• Title/Summary/Keyword: Taq DNA polymerse

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Removal of Contaminating TEM-la $\beta-Lactamase$ Gene from Commercial Taq DNA Polymerase

  • Song Jae Seok;Lee Jung Hun;Lee Jung-Hyun;Jeong Byeong Chul;Lee Won-Keun;Lee Sang Hee
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.126-128
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    • 2006
  • This study confirms that Taq DNA polymerase could be contaminated with the $blaTEM-1_a$ gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.