• Journal of the Korean Wood Science and Technology
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• v.15 no.2
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• pp.79-89
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• 1987

This study was executed to examine helical structure and other feature, of Taxus cuspidata S. et Z. and Larix gmelinii var. principis-ruprechtii Pilger through photomicroscopic and scanning electron microscopic observation, and the obtained result, were summarized as follows: 1. Tracheid transition from springwood to summerwood was abrupt, mel slightly gradual in Taxus cuspidata S. et Z. and summerwood width of Larix gmelinii var. principis-ruprechtii Pilger was wider than that of Taxus cuspidata S. et Z. 2. Normal vertical and horizontal resin canals and surrounding thick epithelial cells were present in Larix gmelinii var. principis-ruprechtii Pilger but not present in Taxus cuspidata S. et Z. 3. Instead of helical thickening, helical chicks were observed only in summerwood trachieds of Larix gmelinii var. principis-ruprechtii Pilger. However, helical thickenings forming S, Z., and horizontal helix perpendicular to long axis of tracheid were observed in springood and summerwood tracheids of Taxus cuspidata S. et Z. and these helical thickenings were considered as analogous to the innermost layer of secondary wall. 4. Uniseriate and fusiform rays were appeared on tangential surface of Larix gmelinii var. principis-ruprechtii Pilger but oly uniserate ray was observed on tangential surface ot Taxus cuspidata S. et Z. The fusiform rays of Larix gmelinii var. principis-ruprechtii Pilger usually contained one horizontal resin canal bot occasionally two horizontal resin canals were contained in a fusiform ray. 5. Trabeculae and crassulae were observed in the tracheids of Larix gmelimii var. principis-ruprechtii Pilger but not observed in the tracheids of Taxus cuspidata S. et Z.

• Korean Journal of Pharmacognosy
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• v.26 no.2
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• pp.116-121
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• 1995

To seek for a stable supply source of taxol as an anticancer agent urgently needed for the treatment of ovarian cancer, the concentrations of taxol and cephalomannine in bark and needles of 37 yew (Taxus cuspidata S. et Z.) trees grown in 6 locations of Cheju Island were analyzed by using HPLC. A considerable variation in the content was observed in plant part and the location. The average taxol content in the needles ($220.3{\;}{\pm}{\;}56{\;}{\mu}g$ per g dry wt) was ca. 4 times hgher than that in the bark, whereas the content of cephalomannine in the needles $(353{\;}{\pm}{\;}141{\;}{\mu}g)$ was ca. 5 times higher than that in the bark. Particularly, even within Cheju Island, needles from trees grown near the coast including Sogwipo area contained much higher taxol contents than needles from trees grown at mountain locations, indicating that taxane level is affected by environmental effects. Taxol in the needles was confirmed by electrospray mass spectrometry. These results suggest that the needles of yew trees growing in Cheju Island may provide a renewable source for taxol.

• Animal Systematics, Evolution and Diversity
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• v.18 no.1
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• pp.85-89
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• 2002

The genus Epitetracnemus Girault, 1915 is reported for the first time from Korea. A new species, E. kosef Li et Byun sp. norm. is described. The species is internal parasitoid wasp on Pseudaulacaspis cockerelli(Cooley) which infests on Taxus cuspidata S. et Z.

• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.365-371
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• 1994

This study was conducted to investigate variations in taxol contents of bark, shoots, needles of three native Taxus species in Korea. Sample materials were collected in early October of 1992 from five individual, trees each of the five populations of Taxus cuspidata located at Mt. Halla, Mt. Jiri, Mt. Deokyu, Mt. Sobaek, and Mt. Taebaek and two individual trees of Taxus cuspitosa in Mt. Seolak and in March of 1993 from the five individual trees of Taxus cuspidata var. latifolia in Ulleung Island. The collected materials were analyzed by HPLC to determine the amount of taxol content. Regardless of species and population, the taxol content was highest in bark and followed by shoots and needles. In Taxars cuspidata the taxol content in the bark was highest for the population of Mt. Halla (0.017%) and decreased in the order of Mt. Jiri, Mt. Sobaek, Mt. Deokyu, and Mt. Taebaek. The taxol content in the shoots was highest for the population of Mt. Halla(0.0053%) and decreased in the order of Mt. Sobaek, Mt. Jiri, Mt. Deokyu, and Mt. Taebaek. The taxol content in the needles was higher for the population of Mt. Halla(0.0013%) than any other populations and decreased in the order of Mt. Taebaek, Mt. Sobaek, Mt. Deokyu and Mt. Jiri. The taxol contents of Taxus caespitosa(0.0035%) were lower than that of Taxus cuspidate in all of the three plant parts. The taxol content of Taxus cuspidate var. latifolia(0.0064%) was higher than that of Taxus cuspidate for shoots and needles, but lower than that of Taxus cuspidate. There was a tendency of increasing taxol contents of bark and shoots of Taxus cuspidate, as latitude decreased. This study indicated that taxol content was relatively high in the populations of Taxus cuspidate. Particularly taxol content in the bark of the Taxus cuspidate in Mt. Halla population was about the same as that of pacific yew, indicating a high potential for commercial use.

• Journal of Korean Society of Forest Science
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• v.70 no.1
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• pp.1-6
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• 1985

The purpose of this experiment lies in finding the most appropriate cutting condition of Taxus cuspidata Sieb. et Zucc. which has been considered valuable tree species in gardening. Statistical analysis was focused on the comparison of the average ratios of rooting between greenhouses and fields, based on the observation rooted autogenis. To conduct this survey, hardwood cutting of Taxus cuspidata Sieb. et Zucc. were performed at both places of experiment above on April 20, 1982 with rooting results calculated on October 31, 1982. After formation of cuttings, it was soaked in IBA 200 ppm for 12 hours and than put in a bed. A summary of the result is presented as follows; 1) Cutting at the greenhouse showed higher average rate of rooting than at fields. Same tendency was found regardless of the differences in treatment. 2) It was clear that IBA-treatment contributed remarkably to causing higher average rooting rate. As an evidence, when twenty centimeter cuttings were inserted sandy media, IBA-treated area resulted in eighty six percent of average rooting rate, as compared with only twenty three percent in nontreatment case. In case of field cutting, IBA-treatment brought about fifty three percent of rooting in comparison with eleven percent nontreatment. 3) When sandy soil, loam and brown soil were separately used as cutting media, the highest rooting rate was found in case of sandy soil, without any difference between the two experimental places above. 4) As a result of the analysis to seek the impact of the length of cuttings on rooting, the range of length form fifteen to twenty centimeter was apparently most appropriate. It was also found that the rate of rooting declined beyond twenty five centimeter. 5) Two kinds of rooting pattern were observed. One was the case that callus cell lump was created on the lower cut side of cuttings. Importantly, root radical were formed inside the lump to influence the germination of root system. The other relates to the case that adventitious root which look like lateral roots appeared at the stem region. In abstract, first, sandy soil was effectively recommended in case of hardwood cutting in April. Second, the most appropriate length of cuttings ranged between fifteen and twenty centimeters. Third, high density IBA treatment was clearly effective. Forth, for proper environmental management, both pre-disinfection of sail by sterilizer and maintenance of high relative humidity were essentially required.

• Conservation Science in Museum
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• v.10
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• pp.43-61
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• 2009

The Buyeo National Museum was requested conservation treatment for wooden objects excavated from three Baekje archeological sites: Neungsan-ri, Ssangbuk-ri, and Gungnamji Pond. Prior to conservation treatment, analysis was conducted to identify the species used. The results of the analysis revealed wood from diverse species of trees including Hard pine, Cryptomeria japonica D. Don, Zelkova serrata Makino, Quercus spp., Platycarya strobilaceae S. et Z., Castanea spp., Torreya nucifera S. et Z., Taxus cuspidata S. et Z., and Salix spp. A high percentage of the objects were made of Cryptomeria japonica D. Don., a species native to Japan, which indicates that exchange with Japan was active at that time. Among the wooden objects, we analyzed lacquer fragments from six pieces of lacquerware, and the characteristics of the lacquer fragments were peculiar to specific artifacts. Most of the fragments were thicker than 100 ㎛. Pure lacquer and mixed black pigment were used. Infrared spectroscopy of the lacquered wooden fragments revealed that they had a very similar absorption band as refined lacquer, confirming that they were painted with lacquer. For their conservation, we immersed the objects in a high molecular weight aqueous solution of PEG#3,350 (10% → 50%) to strengthen them before vacuum freeze-drying.

• Journal of Korean Society of Forest Science
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• v.79 no.4
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• pp.359-366
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• 1990

This study was carried out to investigate rooting rate, plagiotrophic growth and root-promoting effect of IBA in Taxus cuspidata S, et Z. cuttings. The results obtained were summarized as follows ; 1. As the ortet ages of cuttings were increased in 5, 10, to 20 years, the rooting rates were decreased in order of 85.7%, 81.7% and 62.4%, and the number of primary roots were also decreased in 5.3, 3.7 and 2.9, respectively. 2. Rooting rate by cutting types of lateral shoot were higher than those of main shoot type, significant at the 1% level. 3. IBA(l00ppm) treatment promoted the rooting rates and the number of primary roots. 4. As the ortet ages were increased in 5, 10 to 20 years, angles of central shoot of rooted cuttings which mesured cyclophysis were decreased in order of $75.9^{\circ}$, $68.5^{\circ}$ and $59.6^{\circ}$, respectively, significant at the 1% level. 5. Average angles showed $77.0^{\circ}$ for main shoot cutting, $65.9^{\circ}$ for upper lateral shoot and $61.7^{\circ}$ for lower lateral, which was significant at 1% level, and lateral shoot cutting showed more topophysis than main shoot cutting. 6. Shoot length of rooted cuttings from main shoot cutting was longer than that from lateral shoot significant at the 1% level. 7. Relationship between some characteristics showed highly positive correlation.