• Journal of Korean Society on Water Environment
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• v.30 no.6
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• pp.632-637
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• 2014

Proficiency testing by interlaboratory comparisons is used to determine the performance of individual laboratories. In order to verify the quality of acute toxicity testing with Daphnia magna, National Institute of Environmental Research in South Korea is regularly organizing interlaboratory comparisons to estimate the analytical accuracy of different laboratories. Total 58 laboratories located in South Korea took part in interlaboratory proficiency testing scheme with three proficiency testing samples. TU(Toxic Unit) values of each laboratory were determined and robust z-score was calculated in order to evaluate the proficiency levels. Based on the robust z-score classification, 74% of the participant laboratories showed a satisfactory performance (43 laboratories). The main reason of 'unsatisfactory' performance seemed to be considered that the unsuitable management of test organism incubation system and the lack of experience on the identification of the test organism condition by effect of toxicity.

• Journal of Pharmacopuncture
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• v.19 no.3
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• pp.213-224
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• 2016

Objectives: Ginseng Rh2+ is enzyme-treated ginseng extract containing high amounts of converted ginsenosides, such as compound k, Rh2, Rg3, which have potent anticancer activity. We conducted general and genetic toxicity tests to evaluate the safety of ginseng Rh2+. Methods: An acute oral toxicity test was performed at a high-level dose of 4,000 mg/kg/day in Sprague-Dawley (SD) rats. A 14-day range-finding study was also conducted to set dose levels for the 90-day study. A subchronic 90-day toxicity study was performed at dose levels of 1,000 and 2,000 mg/kg/day to investigate the no-observed-adverse-effect level (NOAEL) of ginseng Rh2+ and target organs. To identify the mutagenic potential of ginseng Rh2+, we conducted a bacterial reverse mutation test (Ames test) using amino-acid-requiring strains of Salmonella typhimurium and Escherichia coli (E. coli), a chromosome aberration test with Chinese hamster lung (CHL) cells, and an in vivo micronucleus test using ICR mice bone marrow as recommended by the Korean Ministry of Food and Drug Safety. Results: According to the results of the acute oral toxicity study, the approximate lethal dose (ALD) of ginseng Rh2+ was estimated to be higher than 4,000 mg/kg. For the 90-day study, no toxicological effect of ginseng Rh2+ was observed in body-weight changes, food consumption, clinical signs, organ weights, histopathology, ophthalmology, and clinical pathology. The NOAEL of ginseng Rh2+ was established to be 2,000 mg/kg/day, and no target organ was found in this test. In addition, no evidence of mutagenicity was found either on the in vitro genotoxicity tests, including the Ames test and the chromosome aberration test, or on the in vivo in mice bone marrow micronucleus test. Conclusion: On the basis of our findings, ginseng Rh2+ is a non-toxic material with no genotoxicity. We expect that ginseng Rh2+ may be used as a novel adjuvant anticancer agent that is safe for long-term administration.

• Environmental Analysis Health and Toxicology
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• v.25 no.3
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• pp.207-214
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• 2010

Toxicity identification and quantification are important factors to evaluate the effect of industrial effluent on the aquatic environment. In order to measure the potential and real toxicity of mixed chemicals in the effluents, the biological method (i.e., WET test) should be used as well as chemical analysis method. In this study, we conducted WET test for various kinds of industrial effluents using aquatic organisms such as water flea (Daphnia magna), algae (Pseudokirchneriella subcapitata), fish (Oryzias latipes, Danio rerio), and microorganism (Vibrio fisheri). In addition, we carried out chemical analysis and TIE (Toxicity Identification Evaluation) for effluents in order to identify the substances causing toxicity. Among the 30 kinds of wastewater, S13 showed the highest eco-toxicity and $Ca^{2+}$ and $Cl^-$ ion were suspected as major compounds causing toxicity for aquatic organisms. In order to confirm these suspected compounds, various confirmation procedures need to be carried out.

• Journal of Korean Society on Water Environment
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• v.28 no.6
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• pp.943-953
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• 2012

Because of their unique properties, nano-sized metallic nanomaterials (NMs) have been used in extensive applications of biomedicine, electronics, optics, engineering, and personal care products. Accordingly, with the increasing release of NMs into the environment, numerous studies of nanoecotoxicity have been conducted. Fish embryo toxicity test (FET) has many benefits in evaluating toxicity of NMs as an alternative to a whole-body test in fish. In this study, we collected and analyzed the toxicity studies of metallic NMs on freshwater fish embryos. Most studies have demonstrated that metallic NMs are highly toxic during the early development of fish embryos. However, it should be noted that the results for the same NMs on the same test species show variation due to differences in the size or surface properties of the test NMs and exposure conditions. For the safe use of metallic NMs, we need to analyze their effects based on their properties, test species, environmental media, and diverse conditions.

• Journal of Pharmacopuncture
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• v.26 no.1
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• pp.86-93
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• 2023

Objectives: This study aimed to evaluate the potential toxicity of a recently developed and clinically used No-Pain pharmacopuncture (NPP) solution. We also assessed the lethal dose of the NPP agent following a single intramuscular injection in Sprague-Dawley (SD) rats. Methods: Animals were divided into two groups: the NPP test material group and the normal saline control group. A single intramuscular injection of the NPP agent (1.0 mL/animal) was administered to rats of the NPP test material group. The control group rats received the same volume of normal saline. Both female and male rats were included in each group. All rats were monitored for clinical signs and body weight changes for 14 days after administration of the test substance or saline. At the end of the observation period, a gross necropsy was conducted and localized tolerance at the injection site was analyzed. Results: No mortality was observed in the NPP test material and control groups. Moreover, no test substance-related effects were observed on clinical signs, body weight, necropsy findings, and localized tolerance at the injection site. Conclusion: The approximate lethal dose of the NPP agent is greater than 1.0 mL/animal under the conditions used in this study. Additional toxicity evaluations and clinical studies are needed to confirm the safety of NPP use in clinical practice.

• Environmental Analysis Health and Toxicology
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• v.25 no.2
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• pp.153-159
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• 2010

Perfluorooctane sulfonic acid (PFOS) and Perfluorooctanoic acid (PFOA) are the principal chemicals known as perfluoroalkyl acids (PFAs). Despite the widespread use of these compounds, relatively little is known about their fate and effects. The purpose of this study was to determine the toxic effects of PFOS and PFOA on Daphnia magna. In the acute toxicity test, D. magna were exposed for 48 hours at concentrations of 0, 30, 45, 67.5, 101.25 and 151.88 mg/L PFOS, and 0, 100, 160, 225, 337.5 and 506.25 mg/L PFOA, respectively. In the case of chronic toxicity test, D. magna were exposed through water for 21 days at concentrations of 0, 0.375, 0.75, 1.5, 3 and 6 mg/L PFOS, and 0, 1.25, 2.5, 5, 10 and 20 mg/L PFOA, respectively. Acute toxicity was assessed on the basis of immobility, while chronic toxicity was assessed on the basis of fecundity. The acute toxicity test on PFOS and PFOA showed that the values of $EC_{50}$ were 50.90 mg/L and 253.47 mg/L, respectively. In the chronic test, fecundity was reduced significantly at 1.5 mg/L of PFOS and 10 mg/L of PFOA, respectively. These results indicated that PFOS is more toxic to zooplankton than PFOA, and both chemicals have some hazard demonstrates risk for acute or chronic toxicity to freshwater organism.

• Environmental Analysis Health and Toxicology
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• v.21 no.2 s.53
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• pp.173-184
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• 2006

From the harmfulness expectation test conducted through a toxicity anticipation program, methylcyclohexane turned out to be harmful and simulative, but no carcinogenicity was anticipated. In a four-hour acute inhalation toxicity test, the result showed that lethal concentration ($LC_{50}$) was 3,750 ppm (15,054 mg/L), which was identified as a harmful substance on the basis of the harmful substance classification standard $2 of the Industrial safety and health law. methylcyclohexane fell under the category $4(2,500 substance from the GHS standard acute toxicity harmfulness classification. Also, from subchronic inhalation toxicity test that included 6 hours a day, five days a week, and for 13 weeks, we could observe weight, activity, long term weight, blood and blood biochemical influence from the exposure of test substance. No-observed effect level (NOEL) was determined below $100{\sim}400ppm$ inboth male and female. This material falls under the Category 2 ($50{\sim}250ppm/6hours/90days$) in the GHS (Globally Harmonized System) standard trace long-term whole body toxicity repeated exposure, and can be classified as a harmful substance in accordance with the Industrial Safety and Health Law harmful substance standard $NOEL{\leq}0.5mg/L/6hr/90day$ (rat).

• Journal of Sasang Constitutional Medicine
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• v.23 no.1
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• pp.79-86
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• 2011

1. Objectives: Pharmacopuncture according to Sasang Constitutional Medicine is new field of constitutional medicine. We investigated safety of pharmacopunctures to develop this treatment. 2. Methods: We searched for papers in fifteen Korean oriental medical journals and four online searching portal articles. Hepatotoxicity, nephrotoxicity and general toxicity tests were investigated about one hundred thirty kinds of pharmacopunctures. 3. Results: Toxicity test about 36 kinds of 130 pharmacopunctures were investigated. 1) Hepatotoxicity and nephrotoxicity test of pharmacopunctures were searched in 11 of 38 Soeumin herbs, 8 of 36 Soyangin herbs, 5 of 44 Taeeumin herbs and 1 of 12 Taeyangin herbs. From among these herbs, toxicity was detected in Radix aconiti lateralis preparata(附子), Fructus alpiniae oxyphyllae(益智仁), Semen crotonis(巴豆), Radix bupleuri(柴胡) and Semen armeniacae amarum(杏仁) pharmacopunctures. 2) General toxicity test of pharmacopunctures were searched in 8 of 38 Soeumin herbs, 9 of 36 Soyangin herbs, 6 of 44 Taeeumin herbs and 2 of 12 Taeyangin herbs. from among thes herbs, toxicity was detected in Radix aconiti lateralis preparata(附子), Radix ginseng(人蔘), Semen crotonis(巴豆), Rhizoma seu radix notopterygii(羌活), Radix aralia cordatae(獨活), Semen armeniacae amarum(杏仁) and Radix scutellariae(黃芩) and Cortex acanthopanacis(五加皮) pharmacopunctures. 4. Conclusions: From these results, we must decide extraction method, concentration and dose carefully to use the toxic pharmacopuncture. More toxicity test and further study is needed about pharmacopuncture treatment in Sasang Constitutional Medicine.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.229-235
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• 2008

Lack of honey bee toxicity data for most pesticide products used for strawberry restricts to predict the adverse effects to foraging honey bee after treatment of pesticide in plastic house. This study was conducted to evaluate the actual risk of worker honey bees (Apis mellifera L.) through acute contact toxicity test, acute oral toxicity test and toxicity of residues on foliage test with 21 pesticide products. The mortality of honeybee sprayed with 6 pesticides including dichlofluanid WP showed significantly different from control at recommended application rate in acute contact toxicity test at 24 hours after treatment. Fenpropathrin EC and milbemectin EC treatment groups showed more than 25% mortalities at recommended application rate in acute oral toxicity test. In toxicity of residues on foliage test, only fenpropathrin EC treatment group showed more than 25% mortalities at 10 days after treatment at recommended application rate. It was concluded that the most toxic route to exposure for honey bee is direct contact exposure to sprayed pesticides. Safety interval for honey bee was established by concerning the results of these tests.

• Journal of the Korean Society of Safety
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• v.30 no.4
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• pp.51-55
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• 2015

There are various studies on the fire suppression process but the study on second pollution from fire products is not enough yet. Therefor, in this study verify that environmentally-friendly properties($LC_{50}$) of foam extinguishing agent with increases its amount used through with Fish-Acute Toxicity Test using a fish named Misgurnus anguillicaudatus that is appointed by OECD Test Guideline. In conclusion, proven that environmentally friendly properties of the agent of hoseo university through 16 times of LC50 than that of market.