• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권6호
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• pp.515-525
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• 2005

Purpose of study: Lingual nerve damage can be caused by surgery or trauma such as physical irriatation, radiation, chemotherapy, infection and viral infection. Once nerve damage occurred, patients sometimes complain taste change and loss of taste along with serious disturbance of tongue. The purpose of this study was to evaluate the effects of unilateral lingual nerve transection on taste as well as on the maintenance of taste buds. Materials & Methods: Male Sprague-Dawley rats weighing 220-250g received unilateral transection of lingual nerve, subjected to the preference test for various taste solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) with two bottle test paradigm at 2, 4, 6, or 8 weeks after the operation. Tongue was fixed with 8% paraformaldehyde. After fixation, they were observed with scanning electron microscope(JSM-$840A^{(R)}$, JEOL, JAPAN) and counted the number of the dorsal surface of the fungiform papilla for changes of fungiform papilla. And, Fungiform papilla were obtained from coronal sections of the anterior tongue(cryosection). After cryosection, immunostaining with $G{\alpha}gust$(I-20)(Santa Cruz Biotechnology, USA), $PLC{\beta}2$(Q-15)(Santa Cruz Biotechnology, USA), and $T_1R_1$(Alpha Diagnostic International, USA) were done. Immunofluorescence of labeled taste bud cells was examined by confocal microscopy(F92-$300^{(R)}$, Olympus, JAPAN). Results: The preference score for salty and sweet tended to be higher in the operated rats with statistical significance, compared to the sham rats. Fungiform papilla counting were decreased after lingual nerve transaction. In 2 weeks, maximum differences occurred. Gustducin and $T_1R_1$ expressions of taste receptor in 2 and 4 weeks were decreased. $PLC{\beta}2$ were not expressed in both experimental and control group. Conclusion: This study demonstrated that the taste recognition for sweet and salty taste changed by week 2 and 4 after unilateral lingual nerve transection. However, regeneration related taste was occurred in the presence of preserving mesoneurial tissue and the time was 6 weeks. Our results demonstrated that unilateral lingual nerve damage caused morphological and numerical change of fungiform papilla. It should be noted in our study that lingual nerve transection resulted in not only morphological and numerical change but also functional change of fungiform papillae.

• International Journal of Oral Biology
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• 제31권3호
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• pp.99-105
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• 2006

Von Ebner's glands (vEG) are minor salivary glands associated with circumvallate and foliate papilla. The secretions of vEG consist of microenvironment of the taste buds in the circumvallate and foliate papillae, and thus saliva from vEG plays a role in the perception of taste. The $Ca^{2+}$ signaling system in rat vEG acinar cell was examined using the $Ca^{2+}$-sensitive fluorescent indicator Fura-2. Agonist-induced increase in intracellular $Ca^{2+}\;([Ca^{2+}]_i)$ was stimulated by carbachol (CCh) and substance P (SP), but not by norepinephrine (NE), and recovered to control levels by their receptor antagonists dose-dependently. The effects were also observed in $Ca^{2+}$-free medium, suggesting mobilization from intracellular $Ca^{2+}$ store. These results in the vEG acinar cell indicate that 1) $[Ca^{2+}]_i$ is at least regulated by muscarinic and neurokininergic (NK1) receptors; 2) the increases in $[Ca^{2+}])i$ activated by CCh and SP are mainly mediated by discharge of cytosolic calcium pool.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.213-218
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• 2008

Swertia chirata is an endangered gentian species that prefers to grow at higher altitudes. This ethnomedicinal herb is known primarily for its bitter taste caused by the presence of important phytochemicals that are directly associated with human health benefits. Due to a continuous loss of habitat and inherent problems of seed viability and seed germination, alternative strategies for propagation and conservation are urgently required to prevent the possible extinction of this species. We have formulated a reproducible protocol for the rapid propagation and conservation of this plant using leaves taken from in vitro shoot cultures. Direct induction of more than seven shoot buds per explant was achieved for the first time when the explants were placed on MS medium supplemented with $2.22{\mu}M$ N-6-benzyladenine, $11.6{\mu}M$ kinetin, and $0.5{\mu}M$ ${\alpha}-naphthalene$ acetic acid. Direct organogenesis was noted exclusively from the adaxial surface of the basal segments of leaves. Leaves closer to the apical meristem were more responsive than those farther away from the meristem. Plants raised through direct organogenesis were evaluated for their clonal fidelity by chromosomal analysis and DNA fingerprinting. Complete plants were successfully transferred to the field condition and produced viable seeds. Given the enormous potential of this age-old medicinal plant in terms of potential health-benefitting drugs, this protocol can be used for commercial propagation purposes and to initiate future genetic improvement studies.

• Journal of Community Nutrition
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• 제6권1호
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• pp.42-47
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• 2004

The purpose of the study was to investigate assisted living residents' perception of foodservice experience using a qualitative approach. A total of 14 residents who lived in assisted living units of a continuing care retirement community participated in interview sessions. The interviews were semi-structured with open-ended questions. The interviews were transcribed and coded to identify themes from the responses. The residents perceived the availability of choices, variety of foods, and service positively, but expressed concerns about food preparation, food quality, and repetitive menus. They were particularly concerned with tough and dry meats. The foodservice department should consider the older residents' ability to chew and their changing taste buds when determining quality of food purchased and preparation methods. The residents considered the service pleasing and described it as satisfactory. They stated that the service staff was friendly but needed more training in proper service techniques. Communication between the foodservice department and the residents was an area to be improved. The foodservice department should increase the residents' involvement in the menu planning and evaluation of the foodservice on a routine basis. Foodservice managers and dietitians working for the older adults can utilize the findings of the study to identify strategies to improve foodservice at their facilities

• Journal of Community Nutrition
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• 제6권1호
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• pp.41-47
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• 2004

The purpose of the study was to investigate assisted living residents' perception of foodservice experience using a qualitative approach. A total of 14 residents who lived in assisted living units of a continuing care retirement community participated in interview sessions. The interviews were semi-structured with open-ended questions. The interviews were transcribed and coded to identify themes from the responses. The residents perceived the availability of choices, variety of foods, and service positively, but expressed concerns about food preparation, food quality, and repetitive menus. They were particularly concerned with tough and dry meats. The foodservice department should consider the older residents' ability to chew and their changing taste buds when determining quality of food purchased and preparation methods. The residents considered the service pleasing and described it as satisfactory. They stated that the service staff was friendly but needed more training in proper service techniques. Communication between the foodservice department and the residents was an area to be improved. The foodservice department should increase the residents' involvement in the menu planning and evaluation of the foodservice on a routine basis. Foodservice managers and dietitians working for the older adults can utilize the findings of the study to identify strategies to improve foodservice at their facilities.

• 한국식품영양과학회지
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• 제32권1호
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• pp.58-61
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• 2003

옥로차 제조에 쓰인 차나무 잎은 백아중이 29.4 g,출개도 77.8%, 엽수분이 79.5%였다. 75$^{\circ}C$, 30분 처리가 다른 처리 조건에 비해 완제품의 수율이 높았으며, 총질소, theanine, glutamic acid, arginine 엽록소 함량이 많았고, 탄닌함량이 낮았다. 또한 75$^{\circ}C$, 30분 정유처리가 관능평가에서 타처리보다 6~12점 더높아 양호하였다. 결론적으로 75$^{\circ}C$, 30분 정유처리가 제다품질 및 관능평가에서 우수하여 가장 양호한 정유조건이었다.

• 생물환경조절학회지
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• 제15권1호
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• pp.107-113
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• 2006

These studies were carried out to investigate the quality of green tea processed by different pan-firing hand rolling and to select the best conditions during the procedure of green tea processing the results were as follows. At harvestion tea plant leaf, tea plants had 4 leaves. each leaf size was 7.5 cm'$\square$2.6 cm, and weight of 100 buds 46.6 g. The increase of pan-fired time decreased in the moisture content and yield of green tea. After processing green tea, 5 times pan-fired one had much more contents of total nitrogen (T-N:5.31%), caffeine (2.00%) and total amino acid (T.A.A. : 3,046 mg%) and less content of tannin (13.41%) than the other treatments. but 3 times pan-fired one had the highest contents of vitamin C (204.3 mg%) and chlorophyll (365.0 mg%) among three kinds pan-firing method. Sixteen kinds of free amino acid including theanine as a good taste component of pan-fired green tea were isolated and measured by HPLC. Free amino acid (FAA) contents were in the range of $2,683{\sim}2,925$ mg% and among various pan-firing methods, 5 tims pan-fired one had the highest content of F.A.A. Among these free amino acids, theanine occupied as much as $56.4{\sim}61.3%$ of F.A.A. Lightness, yellowness and redness of 5 times pan-fired green tea were middle value among three different treatments. Besides, it gained the highest score of sensory evaluation among them. These above results suggested that 5 times pan-fired green tea was considered to be the best level as pan-firing times of green tea processing.

• 한국어류학회지
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• 제17권3호
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• pp.167-172
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• 2005

조간대와 강하구에 출현하는 미끈망둑 Luciogobius guttatus은 썰물 동안에 건조하기 쉬운 자갈 바닥 아래에서 서식하는 특징을 보인다. 이러한 서식처를 선호하는 미끈망둑의 호흡체계를 조사하기 위해 지느러미 및 흡반을 포함하는 5개의 부속지의 표피구조를 관찰하였다. 표피는 모두 맨바깥층(outermost layer), 중간층(middle layer), 기저층(stratum germinativum)으로 구성된다. 맨바깥층은 다형세포 또는 편평세포, 그리고 점액세포로 구성되었으며, 특히 단일세포성의 점액세포는 $11.1{\sim}16.1{\mu}m$의 크기로 1~2층으로 배열되어 있으며, 산성다당류로 확인되었다. 중간층은 이웃한 표피세포로 팽대해진 팽대세포 (swollen cell)로 구성되어 있으며, $12.3{\sim}15.2{\mu}m$의 크기로 1~11층으로 배열되어 마치 거미망구조를 보인다. 이러한 팽대세포로 인해 상피 두께의 대부분을 차지한다. 많은 모세혈관이 기저막 아래에 분포하고 있으며, 미뢰 (taste bud)가 표피에 존재한다. 이러한 표피의 특징은 미끈망둑이 이중호흡체계인 피부호흡 (cutaneous respiration)과 밀접한 관계가 있는 것으로 생각된다.

• 한국약용작물학회지
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• 제7권2호
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• pp.101-106
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• 1999

덖음차 제조공정중 첫 덖음시간에 따른 최적 제다조건을 구명한 실험결과는 다음과 같다. 채엽시 엽수는 4매, 엽장 및 엽폭은 $6.4cm\;{\times}\;2.4cm$, 백아중(白芽重)은 40.2g이었다. 첫 덖음시간이 길어질수록 수분함량은 감소하여 6분 덖음시 6.0%에서 14분 덖음시 4.6%로 되었고, 제품수율은 10분에서 20.12%로 가장 높았다. 덖음시간에 따라 전질소는 $5.14{\sim}5.41%$, 총아미노산은 $2,531{\sim}3,282mg/100g$의 변이를 보였으며, 그 중에서 첫 덖음시간이 10분일 때 가장 함량이 높았던 반면, 탄닌은 $13.22{\sim}13.86%$로 10분 처리에서 함량이 가장 낮았다. 카페인 $1.64{\sim}2.01%$로 첫덖음시간에 별로 영향을 받지 않았으며, 엽록소($319.1{\sim}380.3mg/100g$)와 비타민 C ($158.9{\sim}208.8mg/100g$)는 덖음시간이 길어질수록 함량이 감소하는 경향을 보였다. 16종의 유리아미노산 및 질소화합물 분석 결과 총 함량은 $2,447{\sim}3,192mg/100g$이었으며 10분 첫 덖음이 가장 높은 함량을 나타냈다. 이들 중에서 theanine이 50% 이상이었고 aspartic acid, serine, glutamic acid 및 arginine을 합치면 $89.4{\sim}90.0%$였다. 차의 색깔은 덖음시간이 길어질수록 황색이 증가하였고 녹색은 10분 덖음에서 가장 진한 색을 나타냈다. 또한 관능검사 결과도 10분 덖음이 가장 높은 평가를 받았다. 이상의 결과로 보아 첫 덖음 시간의 경우 10분이 적당하리라 생각된다.

• 수산해양교육연구
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• 제18권2호
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• pp.164-171
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• 2006

본 연구에서는 부산지역의 생선횟집을 중심으로 생선 매운탕의 조리 실태를 조사하여 표준조리법 개발에 기초자료로 활용하고자 하였다. 1. 재료로 사용되는 어류는 조피볼락과 넙치가 공통으로 사용되며, 그 외 어종들을 사용하는 것으로 나타났다. 2. 야채류는 무, 대파, 고추, 양파, 조미 및 향신료는 고춧가루, 마늘, 소금, 후추가 공통으로 사용되고 있는 것으로 나타났으며, 기타 재료들이 부가적으로 사용되는 것으로 나타났다. 3. 생선 매운탕에 사용되는 물은 생수와 어류를 삶은 물을 이용하고 있으며, 기타 야채를 삶은물을 이용하는 곳도 있는 것으로 나타났다. 4. 3-4인분 기준의 생선 매운탕에 사용되는 생선의 양은 조피볼락 1.5-1마리와 넙치 0.5-1 마리에 해당되는 머리와 뼈를 이용하는 것으로 나타났다. 5. 생선 매운탕의 조리방법은 물 끓인 다음 재료를 이용하며, 양념장은 양산품을 바로 이용하는 것으로 나타났으며, 조리기구는 대부분 뚝배기를 이용하는 것으로 나타났다.