• 생명과학회지
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• 제19권9호
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• pp.1289-1293
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• 2009

원암단백질로 알려진 Human cervical cancer oncogene (HCCR)은 발암억제 단백질인 p53과 작용하여 다양한 암조직에서 암의 유발을 촉진한다. 그러나, 아직 정확한 발암 유도기전이 알려져 있지 않다. 이러한 의문을 해소하기 위한 일환으로 본 연구에서는 HCCR의 발현이 어떻게 조절되는지를 조사하였다. 이를 위해 먼저 HCCR 5' 쪽의 promoter 영역을 분리하여 Luciferase assay법을 이용하여 K562, HEK293, A549 세포에서 분석하였고, Promoter의 -370에서 -406사이 36bp의 첨가로 promoter활성이 의미 있게 억제됨을 관찰하였다. 또한, 36 bp만을 포함하는 probe를 이용한 mobility shift assays (EMSA)에서 핵단백질이 결합함을 관찰하였고, 컴퓨터를 이용한 분석에서 TG-interacting factor (TGIF)에 대한 consensus sequences 존재함을 관찰하였다. TGIF 만을 포함하는 probe (TC)와 돌연변이를 유발한 probe (mTG)를 이용한 EMSA에서 이 자리에 TGIF가 결합함을 보였다. 또한, TGIF 자리에 돌연변이를 유발하면(pGL3-mTGIF) 발현의 억제가 회복됨을 관찰하였다. 본 연구에서는 HCCR promoter의 특성을 분석하였고, 이 과정에서 -390에서 -366 사이에 TGIF 전사인자가 결합하여 전사활성을 조절함을 증명하였다.

• IMMUNE NETWORK
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• 제9권6호
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• pp.248-254
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• 2009

[ $TGF-{\beta}1$ ]is well known to induce Ig germ-line ${\alpha}$ ($GL{\alpha}$) transcription and subsequent IgA isotype class switching recombination (CSR). Homeodomain protein TG-interacting factor (TGIF) and E3-ubiquitin ligases TGIF interacting ubiquitin ligase 1 (Tiul1) are implicated in the negative regulation of $TGF-{\beta}$ signaling. In the present study, we investigated the roles of Tiul1 and TGIF in $TGF{\beta}1$-induced IgA CSR. We found that over-expression of Tiul1 decreased $TGF{\beta}1$-induced $GL{\alpha}$ promoter activity and strengthened the inhibitory effect of Smad7 on the promoter activity. Likewise, overexpression of TGIF also diminished $GL{\alpha}$ promoter activity and further strengthened the inhibitory effect of Tiul1, suggesting that Tiul1 and TGIF can down-regulate $TGF{\beta}1$-induced $GL{\alpha}$ expression. In parallel, overexpression of Tiul1 decreased the expression of endogenous IgA CSR-predicitive transcripts ($GLT_{\alpha},\;PST_{\alpha},\;and\;CT_{\alpha}$) and $TGF{\beta}1$-induced IgA secretion, but not $GLT_{\gamma3}$ and IgG3 secretion. Here, over-expressed TGIF further strengthened the inhibitory effect of Tiul1. These results suggest that Tiul1 and TGIF act as negatively regulators in $TGF{\beta}1$-induced IgA isotype expression.

• 월간 기계설비
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• 통권246호
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• pp.86-87
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• 2011

`TGIF'라고 하면 무엇이 떠오를까? "유명한 패밀리 레스토랑"아니면 "서양 사람들이 주말 잘 보내라고 하며 쓰는 말인 Thank God It's Friday"정도일 것이다. 필자 역시 얼마 전까지 그랬다. 그런데 그것이 엄청난 시대의 변화 밑에서 잠자고 있다는 뜻임을 최근에 알았다. 그렇게 말하면 너무 가혹하고 무례한 진단일까? 하지만 이 시대에 가장 화두가 되고 있는 트렌드 하나를 놓치고 있는 것만은 확실하다. 2011년을 뒤집어 놓을 SNS(Social Network Service)에 관하여 잠시 후 본문에서 간단히 살펴보자.

• 한국조리학회지
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• 제13권1호
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• pp.24-40
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• 2007

This study investigated an effective countermeasure of each restaurant under the present market conditions by grasping consumers‘ brand recognition and preferences over six restaurants in the metropolitan area centering around Seoul and comparing and analyzing selection properties that satisfy customers. The survey used a self-recording method that respondents write in the answers in person, and its subjects were the customers of restaurants in the metropolitan area from July to August, 2006. We made the MDS(multidimensional scaling) analysis of the available data from 408 respondents, using the SPSS statistical analysis program. The result was as follows. First, there were intensely competitive relationships between Nolboo Bossam and Wonhalmeoni Bossam, and between Outback Steakhouse and TGIF. Marche and Bennigan’s, however, didn‘t have a competitive relationship but kept a complementary one. Second, Wonhalmeoni Bossam was most favorably commented upon in its facilities, atmosphere, location, services, convenience(business hours, parking, transportation), recognition(popularity, fame, advertisement), sanitation and cleanliness, reasonable prices (food and drinks), etc., and then came Nolboo Bossam, TGIF, Outback Steakhouse, Bennigan’s, and Marche in that order. Third, in case of selection properties on taste of food, TGIF was estimated as the best, and then came Outback Steakhouse, Bennigan's, Marche, Wonhalmeoni Bossam, and Nolboo Bossam.

• 한국조리학회지
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• 제10권1호
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• pp.153-166
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• 2004

This study analyzed the positioning of family restaurants by image factors, as a result, customers divided into 5 groups from 7 restaurants. All customers realized similar images from subject restaurants, which told that those restaurants are within an intense competition. Strong points of each restaurant are as follows by factors; Outback and TGIF was confidence, Sizzler and Outback was response, Outback and Skylark was type, Bennigan's and TGIF was reliability, Bennigan's and Outback was reactivity. Weak points each restaurant are as follows by factors; Bennigan's and TonyRoma's were confidence and response, TonyRoma's and Marche was type, Sizzler and TonyRoma's was confidence, Sizzler and TonyRoma's was reactivity. The analysis on each restaurants' images by factors told that response and reactivity were above the average, confidence and reliability were around the average, type was the lowest value. It told that customers didn't give a high rating toward restaurants' food flavor, freshness, cleanliness, therefore restaurants need pay more attention to positioning strategies, Instead, customers gave a high rating toward service, accessibility, menu-variety, and parking-convenience, therefore, continuous improvement is required.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제12권1호
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• pp.53-59
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• 2012

As the Privacy Act is in force in Korea, the subject of protection responsibility is increased, and continuous efforts are made to protect privacy in overseas countries, as can be seen by standard drafts related to privacy protection. However, the reality is that a formal privacy manual or guidelines are insufficient to help cope with the rapid changes and privacy leak caused by TGIF(Twitter-Google-iPhone-Facebook) these days, and practical effects cannot be expected, even though measures are taken. This paper propose a standard format for satisfying the ISO/IEC 29101 "Privacy Reference Architecture" and shows an implementation example for equipping with forensic readiness capturing indications of the incident rapidly and coming up with an effective counter measure when privacy information is disclosed.

• 한국식생활문화학회지
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• 제26권2호
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• pp.142-150
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• 2011

This study investigated and compared the brand personality of domestically run family restaurants with quick service restaurants. Information was elicited from respective patrons using self-administered questionnaires which asked questions on recognizable brand characteristic and attributes of each restaurant through image maps. Data from the self-administrated questionnaires were collected and analysed using comparative statistical analysis, including frequency, t-test, chi-square, factor analysis, reliability analysis and correspondence analysis. Overall, patrons identified 5 factors which they used to rank individual restaurants; sophistication, competence, likeableness, trendiness, and ruggedness. Overall results showed the family restaurants that scored highly on likeableness and trendiness were Outback and TGIF. Furthermore, Vips was the only family restaurants that scored highly in ruggedness. In relation to quick service restaurants, Lotteria scored highest for competence while McDonalds and Burger King scored best for sophistication. Limitations and future research directions are also discussed.

• 한국조리학회지
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• 제16권1호
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• pp.38-49
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• 2010

본 연구는 패밀리 레스토랑 인기 메뉴로서의 폭립 메뉴에 대한 품질 개선을 위한 것으로서, 현재 패밀리 레스토랑에서 판매되고 있는 폭립 소스의 이화학적, 물리학적 특성 및 고도로 훈련된 관능평가요원을 대상으로 한 관능검사를 실시하여 관능적 특성과 관능검사를 통한 폭립 소스와 폭립간의 관계에 대하여 알아보고자 하였다. 연구 결과 TGI.F 레스토랑에서 구입한 폭립과 폭립 소스의 관능적 선호도가 가장 높은 것을 알 수 있었는데, 폭립과 함께 제공되는 폭립 소스는 다른 매장소스 시료들에 비해서 짙은 색을 띠며, 짠맛은 다른 시료에 비하여 다소 염도가 높은 맛을 선호하는 것으로 보여졌으며, 단맛은 당도의 차이를 통하여 본 결과 당도가 낮은 것을 선호하는 것으로 나타났다. 점도는 진하지 않은 농도의 점도를 선호하는 것을 알 수 있었다. 폭립과 함께 폭립 소스를 함께 시식한 경우는 단맛, 짠맛, 신맛, 매운맛, 점도, 종합적인 기호도 등에서 차이는 보이는 것으로 나타났다. 또한 폭립 소스와 함께 폭립을 제공한 형태에서의 점도에 대하여 폭립 소스만을 제공한 형태에서의 상관관계를 살펴 본 결과, 폭립과 함께 제공되는 소스가 폭립과 밀접한 관계가 있는 것으로 보여지며, 이에 따른 폭립 소스의 다양화가 이루어진다면 패밀리 레스토랑에서의 폭립 메뉴의 선호도는 높아질 것으로 사료된다.

• 산경연구논집
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• 제10권1호
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• pp.59-67
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• 2019

Purpose - The Millennial Generation, which grew in the wake of the spread of the Internet and rapid changes in the media environment, is rapidly moving from the traditional broadcasting environment to the Internet-broadcasting environment in terms of content acceptance. With the emergence of UGC (User-generated content), the change in the status of single-person content creators enables the growth of multi-channel networks (MCN), a new content-distribution platform and an agency concept for single creators. Youtube-based MCN produces multiple single star producers and casts and provides its own video series through Youtube. It is also emerging as a major M&A target for global media providers in terms of providing content to a wide range of consumers with the same interests and consumption characteristics. In addition, for the Millennials generation, which are part of their lives, MCN is becoming the most suitable media for TGIF (Twitter, Google, i-phone, Facebook). Accordingly, this study defines newly emerging MCNs and analyzes the factors for accepting MCN-produced content based on the push-pull-mooring (PPM) model. Research design, data, and methodology - An empirical analysis is performed through a questionnaire survey. For this purpose, 204 people who have experience of watching MCN were studied. Collected data is processed through analysis of a structural equation model using R to test the hypothesis. Results - For the MCN service to become an alternative to existing media, it is necessary to continuously promote cultural diversity and diversity of attempts that conventional media cannot provide. It is the attractiveness of the alternative that has the greatest influence on the intention to switch to a MCN service. When we look at MCN content so far, certain patterns such as game progress, introduction, food, and chat rooms have already appeared. We need to overcome this and develop a completely new conceptual content that we have never seen before. This requires a more generous viewer perception of the topics covered. For diversity, linguistic and verbal violence should be tolerant in common sense to provide a foundation for securing cultural diversity. Conclusions - In this study, we tried to develop a comprehensive approach to the substitution effect of MCN. In terms of academic achievement, the PPM model is used to enhance the utilization of media and broadcasting. Practical implications are to provide an analytical framework for verifying alternative or complementary effects when viewers switch to MCN.