• Journal of Microbiology and Biotechnology
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• v.10 no.4
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• pp.496-501
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• 2000

Escherichia cole NZN111, which is known as a pfl ldhA double mutant strin, was metabolically engineered to produce succinic acid by overexpressing malic enzyme into the E. coli controlled by a trc promoter. Fermentation studies were carried out in a LB medium by first growing cells aerobically to an $OD_{600}$ of 5. At this point, 0.01 mM IPTG was added to induce the overexpression of malic enzyme and the agitation speed was gradually lowered. When the culture $OD_{600}$ reached 11, a complete anaerobic condition was achieved by flushing with a $CO_3-H_2$ gas mixture. When NZN111(pTrcML) was cultured at $37^{\circ}C$, the final succinic acid concentration of 2.8 g/l could be obtained after 30 h of anaerobic cultivation. The fermentation results were analyzed by the calculation of metabolic fluxes. Metaolic flux analysis showed that about 85% of phosphoenolpyruvate (PEP) was converted to pyruvate, and further converted to malic acid by malic enzyme.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.887-890
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• 2008

Shindorim Station is the most important transfer station which is crossed Seoul Metro Line-2 and national rail traffic Kyungbu, Honam and Kyungin Line etc.. There is the lagest transfer passengers and the greatest rail traffic. Therefore to solve the congeted station problem, Shindorim station extension project has been planned. This project has very difficult many problems. One of them is undercrossing the national rail traffic ground without open-cut excavation. There are many undercrossing construction methods in our country. But this project is required the best safety. So the best applicable methods are investigated, which are Front-Jacking, NTR and TRcM. In Design stage Front-Jacking with PRS method which is gound reinforcing is applied.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.3
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• pp.300-305
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• 2005

Context-aware intelligent services are essential in ubiquitous computing and intelligent robots environments, which make decisions on which services to start with the consideration of surrounding contexts. In the ubiquitous and intelligent environments, context-aware service engines should be light-weighted due to the resource restrictions on the devices. This paper presents a context-aware service engine which is designed for light-weighted devices. The context-aware service engine has been designed with special attention to improve the execution speed and to minimize the memory requirement.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.583-585
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• 2016

The recent advances in wireless networks area have led to new techniques, such as small cells or full-duplex (FD) transmission, have also been developed to further increase the network capacity. Particularly, full-duplex communication promises expected throughput gain by doubling the spectrum compared to half-duplex (HD) communication. Because this technique permits one set of frequencies to simultaneously transmit and receive signals. In this paper, we focus on the binary power control for the users and the base stations in full-duplex multiple cellulars wireless networks to obtain optimal sum-rate under the effect interference and noise. We investigate with a scenario in there one carrier is assigned to only one user in each cell and construct a model for this problem. In this work, we apply the binary power control by the its simplification in the implemented algorithm for both uplink and downlink simultaneously to maximize sum data rate of the system. At first, we realize the 2-cells case separately to check the optimal power allocation whether being binary. Then, we carry on with N-cells case in general through properties of binary power control.

• Journal of the Korean Society for Precision Engineering
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• v.31 no.3
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• pp.253-259
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• 2014

This study proposes a map-based control method to improve a vehicle's lateral stability, and the performance of the proposed method is compared with that of the conventional model-referenced control method. Model-referenced control uses the sliding mode method to determine the compensated yaw moment; in contrast, the proposed map-based control uses the compensated yaw moment map acquired by vehicle stability analysis. The vehicle stability region is calculated by a topological method based on the trajectory reversal method. The performances of model-referenced control and map-based control are compared under various road conditions and driving inputs. Model-referenced control uses a control input to satisfy the linear reference model, and it generates unnecessary tire lateral forces that may lead to worse performance than an uncontrolled vehicle with step steering input on a road with low friction coefficient. The simulation results show that map-based control provides better stability than model-referenced control.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2007.04a
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• pp.320-323
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• 2007

웹 서비스와 같은 분산된 환경에서, 특정 서비스를 수행하기 위해서는 원격의 컴퓨터나 사이트상에서 다중 에이전트들의 협업을 통해 이루어진다. 이때 서비스는 여러 에이전트들의 복잡한 행위들에 의해 구성된다. 또한 지능적인 서비스를 위해서는 에이전트들의 상태정보, 목적정보, 그리고 계획정보 등을 이용한다. 특히 계획정보는 에이전트들이 일련의 행위들로 구성된다. 하지만 계획수립을 위한, 기존 연구들 대부분은 정적으로 기술된 서비스 명세와 초기상태 정보를 이용하여 특정 목표를 만족시키는 단일 계획 생성 방법을 연구해왔다. 따라서 계획수립이 실행 도중에 기대하지 않은 네트워크 장애나 방해 등으로 서비스 수행을 실패하는 경우, 그 계획은 무효가 되고 다시 계획을 생성 해야만 한다. 그러나 다시 계획을 생성하기 위해서는 많은 시간을 소비하게 될 뿐만 아니라 태스크 중복이 불가피하므로 매우 비효율적이다. 이 논문에서는 강건한 계획수립과 그 계획을 실행하기 위한 효과적인 방법을 제안한다. 즉, 계획수립의 재생성을 피하기 위한 방법으로 단일 계획수립 대신에 실행 가능한 다중 계획들로 표현된 강건한 계획을 생성하는 것이다. 강건한 계획의 행위들이 실행되는 동안, 각 단계마다 실행 가능한 행위를 선택한 후, 그 행위를 실행한다. 그러나 선택된 행위가 실행결과를 낼 수 없을 경우, 대체 가능한 서브 계획 경로를 선택하여 실행한다. 강건한 계획을 표현하기 위해 페트리 넷 기반의 방법을 제안한다. 강건한 계획 생성 방법에서는 이용 가능한 모든 계획들을 입력으로 사용한다. 그 계획수립 방법은 HTN 계획수립기로 잘 알려진 JSHOP2 계획수립기내에 구현하였다. 계획 실행 방법으로는 주어진 강건한 계획에 대하여 행위들이 직접 실행하수 있도록 한다.며 용량에 의존하는 양상을 보였다. $H_2O_2$에 의해 유발(誘發)된 DNA의 손상은 catalase와 deferoxamine에 의해 억제되었지만 DPPD는 억제시키지 못했다. 배기음(排氣飮)은 $H_2O_2$에 의해 유발(誘發)된 ATP의 소실을 회복시켰다. 이러한 실험결과 $H_2O_2$에 의해 유발(誘發)된 세포(細胞)의 손상(損傷)은 지질(脂質)의 과산화(過酸化)와는 다른 독립적인 기전에 의해 일어남을 나타낸다. 결론 : 이러한 결과들로 볼 때 Caco-2 세포(細胞)에서 배기음(排氣飮)이 항산화작용(亢酸化作用)보다는 다른 기전을 통하여 Caco-2 세포안에서 산화제(酸化劑)에 의해 유발(誘發)된 세포(細胞)의 사망(死亡)와 DNA의 손상(損傷)을 방지할 수 있다는 것을 가리킨다. 따라서 본 연구(硏究)는 배기음(排氣飮)이 반응성산소기(反應性酸素基)에 의해 매개된 인체(人體) 위장관질환(胃腸管疾患)의 치료(治療)에 사용할 수 있을 가능성(可能性)이 있음을 제시하고 있다.에 이를 이용하여 유가배양시 기질을 공급하는 공정변수로 사용하였다 [8]. 생물학적인 폐수처리장치인 활성 슬러지법에서 미생물의 활성을 측정하는 방법은 아직 그다지 개발되어있지 않다. 본 연구에서는 슬러지의 주 구성원이 미생물인 점에 착안하여 침전시 슬러지층과 상등액의 온도차를 측정하여 대사열량의 발생량을 측정하고 슬러지의 활성을 측정할 수 있는 방법을 개발하였다.enin과 Rhaponticin의 작용(作用)에 의(依)한 것이며, 이는 한의학(韓醫學) 방제(方劑) 원리(原理)인 군신좌사(君臣佐使) 이론(理論)에서 군약(君藥)이 주증(主症)에 주(主)로 작용(作用)하는 약물(藥物)이라는 것을 밝혀주는

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 1996.10b
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• pp.69-74
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• 1996

본 연구에서는 DSM 프로그램 개발에 있어 가장 일반적인 수행수단으로 활용되고 있는 리베이트의 적정수준 결정을 위한 방법과 절차를 제시하였다. DSM 기술의 비용효과 분석에 있어서는 전력회사의 회피비용과 기술비용을 각각 편익과 비용지표로 산정하였으며, 이를 토대로 RIM 및 TRC 지표에 의해 상이한 리베이트 수준에서의 시뮬레이션을 반복적으로 시행하였다. 적용 대상 프로그램으로는 현재 국내에서 시행되고 있는 고효율 조명프로그램의 전자식 안정기를 택하였다.

• Proceedings of the KIEE Conference
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• 2003.07a
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• pp.618-620
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• 2003

This paper presents a software for cost-effectiveness evaluation of DSM programs (Dsm-Lite). The presented software can help the users to analyze the energy consumptions and peak reductions of DSM programs, evaluate the cost effectiveness for TRC and RIM, and identify the benefits and the costs for each Test. The Dsm-Lite software can also provide the effective information on avoided costs, electric rates, and rebates by year etc.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.12
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• pp.2836-2843
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• 2015

Due to metallicity of materials, a vessel has a limitation to adopt RF-based wireless communication technologies for the inner communication means. Visible Light Communication(VLC) can be a sound alternative to dissolve such a limitation. Using a visual light as a transmission medium, VLC is free from radio interferences and restriction of radio usages which are typically related to RF-based wireless communications. In addition, VLC can not only require the facility cost relatively low because of being possibly converged with existing LED illumination, but also be harmless to the human body. This paper proposes an indoor location-based emergency call service system solution for ships using the VLC technology that supports 256Kbps data rate and 5m transmission distance. This paper presents real implementation and testing results of the solution which verifies the propriety of the proposal.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.560-567
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• 1998

We have found that Thermoactinomyces vulgaris KFB-Cl00 produces a chitinase. The optimum temperature and pH of the enzyme activity were $55^{\circ}C$ and 6.5. The enzyme was stable after heat treatment at $80^{\circ}C$ for 30 min and stable in acidic and basic conditions (PH 6.0~11.0). The thermostable endo-chitinase from Thermoactinomyces vulgaris KFB-C100 was cloned into the plasmid pBR322 by using E. coli DH5$\alpha$ as a host strain. The positive clone carrying a recombinant plasmid (PKCHI23) with a 4.1-kb fragment containing the chitinase gene was found. The recombinant plasmid was analyzed to determine the essential region for chitinase activity and obtained a 2.3-kb fragment, which was sub cloned into pTrc99A using the PstI and SalI sites to construct pTrc99A/pKCHI23-3. The resulting plasmid exerted high chitinase activity upon transformation of E. coli XL1-Blue cells. Chitinase was overproduced 14 times more in the clone cells than in the wild-type cells and the enzyme was purified to homogeneity. The purified enzyme showed the similar properties as the native chitinase from T. vulgaris in terms of molecular weight and substrate specificity. The catalytic action of the cloned enzyme was an endo type, producing chitobiose as a major reaction product.