• Korean Institute of Interior Design Journal
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• v.19 no.5
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• pp.95-104
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• 2010

One of 21st century's main paradigms is a 'Culture', and people started to express their 'cultural desires and demands' regarding public environments. Accordingly central and local governments are paying attention to these demands from their citizens and trying to establish the policies to meet these needs. This research is done to suggest the strategy programming model to support the executions of culture-centered public designs which are based on the local resources and identities. The entire research contains three sub-topics. First topic is setting the prototype phases of strategy programming. Second topic is analyzing the associated indices for each prototype phase. Third topic is suggesting Matrix Model in which the prototype phases and their associated indices are linked. Among three topics, this paper deals with the first one - the prototype phases of strategy programming. It studies this subject in relation with Place Marketing which emphasizes the local resources and identities. The prototype phases are comprised of 3 steps for Place Strategy and 4 steps for Marketing Strategy. Place Strategy should be considered prior to Marketing Strategy because in culture-centered public design, locality has priority over other concerns. The phases for Place Strategy includes 'Resource_analyzing of local resources', 'Mission_setting a purpose', and 'Targeting_segmenting target groups'. The phases for Marketing Strategy involves 'Organization_instituting the main body and/or partnership', 'Image Positioning_setting an unique local image', 'Point_realizing the product', and 'Channel_deciding the sales promotion tools'.

• The Korean Journal of Physiology and Pharmacology
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• v.15 no.6
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• pp.431-436
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• 2011

Vascular smooth muscle cells can obtain a proliferative function in environments such as atherosclerosis in vivo or primary culture in vitro. Proliferation of vascular smooth muscle cells is accompanied by changes in ryanodine receptors (RyRs). In several studies, the cytosolic $Ca^{2+}$ response to caffeine is decreased during smooth muscle cell culture. Although caffeine is commonly used to investigate RyR function because it is difficult to measure $Ca^{2+}$ release from the sarcoplasmic reticulum (SR) directly, caffeine has additional off-target effects, including blocking inositol trisphosphate receptors and store-operated $Ca^{2+}$ entry. Using freshly dissociated rat aortic smooth muscle cells (RASMCs) and cultured RASMCs, we sought to provide direct evidence for the operation of RyRs through the $Ca^{2+}$- induced $Ca^{2+}$ -release pathway by directly measuring $Ca^{2+}$ release from SR in permeabilized cells. An additional goal was to elucidate alterations of RyRs that occurred during culture. Perfusion of permeabilized, freshly dissociated RASMCs with $Ca^{2+}$ stimulated $Ca^{2+}$ release from the SR. Caffeine and ryanodine also induced $Ca^{2+}$ release from the SR in dissociated RASMCs. In contrast, ryanodine, caffeine and $Ca^{2+}$ failed to trigger $Ca^{2+}$ release in cultured RASMCs. These results are consistent with results obtained by immunocytochemistry, which showed that RyRs were expressed in dissociated RASMCs, but not in cultured RASMCs. This study is the first to demonstrate $Ca^{2+}$ release from the SR by cytosolic $Ca^{2+}$ elevation in vascular smooth muscle cells, and also supports previous studies on the alterations of RyRs in vascular smooth muscle cells associated with culture.

• Journal of the Korea Fashion and Costume Design Association
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• v.7 no.1
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• pp.101-112
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• 2005

21th is the century of culture. So each country create new culture from tradition, and at the hub, there is Rapid development of network which makes these things to be able. Also, Korea selected Han-Bok as a symbol of Korea culture, and also Han-Bok internationally. Han-Bok expresses our nation's emotion very well, and also, Han-Bok has universality that can stimulate on world's sensitivity. Consequently, this study will arrange and analyze the records of foreigners' feeling who visited in the period of the opening of a port as a target of our study. This study will offer materials for study in the period of the opening of a port. Also, this study will discover the significance and organization of our clothes. Way to wear it and the beauty of our clothes that we didn't even realize or not mentioned on the record because we were too accustomed to our beauty from the ancient time. So, we would like to be basic study that can make our clothes globally and appeal to world's sensitivity by finding the universality. The result of this study is as follows. First, the items of hat made us be called as a 'world of hat' so, Gat, Galmo(갈모), a women's winter hat(조바위), a fur-lined hood(남바위) and Ayam(아얌) will be obtained the world's focus. Secondly, we checked Belt, breast-tie, rainbow striped sleeves and Pocket which append vitality and movement arrested the foreigners' attention. Thirdly, foreigner's were interested with straw shoes and paper shoes.

• Journal of Korean Clinical Nursing Research
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• v.26 no.2
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• pp.186-197
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• 2020

Purpose: The purpose of this study was to develop a program for reinforcing the resilience of new nurses and relation-oriented organizational culture. Methods: An Intervention Research (IR) model was used to develop a program. Literature review, focus group interviews with nurses and need surveys were conducted from August to December 2018. Based on the results of the investigation, the researcher developed the content of a program. The program was revised by nurse managers for the content validation. Results: According to the results of the need surveys, 58.8% of the participants thought relation-oriented culture was the most ideal, and 61.8% of the participants wanted to participate in a program for organizational culture improvement. In the focus group interview, not only new nurses but also wards and nursing organizations should be a target subject of the program. Reinforcement of resilience and relation (3R) program was developed as a one-year course, which includes a 'mentor-mentee' program and a 'thanks' program. Conclusion: It would contribute to improving the resilience of new nurses and creating a relation-oriented organizational culture by 3R program. The 3R program could play a role as expanded program from an existing pragmatic short-term training program for improving the competencies or communication skills of new nurses.

• Journal of Internet of Things and Convergence
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• v.6 no.4
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• pp.73-79
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• 2020

In order to verify the effectiveness of the tea culture treatment program to mitigate the relative aggressiveness, the research will target 10 women aged 70 or older who use the P City-based Senior Citizens' Day Care Center to apply the tea culture treatment program to alleviate the relative aggressiveness of the elderly and examine its effects. As a result of the analysis, the score of reactive relational aggressiveness and leading relational aggression is 0.21 lower than the pre-exam average, indicating that the leading relational aggression of the elderly who participated in the tea culture therapy program is reduced than before the program. Based on these analysis results, practical suggestions were made for alleviating aggression among senior citizens using the Center for the Elderly.

• Journal of Korean Society of Rural Planning
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• v.20 no.1
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• pp.105-113
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• 2014

This study analyzed settlement motivation, life satisfaction factors, policy requirement targeting return farmers on mountain village. 8 target villages where return farmers live at least 10 people were selected according as cooperation of the local governments through literature by considering the area of the whole country. The return farmers in target villages were conducted a face to face survey with village headman's pre-coordination from February to September 2013. A total of 94 surveys were performed and 87 copies were used in the analysis. According to the results, the most important factor influenced the decision to be a return farmer was "my family health(3.97)". The correlation analysis was performed to examine factors related with satisfaction of "return farmer life in general". The result showed that "change of physical health" was the most important correlativity with 0.544 correlation coefficient and 0.05 significant level and "family relationship" was followed with 0.424 correlation coefficient and 0.05 significant level. According to the "important factor for the successful return-farmer life", 42.7% of respondents chose "harmonious relationships with local residents" and "stable source of income" was chosen by 29.3% of residents. A questionnaire for the analysis of policy requirements showed that "education system related Forestry(Agriculture)" was the highest demand with 4.oo points. The result can be used as a basis for establishing policies with activation of mountain villages to attract to constantly increasing population of return farmers.

• KSBB Journal
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• v.30 no.2
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• pp.82-90
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• 2015

Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was 7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.

• Journal of Microbiology and Biotechnology
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• v.20 no.11
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• pp.1534-1538
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• 2010

Fed-batch cultures of Hansenula polymorpha were studied to develop an efficient biosystem to produce recombinant human serum albumin (HSA). To comply with this purpose, we used a high-purity oxygen-supplying strategy to increase the viable cell density in a bioreactor and enhance the production of target protein. A mutant strain, H. polymorpha GOT7, was utilized in this study as a host strain in both 5-l and 30-l scale fermentors. To supply high-purity oxygen into a bioreactor, nearly 100% high-purity oxygen from a commercial bomb or higher than 93% oxygen available in situ from a pressure swing adsorption (PSA) oxygen generator was employed. Under the optimal fermentation of H. polymorpha with highpurity oxygen, the final cell densities and produced HSA concentrations were 24.6 g/l and 5.1 g/l in the 5-l fermentor, and 24.8 g/l and 4.5 g/l in the 30-l fermentor, respectively. These were about 2-10 times higher than those obtained in air-based fed-batch fermentations. The discrepancies between the 5-l and 30-l fermentors with air supply were presumably due to the higher contribution of surface aeration over submerged aeration in the 5-l fermentor. This study, therefore, proved the positive effect of high-purity oxygen in enhancing viable cell density as well as target recombinant protein production in microbial fermentations.

• The Research Journal of the Costume Culture
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• v.26 no.2
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• pp.188-202
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• 2018

This study aimed to categorize women's body shapes by type after extracting prototypes of 25~34 year old Korean woman. The standardizing research service project conducted by the Ministry of Commerce, Industry and Energy in 2005, divided the age ranges of adult women into three groups: 18~24 years (young), 25~34 years (young-adult) and 35~49 years (adult). This study utilized this age division method to create a concrete body type categorization schema with the most marriageable period, 25 to 34 years old as target age the target age group. We used, measurement data from the 7th Korean Human Body Size Survey (Size Korea) for the body shape analysis. We completed a statistical analysis using the statistical program SPSS 21. After creating the body types using CLO 3D, which is based on the 7th Korean human body measurements, we input data for the average size for each type into the Avata. We then compared and analyzed the cross sections using the Rapidform XOR program. The results of the type-specific characteristics are as follows: big square body of obese body, small square body of plain flat body with tall, plain square body of plain flat with short, triangular body of lower body obesity there was. Significantly, the results of this study should facilitate the development of various apparel products using mass customization or easy-order systems.

• Journal of Creative Information Culture
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• v.6 no.3
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• pp.107-128
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• 2020

In this study, the present state and characteristics of VR exhibits currently exhibited in Korea, Hong Kong, and Japan were studied to derive the improvements in the domestic VR exhibits. The previous literatures on VR exhibits at Science Museum were reviewed and online and offline data were collected, analyzed and compared. As the result of the study, VR exhibits at Science Museum in Korea turned out to focus on interest and fun rather than containing scientific content, and exhibits aimed at recreational play and physical activity. The Hong Kong Science Museum VR exhibits are more physically targeted exhibits than the cognitive target exhibits. In addition, at the Japanese Science Museum, there are many exhibits aimed at the physical target exhibits. As the result of classifying VR exhibits into the similar types, it was possible to derive them into four characteristics. The implications for future KOREA VR museum exhibitions were discussed.