• Korean Chemical Engineering Research
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• v.59 no.4
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• pp.514-520
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• 2021

A drug delivery system (DDS) based on nanoparticles has been used as a mediator to improve the efficacy of a drug by controlling the amount of drug released and delivering it to a target place. Chitosan, which is non-toxic and biodegradable, has good biocompatibility and excellent adsorption, so it can be used as a drug delivery vehicle. Valine, the essential amino acids, helps muscle growth and tissue recovery, and along with other amino acids. It lowers blood sugar levels and increases growth hormone production. In this study, Valine was adsorbed on magnetic chitosan which is capable of drug absorption, and Fe₃O₄-Valine CNPs was prepared through cross-linking with TPP (Tripolyphosphate). And then absorption and release trends of valine were investigated with the Fe₃O₄-Valine CNPs. Fe₃O₄, which has relatively high stability, is used to make the drug carrier magnetic so that the drug can be delivered to a target place. At optimal conditions, the absorption and release tendency of Fe₃O₄-Valine CNP was confirmed by analyzing by UV-Vis through the Ninhydrin test which is the color reaction of amino acids and by measuring the size of the particles, it was confirmed that it is suitable as a drug carrier.

• Korean Chemical Engineering Research
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• v.59 no.1
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• pp.21-34
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• 2021

Chitosan nanoparticles (CNPs) and Lactococcus lactis (L. lac.) were used as adsorbents to evaluate the adsorption performance of endocrine hormones, which are phthalates, in the healthy food packages. CNPs were produced through the cross bond with tripolyphosphate (TPP), and L. lac.-CNPs were prepared through the introduction of L. lac. during the preparation. The various functional groups of all adsorbents were identified using Fourier transform infrared spectroscopy (FTIR). Adsorption isotherm and adsorption kinetic confirmed the adsorption behavior and mechanism of CNPs, L. lac. and L. lac.-CNPs. The adsorption behavior of DBP and DEP for all particles was more suitable for the Freundlich adsorption isotherm model than for the Langmuir adsorption isotherm model, which means that the surface of the particles is heterogeneous. The adsorption mechanism was more suitable for the Pseudo-2nd-order model than for the Pseudo-1st-order model. This means that due to the presence of various functional groups on the particle surface, the adsorption of DBP and DEP is dominated by chemical adsorption such as electrostatic attraction and hydrogen bonding rather than physical adsorption. Finally, it was confirmed that the preparation of CNPs and L. lac.-CNPs can be performed easily and quickly, and it could be used as a cheaper adsorbent that can effectively remove phthalates.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.68
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• pp.149-169
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• 2015

Recently, it's a trend that the third party outsourcing logistics will be increased a logistics flow from law material to final products. The government also works on promoting logistics company as securing the expertise for strengthen logistics industry and secure competitiveness. Nevertheless, logistics market have been divided by polarization between small logistics company and major company. Also a labor who work for logistics is being caused by worsened working conditions. That is one of results of the survey. Almost 50% of response are they have not been aware of FTA and have not known to need the FTA. Many respondents said that entering the overseas logistics or a new way like FTA will be difficult to take part in. Therefore I suggest several examples in this paper to encourage a vitalization of small logistics company through the multilateral FTA. Now our country needs to make an effort to maximize connections between multilateral FTA and policy effect by signing FTA with many countries and extending Economic territory. This effect can completely cope with the improvement of economic interests, establishing regional integration strategy and change of global supply chain. Therefore, a small business logistics have to make their competitiveness & a new dealing market in the future.

• BMB Reports
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• v.31 no.3
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• pp.287-295
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• 1998

Acetolactate synthase (ALS) is the first common enzyme in the biosynthesis of L-Ieucine, L-isoleucine, and L-valine. The sulfonylurea-resistant ALS gene from Nicotiana tabacum was cloned into the bacterial expression vector pGEX-2T. The resulting recombinant plasmid pGEX-ALS3 was used to transform Escherichia coli strain XL1-Blue, and the mutant tobacco ALS (mALS) was expressed in the bacteria as a protein fused with glutathione S-transferase (GST). The fusion product GST-mALS was purified in a single step on a glutathione-Sepharose column. ALS activities of 0.9-2.5 ${\mu}mol/min/mg$ protein were observed in the GST-mALS, and the Km values for pyruvate, FAD, and TPP were 10.8-24.1, $(1.9-8.9){\times}10^{-3}$, and 0.14-0.38 mM, respectively. The purified GST-mALS was resistant to both the sulfonylurea and the triazolopyrimidine herbicides, and lost its sensitivity to end products, L-valine and L-leucine. For comparision, the tobacco wild-type recombinant ALS fused with GST, GST-wALS, was also characterized with respect to its pyruvate and cofactor bindings. These results suggest that the purified mutant recombinant tobacco ALS was functionally active, that the mutations resulting in herbicide resistance has affected pyruvate and cofactor bindings," and that the two classes of herbicides interact at a common site on the plant ALS.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.1
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• pp.175-182
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• 2005

A laser beam expanding technique is employed to fabricate precise nano-patterns in a nano-replication printing (nRP) process. In the nRP process, some patterns can be fabricated in the range of several microns inside on a polymerizable resin by using a volume-pixel (voxel) matrix that is transformed from a two-tone bitmap figure file. The liquid monomers are polymerized by means of a two-photon-absorption (TPA) phenomenon that is induced by a femtosecond (fs)-pulse laser. The yokels are generated consecutively to merge into adjoining yokels in the process of fabricating a pattern. The resolution of a fabricated pattern can be obtained under the diffraction limit of a laser beam by the two-photon absorbed polymerization (TPP). In this work, a beam-expanding technique has been applied to enlarge a working area and to fabricate precise patterns. Through this work, a working area is expanded by the technique as much as 2.5 times compared with a case of without a beam expanding technique, and precision of outside patterns is improved.

• Journal of Electrical Engineering and Technology
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• v.12 no.4
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• pp.1406-1416
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• 2017

In complex and large-scale industries, properly designed fault detection and identification (FDI) systems considerably improve safety, reliability and availability of target processes. In thermal power plants (TPPs), generating units operate under very dangerous conditions; system failures can cause severe loss of life and property. In this paper, we propose a bagged auto-associative kernel regression (AAKR)-based FDI approach for steam boilers in TPPs. AAKR estimates new query vectors by online local modeling, and is suitable for TPPs operating under various load levels. By combining the bagging method, more stable and reliable estimations can be achieved, since the effects of random fluctuations decrease because of ensemble averaging. To validate performance, the proposed method and comparison methods (i.e., a clustering-based method and principal component analysis) are applied to failure data due to water wall tube leakage gathered from a 250 MW coal-fired TPP. Experimental results show that the proposed method fulfills reasonable false alarm rates and, at the same time, achieves better fault detection performance than the comparison methods. After performing fault detection, contribution analysis is carried out to identify fault variables; this helps operators to confirm the types of faults and efficiently take preventive actions.

• The Journal of Society for e-Business Studies
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• v.12 no.1
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• pp.73-87
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• 2007

To survive in a global competition, many companies are trying to standardize and visualize Business Process (BP) by implementing Business Process Management (BPM). Recently, enhancing business process efficiency has become one of critical success factors. In this paper, we introduce a two-phase perspective of BP efficiency: Process Engine Perspective (PEP) and Task Performer Perspective (TPP). The former is related to allocation function of BP engine; it is mainly concerned with efficient task allocation to users. The latter phase influences efficiency depending on how users execute tasks assigned to them. Instead of considering each phase separately, we develop a comprehensive method considering the two-phase together, which is more effective for the BP efficiency. We carry out simulation experiment to show the combinational effect of the two phases.

• International Commerce and Information Review
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• v.14 no.1
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• pp.273-299
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• 2012

Japan's customs ensure the safety of international trade and trade facilitation has been pursuing. To do this, AEO was introduced to this system and expanding the supply chain currently maintain security and improve customer convenience is necessary to continue to be pursued. In addition, the imposition of customs duties in accordance with changes in the environment to Tariff offenders and increased the amount of tax evasion, smuggling of narcotics, including amphetamines and response, the increase in access is required. In this paper, Japan's tariff and customs administration for the change in the future for Japan-related materials around the Treasury Department's analysis will attempt to draw implications for this.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.108-108
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• 2011

Organometallic complexes containing unpaired spins, such as metalloporphyrin or metallophthalocyanine, have extensively studied with increasing interests of their promising model systems in spintronic applications. Additionally, the use of these complexes as an acceptor molecule in chemical sensors has recently received great attentions. In this presentation, we have investigated adsorption of nitric oxide (NO) molecules at Co-porphyrin molecules on Au(111) surfaces with scanning tunneling microscopy and spectroscopy at low temperature. At the location of Co atom in Co-porphyrin molecules, we could observe a Kondo resonance state near Fermi energy in density of states (DOS) before exposing NO molecules and the Kondo resonance state was disappeared after NO exposing because the electronic spin structure of Co-porphyrin were modified by forming a cobalt-NO bonding. Furthermore, we could locally control the chemical reaction of NO dissociations from NO-CoTPP by electron injections via STM probe. After dissociation of NO molecules, the Kondo resonance state was recovered in density of state. With a help of density functional theory (DFT) calculations, we could understand that the modified electronic structures for NO-Co-porphyrin could be occurred by metal-ligand hybridization and the dissociation mechanisms of NO can be explained in terms of the resonant tunneling process via molecular orbitals.

• Environmental Analysis Health and Toxicology
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• v.13 no.1_2
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• pp.1-9
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• 1998

The ROS-producing potency of chromium compounds of several oxidation states were determined in the H4 cells. $K_2Cr_2O_7$ as Cr (VI), synthetic Cr (V) compounds and Cr (III) as TPP produced high level of ROS. However, ROS values of Cr-picolinate as Cr (III), CrCl$_2$, CrCI$_2$, were almost equal to the control. The effects of physiological antioxidants compounds which react with free radicals were examined for their effects on chromate-induced production of reactive oxygen species (ROS) in A549 cells after the addition of $K_2Cr_2O_7$. The compounds used were vitamin C (ascorbate), vitamin E ($\alpha$-tocopherol), superoxide dismutase (SOD) and catalase. The preincubation of ascorbate (200uM) with A549 cells for 20hr resulted in a significant reduction of hexavalent chromate(100uM) induced ROS. However, there is no effects of preincubation of the cells with vitamin E succinate (10 and 20uM, 20hr) on the ROS production. Also, the effects of Cr (VI) on the cell cycle of A549 cells was measured by adding the DNA intercalating agent, propidium iodide. S phase of the cell cycle was increased by the chromium (VI) compounds up to 20uM indicating toxicity or possible mitogenic action of the cell. The shoulder in Go/G1 phase at 20uM Cr (VI) with 24 hr treatment indicates apoptosis.