• Korea Journal of Cosmetic Science
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• v.2 no.1
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• pp.11-19
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• 2020

The aim of this study is to investigate the effect of various pentapeptides on hair repair depending on the characteristics of comprising amino acids using crosslinking agents in hair. Total ten peptides were synthesized with two kinds of amino acids respectively, of which were previously categorized according to R group of the amino acids contributing to the characteristic of each peptide: STTSS (Ser-Thr-Thr-Ser-Ser), LIILL (Leu-Ile-Ile-Leu-Leu), CMMCC (Cys-Met-Met-Cys-Cys), DEEDD (Asp-Glu-Glu-Asp-Asp), RKKRR (Arg-Lys-Lys-Arg-Arg), TAMRA-STTSS, TAMRA-LIILL, TAMRA-CMMCC, TAMRA-DEEDD, and TAMRA-RKKRR. Pentapeptide alone, or pentapeptides with crosslinking agents such as polymeric carbodiimide (PCI) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were treated to chemically damaged hair. Hair diameter and break strength (N = 40/case) were measured to calculate tensile strength of hair for computing hair repair ratio, and fluorescence yields (N = 20/case) were collected for hair treated with TAMRA-peptides. The tensile strength of hair treated with pentapeptides alone, or pentapeptides with cross-linking agents is consistent with the fluorescence yield from the microscope images of the cross-sectioned hair in vision and in numerical values. Pentapeptides consisting of hydrophobic amino acids (LIILL), amino acids with sulfur (CMMCC), and basic amino acids (RKKRR) increased the tensile strength in perm-damaged hair. Pentapeptides with no extra carboxyl/amine groups in R group of amino acids resulted in no significant differences in hair strength and fluorescence yield among hairs treated with alone and with crosslinkers. Pentapeptides with extra carboxyl groups or amine groups enabled further strengthening of hair due to increased bonds within the hair after carbodiimide coupling reaction. The hair repairs of pentapeptides with various amino acid sequences were studied using crosslinking. Depending on the physical characteristics of comprising amino acids, the restoration of damaged hair was observed with tensile strength of hair and fluorescence signals upon cross-sectioned hair in parallel to possibly understand the binding tendency of each pentapeptide within the hair.

• Korean Journal of Veterinary Research
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• v.54 no.1
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• pp.13-19
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• 2014

Animal bones excavated with earthenware from the shell mound at the Jeju Jongdali 1819 archeological site, where three consecutive chronological layers covering the Neolithic (B.C. 15C-B.C. 10C), early Tamra, and late Tamra periods have been identified, were morphologically classified. The majority of the bones from all three periods were broken or split. The major fauna of the mammalian bones in all periods were Cervus spp., Sus scrofa, and Bos taurus. In the early and late Tamra periods, bones of small animals including Mustela sibirica coreana, Meles meles, Rodentia, and Aves were also found in small number. The excavated bones were from all parts of the animal bodies, including head, trunk, forelimb, and hindlimb. Collectively, these findings suggest that the major fauna from the Neolithic to late Tamra periods consisted of Cervus spp., Sus scrofa, and Bos taurus and that the fauna was dissected and carried to the shell mound site after hunting. Information from the bone remains in the shell mound are useful data for study of the wildlife and domestic animals living during the prehistoric period of Jeju Island.

• Journal of The Geomorphological Association of Korea
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• v.21 no.4
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• pp.149-164
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• 2014

The representative landscapes in Jeju Island called as Ten sceneries of Yeongju originates with Ten sceneries of Tamra which were organized in the late seventeenth century by Jeju governor Lee Iktae. In order to compose ten representative sceneries in Jeju Island, he selected four strongholds such as Jocheon, Byeolbang, Seogwi and Myeongwol and six places of scenic beauty such as Seongsan, Baekrokdam, Yeonggok, Cheonjiyeon, Sanbang and Chwibyeongdam, and made a painting named as Ten sceneries of Tamra. Since the scenes of strongholds also exhibit a lot of geomorphic landscapes, the painting could be considered a collection of representative landforms in Jeju Island. The painting depicts several types of landform such as volcano, crater, river, waterfall, pool, wetland, cavern, tor and rocky coast. The volcanic edifice appears most frequently in the painting, reflecting the particular emotion of Jeju people on Mt. Halla and oreums. However, another volcanic landform, a lava tube, was not highly evaluated due to its negative images. Three famous waterfalls in Jeju Island were included in Ten sceneries of Tamra, and it suggests that they are essentially outstanding landforms, and besides, there are few sites related with fresh water in Jeju Island. The ten sceneries were entirely organized in spite of the limitation of selecting places at that time. The landscape images of Jeju Island, which had been shared as collective representation by Jeju people, were firstly externalized through Lee Iktae's painting, and have long been passed down and established a kind of scenic stereotype.

• The Journal of the Convergence on Culture Technology
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• v.9 no.4
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• pp.127-134
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• 2023

The god of water, Baltara (Bhadra: in the northern part of India), Tammola Around 563-483, he came to Tamra with 900 Arhats (BC483-AD1105, during the reign of King Sukjong of Goryeo, the kingdom of Tamra became Hangun-hyeon in Goryeo). It is the spread of Buddhism through the world's most sacred water (Heiligkeit, one of the four elements water, fire, air, and earth) and the world's largest Arhat The reverse of Halla, appendix: to find the stone statue of Buddha at Seogo Temple in Jeonju, Hwangbangsan. Since ancient times, Jeju people have called Truth Mt. that Mt. Halla protects against typhoons blowing from the south. It looks like it has been hardened by fire. On the coming day, the greatest breath of Venerable Baltara in Jeju is Olle Road No. 1 from Siheung-ri, Seongsanpo to No. 26 Gujwa-myeon Jongdal-ri. It is the Oval Jeju "Road Way" that clearly shows "the beginning is the end, and the end is the beginning" of Cheonbugyeong.

• Korean Journal of Microbiology
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• v.46 no.1
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• pp.96-103
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• 2010

Oligosaccharyltransferase (OTase) catalyzes the transfer of a lipid-linked oligosaccharide (LLO) to the nascent polypeptide. Most eukaryotes have an OTase composed of a multisubunit protein complex. However, the kinetoplastid Leishmania major and the bacterium Campylobacter jejuni have only a single subunit for OTase activity, Stt3p and PglB, respectively. In this study, a new in vitro assay for OTase was developed by using a fluorescent peptide containing N-glycosylation sequon, Asn-Xaa-Thr/Ser, where Xaa can be any amino acid residue except Pro. L. major Stt3p and C. jejuni PglB as a model OTase enzyme demonstrated the formation of glycopeptides from a fluorescent peptide through OTase activities. For separation and measurement of the glycopeptides produced by the OTases, Tricine-SDS-PAGE, a lectin column and fluorospectrophotometer, and HPLC were applied. Comparison of these assay methods for analyzing a fluorescent glycopeptide showed HPLC analysis is the best method for separation of glycopeptides and nonglycosylated peptides as well as for quantify the peptides than other methods.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.2
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• pp.101-108
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• 2012

To determine sulfate uptake and assimilation of various rape cultivars (Brassica napus L.) under different S levels, the activity of ATP sulfurylase, ${SO_4}^{2-}$ uptake and glutathione (GSH) concentrations were measured in different age of leaves. In this study, ten rape cultivars (Mokpo, Tamra, Youngsan, Naehan, Saturnin, Akela, Mosa, Capitol, Pollen, and Colosse) showed various sulfate uptake and assimilation capacity in response to inefficient sulfate supply. Under inadequate sulfate conditions, a greater ATP sulfurylase activity in young leaves was observed in all cultivars compared to that of old leaves. In addition, GSH concentration was considerably increased in young leaves when S supply was declined from 2.0 to 0.2 mM. These results suggested that rape cultivars differ in their capacity to utilize sulfate under limited S conditions.

• Korean Journal of Environmental Agriculture
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• v.36 no.1
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• pp.7-16
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• 2017

BACKGROUND: Increased value added by rapeseed (Brassica napus) by-product and the development of a usable rapeseed functional tea. METHODS AND RESULTS: To develop a usable rapeseed functional tea, the total flavonoid content in the varieties Youngsan, Tammi, Tamra, Naehan, Hanra, Mokpo No. 68, and Mokpo No. 111 was investigated. Effect of three treatments, i.e., drying, leaching, and roasting, on flavonoid contents or flower was tested using multiple processing methods per treatment. Total flavonoid content decreased under the various drying methods, confirming that flavonoid content is heat-dependent. This finding was more pronounced for freezing and oven-drying (15.3 and 13.8 mg/g DW, respectively), with a 10% difference in the total flavonoid content between the two methods. Under leaching conditions, the flavonoid content decreased with increasing treatment time. Notably, roasting methods did not result in loss of flavonoid content. The total flavonoid content in the rapeseed varieties decreased in the following order: Youngsan, Tammi, Tamra, Naehan, Hanra, Mokpo No. 68, and Mokpo No. 111. CONCLUSION: The flavonoid content in rapeseed flower was higher in Youngsan than in the other varieties, under processing conditions such as freeze-drying, leaching at $90^{\circ}C$ for 5 min, and roasting.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.2
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• pp.71-74
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• 2008

To investigate the sulfate utilization efficiency in different rape (Brassica napus) cultivars, sulfate uptake are analyzed under complete S-supply level (2.0mM ${SO_4}^{2-}$). This study used ten different genotypes of rape (Mokpo, Tamra, Youngsan, Naehan, Saturnin, Akela, Mosa, Capitol, Pollen and Colosse). For comparison of ${SO_4}^{2-}$ uptake among 10 cultivars, leaf number, leaf length and width, root length was also observed. Leaf length and width in all cultivars less variable among the cultivars examined. The longest root was shown in Saturnin (36.3 cm). ${SO_4}^{2-}$ uptake in Saturnin, Youngsan and Mokpo was significantly higher whereas that of Mosa and Pollen was relativety lower. Saturnin and Mokpo which have a high ${SO_4}^{2-}$ uptake exhibited a high ${NO_3}^-$ uptake.

• Journal of Microbiology and Biotechnology
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• v.24 no.9
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• pp.1291-1299
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• 2014

Recently, multifunctional nanomaterials have been developed as nanotherapeutic agents for cellular imaging and targeted cancer treatment because of their ease of synthesis and low cytotoxicity. In this study, we developed a multifunctional, two-component nanorod consisting of gold (Au) and nickel (Ni) blocks that enables dual-fluorescence imaging and the targeted delivery of small interfering RNA (siRNA) to improve cancer treatment. Fluorescein isothiocyanate-labeled luteinizing hormone-releasing hormone (LHRH) peptides were attached to the surface of a Ni block via a histidine-tagged LHRH interaction to specifically bind to a breast cancer cell line, MCF-7. The Au block was modified with TAMRA-labeled thiolated siRNA in order to knock down the vascular endothelial growth factor protein to inhibit cancer growth. These two-component nanorods actively targeted and internalized into MCF-7 cells to induce apoptosis through RNA interference. This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment, with capabilities in dual imaging and targeted gene delivery.

• Korean Journal of Veterinary Research
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• v.36 no.4
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• pp.757-761
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• 1996

The animal bone-remains excavated with earthwares at Cheju Kimnyungri cave site were investigated morphologically and osteometrically. The number of bone remains were 1706 pieces by morphological criteria. Based on the classification of bony pieces excavated in the cave site, the important animal species in Cheju island during the early Tamra period (presumably between A.D.0 - A.D. 500) was as follows; wild boar(75%), deer(17%), cattle(6%), and horse in small percentage. The excavated bone remains imply that the major fauna of animal species are composed of three species, including Sus scrofa, Cervus nippon and Bos taurus. These data suggests that the archaeological remains such as bone pieces are good indicators of the fauna animals, and of zoological entity in the island.