• Journal of the Korean Chemical Society
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• v.39 no.1
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• pp.40-46
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• 1995

A new analytical luminescence method for the Tb+3 and Eu+3 ions was studied using the fluorescence enhancement of the ions on the TLC plate. Compared to the specific emission intensities of the ions in aqueous or ethanol solution, if spotted on the TLC plate, the line intensities were extremely enhanced. There was additional enhancement effect of the lines from the ions on the TLC plate, if treated with ο-phenanthroline. Based on the luminescence enhancement, the detection limit of the ions was lowered more than 6 order of magnitude compared to the luminescence method using solution samples. The energy-transfer mechanism was also explained for the theoretical back ground of the luminescence enhancement.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.732-738
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• 2001

This study was attempted to isolate and identify the anticancer compounds from Eucommia ulmoides leaves using a human colon cancer cell line HCT-116. The petroleum ether extracts with anticancer activity was chromatographed on silica gel TLC and finally anticancer compounds was purified by HPLC. Their chemical structures were roughly elucidate by UV-VIS absorption spectral data HPLC elution pattern and FAM/MS spectroscopy. From this study these compounds were suspected to be pheophytin a formed by the removal of $Mg^{2+}$ from chlorophyll a and pyropheophytina formed by the removal of acetate group from pheophytin a respectively. To confirm the anticancer effects against HCT-116 cancer cell petroleum ether extract fractions of column chromatography and fractions separated on TLC were tested. All samples tested including the extract of petroleum ether fractions of column chromatograph and three bands (0.13,0.19,0.25) of TLC appeared to inhibit the growth of HCT-116 cancer cell however especially 0.19 and 0.25 fractions separated on TLC plate revealed the strongest effect. These results suggest that chlorophyll derivatives in Eucommia ulmoides may be potential anticancer agents against a human colon cancer cell HCT-116.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.1
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• pp.89-92
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• 2010

In the previous study, we reported the antioxidative activity, antiaging activity, antibacterial activity and moisturizing effect of cream containing Persicaria hydropiper L. extract. In this study, the components of Persicaria hydropiper L. extract were analyzed by TLC and HPLC. Aglycone fractions obtained from the deglycosylation reaction of ethyl acetate fraction among the Persicaria hydropiper L. extract, showed 2 bands and 2 peaks in TLC and HPLC experiments, respectively. Two components were identified as quercetin and kaempferol. TLC chromatogram of ethyl acetate fraction of Persicaria hydropiper L. extract revealed 6 bands and HPLC chromatogram showed 7 peaks, which were identified as quercetin, hyperin, isoquercitrin, quercitrin, kaempferol. In conclusion, with the antioxidative activity, antiaging activity, antibacterial activity and moisturizing effect reported previously, component analysis of Persicaria hydropiper L. extracts could be applicable to new cosmeceuticals.

• Journal of Periodontal and Implant Science
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• v.29 no.2
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• pp.311-326
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• 1999

This study was investigated to observe the effect of Treponema denticola(TDC) and Treponema lecithinolyticum(TLC) on cultured human periodontal ligament cells. Several experiments were performed including MTT test for the inhibition effect of cell proliferation, LDH test for the cytotoxicity , gelatin zymography for the gelatinase activation and observation of cell morphology change using the phase-contrast microscopy. The results were as follows. 1. The effect of concentration on cell proliferation with time showed an inhibitory effect at high concentration $(150{\mu}g/well)$ for TLC and at low concentration( $9.4{\mu}gwell$ ) for TDC. 2. The effect of time on cell proliferation with concentration showed an inhibitory effect at $150{\mu}g/well$ on 2-day incubation for TLC and at $9.4{\mu}g/well$ on 2-day incubation for TDC. 3. The effect of heat-treated TDC and TLC on the inhibition of cell proliferation showed the difference in the heat-treated group compared to the non-heat treated group for TDC, whereas no difference was found for TLC. 4. The morphological changes which were observed from the phase-contrast microscopy showed the difference in the test group compared to the control group. The loss of spindle-like appearance, cell-to-cell detachment and inhibition of cell proliferation were observed. 5. There was no difference of the cytotoxicity effect between the test group and the control group in the LDH test. 6. The active form of progelatinase A with molecular weight 72kDa was activated in both TDC and TLC on the gelatin zymography. Regarding to the above results, TDC and TLC have an effect on periodontal ligament cells by playing an inhibitory role in cell proliferation and appears to activate progelatinase A which degrades type IV collagen.

• Tuberculosis and Respiratory Diseases
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• v.54 no.3
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• pp.330-337
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• 2003

Background : Low spirometric forced vital capacity(FVC) in conjunction with a normal or high ratio of the forced expiratory volume at 1 second to the forced vital capacity($FEV_1$/FVC%) has traditionally been classified as a restrictive abnormality. However, the gold-standard diagnosis of a restrictive pulmonary impairment requires a measurement of the total lung capacity (TLC). This study was performed to determine the predictive value of spirometric measurements of the FVC for diagnosing a restrictive pulmonary abnormality. Methods : Test results from 1,371 adult patients who undertook both spirometry and lung volume measurements on the same visit from January 1999 to December 2000 were enrolled in this study. The test values for the FVC, the TLC that was below 80% of predicted value, and a $FEV_1$/FVC% that was below 70%, were classified as being abnormal. Results : Of the 1,371 patients, 353 patients had a reduced a FVC. Of these patients, 186 patients had a reduced TLC. Therefore, the positive predictive value was 52.7%. Of the 196 patients with a normal $FEV_1$/FVC% and a reduced FVC, 148(75.5%) patients had a lower TLC. Thirty eight (24.2%) patients out of 157 patients with a low $FEV_1$/FVC% and a low FVC showed a restrictive defect. Conclusion : Spirometry is useful to rule out a restrictive pulmonary abnormality, but a restrictive pattern on the spirometry dose not mean there is a true restrictive disease. For the patients with a low FVC, TLC measurements are essential for diagnosing a restrictive pulmonary impairment.

• KSBB Journal
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• v.19 no.4
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• pp.269-273
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• 2004

A simple, fast and reproducible quantitative analysis method for sugar concentration composed in oligosaccharide mixture was developed. Two glass TLC plates were prepared per sample. After dipping one plate into the copper bicinchoninate reagent and the other plate into 5% sulfuric acid solution, both plates were baked in microwave oven until sugar spots were developed or the surface temperature of TLC plate becomes 60 to 70 $^{\circ}C$. The corrective factor values [F value =(the value of total sugar concentration converted as glucose unit/the value of reducing sugar concentration converted as glucose unit)/(polymerization degree of sugar)] of different molecular weight sugars were determined. Within the concentration of 0.25∼1.0 $\mu\textrm{g}$ in each sample loaded, the fructose-F (corrective factor value of fructose) was 0.45, yet for the higher concentration (2.5∼7.5 $\mu\textrm{g}$) fructose-F was 1.0. In case of glucose, in the range of 0.5∼7.5 $\mu\textrm{g}$, glucose-F was same as fructose-F, 1.0. However, as the molecular weight of sugar was increased, the F values were decreased in both maltodextrin and isomaltodextrin oligosaccharides in 0.5∼7.5 $\mu\textrm{g}$ of each sample loaded. Interestingly, F values were equal for the same molecular weight sugars, although the structures were different from each other. Using F value of each sugar, we could determine and compare the exact total sugar concentration of different molecular weight maltooligosaccharide and isomaltooligosaccharide. We also could determine if the unknown sugar was a reducing or non-reducing compound by using optimized TLC with microwave oven method.

• Analytical Science and Technology
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• v.28 no.2
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• pp.79-85
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• 2015

A preliminary experiment was performed to develop a fast, convenient, and economical semi-quantitative method of analyzing amphetamine-like amines from images of derivatives. These were generated from the reaction (in situ, co-spot) of three amphetamine-like compounds with three derivatization reagents on a TLC plate. The attempt was made to optimize the reaction conditions for an efficient derivatization reaction, and TLC images taken by a digital camera were analyzed using two types of image analysis program (CP Atlas 2.0 and ImageJ) for repeatability (RSD, %) and linearity (R²). Then, their results were compared. For efficient derivatization, the reaction conditions needed to be modified. The results of image analysis of each of the samples at two different concentrations (0.5 mg/mL and 0.01 mg/mL) showed that the RSD values for reaction repeatability were in the range of 0.69-5.50%. From the calibration curves between the area of the derivative and the concentration of amines, the R² values (R² > 0.9906) for good linear correlation were found to be high, in a concentration range of 0.1-0.005 mg/mL of amines. In addition, the two programs demonstrated little difference in the analysis of repeatability and linearity of the derivatization, so that the current method has the potential to be used for the semi-quantitative analysis of amines.

• Korean Journal of Pharmacognosy
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• v.12 no.3
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• pp.119-124
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• 1981

In our country, in order to cure diseases, a large number of crude drug preparations has been available. Nevertheless, the development of crude drug preparations have been inhibited, because the quality control is not completed so far. Therefore, we have eontinued on studing the quality control method by Zig-zag TLC. profile analysis. The water extract of 'Samyo-Tang' and componental crude drug (Glycyrrhizae Radix, Ephedrae Herba, Armenicae Semen) were developed on Silica gel $60F_{254}\;plate\;(E.\;Merck)$ useing elution solvent. The developed plate were examined useing Dual Wavelength Zig-zag Scanner (Shimadzu). According to the results of the experiment, it could be summarized as follow: 1) Original patterns of TLC profiles of 'Samyo-Tang' componental crude drug and mixing two crude drugs of 'Samyo-Tang' were observed. 2) Original patterns TLC profile of each extract after spraying with 2% ninhydrine were observed. 3) In the extract of addition and subtraction of Ephedrae Herba, peak area of Rf 0.48 and Rf 0.60 were varied quantitatively.

• Journal of Ginseng Research
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• v.11 no.1
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• pp.17-23
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• 1987

A crude alkaloidal fraction from white ginseng (Panax.gilfsertg C.A. Meyer) showed over thirteen Dragendorff positive spots by TLC using eluent of $CHCl_3$/MeOH(10 : 1). TLC pattern of white, red and Sanchii ginseng (P. notoginseng) was mostly not different from each other, but, in American ginseng (P. quinqgtefolium),two spots having Rf 0.71 and 0.68 were not detected. An alkaloid component was isolated from white ginseng and identified as $N_9$-formyl-1-methyl-$\beta$-carboline (mp $174^{\circ}C$, $C_13H_10N_2O$).

• Korean Journal of Pharmacognosy
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• v.7 no.1
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• pp.3-13
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• 1976

The hitherto existing gap in the field of chromatographic methods has been filled by the direct coupling of a suitable oven (TAS-oven) with TLC. The sample to be examined is heated either isothermally or linearly within the temperature gradient of $50{\sim}450^{\circ}C$. The volatile and/or thermolytically evolved substances are fractionated on the TLC-layer and subsequently chromatographed under standard conditions. Transport mechanisms from the sample to the TLC-layer, applications of the TAS-procedure and further developments are discussed. Thermofractography, developed from the TAS-procedure, is demonstrated on different groups of natural substances such as alkaloids, amino acids, nucleic acids. nucleosides, nucleotides, triglycerides and other lipids, pyrone glycosides and aglycon. Experimental work and results on the thermolysis of macromolecular natural and synthetic substances, natural polyphenols, tanning agents and leather and the possibilities of differentiating various lignins, carbohydrate and synthetic polymers are reported. Further, it is shown that classical reactions in the microgram range, e.g. zinc dust distillation, sulphur-and selenium dehydrogenation and catalytic dehydrogenation, can be coupled directly with TLC. Also described is a method which allows to investigate the gaseous compounds evolved during thermofractography in the range of up to $450^{\circ}C$. Thermal procedures coupled with TLC open up the following new possibilities for chemical microanalysis: fractionated separation of distillable and sublimable components, fractionated thermolysis and carrying out of thermal reactions in the ultra micro range.