• KSBB Journal
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• v.31 no.4
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• pp.284-290
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• 2016

In this research, recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced by transgenic rice cells. RAmy3D promoter was used for overcome the limitation of low expression level in transgenic plant cells, and the secretion of target protein was accomplished by signal peptide. However, the RAmy3D promoter system which can be induced only by sugar starvation causes the decrease of cell viability. As a result, cell death promotes the release of protease which degrades the target proteins. The protein stability and productivity can be significantly influenced by proteolysis activity. Therefore, development of new strategies are necessary for the in situ recovery of target proteins from cell culture media. In this study, in situ recovery was performed by various strategies. Direct addition of Protein A resin with nylon bag leads to cell death by increased shear stress and decrease in production of hCTLA4Ig by protease. Medium exchange through modified flask could recover hCTLA4Ig with high cell viability and low protease activity, on the other hand, the productivity was lower than that of control. When in situ recovery was conducted at day 7 after induction in air-lift bioreactor, 1.94-fold of hCTLA4Ig could be recovered compared to control culture without in situ recovery. Consequently, in situ recovery of hCTLA4Ig from transgenic rice cell culture could enhance productivity significantly and prevent degradation of target proteins effectively.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1657-1667
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• 2007

This study propose a model for railway safety evaluation with which the safety of whole railway system can be evaluated. The evaluation model is to generate a safety index which quantitatively represent the degree of railway safety. Safety index is proposed a function of three indexes; an accident index, safety management index, and safety culture index. This paper describes the first result from the study on the safety target which will be a key starting point toward the development of safety evaluation model. It is recommended that the safety target be composed of several sub-targets that are apportioned to constituent components. It is concluded that the classification of safety target influence on deciding components or attributes that constitute each sub-indexes; an accident index, safety management index, and safety culture index. Based on this study, a railway safety evaluation model will be developed in the next study.

• International Journal of Railway
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• v.5 no.4
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• pp.175-181
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• 2012

This study proposes a model for railway safety assessment with which the safety of whole railway system can be evaluated. The purpose of the assessment model is to generate safety indicators which quantitatively represent the degree of railway safety. Safety indicators were proposed as three indicators according to their functions; accident indicators, safety management indicators, and safety culture indicators. This paper describes the first result on the safety target which will be a key starting point toward the development of safety assessment model. It is recommended that the safety target to be composed of several sub-targets are apportioned to constituent components. It is concluded that the classification of safety target has influence on deciding components or attributes that constitute each sub-indicators; accident indicators, safety management indicators, and safety culture indicators. Based on this study, a railway safety assessment model will be developed in the following study.

• International Journal of Advanced Culture Technology
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• v.11 no.2
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• pp.381-388
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• 2023

Small-scale environmental impact assessments have limitations in terms of survey duration and evaluation resources, which can hinder the assessment and analysis of the current situation. In this study, we propose the use of drone technology during the environmental impact assessment process to supplement these limitations in the current situation analysis. Drone photography can provide rapid and accurate high-resolution images, allowing for the collection of various information about the target area. This information can include different types of data such as terrain, vegetation, landscape, and real-time 3D spatial information, which can be collected and processed using GIS software to understand and analyze the environmental conditions. In this study, we confirmed that terrain and vegetation analysis and prediction of the target area using drone photography and GIS analysis software is possible, providing useful information for environmental impact assessments.

• Animal cells and systems
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• v.8 no.2
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• pp.125-133
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• 2004

We have extended our previous work that cross-linking CD4 molecules using specific MAb induced antigen nonspecific, MHC unrestricted killing of virally infected target cells by CD$4^+$We have extended our previous work that cross-linking CD$4^+$ molecules using specific MAb induced antigen nonspecific, MHC unrestricted killing of virally infected target cells by CD$4^+$ T cells. The killing activity of antibody activated CD$4^+$T cells was completely blocked by herbimycin A, a protein tyrosine kinase (PTK) inhibitor, but not by bisindolylamaleimide, a protein kinase C (PKC) inhibitor. Herbimycin A treated human or bovine peripheral blood CD$4^+$T cells lacked PTK activity and failed to kill virally infected target cells even after cross-linking of CD4 molecules. The CD$4^+$cross-linking failed to induce effector cell proliferation or the transcription of TNF${\beta}$ Upregulation of TNF${\beta}$ was induced by incubating the antibody activated effector cells with BHV-1 infected D17 target cells for 10 h. Anti-TNF${\beta}$ antibody partially abolished (13-44%) the direct effector cell-mediated antiviral cytotoxicity. However, this antibody neutralized 70 to 100% of antiviral activity of effector and target cell culture supernatants against BHV-1 infected D17 cells. The inhibition level of the antiviral activity by the antibody was dependent on the effector and target cell ratio. These results support the hypothesis that increased p$56^ICK enzyme activity in effector cells transduces a signal critical for effector cell recognition of viral glycoproteins expressed on the target cells. Following target cell recognition, lytic cytokines known to participate in target cell killing were produced. A better understanding of the killing activity displayed by CD$4^+$T lymphocytes following surface receptor cross-linking will provide insight into the mechanisms of cytotoxic activity directed toward virally-infected cells.T cells. The killing activity of antibody activated CD$4^+$T cells was completely blocked by herbimycin A, a protein tyrosine kinase (PTK) inhibitor, but not by bisindolylamaleimide, a protein kinase C (PKC) inhibitor. Herbimycin A treated human or bovine peripheral blood CD4T cells lacked PTK activity and failed to kill virally infected target cells even after cross-linking of CD4molecules. The CD4 cross-linking failed to induce effector cell proliferation or the transcription of TNF$\beta$. Upregulation of TNF$\beta$ was induced by incubating the antibody activated effector cells with BHV-1 infected D17 target cells for 10 h. Anti-TNF$\beta$ antibody partially abolished (13-44%) the direct effector cell-mediated antiviral cytotoxicity. However, this antibody neutralized 70 to 100% of antiviral activity of effector and target cell culture supernatants against BHV-1 infected D17 cells. The inhibition level of the antiviral activity by the antibody was dependent on the effector and target cell ratio. These results support the hypothesis that increased $56^ICK enzyme activity in effector cells transduces a signal critical for effector cell recognition of viral glycoproteins expressed on the target cells. Following target cell recognition, lytic cytokines known to participate in target cell killing were produced. A better understanding of the killing activity displayed by CD$4^+$T lymphocytes following surface receptor cross-linking will provide insight into the mechanisms of cytotoxic activity directed toward virally-infected cells.

• Korean Linguistics
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• v.79
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• pp.145-169
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• 2018

The purpose of this paper is to review critically the validity and relevance of the pan-national language culture improvement movement, Annyeong! Urimal(Hello! Our Language) implemented for the last three years as part of the government-driven projects, and to seek for improvement direction or fundamental reorientation of this movement. In order to accomplish this purpose, this paper firstly points out abstract and conventional aspects of the diagnosis for background and the purpose of projects, and suggests that this movement focuses on verbal abuse rather than language destruction and pays attention to positive aspects of newly rising language use. Then, with reference to main agent, this paper argues that the government must lead actively formation of voluntary nongovernmental agent with indirect financial and institutional support. Thirdly, it is suggested that public language should be the top priority target sphere and the language used by the leaders of society and the older generation should be also included in the target spheres of this movement. Finally, this paper indicates that enlightenment/public relation projects end often in one time events, imposing unilaterally the normative contents, and also emphasizes the importance of survey/research projects and deliberation/evaluation projects.

• The Korean Journal of Pesticide Science
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• v.18 no.3
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• pp.181-190
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• 2014

An entomopathogenic bacterium, Bacillus thuringiensis (Bt), can sporulate along with production of insecticidal Cry toxins. Bt Cry toxins exhibit relatively narrow spectrum to target insects due to their specific interactions with midgut receptors. This study designed several strategies to enhance Bt efficacy in target insect spectrum and insecticidal activity. Four Cry toxins were purified from four different Bt strains and showed relatively narrow target insect spectrum. However, the Cry mixtures significantly expanded their target insect spectra. The additional effect of baculovirus to Cry toxin was tested with recombinant baculoviruses expressing Cry1Ac or Cry1Ca. However, the baculovirus was little effective to expand target insect spectrum. Bacterial culture broth of Xenorhabdus nematophila (Xn) significantly suppressed insect cellular immune response and increased Cry toxicity. The addition of Xn culture broth to Cry mixture significantly enhanced Bt efficacy in target insect spectrum and insecticidal activity.

• The Korean Journal of Archival Studies
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• no.24
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• pp.173-214
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• 2010

Intangible Culture Heritage can show its face only by representation of people in no form. Accordingly, if there are not people who represent Intangible Culture Heritage or records which is about Intangible Culture Heritage, we can see its appearance any more. Now on, Intangible Culture Heritage preservation policy of Korea is incomplete, so polices that Intangible Culture Heritage keeps on its values permanently, are essential. This study starting from these critical mind, suggests documentation method of Gangneung Danoje. This study designs documentation plans of Intangible Culture Heritage considering whore its whole lifecyle that produced, keened, used, preserved. To document the Gangneung Danoje, the target of documentation is selected and divided as ceremony, transmit from generation to generation, related data. the ceremony and transmit from generation to generation of Gangneung Danoje are recognized by building the business process, so records that produces whenever business act produce, subjects who produce the records, the spot where records are produced. A related data is not the target of documentation produced as continual business, but something to worthy of keeping as records is selected. Through investigation of the ceremony, transmit from generation to generation, and related data, this study selects objects to documentation and suggests method of documentation and subjects who document records.

• The Journal of the Korea Contents Association
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• v.17 no.5
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• pp.509-519
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• 2017

This study addresses the effective utilization of film music as learning material for language, target culture, and ethics education. Music is intertwined with language and culture, and even with ethics. This study focuses on the potential power of film music in the processes of teaching and learning in a classroom. For this purpose, five songs are selected from the soundtrack of Disney's famous animation The Lion King: "Circle of life", "I just can't wait to be king", "Be prepared", "Hakuna Matata", and "Can you feel the love tonight?", and concrete learning activities are suggested based on these. Using these five songs, gap-filling and singing-recoding tasks are proposed as listening and speaking activities respectively. Film music is also very useful in learning vocabulary, sentence structure, and grammar. Learners participate in a writing activity involving creating their own lyrics for the tunes reflecting their experiences. Next, for culture education, a teacher asks their students to discuss about, and be aware of, food culture using a specific character's song. Finally, for ethics education, a philosophy of life, natural logic, leadership qualities, and the motto Hakuna Matata("no worries") are explored and discussed through an analysis of the lyrics. The open-ended questionnaire survey is conducted. The result shows that music has a positive effect on culture and ethics education. Film music can be effective in learning a second language, target culture, and ethics.

• The Research Journal of the Costume Culture
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• v.15 no.1 s.66
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• pp.137-151
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• 2007

This study was performed to narrow down the Sizing System for Female Adult's garments(KS K 0051) following age target and body shapes. The study was based on the sizing system and the anthropometric measurements, called Size-Korea, which were surveyed in the years 2003 and 2004. At first, the subjects of the survey were classified into 4 age groups, $18{\sim}24,\;25{\sim}34,\;35{\sim}49\;and\;50{\sim}59$, based on the most significant differences of body sizes. Secondly, the ranges of four key dimensions - height, bust, waist and hip-of normal sizes were identified through analyzing Rohrer index and percentile. The selected sizes within the ranges were grouped by the drop which was the subtraction of bust girth from hip girth. In conclusion, there was the most frequent drop for each age group; 9cm drop for the $18{\sim}24$ years old group, the 6cm drop for the $25{\sim}34$ years old and $35{\sim}49$ years old and 3cm drop for $50{\sim}59$ years old. The upper body garment sizes of the same drop and target ages could be merged to 10 to 12 sizes, which were remarkably smaller than the Female Adult garment sizing system, and further to 2-5 sizes, considering the percentile importance. However, $50{\sim}59$ years old group needed to be analyzed in different ways, because the number of subjects of normal size in the age decreased rapidly, that the normal sizes for them were very limited. In addition, the reference body sizes of each garment size were apparently different when they were compared to each age groups. These basic data for garment size specification could be usefully adapted for the apparel companies' own sizing system according to their age target and body shapes, to achieve efficient production and better fit.