• Microbiology and Biotechnology Letters
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• v.34 no.2
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• pp.101-108
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• 2006

YNB54 strain which shows inhibitory activities specific to the plant pathogenic Phytophthora sp. on potato dextrose agar medium was screened among lots of strains isolated from Korean soils. To identify taxonomy of the Phytophthora specific antagonistic bacteria YNB54, 165 rDNA sequence, MIDI fatty acid composition, DNA-DNA hybridization, GC content, and commercial multitest systems such as API 20E and Biolog GN were performed. Results of commercial kits including lots of biochemical and physiological reactions showed that this strain was closely related to taxa including Enterobacter cloacae and Enterobacter cancerogenus species than other genera(Citerobacter Klebsiella, Leclercia). Also, analysis of its MIDI, G+C contents, and DNA-DNA hybridization suggests that this strain was more similiar to the Genus Enterobacter than other genera (Citerobacter Klebsiella, Leclercia). This strain was potentially identified as Enterobacter sp. by these results. But our 16S ribosomal DNA sequences (rDNA) analysis confirmed that it was more closely related to the cluster of Citerobacter freundii ATCC 29935 than any other Enterobacter species. In the absence of defined phylogenetic critia for delineating genera, the results observed with Citrobacter and Enterobacter species suggest that further studies are needed to clarify their relationships. This investigation demonstrates that YNB54 strain is genetically diverse and potentially more taxonomically complex than hitherto realized. Further study is necessary to confirm their taxonomic positions.

• Korean Journal of Plant Taxonomy
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• v.37 no.4
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• pp.431-446
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• 2007

The purpose of this study is to review the taxonomical position of Phytolacca insularis Nakai based on morphological characters and ITS sequences. Phytolacca insularis was similar to P. acinosa in the stem shape, inflorescence, apocarpous, eight pistils, eight stamens, pink anther, and eight fruits. But the unique difference between P. insularis and P. acinosa was leaf size and pistil color. Phytolaccri insularis and P. acinosa have a similar sculpturing patterns as an foveolate and microscabrate in pollen, but they differed from P. americana in having a bigger foveolae size in sculpture pattern. Phytolacca insularis and P. acinosa were similar in the seed size but different from P. americana by having a small size of seed. The seed surface of P. insularis and P. acinosa was similar with a irregularly undulate shape, but its feature of P. americana differed in having the reticulate shape. Phytolacc a insularis had identical ITS sequences with P. acinosa. Phytolacca insularis and P. acinosa have a close relationship in the morphological characters and ITS sequences. The examinations of morphology and ITS sequences suggest that P. insularis be a form of P. acinosa although it has been formerly treated as a distinct species.

• The Korean Journal of Zoology
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• v.32 no.3
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• pp.242-251
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• 1989

Morphometric and genetic analyses on six populations of the two subspecies of Cobitis koreensis were performed to investigate the geographic variation and to clarify their taxonomic status. No significant differences between subspecies were found. Puan population of C. k. pumilus tends to be smaller in numbers of cross bars and dorsal spots, than those of C. k. koreensis populations with no statistical significance. Average degree of genic variation among six populations of the two subspecies was A=1.5, P =37.3%, HD=0.053, and HG=0.097. Marginal populations of Samchog (C. k. koreensis) and Puan(C. k. pumilus) were least variable: A= 1.3, P=23.8%, HD=0.0l0, HG=0.043, and A= 1.2, P=19.0%, HD=0.029, HG=0.078 respectively.The difference between HD and HG in all populations may indicate a patchness distribution between demes within each population. Genetic similarities between the two subspecies were, on the average, S=0.894, and this value correspond to the average genetic similarities among five populations of C. k. koreensis (5=0.899). Chongup population of C. k. koreensis and Puan population of C. k. pumilus was most similar genetically (5 =0.931). The results of morphometric and genetic analysis in this study indicate that C. k. pumilus is not a valid subspecies and should be considered as a population of C. koreensis.

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.7-13
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• 1989

This study was executed to investigate characterization of physiological genetic in Coriolus versicolor, basidiomycetes. The optimal media for mycelia culture were PDA, CVT-I and MES as solid media, 927 and CVT-III were good as liquid media, respectively. The optimal condition for mycelial culture was pH 5.6 and $25-30^{\circ}C$. Electrophoretic isozymes and protein patterns from mycelia identified very similar in 16001 and KD88001 strain, but the other species were very diffrent in band patterns. Especially, pattern of esterase seemed to be valuable tool as taxonomic techniques for indentifying species of Coriolus versicolor. Fruiting body was cultivated with artificial logs cultivation method; 16001 and KD88001 were very similar to fruiting body shapes and colours but 16001 and CVT-80 were different in their shapes, colours and making primordia. Therefore, 16001 and KD88001 were assumed to the same strain, but 16001, 16002 and CVT-80 had the different genetic background.

• Journal of the Korean Wood Science and Technology
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• v.38 no.4
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• pp.359-374
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• 2010

This study was carried out to investigate the chemotaxonomical correlation and chemical constituents of domestic Quercus spp. barks. The barks of Q. mongolica, Q. aliena, Q. serrata, Q. acutissima, Q. dentata, and Q. variabilis were collected in the experimental forest of Kangwon National University. The combined extracts were successively fractionated with n-hexane, methylene chloride and ethyl acetate using a separation funnel. A portion of the ethyl acetate and H2O soluble materials of each species were chromatographed on a Sephadex LH-20 column using various aqueous MeOH and EtOH-hexane as washing solvents. Spectrometric analysis such as NMR and MS, including TLC, were performed to characterize the structures of the isolated compounds. Ellagic acid (0.03 g), (+)-catechin (4.59 g), taxifolin (3.35 g), and glucodistylin (20.52 g) were isolated from Q. mongolica bark. Gallic acid (0.18 g), (+)-catechin (8.52 g), (+)-gallocatechin (0.09 g), taxifolin (0.54 g), and glucodistylin (3.28 g) were characterized from Q. acutissima bark. Gallic acid (0.38 g), ellagic acid (0.11 g), (+)-catechin (2.01 g), (+)-gallocatechin (0.12 g), and glucodistylin (0.39 g) were identified from Q. dentata bark. Ellagic acid (1.51 g), (+)-catechin (21.91 g), and glucodistylin (3.91 g) were purified from Q. aliena bark. Ellagic acid (0.84 g), (+)-catechin (0.82 g), taxifolin (4.02 g), and glucodistylin (21.50) were isolated from Q. serrata bark. Gallic acid (0.24 g), caffeic acid (0.05 g), (+)-catechin (0.32 g), and glucodistylin (0.65 g) were purified from Q. variabilis bark. (+)-Catechin and glucodistylin were isolated from all the barks. Glucodistylin can be a taxonomic index on Quercus spp.

• 한국해양학회지
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• v.29 no.3
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• pp.304-318
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• 1994

Macrobenthic fauna in three Keum-Mankyung-Dongjin Estuaries and their nieghbouring coastal region in the west coast of Korea were sampled as 39 stations with van Veen grab to describe the distribution patterns of macrobenthos. Total 61 taxa from 10 faunal taxonomic groups and 5,636 individuals were collected. Abundance, species number, and faunal composition varied among three estuaries and coastal region. Dominant species from estuaries were similar to each other, but different from those of coastal region. Dominant species in estuarine regions were Prionospio cirrifera, potamocorbula amurensis. Nephtys californiensis, Glycera chiron and Glycinde sp, those from coastal area were Mactra chinensis. Magelona japonica, Owenia fusiformis. Anaitides Korean and Nephtys polybranchia. Two most dominant species in estuaries, Potamocorbula amanuensis and Prionospio cirrifer were not found elsewhere in Korean waters. The patchy distribution of dominant species and low species richness were reflected in the low species diversity Filter feeders were a major component in estuaries. High proportion of carnivores and low percentage of subsurface deposit feeders seemed to be a typical trophic composition in the study area. the distribution pattern of macrobenthos was related with the salinity and sediment types.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.153-159
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• 2011

This study was carried out to know the insect fauna of turf grass area in forest. In Korea, graves and public cemetery in mountain areas are usually covered by turf grass which were mainly examined. There were 86 species belonging to 77 genera of 39 families in 11 orders. Among them, site 3 had highest occurrence (38%), second was site 2 (36%) and in the last place was site 1 (26%). Hemiptera revealed best species composition (29.1%) and came Coleoptera (19.8%), Orthoptera (15.1%), Lepidoptera (12.8%) and Odonata (5.8%) etc. The reason why Hemiptera and Orthoptera are mainly found in these areas is because they usually feed on short plants that grow in there. Taxonomic species composition, occurrence ratio by site and species and comparison analysis were provided.

• Korean Journal of Plant Taxonomy
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• v.39 no.1
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• pp.35-41
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• 2009

Astilbe (Saxifragaceae) is a genus well known for its disjunctive distribution in Asia and eastern North America. In this study, we reconstructed a molecular phylogeny of the genus using the sequences of ITS regions of nuclear ribosomal DNA. A total of 17 species representing major lineage of Astilbe and closely related taxa were included in the phylogenetic analyses. We obtained a Bayesian phylogenetic tree in which Saxifragopsis was positioned as a sister group to Astilbe. The Japanese endemic species, A.platyphylla was the most basal lineage within the genus. This species is well known for its distinct morphological features such as unisexual flowers, apetaly, and calyx with 7-11 lobes. Two species, A. biternata, a New World representative of the genus, and A. rivularis widely distributed in S. Asia, branched off early in the evolution of Astilbe. The remaining species formed a strongly supported core clade, which diverged into two robust geographical lineages: the first ("Japonica" clade) of species distributed in Japan, Taiwan, and Philippines and the other ("Rubra" clade), of taxa in China and Korea. The ITS phylogeny indicates that the Bering land bridges were the major route for the origin and dispersal of A. biternata. The two Taiwanese taxa and A. philippinensis were found to derive from the Japanese member, as the genus advanced southwards. The ITS phylogeny suggests that apetaly originated independently at least two times within the genus. Our results do not support Engler's classification system of the genus based on the leaf type (simple vs. compound), but reaffirm Hara's taxonomic idea which primarily considered the features of calyx.

• Korean Journal of Plant Taxonomy
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• v.43 no.2
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• pp.106-117
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• 2013

Using scanning electron microscope (SEM), we examined the trichomes on leaf and petiole and the epicuticular waxes on leaf surfaces for a total of 27 taxa representing two subtribes, Fragariinae and Potentillinae, of tribe Potentilleae (Rosaceae) in Korea. Four types of trichomes on adaxial and abaxial surface of leaves and petioles were identified. Type I (conical hirtellous) is the most common trichome type found in the majority of taxa in Fragariinae and Potentillinae. Type II (verruculose conical hirtellous) can be found only in Potentilla cryptotaeniae of sect. Conostylae of Potentillinae. Potentilla chinensis complex (sect. Conostylae) and P. egedii (sect. Letostylae) have type III trichome (crispate villous), while type IV (floccose villous) can be found in two species in sect. Conostylae, P. nivea and P. discolor. Both woolly hairs and conical hirtellous exist together in types III and IV. The same type of trichomes in leaves and petioles can be found across different subtribes and sections. In addition, different types of trichomes can be found even in a single species. Among the taxa which have type I trichome, the majority of subtribe Fragariinae and P. centrigrana and P. dickinsii complex have well developed epicuticular waxes on the surface of leaves. Sharing epicuticular waxes among the taxa across different subtribes appears to be correlated with their similar geographical distribution and ecological conditions. However, molecular phylogenetic study implies that the existence of epicuticular waxes could be also due to phylogenetic signal.

• Parasites, Hosts and Diseases
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• v.36 no.4
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• pp.217-225
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• 1998

A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus). and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker. and at inner side of the lip one pair of small and two pairs of large type I sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four- week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer＇s canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species. which may be of taxonomic significance.