• 대한토목학회논문집
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• 제26권1A호
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• pp.99-106
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• 2006

일축대칭의 단면특성을 갖는 T형 부재는 단면의 특성상 축방향 압축력으로 인하여 전체좌굴이 발생할 경우 휨-비틀림 좌굴이 지배모드가 된다. 인발성형 T형 부재의 휨-비틀림 좌굴거동을 실험적 연구를 통하여 알아보았다. E-glass/vinylester와 E-glass/polyester로 만들어진 2종류의 인발성형 부재가 사용되었으며, 보강층의 배치, 보강층의 두께, 구성물질의 부피비, 역학적 성질 등을 실험적으로 규명하였다. 좌굴실험에서 휨 및 비틀림에 대한 단순지지 조건을 만족시키기 위해서 knife edge를 사용하였으며, 3개의 potentiometer를 사용하여 실험체의 횡변위와 비틀림각을 측정하였다. 모든 실험체에 휨-비틀림 좌굴이 발행하였으며, 대부분의 실험체가 후좌굴 강도를 가지고 있음을 알 수 있었다.

• Journal of Photoscience
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• 제9권2호
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• pp.51-54
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• 2002

We propose a novel mechanism (Twist Sharing Mechanism) for the cis-trans photoisomerization of rhodopsin, based on the molecular dynamics (MD) simulation study. New things devised in our simulations are (1) the adoption of Mt. Fuji potentials in the excited state for twisting of the three bonds C9=C10, C11=C12 and C13=14 which are modeled using the detailed ab initio quantum chemical calculations and (2) to use the rhodopsin structure which was resolved recently by the X-ray crystallographic study. As a result, we found the followings: Due to the intramolecular steric hindrance between 20-methyl and 10-H in the retinal chromophore, the C12-C13 and C10-C11 bonds are considerably twisted counterclockwise in rhodopsin, allowing only counterclockwise rotation of the C11 =C12 in the excited state. The movement of 19-methyl in rhodopsin is blocked by the surrounding three amino acids, Thr 118, Met 207 and Tyr 268, prohibiting the rotation of C9=C10. As a result only all-trans form of the chromophore is obtainable as a photoproduct. At the 90$^{\circ}$ twisting of C11=C12 in the course of photoisomerization, twisting energies of the other bonds amount to about 20 kcal/mol. If the transition state for the thermal isomerization is assumed to be similar to this structure, the activation energy for the thermal isomerization around C11=C12'in rhodopsin is elevated by about 20 kcal/mol and the thermal isomerization rate is decelerated by 10$\^$-14/ times than that of the retinal chromophore in solution, protecting photosignal from the thermal noise.

• 인문언어
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• 제2권2호
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• pp.5-50
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• 2002

This paper aims to illustrate and illuminate the relationship between language and its neighbor disciplines, in particular between language and literature, language and religion, and language and music. 1. Language and literature. Literature is an art of language. Therefore, linguistics, the science of language, should be able to explain how the grammar of literature elevates and ordinary language into a literary language. I illustrate poetic syntax with examples from Shelley, Coleridge, and Wordsworth. 2. Language and religion. I show how a linguistic analysis of a religious text can illuminate the background, authorship, chronology, etc., of a religious text with an example from the Book of Daniel. I also illustrate how a misanalysis of a poetic meter led to a mistranslation with an example from the Book of Psalms. 3. Language and music. First I trace an epochal event in the history of the Western music, i.e., the change of the musical style from the liturgical music of Latin in which the rhythm was created by the alternation of syllable duration into the liberated music of German in which the rhythm was generated by the alternation of lexical stress. I then illustrate a parallelism between linguistic and musical structures with several musical pieces including Gregorian chant, the 16th century music of Palestrina, the 17th century music of Schutz, the 18th century music of Mozart, and the 19th century Viennese music. Finally, the importance of text-tune (verse-melody) association is discussed with examples of mismatches in translated Korean hymns and contemporary Korean lyrical songs. In the concluding part, I speculate on some factors that are responsible for the same organizational devices in three different modes of human communication. An answer may be that all are under the same laws of mind that govern the way man perceives and organizes nature, i.e., the same cognitive abilities of man, in particular, the capacity to organize and impose structure on their respective inputs.

• 천문학회보
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• 제40권1호
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• pp.66.3-67
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• 2015

Without scrutinizing reflection, the plasma comprising a coronal loop is usually regarded to reside within a flux rope. This picture seems to have been adopted from laboratory plasma pinches, in which a plasma of high density and pressure is confined in the vicinity of the flux rope axis by magnetic tension and magnetic pressure of the concave inward magnetic field. Such a configuration, in which the plasma pressure gradient and the field line curvature vector are almost parallel, however, is known to be vulnerable to ballooning instabilities (to which belong interchange instabilities as a subset). In coronal loops, however, ideal MHD (magnetohydrodynamic) ballooning instabilities are impeded by a very small field line curvature and the line-tying condition. We, therefore, focus on non-ideal (resistive) effects in this study. The footpoints of coronal loops are constantly under random motions of convective scales, which twist individual loop strands quite randomly. The loop strands with the axial current of the same direction tend to coalesce by magnetic reconnection. In this reconnection process, the plasma in the loop system is redistributed in such a way that a smaller potential energy of the system is attained. We have performed numerical MHD simulations to investigate the plasma redistribution in coalescence of many small flux ropes. Our results clearly show that the redistributed plasma is more accumulated between flux ropes rather than near the magnetic axes of flux ropes. The Joule heating, however, creates a different temperature distribution than the density distribution. Our study may give a hint of which part of magnetic field we are looking to in an observation.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4271-4274
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• 2014

The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance and metastasis in diverse solid tumors. We previously reported that 48 h treatment of anti-cancer drug doxorubicin could induce EMT in human gastric cancer BGC-823 cells. However, the long term effects of this transient drug treatment were unknown. In this study we found that after 48 h treatment with $0.1{\mu}g/ml$ doxorubicin, most cells died during next week, while a minor population of cells survived and formed colonies. We propagated the surviving cells in drug free medium and found that these long term cultured drug survival cells (abbreviated as ltDSCs) retained a mesenchymal-like cell morphology, and expressed high levels of EMT-related molecules such as vimentin, twist and ${\beta}$-catenin. The expression of chromatin reprogramming factors, Oct4 and c-myc, were also higher in ltDSCs than parental cells. We further demonstrated that the protein level of p300 was upregulated in ltDSCs, and inhibition of p300 by siRNA suppressed the expression of vimentin. Moreover, the ltDSCs had higher colony forming ability and were more drug resistant when compared to parental cells. Our results suggested that an epigenetic mechanism is involved in the EMT of ltDSCs.

• International Journal of Oral Biology
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• 제43권2호
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• pp.69-76
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• 2018

Oral squamous cell carcinoma (OSCC) is the most common type of oral malignancy. Numerous therapies have been proposed for its cure. Research is continually being conducted to develop new forms of treatment as current therapies are associated with numerous side-effects. Luteolin, a common dietary flavonoid, has been demonstrated to possess strong anti-cancer activity against various human cancer cell lines. Nevertheless, research into luteolin-based anticancer activity against oral cancer remains scarce. Thus, the objective of this study was to assess the effect of luteolin as an anti-cancer agent. After treatment with luteolin, Ca9-22 and CAL-27 oral cancer cells showed condensed nuclei and enhanced apoptotic rate with evidence of mitochondria-mediated apoptosis. Epithelialmesenchymal transition (EMT) is closely related to tumor migration and invasion. Luteolin suppressed cancer cell invasion and migration in the current study. Elevated expression of E-cadherin, an adherens junction protein, was evident in both cell lines after luteolin treatment. Luteolin also significantly inhibited transcription factors (i.e., N-cadherin, Slug, Snail, Twist, and ZEB-1) that regulated expression of tumor suppressors such as E-cadherin based on Western blot analysis and quantitative PCR. Thus, luteolin could induce mitochondrial apoptosis and inhibit cancer cell invasion and migration by suppressing EMT-induced transcription factors.

• Toxicological Research
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• 제31권2호
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• pp.151-156
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• 2015

5-Fluorouracil (5-FU) is commonly used for the therapy of colon cancer; however, acquired resistance to 5-FU is a critical barrier to successful treatment and the primary cause of chemotherapy failure. Epithelial-mesenchymal transition (EMT) is a process whereby cells undergo alterations in morphology and molecular characteristics promoting tumor progression and metastasis. Accumulating evidence shows that transition from epithelial to mesenchymal phenotype in cancer cells is associated with their resistance to chemotherapy. However, it is still poorly understood whether EMT is involved in acquired resistance to 5-FU. In this study, we developed an in vitro cell model, 5-FU-resistant HT-29 colon cancer cells, and characterized the differences in cellular morphology and molecular alterations between parental and resistant cells. In accord with mesenchymal-like morphology of 5-FU-resistant HT-29 cells, the expression of the mesenchymal marker fibronectin was significantly increased in these cells in comparision with that in the parental cells. Of interest, we also found a marked increase in the expression of EMT-inducing transcription factors Twist, Zeb1, and Zeb2. Finally, 5-FU-resistant cells showed enhanced migration in comparison with parental HT-29. Taken together, these results indicate that EMT could be associated with 5-FU resistance acquired by HT-29 cells. A specific role of each transcription factor found in this study will require further investigation.

• Bulletin of the Korean Chemical Society
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• 제27권4호
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• pp.568-572
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• 2006

To study the effect of sequence on DNA structure, the two decamer crystal structures one alternating,d(GTACGCGTAC), and the other non-alternating, d(GGCCGCGGCC), were solved. Crystals of both decamers belong to the hexagonal space group $P6_122$, with one strand in the asymmetric unit. The unit cell constants of the alternating decamer are a = b = 39.26 $\AA$, c = 77.70 $\AA$. The structure was refined with 1,828 reflections from 8.0 to 2.0 Aresolution to an R value of 21.3% with all DNA atoms and 63 water molecules. The isomorphous non-alternating decamer had unit cell dimensions of a = b = 39.05 $\AA$, c = 82.15 $\AA$. The structure was refined with 2,423 reflections from 8.0 to 2.0 $\AA$ resolution to a final R value of 22.2% for all DNA atoms and 65 water molecules. Although the average helical parameters of the decamers are typical of A-DNAs, there are some minor differences between them. The helical twist, rise, x-displacement, inclination and roll alternate in the alternating decamer, but do not in the non-alternating decamer. The backbone conformations in both structures show some differences; the residue G(7) of the alternating decamer is trans for $\alpha$ and $\gamma$ while the trans conformations are observed at the residue G(8) of the non-alternating decamer.

• 만화애니메이션 연구
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• 통권36호
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• pp.1-17
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• 2014

이 연구는 형식적인 측면으로 쇼트에서의 아이러니적 반전과 내용적인 측면으로 내러티브 구조 안에서 복선으로 인한 아이러니를 살펴보고자 하였다. 미국의 주요 애니메이션 제작사인 PDI Dreamworks, Pixar에서 만들어진 최근 장편 애니메이션에서 아이러니가 어떻게 사용되었는지 고찰해보고자 하였다. 표현된 것과 숨겨진 실제 간의 괴리인 아이러니는 본 연구에서 살펴본 바와 같이 두 가지로 정리된다. 아이러니는 상황설정 후 예상과 암시의 과정을 거쳐 마지막 장면에서 극적으로 나타난다. 주로 극중에 등장하는 캐릭터의 성격묘사나 사건이 일어날 배경의 설정을 위해 사용되어 웃음을 전해준다. 두 번째로 아이러니와 관련하여 애니메이션 초반에 등장한 복선이 극의 중반 또는 후반에 인과관계를 가지고 반전으로 드러났다. 이 아이러니는 극적인 요소를 강화시키며 이야기를 풍성하게 만들어주기 위해 사용되었다. 미국의 주요 제작사에서 제작된 두 편의 장편 애니메이션에서 분석해본 결과, 연속되는 장면에서의 아이러니나 내러티브 안에서 연속되는 등의 다양한 아이러니적 표현의 사용을 찾아볼 수 있었다.

• Biomolecules & Therapeutics
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• 제28권5호
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• pp.405-413
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• 2020

Fibroblast-like synoviocytes (FLS) play a crucial role in initiating rheumatoid arthritis. B-cell activating factor (BAFF) plays a role in FLS survival as well as in B cell maturation and maintenance. Here, we investigated whether tumor necrosis factor (TNF)-α-induced BAFF expression controls FLS migration and whether BAFF expression in FLS could be regulated by KR33426 which is the inhibitor of BAFF binding to BAFF receptors (BAFF-R) by using MH7A synovial cells transfected with the SV40 T antigen. More TNF-α-treated cells migrated compared to the control. TNF-α increased BAFF expression in FLS, significantly. FLS migration was inhibited by the transfection with BAFF-siRNA. KR33426 also inhibited BAFF expression increased by TNF-α treatment in FLS as judged by western blotting, PCR, and transcriptional activity assay. Kinases including JNK, p38 and Erk were activated by TNF-α treatment. While JNK and p38 were inhibited by KR33426 treatment, no changes in Erk were observed. Transcription factors including p65, c-Fos, CREB and SP1 were enhanced by TNF-α treatment. Among them, c-Fos was inhibited by KR33426 treatment. Small interference(si)-RNA of c-fos decreased BAFF transcriptional activity. FLS migration induced by TNF-α was inhibited by the transfection with BAFF-siRNA. KR33426 increased Twist, Snail, Cadherin-11 and N-Cadherin. In contrast, KR33426 decreased E-cadherin and TNF-α-enhanced CCL2. Taken together, our results demonstrate that synovial cell migration via CCL2 expression could be regulated by BAFF expression which is decreased by KR33426 and c-Fos-siRNA. It suggests for the first time that the role of BAFF-siRNA on FLS migration might be matched in the effect of KR33426 on BAFF expression.