nitrite scavenging activities were not affected by irradiation but reduced by increased storage time. Nitrite scavenging activity of the extract was the highest at pH 1.2 followed by pH 4.2 and 6.0 and not changed by storage. Results indicated that there is potential for using citrus peel byproducts as a bioactive ingredient, and that gamma irradiation brightens the color of the extract without adversely altering its biological activity.ity.

• 한국균학회지
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• 제51권4호
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• pp.265-276
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• 2023

본 연구는 담수 환경에서 분리한 곰팡이의 특성을 알아보기 위해 효소 활성을 평가하였다. 40개의 곰팡이들은 다양한 담수 시료로부터 분리되었으며, 계통분석을 통해 동정하였다. 실험에 사용된 균주들은 최근에 국내에 보고되었거나, 아직 보고되지 않은 종으로서 이에 대한 특성 정보가 거의 알려지지 않았다. 본연구에서는 40개 균주를 대상으로 protease, amylase, lipase, cellulase, laccase, chitinase의 효소에 대해서 활성을 검정하였다. 대부분의 균주가 laccase 활성을 보였으며, protease, amylase 순으로 높게 나타났다. 담수 환경에서의 효소 활성 정보는 이들의 생태적 역할을 이해하고 산업적으로 활용하는데 기여할 수 있을 것이다.

• KSBB Journal
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• 제21권5호
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• pp.376-383
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• 2006

솔잎착즙액(PNE)은 엽록소 함유량(40.8 mg/100 g)이 높고 비타민 C도 다량함유(149 mg/100 g)하고 있어 기능성 식품으로 개발가능성이 높으며, 솔잎 발효액은 향미가 좋아지고 기능성이 높아져서 고부가가치가 크므로 발효의 조건과 활성도를 조사 분석하여 고기능성 솔잎발효액(SFPE)을 개발하고자 하였다. 솔잎자체발효(SFPE)에 관여하는 균을 발효단계에 따라 분리, 선발하고 동정하였다. GPYA배지에서 발효균을 스크린하여 Pichia galeiformis, Candida boidinii, Pichia species, Candida species1, Candida species2, Candida ooidensis, Saccharomyces cerevisiae, Candida karawaiewii를 선발하였으며, 발효가 진행됨에 따라 2년차에는 Candida boidinii, Candida species2, Candida ooidensis, Saccharomyces cerevisiae가 선발되었고, 3년차에는 Candida species1&2가 선발되어 Candida species2가 우점종으로 나타났다. 그러나 발효 4년차에는 어떤 균도 선발되지 않았다. Candida species2는 Candida ernobii와 99.65%의 homology를 보였다. E. coli, Bacillus subtilis와 Staphylococcus aureus에 대해서 SFPE를 농도별로 처리하였을 때 높은 항균활성을 확인하였다. SFPE 1, 4, 7의 항산화 활성은 $0.025{\mu}{\ell}/ml$의 농도로 처리하였을 경우 SFPE 1, 4, 7이 약 34.8%의 NBT scavenging의 활성을 보여주었고, $0.2{\mu}{\ell}/ml{\sim}0.3{\mu}{\ell}/ml$의 농도에서는 SFPE 1, 4, 7이 약 90%에 달하는 NBT scavenging 의 활성능력을 확인할 수 있었다. 또한 전기생리학적 실험을 통하여 솔잎발효액이 쥐의 혈관수축 이완에 미치는 효과를 분석한 결과 Phenylephrine(PE)($10^6M$)으로 유발된 수축반응에 대해 SFPE를 0.15 mg/ml과 0.3 mg/ml을 첨가하였을 때 혈관이 다시 이완되었으며, ATP 민감성 $K^+$ 채널 억제제인 glibenclamide($10^5M$)을 처리하였을 때 SFPE 7 효과가 나타나지 않은 것으로 보아 SFPE의 혈관 이완작용이 세포막 ATP-민감성 $K^+$ 통로를 활성화시켜 이루어짐을 확인하였다.

• 원예과학기술지
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• 제31권6호
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• pp.813-820
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• 2013

울릉도 자생 삼나물(Aruncus dioicus)은 최근 monoterpenoids 생리활성 물질이 밝혀지면서 여러 가지 항산화와 관련된 생리활성이 확인되고 있다. 삼나물 ethyl acetate 분획물에 의한 미백효과를 검증하기 위하여 흑색세포종인 B16F10을 이용하여 실험을 진행하였다. 삼나물 ethyl acetate 분획물(ADE)의 세포독성을 측정하기 위하여 MTT assay를 실시한 결과, ADE $500{\mu}g{\cdot}mL^{-1}$ 이상의 농도에서 미비한 세포독성(10% 이상)을 확인하였으며, 이후 실험에서는 5, 10, $50{\mu}g{\cdot}mL^{-1}$ 농도가 세포 내에서 tyrosinase 활성과 멜라닌 양의 변화를 측정하였다. 그 결과 ADE 농도에 따라 tyrosinase 활성이 감소하고 총 멜라닌 함량이 감소하는 것을 확인하였다. 특히 $50{\mu}g{\cdot}mL^{-1}$에서 35.6% tyrosinase 활성억제, 58.8% 멜라닌 함량이 감소하는 것을 확인하였다. 또한 ADE에 의해 미백과 관련된 tyrosinase, tyrosinase related protein 1(TRP1), TRP2, microphthalmia associated transcription factor(MITF) 및 그 상위 단계인 cAMP와 protein kinase A(PKA)의 단백질 양이 감소하여 cAMP response binding protein(CREB)의 인산화는 감소하고 extracellular signal related kinase(ERK)의 인산화는 증가하는 것을 확인하였다. 본 연구에서는 울릉도 자생 삼나물의 미백효과에 관한 효능을 확인하고 기능성화장품의 소재로서의 활용 가능성이 있음을 확인하였다.

• 한국식품영양과학회지
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• 제34권6호
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• pp.797-804
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• 2005

폐자원을 이용한 기능성 신소재 개발 연구를 위해 감귤 음료 가공 중 생성되는 부산물인 감귤 과피를 추출·정제하여 얻어진 감귤정유의 생리활성 효과를 검증하고 감마선에 의한 영향도 확인하였다 감귤 정유의 limonene의 함량은 $88.3\pm1.30\%$로 나타났으며 감마선 조사한 시료에서도 limonene의 함량은 차이가 없는 것으로 나타났다. 감귤 정유의 전자공여능은 약 70$\%$로 나타났으며 돈육에 감귤 정유 첨가 시 지질산패가 억제 되는것을 확인할 수 있었다. 감글 정유에 대한 항균 활성은 paper disc diffusion test 결과를 바탕으로 농도에 따른 생육저해효과를 측정한 결과 yeast는 250 ppm에서, bacteria는 $2,500\~5,000$ ppm에서, mold는 $5,000\~10,000$ ppm에서 생육이 억제되어, yeast에 대한 항균활성이 가장 우수한 것으로 나타났다. MTT assay를 이용한 세포독성실험 결과 대부분의 암세포에서 약 $90\%$ 이상의 억제율을 보였으며 Ames test를 이용한 복귀돌연변이 시험에서는 $200\mu g/plate$이 하일 때 감귤 정유의 돌연변이 원성이 나타나지 않는 것으로 확인되었다. 전체적인 시험 결과 감마선 조사에 의한 생리활성 변화의 차이는 없는 것으로 나타났다. 따라서 식품 및 공중보건제품 소재로서 가공부산물인 귤 정유는 경제적이면서 효과적으로 사용될 수 있으리라 기대된다.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.568-571
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• 2000

Cepacidine A was previously isolated as a novel antifungal antibiotic from the culture broth of Pseudomonas cepacia AF2001. It exhibits a potent in vitro antifungal activity against various plant pathogenic fungi, such as Plasmopora veticola on grapes, Septoria nodorum and Fusarium culmorum on wheat, as well as Colletotrichum lagenarium on cucumbers. Accordingly, this study was conducted to evaluate the potential crop protection activity of strain P. cepacia AF2001. The strain was tested in semi-greenhouse biocontrol assays, and showed an excellent biological activity against Pythium ultimum in cotton and cucumbers; however, only a minor activity against Rhizoctonia aolani in cotton was observed. Furthermore, the nematodes Haemonchus contortus and Trichostrongylus only exhibited a moderated activity in the in vitro larval development assay with no activity in the in vivo animal model.

• International Journal of Advanced Culture Technology
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• 제9권3호
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• pp.334-344
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• 2021

The study has suggested a way to minimize safety accidents found in on-site firefighting activity by analyzing firefighters' [investigation report on accidents to on-sie workers]. The study result is described as follows. First, the result of [investigation report on on-site workers] shows that while accidents are found to most frequently have occurred to low-ranking firefighting officers such as Sobangsa and Sobanggyo and firefighting officers with career of less than 5 years, fire-extinguishing activity proved to be the most prone to accidents in the category of activity. Second, analysis performed to identify variance between injury extent and area based on cause of accident shows statistically significant variance. In addition, the result of verifying difference in cause of accident based on category of activity shows significant difference with 'falling over and sliding' being the highly likely cause of accident in fire containment and rescue activities and 'reckless move' being highly likely case of accident in emergency activities. Third, the result of verifying factors behind the extent of injury done to on-site workers shows that when accident is caused by 'incomplete behavior', it was found that the extent of injury is substantial. It was also found that rescue activity is accompanied by substantial extent of injury. As a solution to this, the study suggested ways to establish, extend and operate safety-specific curriculum for entrants, develop materials regarding risk prognosis training and explicate training-related regulations, set up safety management measure for a single squad team, upgrade performance of private protection equipment, institutionalize SOP by on-site activity stage, materialize and activate swift rescue team and increase objectivity and proficiency of safety accident investigation.

• Bulletin of the Korean Chemical Society
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• 제31권4호
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• pp.915-920
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• 2010

A novel 2',4'-dimethyl carbocyclic adenosine 5'-phosphonic acid analogue (20) was prepared using acyclic stereoselective route from commercially available 4-hydroxybutan-2-one (4). To improve cellular permeability and enhance the anti-HCV activity of this phosphonic acid, a 3',5'-cyclic SATE phosphonodiester nucleoside prodrug (22) was prepared. The synthesized phosphonic nucleoside analogues, (20) and (22), were assayed for their ability to inhibit HCV RNA replication in a subgenomic replicon Huh7 cell line.