• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1547-1556
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• 2009

A novel thermostable $\alpha$-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4 was successfully expressed in E. coli and characterized. The TtgluA gene contained 2,253 bp, which encodes 750 amino acids. The native TtGluA was a trimer with monomer molecular mass of 89 kDa shown by SDS-PAGE. The purified recombinant enzyme showed hydrolytic activity on maltooligosaccharides, p-nitrophenyl-$\alpha$-D-glucopyranide, and dextrin with an exotype cleavage manner. TtGluA showed preference for short-chain maltooligosaccharides and the highest specific activity for maltose of 3.26 units/mg. Maximal activity was observed at $60^{\circ}C$ and pH 5.5. The half-life was 2 h at $60^{\circ}C$. The enzyme showed good tolerance to urea and SDS but was inhibited by Tris. When maltose with the concentration over 50 mM was used as substrate, TtGluA was also capable of catalyzing transglycosylation to produce $\alpha$-1,4-linked maltotriose and $\alpha$-1,6-linked isomaltooligosaccharides. More importantly, TtGluA showed exclusive regiospecificity with high yield to produce $\alpha$-1,6-linked isomaltooligosaccharides when the reaction time extended to more than 10 h.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6343-6348
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• 2012

Objective: The NAD(P)H:quinone oxidoreductase 1 (NQO1) rs1800566 polymorphism, leading to proline-toserine amino-acid and enzyme activity changes, has been implicated in bladder cancer risk, but individually published studies showed inconsistent results. We therefore here conducted a meta-analysis to summarize the possible association. Methods: A systematic literature search up to August 27, 2012 was carried out in PubMed, EMBASE and Wanfang databases, and the references of retrieved articles were screened. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were analyzed for homozygote contrast (TT vs. CC), additive model (T vs. C), dominant model (TT+CT vs. CC), and recessive model (TT vs. CC+CT) to assess the association using fixed- or random-effect models. Results: We identified 12 case-control studies including 3,041 cases and 3,128 controls for the present meta-analysis. Significant association between NQO1 rs1800566 genetic polymorphism and risk of bladder cancer was observed in the additive model (OR = 1.15, 95% CI = 1.01-1.30, p = 0.030). Moreover, in the subgroup analysis stratified by ethnicity, significant associations were observed in Asians (OR = 1.26, 95% CI = 1.08-1.47, p = 0.003 for T vs. C; OR = 1.68, 95% CI = 1.21-2.32, p = 0.002 for TT vs. CC; OR = 1.50, 95% CI = 1.13-1.98, p = 0.005 for TT vs. CT+CC) but not in Caucasians. Conclusions: The results suggest that NQO1 rs1800566 genetic polymorphism may contribute to bladder cancer development, especially in Asians.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.11
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• pp.1721-1728
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• 2018

Objective: The use of genetic markers can help to enhance reproduction in cattle, which is a very important trait for profitability in dairy production systems. This study evaluated the association between genotypes of leptin (LEP), toll-like receptor 4 (TLR4), and chemokine receptor of interleukin 8 C-X-C motif (CXCR1) genes and fertility traits in Czech Fleckvieh cattle. Methods: Phenotypic data from 786 Czech Fleckvieh cows raised on 5 farms in the Czech Republic were used, along with information from the 1st three parities. To determine genotype, the polymerase chain reaction-restriction fragment length polymorphism method was used. Results: Except for LEP g.-963C>T, all studied genotype frequencies of single nucleotide polymorphisms (SNPs) were distributed according to the Hardy-Weinberg equilibrium. Two LEP SNPs (g.-963C>T and c.357C>T) were associated with the age at the 1st calving, days open (DO), pregnancy rate after 1st service (PR), and calving interval (CLI). In LEP g.-963C>T the TT genotype heifers firstly calved 24 days earlier than CC genotype and the CT genotype cow showed a tendency for shorter DO and higher PR. In LEP c.357C>T we observed longer CLI and DO period in TT cows. In general, we can propose the TT genotype of g.-963C>T as favorable and the TT genotype of c.357C>T as unfavorable for a cow's fertility. Heterozygotes in TLR4 c.-226C>G were significantly associated with shorter CLI, and presented a nonsignificant tendency to be associated with higher PR. In CXCR1 c.777 C>G, we did not observe any relationship of this SNP with reproduction. Conclusion: Overall, the results showed that LEP could be an effective marker for improving reproduction in Czech Fleckvieh cattle. This study also provides novel insights into the relationship between TLR4 and CXCR1 SNPs and reproduction in dual-purpose cattle.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.5
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• pp.36-41
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• 2009

In this paper an Investigation into the actual condition of electrical equipment installation for the inspection method development based on IEC 60364. The analysis objects are the difference between electrical equipments installation in korea & electrical equipments installation based on the IEC 60364. The measurement elements are loop impedance, operating characteristic of circuit breaker and protective conductor continuance. As a result, the korea electrical equipments installation was almost same the TT system but some different of protective conductor continuance. Electrical equipments installed TN-C-S has noting problem about installation but manager dose not understand about IEC 60364.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.12
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• pp.1359-1369
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• 2007

This paper describes the technique of satellite direction finding and main beam steering of the adaptive array antenna system which is used for mobile TT&C(Tracking Telemetry&Command) system. To be able to control the satellite on mobile vehicle while moving, the relative directional information of the satellite to the mobile vehicle is necessary to make main beam to the direction of satellite. To do this MUSIC, which is one of the super-resolution algorithm of wave direction finding, is used and then the performance analysis and quantization problem of phase shifter are addressed. This paper is valuable in the respect of showing feasibility of designing the moble TT&C using adative array antenna system.

• BMB Reports
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• v.47 no.7
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• pp.399-404
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• 2014

B7-H4 is a member of B7 family of co-inhibitory molecules and B7-H4 protein is found to be overexpressed in many human cancers and which is usually associated with poor survival. In this study, we developed a therapeutic vaccine made from a fusion protein composed of a tetanus toxoid (TT) T-helper cell epitope and human B7-H4IgV domain (TT-rhB7-H4IgV). We investigated the anti-tumor effect of the TT-rhB7-H4IgV vaccine in BALB/c mice and SP2/0 myeloma growth was significantly suppressed in mice. The TT-rhB7-H4IgV vaccine induced high-titer specific antibodies in mice. Further, the antibodies induced by TT-rhB7-H4IgV vaccine were capable of depleting SP2/0 cells through complement-dependent cytotoxicity (CDC) in vitro. On the other hand, the poor cellular immune response was irrelevant to the therapeutic efficacy. These results indicate that the recombinant TT-rhB7-H4IgV vaccine might be a useful candidate of immunotherapy for the treatment of some tumors associated with abnormal expression of B7-H4.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.9
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• pp.914-923
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• 2009

Helix antennas have been widely applied to satellite TT&C, data communication and GPS receiver systems onboard military, remote sensing and communication purpose satellites. The helix antennas are known to be convenient to control impedance and radiation coverage characteristics with a maximum directivity in satellite z-axis. Waveguide horn is commonly used for radar system that needs ultra-wideband pulse for exploration ground radar and electromagnetic disability measurement etc. It has high efficiency and low reflection characteristics provided by the low-profile shape and suppressed radiation distortion. In this paper, a waveguide horn structure incorporated with helix antenna design is proposed for satellite applications that require ultra-wideband pulse radar and high rate RF data communication link to ground station over wide coverage area. The main design concern is to synthesize variable beam forming pattern based on modified horn-helix combination helicone structure such that multi-mission antenna is implemented applicable for TT&C, earth observation, high data rate transmission. Waveguide horn helps to reduce the overall antenna structure size by introduction fold type reflector connected to the tapered helix antenna. The next generation KOMPSAT satellite currently under development requires high-performance precision attitude control system. We present an initial design of a hybrid hern-helix antenna structure suitable for efficient RF communication module design of multi-purpose satellite systems.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7337-7341
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• 2014

Background: The physiological role of vitamin D extends beyond bone health and calcium-phosphate homeostasis to effects on cancer risk, mainly for colorectal cancer. Vitamin D may have an anticancer effect in colorectal cancer mediated by binding of the active form $1,25(OH)_2D$ to the vitamin D receptor (VDR). The Taq1 VDR gene polymorphism, a C-to-T base substitution (rs731236) in exon 9 may influence its expression and function. The aim of this study wass to determine the 25(OH)D vitamin D level and to investigate the association between circulating vitamin D level and Taq1VDR gene polymorphism among Jordanian colorectal cancer patients. Materials and Methods: This case control study enrolled ninety-three patients and one hundred and two healthy Jordanian volunteers from AL-Basheer Hospital/Amman (2012-2013). Ethical approval and signed consent forms were obtained from all participants before sample collection. 25(OH)D levels were determined by competitive immunoassay Elecsys (Roche Diagnostic, France). DNA was extracted (Promega, USA) and amplified by PCR followed by VDR Taq1 restriction enzyme digestion. The genotype distribution was evaluated by paired t-test and chi-square. Comparison between vitamin D levels among CRC and control were assessed by odds ratio with 95% confidence interval. Results: The vitamin D serum level was significantly lower among colorectal cancer patients (8.34 ng/ml) compared to the healthy control group (21.02ng/ml). Patients deficient in vitamin D (less than 10.0 ng/ml) had increased colorectal cancer risk 19.2 fold compared to control. Only 2.2% of CRC patients had optimal vitamin D compared to 23.5% among healthy control. TT, Tt and tt Taq1 genotype frequencies among CRC cases was 35.5%, 50.5% and 14% compared to 43.1%, 41.2% and 15.7% among healthy control; respectively. CRC patients had lower mean vitamin D level among TT ($8.91{\pm}4.31$) and Tt ($9.15{\pm}5.25$) genotypes compared to control ($21.3{\pm}8.31$) and ($19.3{\pm}7.68$); respectively. Conclusions: There is significant association between low 25(OH)D serum level and colorectal cancer risk. The VDRTaq1 polymorphism was associated with increased colorectal cancer risk among patient with VDRTaq1 TT and Tt genotypes. Understanding the functional mechanism of VDRTaq1 TT and Tt may provide a strategy for colorectal cancer prevention and treatment.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.2216-2217
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• 2008

This paper write about earth system for the prevention electric shock and a protection of equipment in england. The IEC60364 and BSI(British Standards Institution) define earthing method(IT, TT, TN-C, TN-S, TN-C-S)-BSI is british standards and in case of england almost use the TN system. TN system can classify TN-S & TN-C-S(PME). The findings on survey in england are used the TN-S system and TN-C-S system about 70% and residue 30% are used TT system and IT system from the exiting state of things.

• The KIPS Transactions:PartC
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• v.8C no.3
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• pp.367-372
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• 2001

지도제작, 해양생태감시, 기상관측, 및 우주환경감시 등의 분야에 이용되는 저궤도 위성을 관제(위성추적, 원격측정 및 명령)하기 위해 본 논문에서는 최적의 PM 변조지수를 결정하는 방법을 기술하고 수신 데이터의 성능을 평가한다. 이 저궤도 위성인 아리랑 위성은 한국에서는 첫 번째 저궤도 위성이며 1999년 12월 발사되어 현재 임무를 충실히 수행하고 있다. 아리랑 위성 관제시스템의 파라미터에 의해 위성링크를 설계하면, PB 원격측정 신호의 최적 변조지수는 Eb/No마진과 PFD마진의 교차점이 되며 1.92라디안으로 결정된다. 또한 위성의 통과시간에 따라 예측되는 BER은 RT모드에서 최고의 성능을 나타애고, RT+RNG모드에서 최악의 성능을 나타내기 때문에 데이터 전송순서를 위성의 통과시간에 따른 BER 성능 순서인 RT, PB, RT+RNG모드의 순서로 결정함으로써 수신성능을 높일 수 있다.