• Molecules and Cells
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• v.19 no.2
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• pp.185-190
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• 2005

The chicken oviduct is a dynamic organ that produces secretory proteins such as ovalbumin and its cells undergo cell proliferation and differentiation. There has been no study of the cellular mechanism involved in cell death in the chicken oviduct. Therefore, this study has focused on the study of apoptosis in primary oviduct cells. Because ceramide is known to activate apoptosis in tumor cells and is produced in the oviduct, we used an exogenous ceramide analog to induce cell death. The viability of ceramide-treated chicken oviduct cells decreased in a dose-dependent manner and apoptotic cells were detected by staining with annexin V. The expression of apoptosis-related genes was assessed by RT-PCR and bcl-2 mRNA was found to decrease after exposure to ceramide while Bcl-x mRNA increased 12 h post-treatment. In addition, caspase-3 was expressed strongly in the early stages of apoptosis, while caspase-1 and -9 transcripts increased at later times. We conclude that ceramide induces apoptosis in oviduct-derived primary cells via a caspase- and bcl-2-dependent pathway.

• Molecules and Cells
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• v.27 no.6
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• pp.641-649
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• 2009

Pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) catalyzes the reversible interconversion of fructose-6-phosphate and fructose-1,6-bisphosphate, a key step in the regulation of the metabolic flux toward glycolysis or gluconeogenesis. To examine the role of PFP in plant growth, we have generated transgenic Arabidopsis plants that either overexpress or repress Arabidopsis PFP subunit genes. The overexpressing lines displayed increased PFP activity and slightly faster growth relative to wild type plants, although their photosynthetic activities and the levels of metabolites appeared not to have significantly changed. In contrast, the RNAi lines showed significantly retarded growth in parallel with the reduced PFP activity. Analysis of photosynthetic activity revealed that the growth retardation phenotype of the RNAi lines was accompanied by the reduced rates of $CO_2$ assimilation. Microarray analysis of our transgenic plants further revealed that the altered expression of $AtPFP{\beta}$ affects the expression of several genes involved in diverse physiological processes. Our current data thus suggest that PFP is important in carbohydrate metabolism and other cellular processes.

• Journal of Broadcast Engineering
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• v.19 no.6
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• pp.847-853
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• 2014

When a disaster occurs, effective alerts will reduce human life and property loss. The Integrated Emergency Alert System is a national disaster management system which integrates existing alert systems to deliver effective disaster alerts. Alert message exchange protocol is the essential technology for the System. In Korea, the first specification for "Common Alerting Protocol Profile for Integrated Emergency Alert System" was established in 2014. In this paper, we derive two additional requirements for more enhanced alert service and propose revision of the specification: multilingual alert service for foreigners and more flexible representation of target area. We review the associated foreign profile specifications as well as the domestic specifications to analyze these requirements. Finally, we propose the most efficient way to deliver multilingual alert message and to represent target area.

• Journal of Acupuncture Research
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• v.27 no.6
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• pp.101-109
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• 2010

Objectives : This study was to compare effects of scalp acupuncture with usual acupuncture on peripheral facial palsy in comparison with usual acupuncture only. Methods : We investigated 40 cases of patients with peripheral facial palsy, and devided patients into two groups: We treated one group by scalp acupuncture with usual acupuncture and did the other group by usual acupuncture only. To evaluate the effectiveness of treatment applied for two groups, we used Gross Grading System of House-Brackmann(H-B grade), Yanagihara's Unweighed Grading System(Y system). Results : 1. In H-B grade group B(common acupuncture therapy and scalp acupuncture)'s score was reduced than group A(common acupuncture therapy), but there was no statistical significance. 2. In Y-system group B was higher than group A, but there was no statistical significance. Conclusions : There was no stastical significance between two groups : treated by scalp acupuncture with usual acupuncture and usual acupuncture therapy only, on peripheral facial palsy.

• Journal of Korean Foot and Ankle Society
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• v.8 no.2
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• pp.176-181
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• 2004

Purpose: We evaluated the clinical results of operative treatment of the intraarticular calcaneal fracture according to fracture classification. Materials and Methods: There were 25 cases (24 patients) which had at least one year follow up, 17 men and 7 women who were treated from June 1997 to March 2003. We have analysed the Bohler's angle, cruciate angle, width and posttraumatic osteoarthritis in radiological evaluation, and evaluated clinical results according to the Creighton-Nebraska health foundation score. Results: Excellent results were noted in 7 cases, good results in 6 cases, fair results in 4 cases and poor results in 8 cases. Radiological changes showed as follows: Bohler angle $17^{\circ}$, Crucial angle $0.1^{\circ}$, Width 6mm. Conclusion: Type II showed satisfactory results and type IV showed unsatisfactory results in Sanders classification. Proper evaluation of the intraarticular calcaneal fracture by X-ray and CT scan is necessory to predict prognosis and decise method of operative treatment.

• Transactions on Electrical and Electronic Materials
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• v.15 no.4
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• pp.198-200
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• 2014

100 nm thick Ga doped ZnO (GZO) thin films were deposited with RF magnetron sputtering on polyethylene terephthalate (PET) and ZnO coated PET substrate and then the effect of the ZnO thickness on the optical and electrical properties of the GZO films was investigated. GZO single layer films had an optical transmittance of 83.7% in the visible wavelength region and a sheet resistance of $2.41{\Omega}/{\square}$, while the optical and electrical properties of the GZO/ZnO bi-layered films were influenced by the thickness of the ZnO buffer layer. GZO films with a 20 nm thick ZnO buffer layer showed a lower sheet resistance of $1.45{\Omega}/{\square}$ and an optical transmittance of 85.9%. As the thickness of ZnO buffer layer in GZO/ZnO bi-layered films increased, both the conductivity and optical transmittance in the visible wavelength region were increased. Based on the figure of merit (FOM), it can be concluded that the ZnO buffer layer effectively increases the optical and electrical performance of GZO films as a transparent and conducting electrode without intentional substrate heating or a post deposition annealing process.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.2
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• pp.167-174
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• 2018

The heater is applied to the lunar lander for securing its survivability under severe lunar thermal environment during 14 days of night time. For this, the heater on/off set-points shall be determined to minimize the power consumption due to the limited power generation of lunar lander during night time. In addition, the temperature changes of the lander according to the heater set-point is also an important factor because it is related to thermo-mechanical reliability on solder joint of on-board electronics. In this study, we investigated thermo-mechanical reliability on solder joint according to the heater set-point by using commercial reliability and a life prediction tool of Sherlock based on the thermal analysis results of lunar lander that is a year of the mission lifetime.

• The Journal of Internal Korean Medicine
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• v.31 no.3
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• pp.554-568
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• 2010

Objective : This study was examined to investigate the effect of Gamitongseong-san(GTS) extract on spontaneous hypertension. Methods : After administering GTS extract to SHR for 4 weeks, we measured anti-oxygen effects, weight of body, heart and kidney, blood pressure, heart rate, aldosterone, catecholamine, electrolyte, uric acid, and BUN. Results : 1. GTS increased 2,2-diphenyl-1-picrylhydrazyl(DPPH) scavenging activity and superoxide dismutase(SOD) similar activity depending on the concentration. 2. GTS significantly decreased heart weight in spontaneously hypertensive rat. 3. GTS significantly decreased blood pressure and heart rate in SHR. 4. GTS significantly decreased aldosterone. 5. GTS significantly decreased norepinephrine and epinephrine. 6. GTS significantly decreased $K^+$. 7. GTS significantly decreased BUN. Conclusions : These results suggest that GTS may be usefully applied for the treatment of hypertension.

• Journal of Sensor Science and Technology
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• v.28 no.6
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• pp.366-370
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• 2019

In this study, we have successfully fabricated a highly conductive transparent electrode using Ag nanowires, based on piezoelectric polyvinylidene difluoride (PVDF) film, that can be applied as transparent and flexible speakers. The structural morphology of the Ag nanowires was confirmed by a detailed scanning electron microscopy. Ultraviolet-visible spectroscopy demonstrated that the transparent electrode fabricated by the Ag nanowires exhibited a transmittance of above 70%. The transparent electrode also showed very low sheet resistance with high flexibility. We have further developed an anti-oxidation coating layer by using a tetraethyl orthosilicate-poly trimethyloxyphenylsilane (TEOS-PTMS) slurry technique. It was confirmed that the transmittance and sheet resistance of the antioxidant film depends critically on the humidity of the film surface. We believe such Ag nanowire electrodes are a very promising next-generation transparent electrode technology that can be used in future flexible and transparent devices.

• Journal of Applied Biological Chemistry
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• v.51 no.2
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• pp.45-49
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• 2008

Cytosolic fructose-1,6-bisphosphatase (cFBPase) in plants is a key regulatory enzyme in the photosynthetic sucrose biosynthesis. Plant cFBPases, like the mammalian FBPases, are inhibited by adenosine 5'-monophosphate (AMP) and fructose-2,6-bisphosphate (Fru-2,6-$P_2$). In the mammalian FBPases, Lys112 and Tyr113 play important roles in the AMP binding. To understand roles of the corresponding residues, Lys115 and Tyr116, in pea cFBPase, the mutant cFBPases were generated by site-directed mutagenesis. The alterations of Lys115 to Gin and Tyr116 to Phe displayed small changes in $K_m$ and $K_i$ for Fru-2,6-$P_2$, indicating that the mutation causes minor effects on the enzyme catalysis and Fru-2,6-$P_2$ binding, whereas resulted in higher than 500-fold increase of $[AMP]_{0.5}$ compared with that of the wild-type enzyme. Results indicate the residues Lys115 and Tyr116 play important roles in the binding of AMP to the allosteric site of the pea cFBPase.