• Title/Summary/Keyword: TLC

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Plastein formation from sunflower seed protein (해바라기씨 단백질에서 plastein의 합성)

  • Rho, Jae-Mun;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.34 no.1
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    • pp.1-7
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    • 1991
  • Optimum conditions for hydrolysis of sunflower seed by pepsin and for plastein formation by pepsin were determined. The optimum conditions for hydrolysis of sunflower seed were pH 1.5, $45^{\circ}C$, enzyme concentration 2%, substrate concentration 2%, and hydrolysis time 24hr. The optimum conditions for sunflower seed-plastein formation were 50% substrate, pH 4.5, $50^{\circ}C$, 0.25% pepsin and 18hrs reaction time. To verify plastein fromation from concentrated prptic hydrolysate of sunflower seed, thin layer chromatography was performed. The TLC pattern of concentrated peptic hydrolysate of sunflower seed was different from that of its plastein. The TLC pattern of concentrated peptic bydrolysate of sunflower seed and at of its plastein indicated that plastein was different material from the hydrolysate.

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Analysis of Essential Oil from Chrysanthemum sibiricum and the Comparision with Essential Oils from Some Chrysanthemum spp. (구절초의 정유분석 및 동속생약 정유와의 비교연구)

  • Shin, Soon-Hee;Choi, Young-Im
    • Korean Journal of Pharmacognosy
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    • v.13 no.4
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    • pp.153-156
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    • 1982
  • The essential oil fraction of Chrysanthemum sibiricum Turcz. (Compositae) was analysed by means of TLC and GLC. Utilizing silicagel column chromatography, a blue-color compound was isolated, and purified by preparative TLC. The obtain compound had the same Rf-value on TLC and exactly same UV-visible and IR spectra with that of chamazulene isolated from Matricaria chamomilla L. The composition of the essential oil was also compared with those from flowers of Chrysanthemum indicum L. and Chrysanthemum morifolim Ramatuelia.

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Identification of Lanosterol and Ergosterol in Sarcodon aspratus Berk. (S. Ito) (능이버섯에서의 lanosterol과 ergosterol의 확인)

  • 김종봉;박홍덕
    • Journal of Life Science
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    • v.10 no.6
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    • pp.617-620
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    • 2000
  • Methanol extract of Sarcodon aspratus Berk. (S. Ito) was analyzed by thi layer chromatography, gas chromatography and mass spectrophotometer. Nine fractions from primary methanol extract were observed by TLC. Six fractions were observed by the second TLC analysis of the second and the third fractions. 27,28-digydrolanosterol({TEX}$C_{30}H_{52}${/TEX}O), ergost-7-en-3-ol({TEX}$C_{28}H_{48}${/TEX}O) were identified from two fractions of the second TLC analysis by mass spectrophotometer. The molecular weights of 27,28-dihydrolanosterol and ergost-7-en-3-ol were 413 and 400 respectively.

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INTRODUCING tlc_s05: A CODE TO FIT CEPHEID JHK BAND LIGHT CURVES USING A TEMPLATE APPROACH

  • NGEOW, CHOW-CHOONG;KANBUR, SHASHI M.
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.225-227
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    • 2015
  • We introduce a code called tlc_s05, to fit sparsely sampled JHK band Cepheid light curve data with template light curves to derive the mean magnitude. A brief description of the code is provided here. We tested the performance of the code in deriving the mean JHK band magnitudes using simulations, and we found that it is better to observe more than four evenly spaced data points per light curve, which permits tlc_s05 to derive accurate mean magnitudes for Cepheid JHK band light curves.

Normalization and Search of the UV/VIS Spectra Measured from TLC/HPTLC (TLC/HPTLC에서 측정된 자외/가시부 스펙트럼의 표준화 및 검색)

  • Kang, Jong-Seong
    • YAKHAK HOEJI
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    • v.38 no.4
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    • pp.366-371
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    • 1994
  • To improve the identification power of TLC/HPTLC the in situ reflectance spectra obtained directly from plates with commercial scanner are used. The spectrum normalization should be carried out prior to comparing and searching the spectra from library for the identification of compounds. Because the reflectance does not obey the Lambert-Beer's law, there arise some problems in normalization. These problems could be solved to some extent by normalizing the spectra with regression methods. The spectra are manipulated with the regression function of a curve obtained from the correlation plot. When the parabola was used as the manipulating function, the spectra were identified with the accuracy of 97% and this result was better than that of conventionally used the point and area normalization method.

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TLC 이용한 젖산 분리와 정량분석 개발을 위한 연구

  • Jo, Gap-Su;Choe, Mi-Hwa;Ryu, Hwa-Won;Kim, Geun-A;Yu, Seon-Gyun;Kim, Do-Man
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.165-168
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    • 2002
  • TLC(Thin Layer Chromatography) 는 제약산업이나 생화학 연구 그리고 여러 산업 현장에서 널리 이용되는 화학 분석 방법이다 . 본 연구에서는 적은 비용으로도 많은 양의 시료를 신속하게 분리할 수 있는 TLC를 이용하여 유기산인 젖산 (Lactic acid) 을 분리는 전개방법을 개발하였다 . 전개용매는 2 가지 용매 (1) nitroethane nitromethane : ethanol: water: I-propanol = 1 : 2 : 3 : 4 : 5 (v/v/v/v/v), (2) diisopropyl ether. formic acid. water = 90 : 7 : 3 (v/v/v) 를 사용하였다 . 발색시약은 A : bromocresol purple reagent I, B . bromocresol purple reagent II, C : bromocresol green-bromophenol blue-potassium permanganate reagent 를 사용하여 분석하는 방법을 개발하였다 $^6)$. 젖산의 분리는 silica gel TLC plate를 이용하는 경우 , 용매 (1) 에 발색시약 B를 사용했을 때 , 분리 확인이 가장 좋았다.

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A Study on a Passive Sampler for Indoor Formaldehyde Using Methyl Yellow and Methyl Orange Indicator (Methyl yellow와 Methyl orange 지시약을 이용한 실내공기중의 포름알데히드 간이측정기에 대한 연구)

  • Jeong Sang Jin;Jang Jae Chul
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.6
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    • pp.793-801
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    • 2004
  • A passive sampler has been developed for measuring formaldehyde in air using methyl yellow and methyl orange indicator. It is based on the color change of the TLC plate on reaction with formaldehyde. TLC plates impregnated with three kinds of solutions containing methyl orange, methyl yellow, mixing of methyl yellow and methyl orange were shown rectilinear response to formaldehyde concentration at constant exposure time. The color change of impregnated TLC plate with criteria air pollution, such as NO$_2$, SO$_2$, CO, $O_3$ was investigated. The color change of TLC plate with $O_3$ is larger than other gases.

Comparison of Phenolic Components between Korean and American ginsengs by Thin-Layer Chromatography (TLC에 의한 한국산 인삼과 서양상 페놀성 성분의 비교)

  • 위재준;신지영
    • Journal of Ginseng Research
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    • v.22 no.2
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    • pp.91-95
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    • 1998
  • The distribution of phenolic components of Korean ginseng (Panax ginseng KG) and American ginseng (Panax quinquefolium, AG) were compared by thin-layer chromatography (TLC). Silica gel TLC gave 3~4 spots, while $NH_2$ HPTLC 5~6 spots, which were colored by both $FeCl_3$/$K_3Fe(CN)_6$ and Folin-Ciocalteu. The distribution of phenolic components was quite different between KG and AG. Especially, a polyphenol (m.w. 578), which had been isolated from KG by the author, was not found in AG. This result suggests that the polyphenol could be used as an index compound for the differentiation of KG from AG.

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Detection of residual sulfamethazine in serum, urine and muscle of slaughtered pigs (도축돈의 혈청, 뇨 및 근육에서 sulfamethazine 잔류 조사)

  • 추금숙;오언평;최인열;송희종;채효석
    • Korean Journal of Veterinary Service
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    • v.20 no.2
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    • pp.161-168
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    • 1997
  • This study was undertaken to determined the sulfamethazine residues In urine, serum and muscle of slaughtered pigs(n: 230) by the method of TLC, EEC-4-plate and HPLC. 1. Inhibition diameter characteristics of reference bacterial substance by EEC-4-plate method and antibacterial inhibition zone was appeared sulfonamides in BS pH 7.2 2. Residual sulfamethazine was detceted from 7 serum(3.04%) and 10 urine(4.35%) by TLC test and concentration of residues was higher In urine than serum. 3. Residual sulfamethazine was detected from 9 muscle(3.91%) by EEC-4-plate method. 4. Positive samples by TLC test and EEC-4-plate method were exceed 0.1ppm quantitative analysis from 7 muscle(3.04%).

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Hypericin Contents of Hypericum erectum Thunberg (고추나물의 Hypericin 함량)

  • Kim, Sun-Hee;Jung, Young-Jae;Ahn, Jun-Cheul;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.101-104
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    • 2005
  • To explore the Korea native plants to substitute for St. John's wort, which produce hypericin that use commercially for the treatment of mild to moderate depression, hypericin contents of Hypericum erectum and H. ascyron collected in two mountain of Korea were examined. From TLC and TLC-densitometer analysis of hypericin contents and biosynthetic pattern, hypericin was detected in flower and leaf of H. erectum, but not in all organs of H. ascyron. The hypericin content of H. erectum grown 200 m high hill in Mt. Byoung-pung was higher than that of Mt. Ji-ri 500 m and 1300 m high hill. When the seasonal variation of hypericin contents in H. erectum leaf collected from two regions was investigated, leaf collected from both regions on July was higher than other seasons.