• 대한약침학회지
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• 제17권1호
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• pp.74-79
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• 2014

Objectives: This study was conducted to clarify the analgesic effect of toad cake and toad-cake-containing herbal drugs. Methods: We counted the writhing response of mice after the intraperitoneal administration of acetic acid as a nociceptive pain model and the withdrawal response after the plantar surface stimulation of the hind paw induced by partial sciatic nerve ligation of the mice as a neuropathic pain model to investigate the analgesic effect of toad cake and toad-cake-containing herbal drugs. A co-treatment study with serotonin biosynthesis inhibitory drug 4-chloro-DL-phenylalanine methyl ester hydrochloride (PCPA), the catecholamine biosynthesis inhibitory drug ${\alpha}$-methyl-DL-tyrosine methyl ester hydrochloride (AMPT) or the opioid receptor antagonist naloxone hydrochloride was also conducted. Results: Analgesic effects in a mouse model of nociceptive pain and neuropathic pain were shown by oral administration of toad cake and toad-cake-containing herbal drugs. The effects of toad cake and toad-cake-containing herbal drugs disappeared upon co-treatment with PCPA, but not with AMPT or naloxone in the nociceptive pain model; the analgesic effect of toad-cake-containing herbal drugs also disappeared upon co-treatment with PCPA in the neuropathic pain model. Conclusion: Toad cake and toad-cake-containing herbal drugs have potential for the treatments of nociceptive pain and of neuropathic pain, such as post-herpetic neuralgia, trigeminal neuralgia, diabetic neuralgia, and postoperative or posttraumatic pain, by activation of the central serotonin nervous system.

• Advances in Traditional Medicine
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• 제2권1호
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• pp.28-35
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• 2002

Toad venom, 'Chan su' in Chinese and 'somso' in Korean, is a well-known traditional oriental medicine obtained from the skin venom gland of the toad. Formulations of toad venom have been widely applied in China, Japan, Korea and other oriental countries for a long time. It is often found in traditional Chinese formulations, such as Jiuxin (or Kyushin in Japan), Yixin, Huoxin, Shexiang baoxin wan, Lu shen wan and Laryngitis pills. According to a pharmaceutical chemistry study, toad venom contains multiple biological active substances, such as bufalin, resibufogenin and cinobufagin. Modern pharmacological studies indicated that toad venom has multiple pharmacological actions, including acting as a cardiotonic, antitumor local anesthetic effects, stimulates the respiratory center, vasopressor action, anti-inflammatory and diuretic effects. Like other medications, toad venom also has certain toxicity and adverse effects, for example, inducing delayed afterdepolarization and triggered arrhythmia. The major chemical constituents, basic pharmacological actions and adverse reactions of toad venom are discussed in this article.

• 대한임상독성학회지
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• 제4권1호
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• pp.58-60
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• 2006

The venom gland of Toad contains large quantities of cardiac glycosides, and toad venom poisoning is similar to digitalis toxicity and carries a high mortality. Sometimes after ingestion of aphrodisiac pills which contain dried toad, a patient develops gastrointestinal symptoms and bradycardia, psychoneurologic symptoms. We have experienced 2 cases of toad venom intoxication, who ingested asian toads. Patients were presented to our ED with nausea, vomiting, and abdominal pain. The patients were peformed monitoring and conservative treatment and were fully recovered. Toad venom intoxication should be considered in patients with clinical manifestation of gastrointestinal irritation, cardiac arrhythmias, hyperkalemia, and detectable serum digoxin level without current medication of digoxin.

• 한국광학회지
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• 제16권1호
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• pp.21-26
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• 2005

40 Gbit/s 광 시분할 신호(OTDM:optical time-division-multiplexed)로부터 클럭 재생된 10 GHz 신호를 얻기 위해 고조모드잠금된 광섬유 레이저와 TOAD(Terahertz Optical Asymmetric Demultiplexer)를 이용하여 광 위상 동기 회로를 구성하였다. 입력된 40Gbit/s 광 신호 펄스로부터 TOAD를 통과한 펄스의 위상 정보를 이용하여 10 GHz로 위상 동기된 신호를 추출하였다. 추출된 10GHz RF 신호와 주변의 잡음 신호의 비는 40 dB 이상으로 측정되었다. 또한 TOAD에서 위상 정보 추출 과정에 대한 시뮬레이션을 수행하였다. 위상 동기 주파수의 작동범위는 입력 광 펄스의 기본 주파수를 중심으로 10 kHz 이내에서 측정되었다.

• 건축사
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• 통권524호
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• pp.15-15
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• 2012

• 환경생물
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• 제31권4호
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• pp.340-346
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• 2013

대구의 동남부지역에 위치한 망월지에서 2007년부터 2009년 사이에 일어난 두꺼비 유생의 집단폐사에 관한 연구이다. 망월지두꺼비를 대상으로 미생물학적 조직학적 검사를 시행하여 Aeromaona hydrophila를 분리해낼 수 있었다. A. hydrophila는 수계환경에 광범위하게 분포하는 기회감염균이다. 2007년~2009년 상승된 기온변화가 망월지내의 수온을 상승시킴으로서 어패류 및 양서류의 병원성 세균인 A. hydrophila의 급속한 증식이 유발되어 두꺼비유생을 감염시켜 집단폐사로 이어진 것으로 사료된다.

• 한국환경생태학회지
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• 제21권6호
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• pp.461-467
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• 2007

본 연구는 한국산 무당개구리의 먹이 습성을 알아보기 위하여 1990년 4월부터 1992년 9월까지 한반도 내륙지방 네 지역과 제주도 여덟 지역에서 채집된 487개체를 대상으로 이루어졌다. 내륙지방과 제주도 개체군사이의 개체 당 먹이 개체 수에는 차이가 없었으며, 보통 1-3개체의 먹이를 포식하지만 제주산에서는 94개체를 먹은 개체도 있었다. 내륙산 무당개구리의 먹이의 종류는 13목, 제주산은 10목이 조사되었고, 딱정벌레류와 벌류의 포식빈도와 포식된 먹이 개체수가 높게 나타났다. 두 개체군의 종류별 포식빈도는 비슷한 양상으로 나타났지만, 포식된 각 종류별 먹이 수는 다른 양상을 보였다. 내륙산 무당개구리가 보다 다양한 종류의 먹이를 포식하며 한정된 종류를 포식하지 않는 것으로 나타났다. 한국산 무당개구리의 월별 먹이 습성은 내륙산 무당개구리는 5월부터 10월까지, 제주산에서는 4월에서 9월까지 먹이 활동을 하는 차이점을 보였다. 포식된 먹이류 중에 딱정벌레류와 벌류는 조사기간 동안 한국산 무당개구리의 두 개체군에서 고르게 분포하였다. 한국산 무당개구리가 먹이로 삼는 목의 수, 포식빈도, 한 개체당 포식된 개체수가 모두 여름철에 높게 나와 가장 활발한 먹이 활동 시기는 여름철이라고 판단된다.

• Parasites, Hosts and Diseases
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• 제54권4호
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• pp.415-421
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• 2016

The drug-resistance of malaria parasites is the main problem in the disease control. The huge Brazilian biodiversity promotes the search for new compounds, where the animal kingdom is proving to be a promising source of bioactive compounds. The main objective of this study was to evaluate the antiplasmodial and cytotoxic activity of the compounds obtained from the toad venoms of Brazilian Amazon. Toad venoms were collected from the secretion of Rhinella marina and Rhaebo guttatus in Mato Grosso State, Brazil. The powder was extracted at room temperature, yielding 2 extracts (RG and RM) and a substance ('1') identified as a bufadienolide, named telocinobufagin. Growth inhibition, intraerythrocytic development, and parasite morphology were evaluated in culture by microscopic observations of Giemsa-stained thin blood films. Cytotoxicity was determined against HepG2 and BGM cells by MTT and neutral red assays. The 2 extracts and the pure substance ('1') tested were active against chloroquine-resistant Plasmodium falciparum strain, demonstrating lower $IC_{50}$ values. In cytotoxic tests, the 2 extracts and substance '1' showed pronounced lethal effects on chloroquine-resistant P. faciparum strain and low cytotoxic effect, highlighting toad parotoid gland secretions as a promising source of novel lead antiplasmodial compounds.

• 대한수의학회지
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• 제40권1호
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• pp.17-26
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• 2000

The regional distribution and relative frequencies of endocrine cells were studied immunohistochemically (PAP methods) in the alimentary tract and pancreas of the toad, Bufo bufo gargarizans Cantor using specific antisera against bovine Sp-1/chromogranin (BCG), serotonin, bombesin, gastrin, substance P (SP), somatostatin, insulin, glucagon, pancreatic polypeptide (PP), vasoactive intestinal polypeptide (VIP) and secretin. Nine kinds of endocrine cells were identified in this study. Spherical or spindleshaped immunoreactive (IR) cells were located in the gastric glands of stomach regions, in the basal portion of the epithelium of intestinal tract or esophagus, and in the exocrine or pancreatic islets with variable frequencies. In the alimentary tract, BCG-IR cells were found in the fundus and pylorus with rare and a few frequencies, respectively. Serotonin-IR cells were demonstrated in the whole alimentary tract including the esophagus. Bombesin- and SP-IR cells were restricted to the stomach regions and gastrin-IR cells were restricted to the pylorus. Somatostatin-IR cells were detected throughout the whole alimentary tract except for the large intestine, However, insulin-, glucagon-, PP-, VIP- and secretin-IR cells were not detected in the alimentary tract. In the pancreas of toad, the distribution and relative frequency of endocrine cells were similar to those of other mammals. Insulin-IR cells were located in the central portion of the pancreatic islets and interspaces of exocrine portions, and glucagon-, somatostatin- and PP-IR cells were detected in the marginal regions of the pancreatic islets and interspaces of exocrine. However, other IR cells were not found in the pancreas. In conclusion, the regional distribution and relative frequency of the endocrine cells in the alimentary tract and pancreas of the toad were similar to other anuran species but some differences which might be caused by feeding habits and species specification were also observed.

• Applied Microscopy
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• 제17권2호
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• pp.72-84
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• 1987

Histochemical and ultrastructural properties on the cutaneous mucous glands of the water toad, Bufo stejnegeri Schmidt, are studied with light and electron microscopes. Mucous glands of the water toad are simple alveolar glands which are composed of gland cavity in dermis and gland duct in epidermis. Each gland cavity of the mucous glands is consisted of outmost myoepithelial cells, glandular epithelial cells and inner cavity. Gland cavities of the mucous glands are composed of four kinds of glandular epithelial cells which are the dark cell, the light cell, the mucous containing cell and the small cell. Secretory materials which are certified as acid mucopolysaccharides by the histochemical reactions are synthesized through the Golgi complexes of the mucous containing cells.