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Research on the detection of LCN DNA from traces on firearms (총기 흔적흔에서의 low copy number(LCN) DNA 검출에 관한 연구)

  • Jeon, Chung-Hyun;Park, Sung-Woo
    • Analytical Science and Technology
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    • v.24 no.1
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    • pp.51-59
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    • 2011
  • Genetic Identification has become an important forensic investigation method which discerns identity through analysis of physical samples discovered in various crime scenes. Recently more samples are being requested to undergo A-STR analysis of low copy number (LCN) DNA, which is known as touch evidence-type sample and left on various objects such as a pen briefly used by the criminal, the gear of the car used for driving, the handle, and various buttons inside a car. This research attempted to extract the LCN DNA of the touch evidencetype left on crushed fingerprints on firearms, etc. and examine the genotyping success rate. Four types of firearms (M16, K1A, COLT 45 Pistol, M29 Revolver) were fired individually and physical samples were gathered from four parts of each firearm. Subsequently, in order to extract the LCN DNA, Microkit and $Prepfiler^{TM}$ were used to compare and analyze the quantity of DNA extracted and the genotyping success rate. Analysis results showed that the quantity of DNA extracted by $Prepfiler^{TM}$ was on average 1.7 times higher than that of Microkit, and in genotype analysis success rate $Prepfiler^{TM}$ also demonstrated 24.9% on average in contrast to 0% for Microkit. In regards to the grip part of the K1A, $Prepfiler^{TM}$'s success rate was as high as 50.6%.

A Study on Mathematics for Elementary Teachers (part 1) A Search for the Direction for Establishing the Concept of Mathematics for Elementary Teachers (초등학교 교직수학에 관한 연구(1) - 초등학교 교직수학의 개념 정립을 위한 방향 탐색)

  • 정은실;박교식
    • Journal of Educational Research in Mathematics
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    • v.9 no.2
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    • pp.405-418
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    • 1999
  • In this paper, we tried to establish the concept of 'Mathematics for Elementary Teachers(MET)'. There are 4 kinds of Mathematics for Teachers(MT). MET is one of tried to establish the concept in contradistinction to school mathematics(SM), mathematics, and Teaching Materials(TM). We suggested the outline of MET by suggesting the parts to which SM, MTs. The concept of MET is established variously according to various views. Here, we mathematics, and TM can not approach.

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Post-Marketing Surveillance Study of Hepatitis A Vaccine in Korean Population (한국에서 A형간염 백신(GlaxoSmithKline Biologicals' $Havrix^{TM}$)의 시판 후 조사)

  • Choi, Jae-Won;Kim, Myoung-Seok;Ma, Sang-Hyuk;Kang, Jin-Han;Ok, Jin-Ju;Ng, Timothy L.;Bock, Hans L.;HavrixTM PMS Korean study group,
    • Pediatric Infection and Vaccine
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    • v.15 no.2
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    • pp.115-120
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    • 2008
  • Purpose : This post-marketing surveillance (PMS) study was conducted in Korea to assess the safety and reactogenicity of hepatitis A vaccine (GlaxoSmithKlineBiologicals'$Havrix^{TM}$) administered to the Korean population. Methods : A total of 1,188 healthy subjects with 1,122 subjects <6 years of age were enrolled to receive one dose of the hepatitis A vaccine from April 2005 to January 2006. Diary cards were provided to the subjects or subjects' parents for reporting solicited and unsolicited symptoms during the 4-day and 31-day post-vaccination follow-up period. Results : The number of subjects who returned diary cards was 568, whereas, 620 subjects did not return diary cards. Among the subjects who returned diary cards, 9.9% and 14.3% reported local and general solicited/unsolicited symptoms. Among the subjects who did not return diary cards, 1.6% and 8.4% reported local and general solicited/unsolicited symptoms. At least one unsolicited symptom was reported by 13.2% of the subjects. Conclusion : Results indicate that the vaccine was well-tolerated and had an acceptable safety profile. The use of diary cards in such a survey provided a prompt and reliable option for recording symptoms.

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A Design of Clustering Classification Systems using Satellite Remote Sensing Images Based on Design Patterns (디자인 패턴을 적용한 위성영상처리를 위한 군집화 분류시스템의 설계)

  • Kim, Dong-Yeon;Kim, Jin-Il
    • The KIPS Transactions:PartB
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    • v.9B no.3
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    • pp.319-326
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    • 2002
  • In this paper, we have designed and implemented cluttering classification systems- unsupervised classifiers-for the processing of satellite remote sensing images. Implemented systems adopt various design patterns which include a factory pattern and a strategy pattern to support various satellite images'formats and to design compatible systems. The clustering systems consist of sequential clustering, K-Means clustering, ISODATA clustering and Fuzzy C-Means clustering classifiers. The systems are tested by using a Landsat TM satellite image for the classification input. As results, these clustering systems are well designed to extract sample data for the classification of satellite images of which there is no previous knowledge. The systems can be provided with real-time base clustering tools, compatibilities and components' reusabilities as well.

Conceptual Data Modeling: Entity-Relationship Models as Thinging Machines

  • Al-Fedaghi, Sabah
    • International Journal of Computer Science & Network Security
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    • v.21 no.9
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    • pp.247-260
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    • 2021
  • Data modeling is a process of developing a model to design and develop a data system that supports an organization's various business processes. A conceptual data model represents a technology-independent specification of structure of data to be stored within a database. The model aims to provide richer expressiveness and incorporate a set of semantics to (a) support the design, control, and integrity parts of the data stored in data management structures and (b) coordinate the viewing of connections and ideas on a database. The described structure of the data is often represented in an entity–relationship (ER) model, which was one of the first data-modeling techniques and is likely to continue to be a popular way of characterizing entity classes, attributes, and relationships. This paper attempts to examine the basic ER modeling notions in order to analyze the concepts to which they refer as well as ways to represent them. In such a mission, we apply a new modeling methodology (thinging machine; TM) to ER in terms of its fundamental building constructs, representation entities, relationships, and attributes. The goal of this venture is to further the understanding of data models and enrich their semantics. Three specific contributions to modeling in this context are incorporated: (a) using the TM model's five generic actions to inject processing in the ER structure; (b) relating the single ontological element of TM modeling (i.e., a thing/machine or thimac) to ER entities and relationships; and (c) proposing a high-level integrated, extended ER model that includes structural and time-oriented notions (e.g., events or behavior).

Cow's Milk-Related Symptom Score in Presumed Healthy Polish Infants Aged 0-6 Months

  • Bigorajska, Karolina;Filipiak, Zuzanna;Winiarska, Paulina;Adamiec, Anita;Trent, Bogumila;Vandenplas, Yvan;Ruszczynski, Marek;Szajewska, Hania
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.23 no.2
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    • pp.154-162
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    • 2020
  • Purpose: The Cow's Milk-related Symptom Score (CoMiSSTM), which considers crying, regurgitation, stools, skin and respiratory symptoms, was developed as an awareness tool for evaluating cow's milk-related symptoms. The scoring ranges from 0 to 33. A score ≥12 was proposed as being likely cow's milk-related and suggestive of allergy to cow's milk. This study aimed to determine the age-related CoMiSSTM values in presumed healthy infants in Poland. Methods: This was a cross-sectional study conducted in well-child clinics in two locations. Parents of the presumed healthy infants aged ≤6 months were approached during a routine checkup/vaccination visit. The exclusion criteria were as follows: presence of acute or chronic diseases, preterm delivery, treatment with therapeutic formula, and use of any food supplements (except vitamins) or medications. Results: Data from 226 infants were obtained (median age [Q1-Q3], 4 months [3-4]). The overall median (Q1-Q3) and mean (standard deviation) CoMiSSTM values were 4 (2-7) and 4.7 (3.5), respectively. The 95th percentile was 11. Scores on some, albeit not all, components of the CoMiSSTM significantly differed between age groups (crying, stools) or feeding type groups (stools and skin symptoms). Eleven children (4.9%) scored ≥12. Conclusion: This study adds to earlier age-related CoMiSSTM data by providing CoMiSSTM values in presumed healthy infants in Poland.

Comparison of computer-based treatment prediction with true results in bimaxillary protrusion cases (치조성 양악 전돌 환자에서 컴퓨터를 이용한 치료 결과 예측의 정확성)

  • Lee, Rhi-na;Lim, Yong-Kyu;Lee, Dong-Yul
    • The korean journal of orthodontics
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    • v.32 no.3 s.92
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    • pp.155-163
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    • 2002
  • This study was performed to assess the accuracy of computer-based treatment prediction for soft tissue profile using Quick Ceph Image $Pro^{TM}\;&\;Quick\;Ceph\;2000^{TM}$ in bimaxillary protrusion cases. The Ore- and post-treatment lateral cephalograms of 21 female adults treated by low first premolar extraction were imaged and 9 landmarks and 27 specific soft tissue mesurements were digitized for comparing actual treatment results with computer simulations. The results of this study showed that Quick Ceph Image $Pro^{TM}\;&\;Quick\;Ceph\;2000^{TM}$ tends to overestimeate horizontal changes and underestimate vertical changes. In the computer simulation, upper lip showed rolling tendency. The upper lip measurements were disposed to be regular direction hut lower lip measurements were varied case by case even if it was statistically insignificant.

Effect of aging on tear strength and cytotoxicity of soft denture lining materials; in vitro

  • Landayan, Jordi Izzard Andaya;Manaloto, Adrian Carlos Francisco;Lee, Jeong-Yol;Shin, Sang-Wan
    • The Journal of Advanced Prosthodontics
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    • v.6 no.2
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    • pp.115-120
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    • 2014
  • PURPOSE. The aim of this in vitro study was to evaluate the effect of aging on the tear strength and cytotoxicity of four soft denture lining materials. MATERIALS AND METHODS. Four commonly used soft denture lining materials, (Coe-Comfort$^{TM}$ GC America Inc., Alsip, IL, USA; Coe-SOFT$^{TM}$ GC America Inc., Alsip, IL, USA; Visco-gel Dentsply Caulk Milford, DE, USA; and Sofreliner Tough M Tokuyama Dental Corporation Tokyo, Japan) were selected. Sixty trouser-leg designed specimens per lining material were fabricated using a stainless steel mold for tear strength testing. The specimens were divided into non-thermocycling and 1000-, and 3000-thermocycling groups. For the cytotoxicity test, twenty-four disk shaped specimens per material were fabricated using a stainless steel mold. The specimens were soaked in normal saline solution for 24 h, 48 h and 72 h. Cytotoxicity was measured by XTT assay in L929 mouse fibroblasts. Data were analyzed by two way analysis of variance and Dunnett's test (P<.05). RESULTS. Before thermocycling, Sofreliner Tough M ($10.36{\pm}1.00N$) had the highest tear strength value while Coe-Comfort$^{TM}$ ($0.46{\pm}0.10N$) had the lowest. After 3000 cycles, Sofreliner Tough M ($9.65{\pm}1.66N$) presented the highest value and Coe-Comfort$^{TM}$ ($0.42{\pm}0.08N$) the lowest. Sofreliner Tough M, in all incubation periods was the least toxic with significant differences compared to all other materials (P<.05). Coe-Comfort$^{TM}$, Coe-$SOFT^{TM}$, and Sofreliner Tough M did not show any significant differences within their material group for all incubation periods. CONCLUSION. This in vitro study revealed that aging can affect both the tear strength and cytotoxicity of soft denture materials depending on the composition.

Impact of co-transfer of embryos produced by somatic cell nuclear transfer using two types of donor cells on pregnancy outcomes in dogs

  • Son, Young-Bum;Jeong, Yeon Ik;Jeong, Yeon Woo;Hossein, Mohammad Shamim;Hwang, Woo Suk
    • Animal Bioscience
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    • v.35 no.9
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    • pp.1360-1366
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    • 2022
  • Objective: The present study analyzed the influence of co-transferring embryos with high and low cloning efficiencies produced via somatic cell nuclear transfer (SCNT) on pregnancy outcomes in dogs. Methods: Cloned dogs were produced by SCNT using donor cells derived from a Tibetan Mastiff (TM) and Toy Poodle (TP). The in vivo developmental capacity of cloned embryos was evaluated. The pregnancy and parturition rates were determined following single transfer of 284 fused oocytes into 21 surrogates and co-transfer of 47 fused oocytes into four surrogates. Results: When cloned embryos produced using a single type of donor cell were transferred into surrogates, the pregnancy and live birth rates were significantly higher following transfer of embryos produced using TP donor cells than following transfer of embryos produced using TM donor cells. Next, pregnancy and live birth rates were compared following single and co-transfer of these cloned embryos. The pregnancy and live birth rates were similar upon co-transfer of embryos and single transfer of embryos produced using TP donor cells but were significantly lower upon single transfer of embryos produced using TM donor cells. Furthermore, the parturition rate for TM dogs and the percentage of these dogs that remained alive until weaning was significantly higher upon co-transfer than upon single transfer of embryos. However, there was no difference between the two embryo transfer methods for TP dogs. The mean birth weight of cloned TM dogs was significantly higher upon single transfer than upon co-transfer of embryos. However, the body weight of TM dogs did not significantly differ between the two embryo transfer methods after day 5. Conclusion: For cloned embryos with a lower developmental competence, the parturition rate and percentage of dogs that remain alive until weaning are increased when they are co-transferred with cloned embryos with a greater developmental competence.