• 한국식품영양과학회지
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• 제38권12호
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• pp.1712-1717
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• 2009

본 연구는 capsaicin 투여가 콜레스테롤을 포함한 지방의 소장 흡수율에 어떠한 영향을 미치는지를 조사하기 위해서 설계되었다. 지방의 소장 흡수율을 측정하기 위해서 흰쥐 소장 지방 흡수율 측정모델을 이용하여, 쥐의 십이지장으로 capsaicin이 포함된 지질유화액을 8시간 동안 지속적으로 주 입하면서 8시간 동안 림프관으로 분비되는 14C-cholesterol, α-tocopherol, 지방산을 분석하여 capsaicin에 의한 영향을 비교하였다. 8시간 동안 분비된 림프액의 양은 대조군과 capsaicin군 간에 유의적인 차이를 나타내지 않았다. α-tocopherol의 흡수율에서도 유의적인 차이가 없었다. 그러나 총 8시간 그리고 시간대별 14C-cholesterol 흡수율은 대조군과 비교하였을 때 capsaicin군에서 유의적인 차이로 감소되는 경향을 보였다. 또한 중성지방의 흡수율의 비교에서도 팔미트산, 스테아르산, 아라키돈산, DHA의 흡수율은 두 군 간에 유의적인 차이가 없었으나 지방유화액을 통해 주입한 올레인산의 흡수율에서는 유의적인 감소현상을 보였다. 결론적으로, capsaicin의 응용성은 비만 치료 및 지방대사 조절과 관련해 특히, 소장 지방 흡수대사에 치중한 식이적인 수단의 근거로써 활용이 기대된다.

• 약학회지
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• 제34권4호
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• pp.238-243
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• 1990

Pharmacokinetic characteristics of recombinant human interleukin-2 (rH IL-2) wre studied in the rat. First, different doses of rH IL-2 ranging from 6,400 to 1,600,000 U/kg were injected intravenously and the effect of dose size on the pharmacokinetics was examined. There was no dose dependency in the pharmacokinetics of rHIL-2 in the dose range of 6,400-40,000 U/kg. But at the dose of 1,600,000 U/kg, there was a severe hemolysis throughout the experiment and the pharmacokinetic parameters such as Vdss and CLt were significantly increased compared to those obtained from lower doses. It also showed that this drug is hardly distributed to the peripheral tissues and hardly eliminated from the body, since the valume of distribution (Vdss) and total body clearance (CLt) were 45-75 ml/kg and 1-2 ml/min/kg, respectively. The Vdss is close to the actual plasma volume and the CLt is less than glomerular filtration rate (GFR). Therefore it seemed that rH IL-2 is distributed only in the plasma pool and hardly filtered in the kidney due to its very large molecular weight. Second, rH IL-2 was administered to the rat via several routes such as hepatic portal vein (PV), intraperitoneal (IP), peroral (PO) and intranasal (IN) routes. The bioavailabilities (BA) of PV, IP, PO and IN routes were 96.8, 4.9, 0 and 0.1%, respectively. The addition of some nasal absorption enhancers such as taurocholate, taurodeoxycholate, glycocholate and glycodeoxycholate did not increase the BA of intranasaly administered rH IL-2. The result is contrast to the effect of these bile salts on the nasal absorption of ${\alpha}-inteferon$. Considering it together with the pharmacokinetic parameters, very large molecular weight of rH IL-2 seemed again to be the cause to very poor membrane permeability.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1376-1383
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• 2018

The hepatitis B virus (HBV) envelope contains small (S), middle (M), and large (L) proteins. PreS1 of the L protein contains a receptor-binding motif crucial for HBV infection. This motif is highly conserved among 10 HBV genotypes (A-J), making it a potential target for the prevention of HBV infection. In this study, we successfully generated a neutralizing human monoclonal antibody (mAb), 1A8 (IgG1), that recognizes the receptor-binding motif of preS1 using a phage-displayed human synthetic Fab library. Analysis of the antigen-binding activity of 1A8 for different genotypes indicated that it can specifically bind to the preS1 of major HBV genotypes (A-D). Based on Bio-Layer interferometry, the affinity ($K_D$) of 1A8 for the preS1 of genotype C was 3.55 nM. 1A8 immunoprecipitated the hepatitis B virions of genotypes C and D. In an in vitro neutralization assay using HepG2 cells overexpressing the cellular receptor sodium taurocholate cotransporting polypeptide, 1A8 effectively neutralized HBV infection with genotype D. Taken together, the results suggest that 1A8 may neutralize the four HBV genotypes. Considering that genotypes A-D are most prevalent, 1A8 may be a neutralizing human mAb with promising potential in the prevention and treatment of HBV infection.