• 생명과학회지
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• 제21권10호
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• pp.1481-1486
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• 2011

버섯 생산 후 발생되는 부산물인 팽이버섯 수확 후 배지로부터 7종의 고온성 균주를 분리하였으며 이 중 곰팡이독소를 생성하는 Asp. flavus와 Asp. ochraceous에 대한 항균활성이 높으면서 xylanase와 cellulase 생성능이 우수한 균주를 최종 선발하여 UJ03으로 명명하였다. Bacillus ID kit와 VITEK 2 system를 이용하여 분리균 UJ03의 생리적 생화학적 특성을 조사한 결과 분리균 UJ03은 Bacillus 속과 유사한 특징을 나타내었으며 16S rDNA 염기서열 분석 결과에서는 B. amyloliquefaciens와 98.9%의 상동성을 나타내었다. 이와 같은 결과를 종합하여 분리균 UJ03은 Bacillus sp. UJ03으로 동정되었으며 분리균 UJ03이 생성하는 항균물질은 TLC와 HPLC 분석에서 Bacillus 속 균주가 생성하는 펩타이드성 항균물질인 iturin A와 유사한 특성을 나타내었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7327-7330
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• 2015

Background: Essential thrombocythemia (ET) is a clonal hemopoietic stem cell myeloproliferative neoplasm characterized by persistent thrombocytosis along with megakaryocytic hyperplasia. In the last decade following the identification of an acquired JAK2 V617F mutation, there has been acceleration in our understanding of this disease. The rational of this study was to determine the mutational profile of JAK2 V617F in Pakistan patients with ET. Materials and Methods: In this retrospective cross sectional study, 21 patients with ET were enrolled from January 2011 to December 2014. Patients were diagnosed based on WHO criteria for essential thrombocythemia. Complete blood count was done on an automated hematology analyzer, while JAK2 V617F expression was evaluated by polymerase chain reaction. Results: The mean age was $56.7{\pm}19.0$ years (range 18-87) and the male to female ratio was 1:1.1. The frequency of JAK2 V617F positivity in our ET patients was found to be 61.9%. The mean hemoglobin was $11.7{\pm}2.4$ g/dl with a total leukocyte count of $13.3{\pm}8.1{\times}109/l$ and a platelet count of $1188{\pm}522{\times}109/l$. Positive correlations for JAK2 V617F mutation were established with high TLC count and raised LDH (P<0.05). No correlation of JAK2 V617F could be established with age and gender (P>0.05). Conclusions: JAK2 V617F mutation frequency in our ET patients was similar to those reported previously. Screening for the mutation in all suspected essential thrombocythemia cases could be beneficial in differentiating patients with reactive and clonal thrombocytosis.

• 셀메드
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• 제10권2호
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• pp.16.1-16.8
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• 2020

Standardization of drug deals with confirmation of drug identity and determination of drug quality and purity. Unani herbal formulations are used in traditional medicine for the treatment of various diseases. Cancer is a disease which causes abnormal, uncontrolled growth of body tissue or cells, which tend to proliferate in an uncontrolled way. Spread of cancer from site of origin to other organs of the body is called metastasis. It is a hyper proliferative disorder involving, transformation, dysregulation of apoptosis, invasion and angiogenesis. The present study aimed to standardize a classical Unani formulation (CUF) described as anticancer properties. The CUF has been used for anti-cancerous activity (Dāfi'-i-saraṭān) in human population by Unani physicians for centuries. The standardization parameters carried out for classical Unani formulation are pharmacognostical studies, physicochemical parameters, high-performance thin layer chromatography (HPTLC), microbial load, aflatoxins, and heavy metals revealing specific identities and to evaluate Pharmacopoeial standards. Experiment and the data obtained established the Pharmacopoeial standards for this formulation for identification and quality control purpose. The CUF has been successfully standardized and standard operating procedures (SOPs) for its preparation has been laid down which may serve as a standard reference in future. The standardization data of this formulation may be used as a standard guideline for preparation of the formulation in future.

• 한국도로학회논문집
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• 제17권4호
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• pp.53-61
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• 2015

PURPOSES : The objective of this study is to evaluate the SDAR (solvent deasphaltene residue), which is obtained from the solvent deasphalting (SDA) process, as a pavement material. METHODS : The physical properties of the SDAR were evaluated based on its chemical composition, and asphalt mixtures with the SDAR were fabricated and used for the evaluation of mechanical properties. Firstly, the chemical composition of SARA (saturate, aromatic, resin and asphaltene) was analyzed using the TLC-FID (thin-layer chromatography-flame ionization detector). Moreover, the basic material properties of the asphalt binder with the SDAR were evaluated by the penetration test, softening point test, ductility test, and PG (performance grade) grade test. The rheological properties of the asphalt binder with the SDAR were evaluated by the dynamic shear modulus ($G^*$) obtained using the time-temperature superposition (TTS) principle. Secondly, the mechanical properties of the asphalt mixtures with the SDAR were evaluated. The compactibility was evaluated using the gyratory compacter. Moreover, the tensile strength ratio (TSR) was used for evaluating the moisture susceptibility of the asphalt mixtures (i.e., susceptibility to pothole damage). The dynamic modulus $E^*$, which is a fundamental property of the asphalt mixture, obtained at different temperatures and loading cycles, was used to evaluate the mechanical properties of the asphalt mixtures. RESULTS AND CONCLUSION : The SDAR shows stiffer and more brittle behavior than the conventional asphalt binder. As the application of the SDAR directly in the field may cause early failures, such as cracks on pavements, it should be applied with modifiers that can favorably modify the brittleness property of the SDAR. Therefore, if appropriate additives are applied on the SDAR, it can be used as a pavement material because of its low cost and strong resistance to rutting.

• 한국미생물·생명공학회지
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• 제18권5호
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• pp.496-500
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• 1990

Pepsin(8mg/ml)에 의한 0.02 casein의 효소적 가수분해반응에 저해물질의 저해활성은 저해물질농도 20Mu/gml까지는 비례관계였으며,$IC_{50}$ 은 15${\mu}g$/ml였다. 저해물질의 pH 안정성은 pH5-9 범위내에서 $100^{\circ}C$, 10분 가열하였을 때 안정하였고, 열안정성은 pH7.0, $100^{\circ}C$에 20분까지는 100 저해활성이 나타나 비교적 안정하였다. 효소-저해물질 복합체는 형성되었으며 Lineveaver-Burk plot상에서 비경쟁적 저해양식이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.1001-1006
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• 2011

To determine the efficiency of copper (Cu) supplementation, a feeding experiment was carried out with 240 day old broiler chicks (vencobb-100). Birds were divided into four dietary treatments: i) C - no additives, ii) $T_1$-75 mg inclusion of Cu/kg diet, iii) $T_2$-150 mg inclusion of Cu/kg diet, iv) $T_3$-250 mg inclusion of Cu/kg diet. The present study was carried out in the Department of Animal Physiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India for a period of 42 days (6 weeks). Growth performance was measured in terms of live weight gain, cumulative feed intake and feed conversion ratio at the end of $21^{st}$ and $42^{nd}$ day of the experiment and the result was found to be encouraging for commercial enterprises when the chickens were fed at 150 mg Cu/kg ($T_2$) of their diet. Excess dietary copper more than 150 mg/kg reduced the haemoglobin (Hb) concentration in blood and resulted in the accumulation of copper in the liver with decreased blood Hb concentration and packed cell volumes (PCV). Copper supplementation increased the total erythrocyte count (TEC) as copper is involved in erythropoiesis. But, from the result it is indicated that the dietary copper concentration could not alter the total leukocyte count (TLC). In case of different leucocytes count (DLC), there were no significant differences observed among the different treated groups. Statistical analysis showed significant (p<0.01) difference in plasma concentration of copper, zinc, ferrous and cholesterol among the different copper treated groups. The result indicates that supplementation of copper is an effective way of improving the production performance and haematological parameters in broiler chicken.

• KSBB Journal
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• 제11권3호
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• pp.317-322
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• 1996

식품 및 화장품의 첨가제로 사용되는 기능성 올리고당인 갈락토올리고당을 생룰로스 가수분해 효소를 이용하여 생산하였다. 여섯가지의 효소 시료 가운데, P Penicillium funiculosum에서 유래한 셀룰로스 가수 분해 효소가 가장 높은 올리고당 생산 수율을 보였다. 올리고당 생산을 위한 최적온도와 pH는 각각 $50^{\circ}C$,와 5.0이었으며 유당농도와 효소농도사at의 최 적비는 lO(w/w) 이었다. 시간에 따른 올리고당 생산 반응의 경향성은 유당분해 효소 반응과 유사하였 으며 초기 유당농도가 증가함에 따라 생성되는 올리 고당의 농도가 증가하였다. 최척반응조건에서 20% (w/v)의 유당으로 부터 최고 23%(w/w)의 올리고 땅을 얻을 수 있었다. 생성된 올라고당의 성분은 고 속액체 크로마토그래프와 박층 크로마토그래프 분석 을 통하여 삼당과 사당으로 이루어져 있음을 확인하였다.

• Preventive Nutrition and Food Science
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• 제13권1호
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• pp.7-11
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• 2008

To improve the utilization of fish processing by-products, fish oils were extracted from hoki, yellowfin sole, mackerel, and horse mackerel, and their compositions were examined. The proximate compositions obtained for these 4 species of by-product revealed they were composed of 68.1$\sim$78.1% moisture, 1.2$\sim$1.6% ash, and 13.8$\sim$18.8% protein. Fish oils extracted from the hoki, yellowfin sole, mackerel, and horse mackerel were 5.5, 9.4, 13.4, and 10.3%, respectively. The total lipids extracted from the by-products of the 4 species were 6.21, 10.43, 12.81 and 10.06%, of which neutral lipids accounted for 77.38, 77.46, 87.21 and 86.79%, respectively. Neutral lipid analysis by TLC showed that triacylglycerol was the major component, while 1,3- and 1,2-diacylglycerols, free fatty acids, free sterols, and sterol esters were present as minor components. The major fatty acids were palmitic acid, stearic acid, and oleic acid. DHA and EPA were contained at levels of 0.2$\sim$4.7% and 3.7$\sim$9.5%, respectively, in the 4 types of fish oil. The fish oils extracted from the dark muscle fish, mackerel and horse mackerel, had greater polyunsaturated fatty acid (PUFA) contents than those of the white muscle fish species, hoki and yellowfin sole.

• 한국식품위생안전성학회지
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• 제5권3호
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• pp.131-138
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• 1990

Aflatoxins is a chemically diverse group of toxic secondary metabolites that are produced by fungi and often occur in agricultural commodities. Because of their wide range of toxic effects, Aflatoxins cause severe economic losses to farmers and livestock producers and pose a health to human consuming contaminated foods. Long term prospects for biotechnological control of Aflatoxins require elucidation of the specific steps and regulation of their biosynthetic pathways . Aflatoxin determinations can be approached many ways. It is essential to safely handle all experimental materials associated with aflatoxin analysis or aflatoxigenic fungi Visual screening of suspect samples, base on the presence of conidial head of the aspergillus flavus group, and screening samples for the presence of bright greenish yellow flourescence are not chemical tests and such screening techniques may allow aflactoxin contaminated lots into commerce. Microcolumn screening procedures should always be used in conjunction with a quantitative method. Several thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) methods are suitable for quantitation and are in general use. Immunochemical Methods such as the ELISA or affinity column chromatography methods are being rapidly developed. The chemical and immunochemical methods can be reliable if care is taken, using suitable controls and personnel that are well trained . All analytical laboratories should stress safety and include suitable analytical validation procedure. Especially a worldwide enquiry was undertaken in recent to obtain up-to-date information about aflatoxin legislation in as many countries of the world as possible. The information concerns aflatoxin in foodstuffs. aflatoxin MI in dairy products, aflatoxins in animal feedstuffs. Limits and regulations for aflatoxin have been expended in recent with more countries having legislation on subject, more products, and more aflatoxins covered by this legislation.

• 펄프종이기술
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• 제48권1호
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• pp.127-133
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• 2016

This study is performed that furniture and interior materials of MDF's (Medium Density Fiberboard) biodegradation properties, and the goal of this study is investigation of possibility of waste-MDF's composting after landfilling. To investigate biodegradation, this study was performed according to KS M ISO 14855-1, and there were two different soil conditions including a compost condition and an activated vermiculite condition as artificial soil. This experiment was tested for 40 days. The measurement of carbon-dioxide generation was processed every 24 hour in 1-2 week, and every 48 hour after 3 week. In the same days, MDF showed 24.4% of biodegradation in compost condition, and 6.2% in activated vermiculite. Also, the reference material of TLC (thin-layer chromatography) grade cellulose showed 26.4%, 11.4% in compost and activated vermiculite respectively. The dilution plate method was performed for biological analysis in the study. This experiment was used for investigation of inoculum's (Bacillus licheniformis) activity. As the result of bioassay, compost has more other germs include inoculum than activated vermiculite in the first week. Especially in the 2nd week, the reference material under the compost condition showed the most germ's activity, and also the biodegradation was the highest. Consequentially, compost condition was able to reduce a performing period of biodegradation testing than activated vermiculite. However, activated vermiculite could be stabilizing errors between repetition.