• Journal of the Korean earth science society
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• v.28 no.1
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• pp.54-63
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• 2007

The sedimentary sequence of the non-marine Cretaceous Jinju Formation from Sacheon, Korea, contains a number of rod-shaped stromatolites (RSS) characterized by concentric lamination with curd-shaped, stratiform, and small columnar stromatolites. Unlike the world trend, a massive distribution of rod-shaped stomatolite was discovered in the region. The mineral composition, diagenetic alteration, and weathering process of the kind were analyzed by EPMA. The gross morphology of RSS is almost identical to broken plant twigs or stems formed by microbial activity onto which it grew. RSSs are interpreted as stromatolitic algae over plant twigs, which formed through concentric carbonate precipitation by epiphytic algal photosynthesis. The distribution of localities and horizons of the stromatolite imply that RSS is allochthonous and autochthonous. Two types of cyanobacterial filaments and one type of peen algal filament were discovered. The size frequency distribution of calcified filamentous microfossils found in stromatolite was $2.2{\mu}m\;and\;7.8{\mu}m$ in mean diameter of the former, $32.3{\mu}m$ in mean diameter of the latter. The cyanobacterial fossils played a key role in the formation of stromatolite, while the green algal filament was auxiliary stromatolite-builder stromatolites. The filamentous microfossils including trichome were found within the stromatolitic laminae.

• Korean Journal of Ecology and Environment
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• v.46 no.2
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• pp.301-309
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• 2013

Between May and August 2012, a massive cyanobacterial bloom with Anabaena has been occurred throughout the North Han River. Sampling was conducted at one station on each lake, L. Uham, L. Cheongpyung, and L. Paldang, where occurred a dense bloom, in 13 July. According to the microscopic examination, the blooms was dominated by one specific filamentous cyanobacterium Anabaena and other phytoplankton. Morphologically, previous literature proven that this Anabaena species is A. crassa (Lemmermann) Komark.-Legn. & Cronberg. However, identification of species in a mixed population is complicated due to limited morphological differences. Therefore, with live sample including trichome, akinete and heterocyst, the sequences of 16S rRNA gene of Anabaena isolates were cloned and analyzed, and three 16S rRNA gene sequences of 1188~1520 bp in length were obtained. It was shown from the homologous analysis results that the obtained 16S rRNA sequences were highly homologous to the relevant sequences of A. crassa in GenBank. The 16S rRNA sequences of 63 species were retrieved from GenBank, and the phylogenetic tree was constructed by using these sequences.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.625-629
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• 2016

A new Gomchwi cultivar ‘Gommany’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. Petiole trichome existed, but light of leaf back did not. Density of leaf vein was 5 degree in a newly developed cultivar ‘Gommany’. Plant height, leaf length, leaf width and petiole length were 47.7, 16.3, 21.2, and 31.4 ㎝, respectively in the 3 year of growth characteristics. Plant size was also higher than that of ‘Gondalbi’. Bolting and flowering time were Aug. 5 and Sept. 10, respectively. In contrast, Bolting time of ‘Gondalbi’ showed 25 days quite earlier bolting time compared to ‘Gommany’, Which led to earlier flowering time. ‘Gommany’ showed higher number of leaves (138) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was 97% higher in ‘Gommany’ (1,575 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Gommany’ showed higher leaf thickness (0.69㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (24.3 ㎏/㎠) compared to ‘Gondalbi’ (23.0㎏/㎠). ‘Gommany’ showed higher resistance in the susceptibility of powdery mildew compared to ‘Gondalbi’. ‘Gommany’ cultivar was registered as a novel variety with number 90 in The Minister of the Korea Forest Service on December 2015.

• Korean Journal of Plant Taxonomy
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• v.43 no.3
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• pp.208-222
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• 2013

In this paper, we conducted the taxonomic study of the tribe Epilobieae Endl. and concluded that a total of nine taxa, including one Chamerion (Raf.) Raf. ex Holub and eight Epilobium L., exist in Korea. Although C. angustifolium subsp. angustifolium has been placed traditionally either in Epilobium or Chamaenerion, it can be clearly distinguished from the species of Epilobium by having alternate leaves, slightly zygomorphic flowers, non-clefted petals, and equal length of 8 stamens, supporting the recognition of genus Chamerion. All but one species of Epilobium, E. platystigmatosum, was investigated for the surface of seeds using scanning electron microscope (SEM). The seed sculpture of Korean Epilobium can be classified into three types, i.e., papillose, reticulate, and ridged. E. ciliatum subsp. ciliatum is the only species Epilobium, which has the ridged seed sculpture. E. amurense subsp. cephalostigma can be distinguished from conspecific E. amurense subsp. amurense based on leaf shape, trichome shape and distribution, size and habit. Both E. fastigiatoramosum and E. palustre have entire leaf margins, but they can be distinguished based on leaf shape, stigma, and seed sculpture; the former has elongated elliptic leaves, capitate stigma, ridged seed sculpture, whereas the latter one has elongated lanceolate leaves, club-shaped stigma, and reticulate seed sculpture. Finally, we report the first record of E. platystigmatosum in Korea, and further comparative study including conspecific populations from Japan and China can clarify the occurrence of this taxon in Korea.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.337-346
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• 1993

Experiments were performed to find out the effects of simulated acid rain(SAR) of pH 4.0 or pH 2.5 for 12 weeks on stomatal resistance, wettability and anatomical changes in Quercus acutissima and Ginkgo biloba seedlings. As a control, distilled water with a pH 6.5 was also sprayed. Stomatal resistance of Q. acutissima and of G. biloba remarkably increased after exposure to SAR. SAR increased the wettability of Q. acutissima leaves measured with water blue solution and of G. biloba leaves measured by leaf contact angle method. Anatomical changes in the leaves of Q. acutissima affected by SAR were the partial damage of epidermis and parenchymatous cells. Scanning electron microscopical observation showed that the number of trichomes in the leaves of Q. acutissima treated with SAR markedly decreased and the erosion of epicuticular wax was significant. No distinct damage was found in the G. biloba leaves at pH 4.0, while epidermis and vascular tissue were collapsed at pH 2.5. No significant alteration of surface structures in this tree species was observed.

• Journal of Food Hygiene and Safety
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• v.28 no.2
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• pp.188-193
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• 2013

Anatoxin-a, saxitoxin and neosaxitoxin are produced by Aphanizomenon flos-aquae that is a sort of the cyanobacteria phylum. Therefore, it is not permitted for food materials in Korea. Traditionally, the classification of cyanobacteria has been based on morphological characters such as trichome width, cell size, division planes, shape, and the presence of character such as gas vacuole. But, some diagnostic features, such as gas vacuole or akinetes, can show variation with different environmental or growth conditions and even be lost during cultivation. Therefore, we developed detection method for functional foods containing Aph. flos-aquae by PCR. To design the primer, 16S rRNA region of Aph. flos-aquae, Spirulina laxissima, and Spirulina spp. registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for comparative analysis, BioEdit ver. 7.0.9.0. was used. As a result, we was design AFA-F1/AFA-R1 (363 bp) primer for the differentiation Aph. flos-aquae from chlorella, spirulina, green tea, and spinach. Also, it could be distinguished chlorella and spirulina products those are made to contain 1% Aph. flos-aquae.

• Korean Journal of Microbiology
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• v.45 no.3
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• pp.268-274
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• 2009

A photosynthetic cyanobacterium Arthrospira platensis, well known for health food supplement, was studied as a target species for atmospheric $CO_2$ removal as well as biomass production. Although the biomass of A. platensis was massively produced in many countries, the recovery cost of its biomass is still high. The purpose of this study was to develop the A. platensis mutant strains which have enhanced growth rate and floatation activity to reduce the recovery cost. A. platensis KCTC AG20590 was treated with 0.24% ethyl methanesulfonate (EMS) for 20 min at room temperature. The mutant strain A. platensis M20CJK3 was finally selected by its morphological and physiological features. The morphology of the mutant A. platensis M20CJK3 was changed from loose-coiled form to tight-coiled form showing short pitch. The growth and $CO_2$ uptake rate of A. platensis M20CJK3 were improved about 15% and 17% compared with A. platensis KCTC AG20590, respectively. The floatation activity of A. platensis M20CJK3 was enhanced in 2-fold compared with that of A. platensis KCTC AG20590. Soluble proteins extracted from two strains were analyzed by two dimensional electrophoresis (2-DE) and MALDI-TOF MS/MS. Among 15 protein spots induced in 2-DE analysis, two spots were the proteins related to photosynthesis and electron transfer system of the other cyanobacteria. As a consequence, it seems that the tight-coiled mutant A. platensis M20CJK3 has an advantage of high growth rate and floatation activity which are beneficial for the mass cultivation and recovery.

• Applied Microscopy
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• v.40 no.2
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• pp.101-108
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• 2010

Vitex negundo is an aromatic plant which releases a unique scent due to the presence of essential oil stored presumably within glandular trichomes. The focus of this research was to study developmental patterns of glandular trichomes in Vitex negundo leaves using electron microscopy. There are two types of glandular trichomes which develop on the leaf epidermis of Vitex negundo, peltate glandular type (PT) and capitate glandular type (CT). Structural features differ significantly depending on size and density, formation of secretory cavity, plastid, etc during developmental stages. In young leaves, undifferentiated PTs are densely distributed in the upper epidermis, but are not externally exposed in the lower epidermis because they are covered by non-glandular simple trichomes. Upon leaf development, PTs and CTs show clear structural differentiation in the upper and lower epidermis. PTs are composed of up to eight head cells (ca. 35~40 ${\mu}m$) and one stalk cell (ca. 5 ${\mu}m$), while CTs are composed of four head cells (ca. 10~15 ${\mu}m$) and 1~2 stalk cells (ca. 10 ${\mu}m$). Although secretory cavities develop on the secretory head cells, their size, structure, and formation proceed very differently depending on trichome type. In early development of PT, a large cavity with numerous secretory vesicles form rapidly from the head cells. In CT, however, only a small secretory cavity is formed, slowly relative to PT, without secretory vesicles. The PTs are considered to play an important role in releasing the aromatic components of Vitex negundo.

• Journal of Korean Society of Forest Science
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• v.72 no.1
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• pp.9-15
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• 1986

The taxonomy of the broad-leaf evergreen trees (4 genera, 5 species) of Lauraceae was studied by the morphological characteristics of stipules. The results obtained were summarized as follows; 1) The stipules of Lauraceae were arranged alternately with 5-31 stipules of simple leaf. The venations or stipules were basal reticulate and pinnated vein. The shapes of stipule blade were oblong, oblanceolate, rhomboid and oval. The stipule apex was acuminate and obtuse, the stipule base was attenuate, and the stipule margin was entire margin. The trichomes of stipules were sericeous, tomentose and villous. The five species of Lauraceae could be identified by the morphological characteristics of stipules. 2) The morphologically changing forms of stipule vein were classified into three groups; A form - the form of pinnate vein with the distinguished main vein (lozoste lancifolia), B form - the three vein grows into the form of basal reticulate vein (Neolitsea sericea, Cinnamomum japonicum and C. camphora), C form - the parallel vein grows into the form of basal reticulate vein (Marhilus thunbergii). 3) The changing forms of stipule's width from stipule "a" to "j" were three forms; (1) a>d>g>j form: C. japonicum, C. camphora and I. lancifolia, (2) a>cj,(aj,(a

• Korean Journal of Plant Taxonomy
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• v.47 no.2
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• pp.137-153
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• 2017

Fruit and seed morphological characteristics were investigated in 36 taxa of 20 genera of Korean Ranunculaceae. As a result, fruits were classified as the achene, berry, or follicle type. In the taxa with achene, the presence of trichomes and stoma on the surface of the fruit and a feature in which the endocarp distinctively develops into a one-layer sclereid were considered as useful characteristics. Moreover, in the taxa with follicles, the composition of the seed coat (bitegmic vs. unitegmic), the cell shape of the seed coat, the presence of trichomes, and the type of seed coat were useful for taxonomic characteristics in Korean Ranunculaceae. Based on the fruit and seed characteristics, Adonis is likely to be classified into Helleboroideae rather than Ranunculoideae, as it has bitegmic ovules. In addition, Enemion is similar to Eranthis in unitegmic ovule and the seed coat structure which has non-sclereid exotesta; therefore, it is reasonable to be placed in Helleboroideae. According to the results of this study, more in-depth research is needed regarding the taxonomic positions of Callianthemum and Actaea, which has the only berry fruit type.