• Parasites, Hosts and Diseases
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• 제57권4호
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• pp.417-422
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• 2019

From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

• Parasites, Hosts and Diseases
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• 제52권1호
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• pp.79-83
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• 2014

Human taeniases had been not uncommon in the Republic of Korea (=Korea) until the 1980s. The prevalence decreased and a national survey in 2004 revealed no Taenia egg positive cases. However, a subsequent national survey in 2012 showed 0.04% (10 cases) prevalence of Taenia spp. eggs suggesting its resurgence in Korea. We recently encountered 4 cases of Taenia saginata infection who had symptoms of taeniasis that included discharge of proglottids. We obtained several proglottids from each case. Because the morphological features of T. saginata are almost indistinguishable from those of Taenia asiatica, molecular analyses using the PCR-RFLP and DNA sequencing of the cytochrome c oxidase subunit 1 (cox1) were performed to identify the species. The PCR-RFLP patterns of all of the 4 specimens were consistent with T. saginata, and the cox1 gene sequence showed 99.8-100% identity with that of T. saginata reported previously from Korea, Japan, China, and Cambodia. All of the 4 patients had the history of travel abroad but its relation with contracting taeniasis was unclear. Our findings may suggest resurgence of T. saginata infection among people in Korea.

• Parasites, Hosts and Diseases
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• 제57권3호
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• pp.313-318
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• 2019

In recent years, the taeniasis has been rarely reported in the Republic of Korea (Korea). But in this study, we intend to report 4 taeniasis cases caused by Taenia saginata during a 5-month period (February to June 2018) at a unversity hospital in Gwangju, Korea. Worm samples (proglottids) discharged from all cases were identified by phenotypic and molecular diagnostics. Mitochondrial cytochrome c oxidase subunit I sequences showed 99.4-99.9% identity with T. saginata but, differed by 4% from T. asiatica and by 7% from T. multiceps, respectively. We found that tapeworms in 2 cases (Cases 2 and 3) yielded exactly the same sequences between them, which differed from those in Cases 1 and 4, suggesting intra-species variation in tapeworms. These taeniasis cases by T. saginata infection in this study, which occurred within a limited time period and region, suggest the possibility of a mini-outbreak. This study highlights the need for further epidemiological investigation of potentially overlooked cases of T. saginata infection in Korea.

• 생명과학회지
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• 제20권6호
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• pp.955-959
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• 2010

아시아조충과 무구조충의 편절은 형태학적으로 유사해 감별진단하기가 쉽지 않다. 하지만 아시아조충의 경우 감염자에서 낭미충증 등의 심각한 합병증을 일으킬 가능성을 배제할 수 없으므로 두 기생충의 정확한 진단이 필요하다. 최근 기생충학 분야에서 DNA 서열에 기초한 진단 방법이 널리 쓰이고 있다. 본 연구에서는 다중 중합 효소연쇄반응을 이용해 한국인에서 발견된 태니아 속 조충류의 감별진단을 시도해 보고자 했다. 중합효소연쇄반응을 위해 Ta4978F, Ts5058F, Tso7421F, Rev7915 4개의 시발체(프라이머)를 사용했으며, 그 결과 태니아의 종 동정이 정확하고 신속하게 이루어질 수 있었다. 중합효소연쇄반응 방법을 도입한다면 한국에서 인체 태니아 조충의 역학적 소견을 용이하게 재검토할 수 있으리라고 생각한다.