• Title/Summary/Keyword: TIPS

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Effect of Thidiazuron on Callus and Multiple Shoot Formation in Shoot-tip Culture of Hibiscus syriacus L. 'Honghwarang' (Thidiazuron이 무궁화 '홍화랑' 품종의 정단배양으로부터 Callus형성과 Multiple Shoot형성에 미치는 효과)

  • Kim, Eun Kyoung;Yoo, Yong Kweon;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.16 no.4
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    • pp.520-524
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    • 1998
  • This study was carried out to investigate the effect of thidiazuron(TDZ) on callus and shoot primordia formation, to determine the most optimum multiple shoot induction medium, and to obtain the plantlets on solid medium via shoot organogenesis. TDZ 0.01 mg/L in MS medium was most effective on callus formation, and BA 0.1 mg/L was most effective on shoot growth, while TDZ 0.01 mg/L was most effective on callus formation. TDZ 0.001 mg/L was most effective in shoot primordia formation. Shoot tips were cultured with TDZ 0.01 mg/L for 8 weeks and induced callus was transferred to regeneration medium containing TDZ 0.001 mg/L. After 4 weeks induced shoot primordia were resubcultured at growth regulator-free medium for 4 weeks. The induced multiple shoots rooted more efficiently at NAA 1.0, 5.0 mg/L, or IBA 5.0 mg/L.

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A Study on the Pattern of Growth Process of the Parents of Children with Developmental Disabilities based on Online Parental Community (발달장애아동 부모의 온라인 공동체 상호작용과 성장과정 유형에 관한 연구)

  • Lee, Kyungah;Kim, Sungchun;Chang, Haelim;Lee, Eunjoung
    • Korean Journal of Social Welfare
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    • v.66 no.4
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    • pp.181-205
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    • 2014
  • The purpose of this study is to analyze the process of growth of parental empowerment of the family of the children with development disabilities in an online parental community. For this purpose, 250 posts were selected from a web-forum of an online-community(internet CAFE) for patients with developmental disabilities in a Korean portal site. In addition, the selected posts were analyzed based on the grounded theory method. The results showed that the parents with high risk children for developmental disabilities interacted with each other in short answers, self-addressing, and discussion type interactions under the causal condition in which the subject parents were in need of help and sympathy. The factors that significantly affected the focus event, which is the interactive communication between the posters of the original threads and replies, included the moderating conditions based on whether the conversation was respectful, friendly, or for general evaluation, as well as the contextual condition of exclusive attitudes. The strategies of the interactions were composed of two categories of self-reflections and sharing through a human relationship. The results of these interactions were either further interactions (sharing) or shying away. With regard to the process of reinforcing the collective empowerment of the family, the 'determination,' 'tips,' and 'empathy' models were used for the explanation of the process. Lastly, we discovered that trust, support, continuous interactions, specific and practical information, as well as provision of diversified perspectives through collective experiences are necessary to achieve such improvement of collective family empowerment.

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A New Species of Cellular Slime Molds from Korea, Dictyostelium flavidum sp. nov. (한국산 세포성 점균의 1 신종)

  • 홍정수
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.197-203
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    • 1992
  • During the study of the distribution of cellular slime molds in Halla mountain of Korea, a new yellow-pigmented Dictyostelium was isloated. This exibited several distinctive features which differed from the published species, and was designated as a new species, Dictyostelium jlavidum sp. n. Hong et Chang (Type strain HL-1). It was cultivated at $20-22^{\circ}C$ on weak nutrient agar media, 0.1 L-P in association with Escherichia coli. Sorocarps were 4-10 mm or more in length, conspicuously yellow throughout or with sori, typically solitary, unbranched or sparsely and irregularly branched. Sorophores were strongly tapered from bases to tips. Bases were typically well-formed disks in form or conically expanded. Sori were yellow to yellowish in color, and the pigmentation intensified with age. Spores were long and thin elliptical, mostly $4.8-9.6{\times}1.9-3.8\;(ave.\;7.3{\times}2.8)\;\mu\textrm{m}$, L/B index about 2.4-2.8, without polar granules.anules.

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EFFICIENCY OF ULTRASONIC ROOT-END RETROGRADE CAVITY PREPARATION AND ITS INFLUENCE ON TOOTH STRUCTURE (초음파기구의 치근단 역충전와동형성 효율 및 치질에의 영향)

  • Lee, Jae-Whan;Kim, Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.22 no.2
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    • pp.546-559
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    • 1997
  • The purposes of this study were to evaluate the efficiency of cavity preparation and to determine the incidence of tooth crack when root-end retrograde cavity preparation was done with ultrasonics. 91 distobuccal root-ends of extracted human maxillary first molars were cut by 3 mm perpendicularly to the long axis of tooth using a slow speed diamond saw, retrocavities were prepared using a slow-speed no. 2 round bur as controls, and stainless steel ultrasonic tips of power settings of 1 through 10 as experimentals. Time consumed and the number of strokes used for the cavity preparation were measured and evaluated, and the incidence of tooth cracks was observed under a stereomicroscope. The results were as follows : For the retrograde cavity preparation, time and number of strokes used were decreased as the ultrasonic power setting increased (p<0.001). High power setting of ultrasonics induced significantly more tooth cracks than did the slow-speed bur or low- and medium power setting of ultrasonics (p<0.05). Teeth with previous crack induced significantly more tooth cracks than those without previous one when high power setting of ultrasonics were used for the retrograde cavity preparation (p<0.001). Teeth with initial apical canal size of no. 10 induced significantly more crack than did those with size of no. 15 when low power setting of ultrasonics were used for the retrograde cavity preparation (p<0.05).

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Shoot Primordium Culture for Multiplication of Carrot (당근의 다량증식을 위한 순원기 배양)

  • 서호범;이수성
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.2
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    • pp.93-97
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    • 1999
  • Shoot tips with 2 leaf primordia were cultured to induce shoot primordia in MS liquid medium supplemented with several concentrations of BA and hIAA under the conditions of 10,000 lux illuminations for 24 h and of vertical shaking of 2 rpm in carrot. Two F$_1$ hybrids and two male sterility lines were used. Shoot primordia were only induced in the medium supplemented with 2.0 mg/L of BA and 0.2 mg/L of NAA. Genotypic specificity and seasonal effect of donor parents on shoot primordia induction were not observed and average 15-20% of the planted dornes developed to shoot primordia. The induced shoot primordia were successfully propagated by subculture in the same medium. However, they were grown into three different types during multiplication, that is, the type with multiple small shoots on the surface, the type of without any shoot, and the type of callus. Shoot primordia clusters with small shoots on the surface differentiated multiple shoots successfully in 1/2 MS solid medium supplemented with 0.2 to 1.0 mg/L of IAA and 0.2 to 1.0 mg/L of kinetin. New shoot primordia with small shoots were well formed when pieces bigger than 2 mm in diameter of the out layer of the shoot primordia cluster with small shoots were subcultured. No differences of multiplication and shooting ability and chromosomal variation of shoot primordia were observed until the 13th sub-culture.

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Current Tendency of Middle School Students to Get the Food and Nutrition Information from the Internet Web Site (중학생들의 식생활 정보 Internet Site 이용 현황)

  • 고봉경;윤진숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.1
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    • pp.102-108
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    • 2003
  • This study investigated the current tendency of the middle school student's use of the food and nutrition information from the internet web site. The research employed web survey for the national middle school students. Middle school students usually searched the internet information to do homework and to solve the own curiosity. Forty percent of respondents had experience to get food and nutrition information from tile internet, and 74% of them were girl. Cooking method and world cooking exploring were the most wanted information among food and nutrition information. Professional cooking site and tips of food and nutrition from study aiding sites and monthly magazine sites were tile resources of food and nutrition information. The mai or reasons for searching the food and nutrition information were to know the cooking method and out of curiosity. The most unsatisfied problems were unnecessary information, too much advertisement and too difficult contents when the users approached the site to get the food and nutrition information. Students who have been not interested in and had no homework about food and nutrition, had no visiting experience of web sites for food and nutrition information. Therefore, to provide food and nutrition information for teenagers through internet, web sites were developed to have easy contents linked to study aiding site or other sites that teenagers frequently visit. Since teenager's interest about health issues is not as high as adult's one, the contents of sites must be easy for teenagers to follow and fun, however, should be based on scientific facts on food, nutrition and disease.

Seasonal Variation of Arginine in Buds of Pinus radiata in Relation to Flower Initiation (Pinus radiata 소나무의 정아내(頂芽內) Arginine함량(含量)의 시기적변화(時期的變化))

  • Hong, Sung Ok;Sweet, Geoffret B.
    • Journal of Korean Society of Forest Science
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    • v.33 no.1
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    • pp.59-67
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    • 1977
  • Seasonal changes in free arginine concentration in Pinus radiata buds were studied in four clones from May, 1974 to June, 1975. The buds were from the tips of first order branches which had flowered the previous year, and the significant findings were as follows: (1) In general, arginine concentration in the buds of the four clones showed the highest peaks from December to March, a period spanning the time of flower initiation, and the lowest levels in Angust and September. (2) The timing of individual arginine peaks during the period of flower initiation matched roughly the timing of initiation of several female cone clusters, as determined from Clone 7 which characteristically initiates three cycles of female cones on its leading shoot. (3) The heavy-flowering clones showed higher arginine concentration than ther poorer flowering ones, especially at the time of flower initiation.

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Feeding Behavior of the Small Brown Planthopper, Laodelphax striatellus (Hemiptera: Delphacidae) on Rice Plants Based on EPG Waveform, Honeydew Excretion, and Microsection Analysis (EPG 파형과 감로 분비, 미세절편 관찰로 해석된 애멸구의 벼 섭식행동)

  • Seo, Bo Yoon;Kwon, Youn-Hee;Jung, Jin Kyo;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.351-358
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    • 2016
  • Consistent with a previous study on the brown planthopper Nilaparvata lugens (BPH) (Seo et al., 2009), we identified seven distinct EPG waveforms (np, L1, L2, L3, L4-a, L4-b, and L5) in adult female Laodelphax striatellus (SBPH) that fed on rice plants, by using the direct current electrical penetration graph (DC-EPG) system. The shape of waveforms and the pattern of occurrence of each waveform of SBPH were very similar to those of BPH. L3 and L4-a always occurred prior to L4-b. Periodical honeydew excretion was observed in L4-b only. Microsection observation following laser stylectomy revealed that the tips of SBPH stylets severed in L3, L4-a, and L4-b were commonly located in or near the phloem region of rice plants, but were located in the xylem in L5. Plant sap flowed from the stylets severed in L4-b only, and its main carbohydrate component was detected as sucrose by HPLC analysis. These results and the patterns of EPG waveform progress in SBPH suggested that feeding activities on rice plant tissue were relevant to each EPG waveform. L1 and L2 corresponded to the initiation of stylet penetration and stylet movement with salivation on the outside of the vascular bundle. L3 and L4-a were related to feeding activities within the phloem region in preparation for phloem sap ingestion. L4-b was closely associated with phloem sap ingestion, and L5 corresponded to xylem feeding behavior.

Change of Lumbar Spine and Hip Joint Flexion Angles During Forward Bending of the Trunk Using Manual Facilitation and a Stick (도수 촉진과 막대를 이용한 몸통의 전방 굴곡 운동에 따른 요추와 고관절 굴곡 각도의 변화)

  • Choung, Sung-Dae;Park, Kyue-Nam;Hong, Ji-A;Cho, Min-Sue;Son, Dong-Hwi;Cynn, Heon-Seock
    • Physical Therapy Korea
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    • v.18 no.1
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    • pp.57-63
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    • 2011
  • The purpose of this study was to identify the effects of manual facilitation and a stick on lumbar and hip joint flexion angles in subject with lumbar flexion syndrome during forward bending from a sitting position. Fifteen subjects with lumbar flexion syndrome were recruited for this study. As a pretest, all subjects performed three repetitions of bending the trunk forward until the tips of their fingers touched the target bar. After this pretest, the subjects practiced the forward bending of the trunk 10 times, using either manual facilitation or a stick. Then, as a posttest, all subjects repeated the pretest procedure. The flexion angles of lumbar spine and hip joint during forward bending in a sitting position were measured using a three-dimensional motion analysis system. A paired t-test was used to determine the statistical differences between pre-test and post-test flexion angles and pre- and post-test flexion angle differences between forward bending with manual facilitation and forward bending with a stick. The level of statistical significance was set at p=.05. The results of the study showed that the angle of the lumbar flexion decreased significantly and the bilateral hip flexion angle increased significantly when performing forward bending with stick and manual facilitation. Furthermore, the angle of lumbar flexion decreased significantly and the angle of bilateral hip flexion increased significantly in forward bending with a stick compared to forward bending with manual facilitation. The findings of this study indicate that both forward bending with manual facilitation and sticks could be used to prevent excessive lumbar flexion and increase hip flexion, and that forward bending with a stick is more effective than forward bending with manual facilitation for inducing lumbar spine and hip joint angle changes.

Characteristics of Marriage Immigrants' Acculturation Stage and the Source of Support: With an Emphasis on Filipino Marriage Immigrants' Family Life Culture in Korea (결혼이주여성의 한국가정생활 문화적응 단계별 특성 -필리핀 결혼이주여성을 중심으로-)

  • Hong, Dal-Ah-Gi;Chae, Ock-Hi;Han, Eun-Jin;Song, Bok-Hee
    • Journal of Family Resource Management and Policy Review
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    • v.16 no.4
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    • pp.1-22
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    • 2012
  • The purpose of this study is to determine the characteristics of Filipino marriage immigrants' acculturation stages in regards to their Korean family life culture and to identify the source of the support for each stage, hence to provide information for educational programs that would promote successful acculturation for each stage. The following findings have been obtained through in-depth interviews with 18 female Filipino immigrants to Korea. In a range of obstacles from the Honeymoon Stage to the Confusion stage, the first are the language and the aspects of the food/cooking/ingredients/diet that are different from their own culture. Especially, pregnancy/childbirth is a major change in one's life and the biggest challenge in the acculturation process. As food and cooking are the first change that the immigrants have to face and get accustomed to in the early stage of their Korean life, the food culture is rather easier for the immigrants to get accustomed to than other parts of Korean life. From the Honeymoon Stage to the Harmony Stage, the immigrants make efforts to help their family in the home, while they look to their future in their children during the Autonomy Stage. Regardless of how long they have been in Korea, from the Honeymoon Stage to the Autonomy Stage, the immigrants have a hard time with the patriarchal environment in Korea due to the bilateral nature of kinship in the Philippines. Secondly, the immigrants receive the most support from their husband, family, and the tutors in Korean culture, while their mothers-in-law are the main source of the support for the Korean diet. At the Confusion Stage, the immigrants start visiting the regional multicultural family support centers and get help from the friends they meet there while depending on the TV for cooking tips. From the Harmony Stage, they may seek a job through the community network with their own effort and their children's help. In the Autonomy Stage, they are concerned about their children rather than their own parents, and they find their own identity as a Korean and realize that their effort is important.

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