• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.327-332
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• 2016

The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average $F_{is}$ value for the breeds ranged from -0.044 (ETH225) to 0.180 (SPS115), while the estimated average $F_{st}$ value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy.

• 대한지역사회영양학회지
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• 제6권2호
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• pp.162-171
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• 2001

This study was performed to investigate the relationship between obesity, health-related lifestyle, and dietary intake and serum lipid level in 96 male university students. Health-related lifestyle factors were obtained from questionnaires. Dietary intakes were evaluated with one-day 24-hr recall and two-day dietary record. Anthoropometric data were recorded and serum cholesterol and triacylglycerol concentrations were measured. 21.9%, 36.5%, 36.5%, and 6.2% of the subjects had levels beyond the normal range in serum total cholesterol(TC), HDL-cholesterol(HDL-C), LDL-cholesterol(LDL-C), and triacyglycerol respectively, and 57.3% of the subjects had more than one hyperlipidemic factor. TC was correlated positively with BMI(p〈0.01), waist length(p〈0.05), hip length(p〈0.05), and the amount of smoking(p〈0.05). HDL-C was correlated negatively with BMI(p〈0.05) and hip lenghth(p〈0.05). LTD-C was correlated positively with BMI(p〈0.01), water length(p〈0.05), hip length(p〈0.01), and coffee consumption(p〈0.05). TG was correlated positively with waist length(p〈0.01), waist-to-hip ratio(p〈0.05), and amount of smoking(p〈0.01) and negatively correlated with frequence of exercise(p〈0.05). Among nutrient intakes, only the ratio of protein to energy was correlated negatively with TC(p〈0.05). Logistic regression analysis revealed that BMI, waist length, hip length, waist-to-hip ratio, and amount of cigarette smoking were associated with an increased risk of hyper-TC. BMI, waist length, and hip length were associated with an increased risk of hypo-HDL-C. BMI and coffee consumption were associated with an increased risk of hyper-LDL-C. Amount of cigarette smoking was associated with an increased risk of hyper-TG. In conclusion, a high prevalence of hyperlipidemia in subjects was observed. Also obesity, smoking, and coffee consumption were observed to be highly with the risk of hyperlipidemia in subjects. These findings imply that these factors should be primarily considered in planing the nutrition education program for the prevention of cardiovascular disease in male university students.

• Toxicological Research
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• 제21권1호
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• pp.1-14
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• 2005

Transgenic mouse models have been introduced and accepted by regulatory bodies as an alternative to carcinogenicity assay models to predict and evaluate chemical carcinogens. The recent research outcomes in transgenic mouse models have made progressive advances in the understanding of chemical carcinogenesis and the evaluation of potential human carcinogens. However, these models still remain to be insufficient assay systems although the insufficiencies have been recognised and are being resolved. Based on up to date information from literature, this review article intends to understand currently accepted transgenic mouse models, issues arising from study design, interpretation of the study, results of validation project and their cancer prediction rate, and further perspectives of cancer assay models from the regulatory view point.

• Journal of Nutrition and Health
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• 제21권1호
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• pp.23-35
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• 1988

This study was designed to find out the effects of aerobic exercise on lipid and Ca metabolism in seven healthy college women, aged 20 to 22 years. Metabolic studies were conducted before and after a 10-weeks exercise period, during which subjects participated in the planned aerobic dance program every day except Sunday. The initial mean time engaged in the exercise was 28 minutes and it was gradually increased up to 45 minutes around the middle point of the exercise period. During both of metabolic study periods, the subjects ate experimental diets which supplied about 120g of protein and 600mg of Ca daily and during the rest of the experimental period they ate their usual diets. The use of alcoholic beverages and drugs were prohibited. The results were summarized as follows. 1) The effect of exercise on lipid metabolism. (1) Mean body weight decreased significantly after exercise(p<0.01) and it did not show a significant decline in skinfold thickness and total body fat contents. (2) Serum total cholesterol level decreased significantly after exercise(p<0.05) and TG level also tended to be lower than that of pre-exercise period. Exercise did not exert any influence on the level of serum HDL-cholesterol in this study. (3) Exercise did not alter total lipid content in feces and apparent lipid absorption rate. 2) The effect of exercise on Ca and P metabolism. (1) After exercise, focal Ca excretion was slightly reduced, however, urinary Ca excretion was not significantly changed. In the results, a slight increase was shown in body Ca retention after exercise. (2) Exercise tended to increase urinary P excretion, but neither P balance nor fecal excretion was significantly changed after exercise. (3) Bone mineral content was not affected by exercise. In summary, aerobic exercise decreased total cholesterol and TG level in serum and tended to increase body Ca retention. With the results, it can be concluded that the additional physical activities beyond the normal daily life in college women might prevent some degenerative diseases-suchas atherosclerosis and osteoporosis.

• Asian-Australasian Journal of Animal Sciences
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• 제19권2호
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• pp.236-244
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• 2006

This study was designed to determine the effects of daidzein (DE) on hepatic lipid metabolism in chicks fed with low protein (LP) diet based on casein. In experiment 1, the male chicks were fed with one of the three levels of dietary protein containing 10.95%, 21.9% and 43.8% protein content for 2 days. In experiment 2, the chicks were fed one of the three levels of protein with or without DE at 1,000 mg/kg diet for 2 days. Experiment 3 was conducted to compare DE (LP+DE) with estradiol (LP+E2) in chicks fed with LP diet for 7 days. Plasma lipid profiles, hepatic lipid profiles, activities of hepatic malic enzyme and isocitrate dehydrogenase (ICDH) were measured. Transcriptions of hepatic fatty acid synthase, apolipoprotein-B (APO-B), and fructose bisphosphatase mRNA were measured by RT-PCR. Increasing dietary protein levels markedly decreased the concentrations of plasma triglycerides, hepatic total lipids, hepatic TG, and the mRNA transcriptions while the increased dietary protein levels increased hepatic ICDH activities in experiment 1. In experiment 2, the effects of dietary protein levels on blood and hepatic lipid content were more prominent than those of the additional DE. Interestingly, plasma TG levels were affected by DE supplementation (p<0.05). In experiment 3, DE inhibited APO-B mRNA expressions and stimulated the accumulation of lipid in the liver through mechanisms different from E2. In this study, we demonstrate that DE has beneficial effects on blood lipid profiles, but that it inhibits APO-B mRNA transcription and aggravates the fatty liver induced by LP diet in chicks.

• 공업화학
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• 제21권5호
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• pp.581-585
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• 2010

산업폐기물 중의 하나인 무기 금속화합물, Alnico를 1,3,5-trimethylcyclotrisilazane으로 표면 처리하여 충전제로 하고 EPDM에 maleic anhydride (MA)를 용융 중합법으로 그라프트시켜 제조한 말레화 EPDM과 ${\alpha},{\omega}$-bis(3-aminopropyl)polydimethylsiloxane을 공중합시켜 제조한 말레화 EPDM-polydimethylsiloxane공중합체(MEPDM-PDMS)와 EPDM 및 표면처리된 Alnico를 compounding하여 composite rubber를 제조하였다. EPDM/Alnico/MEPDM-PDMS 방진고무는 MEPDMPDMS와 EPDM을 중량비로 1 : 10으로 하고 또한 충전제의 함량을 EPDM에 대하여 25, 50 phrs로 그리고 개시제로 dicumyl peroxide (DCP)를 첨가하여 compounding시킨 다음 유압 고온 프레스로 가교시켜 제조하였다. 제조한 composite rubber의 열적특성을 측정한 결과 MEPDM-PDMS를 첨가하여 제조한 시료의 Tg가 EPDM만으로 제조한 고무보다 약간 낮은 $-33^{\circ}C$ 부근으로 나타났고 DMA의 측정결과 손실계수 tan ${\delta}$는 MEPDM-PDMS을 혼합한 경우가 더 크게 나타나는 것으로 보아 방진 특성이 우수함을 확인하였다.

• 한국식품영양과학회지
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• 제27권2호
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• pp.338-343
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• 1998

Artemisia iwayomogi(A. iwayomogi) is a major edible vegetable in Korea. A iwayomogi containing a strong bitter taste, particularly, is known to manifest an effect on cough, abdominal pain, indigestion and bleeding. In this experiment, ethanol extract of A. iwayomogi(50mg/kg body weight) was fed to growing male Sprague-Dawley rats for four weeks in order to examine the lipid lowering effect of A. iwayomogi in the live and serum. High fat diet included 10% lard, 1% cholesterol and 0.25% sodium cholate. The administration of A. iwayomogi extract decreased serum cholesterol compared with the control. At the same time, serum triglyceride(TG) was decreased to 25%. Although atherogenic index and HTR tended to decrease, phospholipid level in the serum was shown to increase by A. iwayomogi administration. At the same token, total liver lipids were decreased to 40%. The group fed both high fat and ethanol extract showed 28% decrease of liver lipids compared with the group fed only high fat diet. On the other hand, HDL cholesteol level of high fat and extract fed group was increased to 30% compared with the non-extract-fed control. Liver TG also was decreased to about 21% in the extract fed groups. Liver phospholipids, particularly, exceeded more than double of the level of high fat control. The above results indicate than A. iwayomogi exert a strong hypocholesterolemic and hypolipidemic effect which may lead to the protection from hypertention, obesity, stroke and many other circulatory diseases.

• 자원리싸이클링
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• 제3권3호
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• pp.21-26
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• 1994

국내에서 산출되고 있는 반토혈암으로부터 고순도 $\alpha-Al_2O_3$를 얻기 위하여 황산암모늄을 사용한 염배소와 황산을 이용한 추출 그리고 하소 실험을 하였다. 황산의 농도, 반응시간, 온도 등이 알루미나 추출에 미치는 영향에 대하여 조사하였으며, 반응산물은 X-ray, DTA-TG, 화학분석, SEM 등으로 분석하였다. 추출조건은 전처리 조건으로 황산암모늄의 농도가 0.6M, 배소온도가 $650^{\circ}C$였으며, 황산추출은 황산농도 10%로 240분간 추출할 경우 96%의 수율을 나타내었다. 생성된 $Al_2O_3$ 분말을 X-ray 분석한 결과 $\alpha-Al_2O_3$였으며 그 순도는 99.23%였다.

• 한국체육학회지인문사회과학편
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• 제58권1호
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• pp.271-284
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• 2019

이 연구의 목적은 12주간의 댄스스포츠가 비만노인여성의 신체조성, 아이리신, 아디포넥틴 및 혈중지질에 어떠한 영향을 미치는지 알아보고 비만노인여성을 위한 효과적인 운동프로그램을 제안하는데 있다. 의학적 질환과 증상이 없는 65세 이상 비만노인여성 26명을 대상으로 운동군 13명과 대조군에 13명을 무작위 배정하여 실시하였으며. 운동군은 12주간, 주 2회, 일일 60분 댄스스포츠를 실시하였고 대조군은 동일 처치 기간 동안 일상생활을 유지하도록 했다. 이 연구의 결과에서 혈중 아이리신 농도는 운동군에서 유의하게 증가하였으며, 아디포넥틴 농도는 운동군에서 증가하는 경향을 보였다. 혈중지질의 TG, LDL-C농도는 운동군에서 유의하게 감소하였고, TC과 HDL-C농도는 운동군에 감소 및 증가하는 경향이 나타났다. 이상의 결과를 종합해 보면 낮은 수준의 아이리신과 아디포넥틴, 혈중 콜레스테롤이 댄스 스포츠 운동 후 비만노인여성에 긍정적인 효과가 나타나 노인 비만 관리를 위한 좋은 운동으로 사료된다.

• 대한본초학회지
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• 제39권1호
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• pp.11-21
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• 2024

Objectives : This study aims to analyze the anti-obesity effect of Syzygium aromaticum L. (SA) in obese mice made by a 60% high-fat diet (HFD). Methods : The antioxidant activities of SA were evaluated in vitro. To assess the anti-obesity effect of SA, male C57BL/6 mice were divided into five groups: Normal, Control, GC100 (Garcinia cambogia 100 mg/kg/day), SA100 (SA 100 mg/kg/day), SA200 (SA 200 mg/kg/day). All groups underwent a 6-week regimen of HFD and oral administration, except for the Normal group. Subsequently, we performed blood analysis, western blotting, and histopathological staining. Results : SA demonstrated effectiveness in antioxidant measurements. SA treatment resulted in a significant decrease in body weight gain, along with reductions in liver and epididymal fat weights. Serum triglyceride (TG), total cholesterol (TC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and leptin levels were reduced with SA treatment. Moreover, in the SA100 group, the reduction of both TG and TC synthesis was caused by inhibiting the sterol regulatory element-binding transcription factor 1 (SREBP-1) and sterol regulatory element-binding transcription factor 2 (SREBP-2) through the Sirtuin 1 (Sirt1)/phospho-AMP-activated protein kinase (p-AMPK) pathway. Furthermore, SA treatment at a dose of 100 mg/kg reduced the accumulation of lipid droplets in the liver and the adipocyte size of the epididymal fat. Conclusion : Our research reveals the anti-obesity effects of SA by demonstrating its ability to inhibit body weight gain and lipid accumulation, suggesting that SA might be promising for obesity treatment.