• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 춘계학술대회
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• pp.59-73
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• 2003

• 한국수소및신에너지학회논문집
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• 제34권1호
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• pp.17-25
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• 2023

The purpose of this study was to propose a design that shows optimal performance by changing the geometry of the internal flow path of the receptacle in order to prevent the decrease in flow rate and differential pressure performance due to the application of the receptacle in the hydrogen charging system. To achieve this, 3D computational fluid dynamics simulation was performed for the receptacle, according to the geometry of the flow path inside the receptacle. The pressure results at the inlet and outlet were measured the same as both of N and H₂ in the experiment, and the flow rate of H₂ was 3.75 times higher than that of N₂. In addition, since the flow performance of the receptacle improved under conditions where the flow path was widened, it was confirmed that reducing the diameter of the poppet and the width of the guide are advantageous for improving performance.

• 한국미생물·생명공학회지
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• 제27권2호
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• pp.107-112
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• 1999

A bacterium, which can utilize cyclohexanol as a sole source of carbon and energy, was isolated from sludge in sewage of Ulsan Industrial Complex for Petrochemicals, Korea and identified as Rhodococcus sp. TK6. The growth conditions of the bacteria were investigated in cyclohexanol containing media. The bacteria utilized cyclohexanol, cyclohexanone, cyclohexane-1,2=diol, cyclopentanol, cyclopentanone, and $\varepsilon$-caprolactone but not cyclohexane, cyclohexane-1,2-dione, and cyclooctanone. The bacteria were able to utilize alcohols such as ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 2-methyl-1-propanol, 3-methyl-1-butanol, 2-propanol, and 2-butanol as well as cyclohexanol, organic acids such as adipate, propionate, butyrate, valerate, n-caproate, and 6-hydroxycaproate, and aromatic compounds such as phenol, salicylate, p-hydroxbenzoate, and benzoate as a sole source of carbon and energy. Cyclohexanone as a degradation product of cyclohexanol by Rhodococcus sp. TK6 was determined with gas chromatography.

• 한국관개배수논문집
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• 제1권2호
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• pp.97-103
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• 1994

• 한국조명전기설비학회지:조명전기설비
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• 제22권1호
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• pp.39-43
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• 2008

• Journal of Microbiology
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• 제37권2호
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• pp.102-110
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• 1999

Genetic determinant for the secondary metabolism was studied in heterologous expression in Streptomyces lividans TK-24 using Streptomyces griseus ATCC 10137 as a donor strain. Chromosomal DNA of S. griseus was ligated into the high-copy number Streptomyces shuttle plasmid, pWHM3, and introduced into S. lividans TK-24. A plasmid clone with 4.3-kb BamHI DNA of S. griseus (pMJJ201) was isolated by detecting for stimulatory effect on actinorhodin production by visual inspection. The 4.3-kb BamHI DNA was cloned into pWHM3 under the control of the strong constitutive ermEp promoter in both directions (pMJJ202); ermEp promoter-mediated transcription for coding sequence reading right to left: pMJJ203; ermEp promoter-mediated transcription for coding sequence reading left to right) and reintroduced into S. lividans TK-24. The production of actinorhodin was markedly stimulated due to introduction of pMJJ202 on regeneration agar. The introduction of pMJJ202 also stimulated production of actinorhodin and undecylproidigiosin in submerged culture employing the actinorhodin production medium. Introduction of pMJJ203 resulted in a marked decrease of production of the two pigments. Nucleotide sequence analysis of the 4.3-kb region revealed three coding sequences: two coding sequences reading left to right, ORF1 and ORF2, one coding sequence reading right to left, ORF3. Therefore, it was suggested that the ORF3 product was responsible for the stimulation of antibiotic production. The C-terminal region of ORF3 product showed a local alignment with Myb-related transcriptional factors, which implicated that the ORF3 product might be a novel DNA-binding protein related to the regulation of secondary metabolism in Streptomyces.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.588-591
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• 2000

Sphingoliped 대사의 율속효소인 serine palmitoyl transferase(SPT)와 acid ceramidase의 연구를 위하여 인간의 SPTII 유전자와 acid ceramidase 유전자의 5‘-upstream region을 얻었다. 사람의 대장암세포인 HT29 cell로부터 genomic DNA를 얻고 GenomeWalker kit를 이용하였으며 2690bp의 SPTII promoter와 2028bp 및 1034bp의 acid ceramidase promoter의 fragment들을 얻을 수 있었다. 이들 DNA 조각들을 T7Blue vector에 subcloning하여 sequencing하였으며 이들이 사람의 SPTII 및 acid ceramidase gene의 5’-upstream region임을 확인하였다. 동물세포에서의 promoter activity를 측정하기위하여 firefly luciferase를 reporter gene으로 하는 pGL2-enhancer vector와 pGL2-basic vector에 subcloning하였으며 pRL-TK vctor와 함께 HT29 cell 및 HepG2 cell에 cotransfection 시킨 후 luciferaseg활성을 측정한 결과 같은 양의 DNA로는 사람의 SPTII promoter와 acid ceramidase promoter는 pRL-TK에 비하여 transfection efficiency가 아주 낮았으며 promoter 연구를 위하여는 pRL-TK vector의 양을 1/100으로 줄이는 것이 적당하였으며 HT29 cell보다는 HepG2 cell에 더 높은 발현율을 보였다.

• 한국미생물·생명공학회지
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• 제26권2호
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• pp.110-116
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• 1998

몇 가지 그람양성 세균에서 secY 유전자가 함유된 operon의 구성이 rplO(L15)-secY-adk라는 결과를 토대로 L15와 adk 유전자일부를 primer로 제작하여 Streptomyces lividans TK24에서 secY 유전자가 함유된 1.8kb 단편을 PCR로 증폭하여 얻은 후 secY 유전자를 cloning하였다. 전 단편을 sequencing하여 추론한 아미노산으로 상동성을 조사해 본 결과 Escherichia coli, Bacillus subtilis, Micrococcus luteus, Bacillus licheniformis, Staphylococcus carnosus, Brevibacterium flavum, Streptomyces scabies의 SecY와 각각 46%, 43%, 57%, 44%, 42%, 56%, 90%의 유사성을 보이는 것으로 나타났으며 SecY의 소수성 profile 또한 서로 유사하고 10개의 membrane spanning segment를 동일하게 가지는 것으로 나타났다. 유전자 구조도 다른 그람양성균에서와 같이 L15-SecY-Adk순 이였으며 secY 유전자의 종결코돈과 adk 유전자의 개시코돈이 한 염기를 공유하는 상태의 translational coupling 구조를 하고 있었다. 이와 같이 단백질분비에 관여하는 유전자와 ribosome 구성요소, 그리고 ATP 합성에 관여하는 요소는 세포성장에 상호 연관 작용이 있는 것으로 사료된다.

• 정보처리학회논문지D
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• 제19D권4호
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• pp.271-280
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• 2012

현재 소셜 네트워크 서비스(Social Network Service: SNS)의 이용자 수가 늘어나면서 SNS에서 생성되는 콘텐츠 데이터의 양도 기하급수적으로 늘어나고 있다. 이러한 SNS는 개인의 근황, 관심사를 전달하기 위해 사용하고, 친목도모, 엔터테인먼트, 제품 마케팅, 최신 뉴스 공유, 1인 미디어 등 다양한 목적으로 활용하고 있다. SNS가 스마트폰에서 사용 가능해지면서 사용자들은 언제, 어디서나 실시간으로 사회의 주요쟁점이나 사회구성원들의 주 관심사와 같은 콘텐츠를 기존 미디어 매체보다 빠르게 생성하고 확산시킨다. 기존 웹 콘텐츠 색인 기법은 색인대상이 다양하고 정확성에 중점을 두어 색인하므로 실시간으로 대량 생성되는 SNS 콘텐츠를 색인하는 기법으로 한계가 있다. 이러한 문제를 해결하기 위하여 관계형 DBMS기반 실시간 색인 기법이 있으나 색인대상의 축소와 색인 절차의 복잡성이 높다는 단점이 있다. 따라서 본 논문에서는 실시간으로 생성된 SNS콘텐츠를 색인하기 위하여 NoSQL기반 SNS 콘텐츠 생성시간과 키워드를 각각 색인하는 TK-Indexing 기법을 제안하여 기존 색인 기법의 복잡성을 개선한다.

• Asian Journal of Business Environment
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• 제3권1호
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• pp.29-35
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• 2013

Purpose - objective of this research is to investigate individual, organizational and environmental factors influence tacit knowledge sharing among healthcare professionals. The transmission of Tacit Knowledge is crucial for organizations to ensure that TK will be passed throughout organization, rather than stored in single employee. Research design, data, and methodology - In this study investigate organizational, individual and environmental factors that influence on TK sharing. To test hypothesizes, the survey method was chosen. Sample size was 100 but 74% of questioners returned. Results - The main findings of this research are related to influence of personal, social cultural and behavioral factors on tacit knowledge sharing. According to extracted data all factors have influence on tacit knowledge sharing except Emotional stability that was found to be negatively related to tacit knowledge sharing. That may means anxiety and stress level of workplace applies negative enhance on tacit knowledge sharing. And finally results show that social environment, team oriented culture and organizational commitment have strongest influences on tacit knowledge sharing. Conclusion - the findings of this study shows that personal, social cultural and behavioral factors influence on tacit knowledge sharing. And also indicates that, social and organizational factors enhance strongly on tacit knowledge sharing.