• Title/Summary/Keyword: TLC

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Studies on the Analysis of Constituents of Deer Horn(I) -Assay of Trace Elements and TLC Pattern Analysis of Gangliosides- (산지별 녹용(鹿茸)류의 성분분석 연구(I) -미량금속의 정량 및 Gangliosides의 TLC Pattern 분석-)

  • Hong, Nam-Doo;Won, Do-Hee;Kim, Nam-Jae;Chang, Seung-Youb;Youn, Whang-Geum;Kim, Hae-Soo
    • Korean Journal of Pharmacognosy
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    • v.22 no.3
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    • pp.171-182
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    • 1991
  • Several experiments were carried for the purpose of establishing the basis for the quality evaluation of deer horn. Deer horn originated from China, New Zealand, Soviet and Alaska were used as objectives and some constituents involved in deer horn were assayed and compared with one another. Five kinds of trace elements including Ca and Fe were determined by atomic absorbance spectroscopy. The more the region came down from the top of the deer horn, the more the ratio of Ca to Fe content (Ca/Fe) was increased. The same increasing tendancy was observed in the case of ash content. Gangliosides were isolated from deer horn by alumina column chromatography and identified by TLC using gangliosides Type II obtained from bovine brain as the standard material. The TLC chromatograms were scanned by dual wavelength TLC scanner, then those TLC profiles were compared. Deer horn from China and New Zealand were analogous but those from Soviet and Alaska were each distinguished in their TLC profiles.

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NAND-Type TLC Flash Memory Test Algorithm Using Cube Pattern (큐브 패턴을 이용한 NAND-Type TLC 플래시 메모리 테스트 알고리즘)

  • Park, Byeong-Chan;Chang, Hoon
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2018.07a
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    • pp.357-359
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    • 2018
  • 최근 메모리 반도체 시장은 SD(Secure Digital) 메모리 카드, SSD(Solid State Drive)등의 보급률 증가로 메모리 반도체의 시장이 대규모로 증가하고 있다. 메모리 반도체는 개인용 컴퓨터 뿐만 아니라 스마프폰, 테플릿 PC, 교육용 임베디드 보드 등 다양한 산업에서 이용 되고 있다. 또한 메모리 반도체 생산 업체가 대규모로 메모리 반도체 산업에 투자하면서 메모리 반도체 시장은 대규모로 성장되었다. 플래시 메모리는 크게 NAND-Type과 NOR-Type으로 나뉘며 플로팅 게이트 셀의 전압의 따라 SLC(Single Level Cell)과 MLC(Multi Level Cell) 그리고 TLC(Triple Level Cell)로 구분 된다. SLC 및 MLC NAND-Type 플래시 메모리는 많은 연구가 진행되고 이용되고 있지만, TLC NAND-Tpye 플래시 메모리는 많은 연구가 진행되고 있지 않다. 본 논문에서는 기존에 제안된 SLC 및 MLC NAND-Type 플래시 메모리에서 제안된 큐브 패턴을 TLC NAND-Type 플래시 메모리에서 적용 가능한 큐브 패턴 및 알고리즘을 제안한다.

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Temperature field measurement of convective flow in a Hele-Shaw Cell with TLC and color image processing (TLC와 컬러화상처리를 이용한 Hele-Shaw Cell 내부 대류 온도장 측정)

  • Yun, Jeong-Hwan;Do, Deok-Hui;Lee, Sang-Jun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.3
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    • pp.1114-1122
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    • 1996
  • Variation of temperature field in a Hele-Shaw convection cell was measured by using a HSI true color image processing system and TLC(Thermochromic Liquid Crystal) solution. The relationship between the hue value of TLC color image and real temperature was obtained and this calibration result was used to measure the true temperature. The temperature field in the Hele-Shaw convection cell shows periodic characteristics of 45 sec at Ra = 9.3 * 10$\^$6/. The temperature field measurement technique developed in this study was proved to be a useful and powerful tool for analyzing the unsteady thermal fluid flows.

Improved Phosphotyrosine Analysis by TLC and HPLC

  • Song, Young-Me;Yoo, Gyurng-Soo;Lee, Seung-Ki;Choi, Jung-Kap
    • Archives of Pharmacal Research
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    • v.16 no.2
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    • pp.99-103
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    • 1993
  • We describe here the conditions of thin layer chromatography (TLC) and high pressures liquid chromatography (HPLC) to improve the analytical method of phosphotyrosine (p-Tyr) in biological sample. TLC was performed on silica plate with the mixture of propanol and water (2.1 : 1 v/v) as a mobile phase and $R_1$ values were 0.42, 0.39 and 0.33 for phosphotyrosine, phosphothreonine and phosphoserine, respectively. HPLC was performed on $NH_2$ column with a mobile phase of potassium biphosphate solution by UV deterction at 192 nm. The optimum condition of HPLC was obtained at 0.01 M, pH 4.5 with a clear separation within 12 min. These procedures have been applied to the analysis of phosphotyrosine obtained from tyrosine-phosphorylated enolase. Both TLC and HPLC methods were suitable to analyze tyrosine-phosphorylated protein without being affected by contaminants from hydrolysates.

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Densitometric TLC Assay of Aminoglycoside-3'-Phosphotransferase (APH(3')) Produced by E. coli ATCC 21990 (E.coli ATCC 21990이 생산하는 Aminoglycoside-3'-Phosphotransferase(APH(3')) 의 Densitometric TLC Assay)

  • 이정환;김계원;김기태;나규흠;김학주;양주익;김수일
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.372-379
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    • 1991
  • A rapid and simple quantitative assay method for aminoglycoside-3'- phosphotransferase (APH(3')) derived from E. coli ATCC 21990 was developed using the thin layer chromatographic densitometry, 3'-phosphorylated kanamycin B (3'-PKMB), product of APH (3') reaction, was separated from reaction mixtures by developing on the silica gel TLC plate with chloroform-methanol-ammonia water (3:4:3). The quantity of the 3'-PKMB was measured by densitometry after color development by ninhydrin method. Densitometric TLC assay for APH (3') was showed a good quantitative result and reproducibility. Sensitivity of this assay was 1.56 nmol of 3'-PKMB and could be analyzed many samples at same time. This method may be applicable for the analysis of inactivating enzymes of aminoglycoside antibiotics.

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Antioxidant Activity of Amino Acid-Xylose Browning Reaction Products 2. Isolation of Antioxigenic Substrates from Browning Reaction Products by TLC and Dialysis (Amino 산-Xylose 갈변반응 물질의 항산화성 2. TLC와 투석을 이용한 항산화성 갈변물질의 분리)

  • YOU Byeong-Jin;LEE Kang-HO;LEE Jong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.212-218
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    • 1986
  • In order to isolate antioxigenic substrates, the browning reaction products of xylose and various amino acids were analysed by TLC and dialysis. Rf values of browning reaction products of xylose and hydrophobic amino acids separated on silica gel TLC plate were shown in the range of 0.38 to 0.56 and that of basic amino acids was around 0.2. Browning reaction products made from xylose and Trp were separated on TLC into four bands with Rf values of 0.25, 0.55, 0.81 and 0.91 respectively. Among these the bands with Rf values of 0.25 and 0.55 appeared having strong antioxidant activity. The band of Rf 0.55 which showed the highest activity was positive to Prochazka reagent and had an absorption maximum at 275 nm. In dialysis of the xylose-Trp browning reaction products, the undialysed fraction (inner solution) was repsponsible to the antioxidant activity, which was separated into two bands with Rf values of 0.25 and 0.55 on TLC. The inner fractions of the browning products of xylose and His or Arg were also apparent in antioxdant activity.

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Detection of Long Alkyl Esters of Succinic and Maleic Acid Using TLC-MALDI-MS

  • Kim, Hin-Hee;Han, Sang-Pil;Kim, Jeong-Kwon;Kim, Yeong-Joon
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.915-920
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    • 2011
  • Four esters of succinic and maleic acid were synthesized, separated by thin-layer chromatography (TLC), and identified using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). A comparison of matrix materials showed that 2,6-dihydroxybenzoic acid (2,6-DHB) yielded a greater ionization efficiency than 2,5-DHB prior to TLC separation. The location of each ester sample on the TLC plate was estimated by comparing the developed plate with a duplicate plate that had been visualized by immersion in a $KMnO_4$ solution. Generally, mass spectra obtained from the $KMnO_4$-visualized plate were relatively poor. Reproducible mass spectra with high peak abundance were difficult to obtain using the 2,6-DHB matrix from crude synthetic esters extracted from the TLC plates. Significant improvements in both reproducibility and sensitivity were realized by using pencil lead as the MALDI matrix. The current methodology will be beneficial to organic chemists since it can provide a guideline for simple and rapid characterization of small organic compounds.

Comparison of the Microleakage and Shear Bond Strength to Dentine of Different Tricalcium Silicate-based Pulp Capping Materials (Tricalcum-silicate 기반 치수복조제의 미세누출 및 상아질 전단결합강도 비교)

  • Kim, Miri;Jo, Wansun;Jih, Myeongkwan;Lee, Sangho;Lee, Nanyoung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.46 no.1
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    • pp.76-84
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    • 2019
  • This study evaluated the microleakage of three restorative materials and three tricalcium silicate-based pulp capping agents. The restorative materials were composite resin (CR), resin-reinforced glass ionomer cement (RMGI), and traditional glass ionomer cement (GIC) and the pulp capping agents were TheraCal $LC^{(R)}$ (TLC), $Biodentine^{(R)}$ (BD), and $ProRoot^{(R)}$ white MTA (WMTA). Additionally, shear bond strengths between the pulp-capping agents and dentine were compared. Class V cavities were made in bovine incisors and classified into nine groups according to the type of pulp-capping agent and final restoration. After immersion in 0.5% fuchsin solution, each specimen was observed with a stereoscopic microscope to score microleakage level. The crowns of the bovine incisors were implanted into acrylic resin, cut horizontally, and divided into three groups. TLC, BD and WMTA blocks were applied on dentine, and the shear bond strengths were measured using a universal testing machine. The microleakage was lowest in TLC + GIC, TLC + RMGI, TLC + CR, and BD + GIC groups and highest in WMTA + RMGI and WMTA + CR groups. The shear bond strength of BD group was the highest and that of WMTA group was significantly lower than the others.

Rapid Quantitative Analysis of Isoflavones using TLC (TLC를 이용한 이소플라본의 신속한 정량 분석)

  • Kim, Kyung-Seon;Park, Kwan-Hwa;Baik, Moo-Yeol;Kang, Kil-Jin;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.36 no.4
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    • pp.558-562
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    • 2004
  • Conditions for rapid quantification of isoflavones were studied. Rapid and clear separation of isoflavones (genistin and daidzin) was obtained using solvent system of chloroform : methanol : water : acetic acid (60 : 30 : 10 : 0.5, v/v/v/v). Quantification of each isoflavone separated by TLC was conducted by densitometry analysis. Genistin and daidzin were quantified in $0.15-1.80\;{\mu}g/{\mu}L$ range with 99% confidence. Concentrations of isoflavones in soybeans and kudzu roots originated from Korea were determined, and validity of TLC method for quantification of isoflavones was confirmed by comparison with HPLC analysis.