• Physical Therapy Rehabilitation Science
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• 제10권1호
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• pp.48-54
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• 2021

Objective: The purpose of this study is to investigate the effect of exercise programs according to various music tempo on pain, posture, and function of automobile parts manufacturing workers. Design: Two-group pretest-posttest design. Methods: This study was an exercise program tailored to the prevention and treatment of musculoskeletal diseases were performed for a total of 4 weeks and 3 times a week for 60 minutes. The exercise program consisted of customized exercises according to the characteristics of the subject's musculoskeletal system. The subject provided music during the exercise program, and the group who applied the fast tempo music listened to the music of 120~140 bpm, and the group who applied the slow tempo music listened to the music of the low tempo of 60~80 bpm. Results: Significant differences were found in pain and physical function before and after the fast tempo music group and the slow tempo music group (p<0.01). However, there was a significant difference in posture characteristics only in the fast tempo music group (p<0.01). In terms of pain, physical function, and posture characteristics, the fast tempo music group showed significant improvement compared to the slow music tempo group (p<0.05). Conclusions: A customized exercise program using fast-tempo music and slow-tempo music to workers in the automobile parts manufacturing industry, significant differences in pain, physical function, and posture characteristics applied with fast-tempo music were found. In the future, applying an exercise program with a fast tempo is expected to help improve musculoskeletal disorders.

• 한국동물생명공학회지
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• 제37권4호
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• pp.231-238
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• 2022

Reactive oxygen species (ROS) production and F-actin cytoskeleton dynamics play important roles in the survival rate of blastocysts after the vitrified-warming process. However, the protective effects of Mito-TEMPO against cryo-injury and viability through F-actin aggregation and mitochondrial-specific ROS production in vitrificated-warmed bovine embryos have not been investigated. The aims of the present study were to: (1) determine the effects of Mito-TEMPO on embryonic developmental competence and quality by F-actin stabilization during in vitro culturing (IVC), and (2) confirm the effects of Mito-TEMPO through F-actin structure on the cryotolerance of vitrification-warming in Mito-TEMPO exposed in vitro production (IVP) of bovine blastocysts. Bovine zygotes were cultured with 0.1 μM Mito-TEMPO treatment for 2 days of IVC. Mito-TEMPO (0.1 μM) exposed bovine embryos slightly improved in blastocyst developmental rates compared to the non-treated group. Moreover, the viability of vitrified-warmed blastocysts from Mito-TEMPO treated embryos significantly increased (p < 0.05, non-treated group: 66.7 ± 3.2% vs Mito-TEMPO treated group: 79.2 ± 5.9%; re-expanded at 24 hours). Mito-TEMPO exposed embryos strengthened the F-actin structure and arrangement in the blastocyst after vitrification-warming. Furthermore, the addition of Mito-TEMPO into the IVC medium enhanced embryonic survival and quality through F-actin stabilization after the vitrification-warming procedure. Overall, our results suggest that supplementing the culture with 0.1 μM Mito-TEMPO improves the embryonic quality and cryo-survival of IVP bovine blastocysts.

• ETRI Journal
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• 제43권6호
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• pp.1081-1092
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• 2021

Electroencephalography (EEG) recordings taken during the perception of music tempo contain information that estimates the tempo of a music piece. If information about this tempo stimulus in EEG recordings can be extracted and classified, it can be effectively used to construct a music-based brain-computer interface. This study proposes a novel convolutional recurrent attention model (CRAM) to extract and classify features corresponding to tempo stimuli from EEG recordings of listeners who listened with concentration to the tempo of musics. The proposed CRAM is composed of six modules, namely, network inputs, two-dimensional convolutional bidirectional gated recurrent unit-based sample encoder, sample-level intuitive attention, segment encoder, segment-level intuitive attention, and softmax layer, to effectively model spatiotemporal features and improve the classification accuracy of tempo stimuli. To evaluate the proposed method's performance, we conducted experiments on two benchmark datasets. The proposed method achieves promising results, outperforming recent methods.

• 한국식품과학회지
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• 제33권1호
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• pp.40-44
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• 2001

이 연구에서는 TEMPO와 NaBr의 농도 및 온도가 옥수수 전분 내 1차 알코올기의 선택적 산화 반응에 미치는 영향을 조사하였다. TEMPO와 NaBr 및 온도의 수준이 증가함에 따라 반응 시간은 감소되는 경향을 나타내었다. TEMPO, NaBr 및 온도의 수준이 각각 1.7 mM/100 mM AGU, 100 mM/100 mM AGU 및 $14^{\circ}C$까지는 이들의 수준이 증가함에 따라 반응 시간이 급격히 단축되었으나, 이 이후의 수준부터는 단축정도가 적었다. 수율은 NaBr의 수준이 증가함에 따라 감소하였고, TEMPO와 온도는 수율에 큰 영향을 주지 않는 것으로 나타났다. 선택성은 NaBr의 농도와 온도가 각각 100 mM/100 mM AGU와 $6^{\circ}C$까지는 유의적인 차이를 보이지 않았으며, 이 이후의 수준부터 NaBr 및 온도가 증가함에 따라 유의적으로 감소하는 것으로 나타났다.

• 대한감각통합치료학회지
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• 제3권1호
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• pp.57-65
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• 2005

Objective : The purpose of this study was to investigate the effect of sensory stimulation for static balance on normal children. Sensory input was consisted of colors, and music tempo stimulation. Methods : Participants were consisted of 20 normal kindergarten children ranging in age from six to seven years. The static balance was tested by a BPM(Balance Performance Monitor). In this study one-way ANOVA was used and the statistical significance level of results was determined at 0.05. Results : 1. According to color stimulation, there was no significant difference in static balance among red, yellow, green(p>0.05). 2. According to music tempo stimulation, there was no significant difference in static balance, among the high music tempo and low music tempo children in a general environment(p>0.05). 3. However, both green color and low music tempo stimulation have a little effect on static balance on normal children. Conclusion : These results indicate the possibility that the application method of green color and low music tempo stimulation may help in the improvement of static balance for the disabled children. This study will be used as the foundational data of therapeutic environment for the disabled children.

• 펄프종이기술
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• 제47권2호
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• pp.42-52
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• 2015

In this study, cellulose nanofibrils (CNF) were modified through carboxymethylation or TEMPO-mediated oxidation and their effects on ionicity and characteristics of sheet, film, and foam were investigated. Carboxymethylation was carried out on pulp fibers as a pre-treatment before preparation of CNF. The gel-like and translucent CNF hydrogel was obtained by grinding of carboxymethylated cellulose fibers. Carboxymethylated CNF film and freeze dried sheet showed higher transparency than that of untreated CNF. The CNF sheet with high strength and the CNF foam without large ice crystals were obtained by using the carboxymethylated CNF. TEMPO-mediated oxidation was carried out as a post-treatment of CNF. The zeta potential and charge demand of TEMPO-oxidized CNF were increased with an increase in oxidation time and addition amount of NaClO. The density of sheet made of TEMPO oxidized CNF was increased with the amount of oxidizing agent. The TEMPO oxidized cellulose nanofiber (TOCN) which was obtained from supernatant after centrifugation could be converted to transparent film.

• 한국식품위생안전성학회지
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• 제28권3호
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• pp.252-257
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• 2013

표준 평판배지 정량검출법과 TEMPO STA를 이용한 황색포도상구균 정량 분석에 대한 상관성과 유의성을 평가하기 위해 복합조리식품에 황색포도상구균을 인위적으로 접종하여 결과값을 통계 처리하여 유의성을 조사한 결과 두 실험방법간에는 유의적 차이가 없었다(p < 0.05). 상관계수($r^2$)는 0.9672로 두 실험간에는 높은 연관성을 보였다. TEMPO STA를 466개의 시료에 적용한 결과 454개(97.4%)의 시료는 문제가 없었으나 이중 12개의 시료에서는 error가 발생하였다. 그러나 TEMPO법은 실험 단계 간편하고 사용이 쉬우며 신속한 결과를 얻을 수 있으며 자동화된 장비로 실험 과정에 의한 오류를 최소화 할 수 있는 장점이 있으며 표준시험법인 표준 평판 배지법과도 유의적인 차이가 없어 정확한 정량검출이 가능할 것으로 생각된다. 최근 식품의 안전성확보를 위해 검사 품목이 다양해지며 수도 증가하는 추세이기 때문에 황색포도상구균의 정량 검사가 정확하고 신속해야 할 필요가 있으므로 TEMPO STA법의 필요성은 앞으로 크게 늘어날 것으로 보인다.

• Macromolecular Research
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• 제13권3호
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• pp.187-193
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• 2005

The TEMPO-mediated living free-radical bulk and dispersion polymerization of styrene in the presence of camphorsulfonic acid (CSA) are investigated. In the absence of TEMPO and CSA in the bulk polymerization, a conversion of $93\%$ is achieved within 6 hr of polymerization. When only TEMPO is involved in this polymerization, the pseudo-living free-radical polymerization is well achieved, however, the polymerization rate becomes quite slow. This retardation of the polymerization rate is solved by the addition of a low concentration of CSA. In the TEMPO-mediated dispersion polymerization in the presence of CSA, similar trends in the conversion, kinetics, and PDI are observed as those observed in the case of bulk polymerization. When only TEMPO is used in the dispersion polymerization, the resulting particle size becomes quite broad, due to the prolonged polymerization time. However, when a 1.0 molar ratio of CSA to TEMPO is added to the TEMPO-mediated dispersion polymerization, fairly mono-disperse PS microspheres having an average size of 5.83 $\mu$m and a CV of 3.4$\%$ are successfully obtained, due to the narrow molecular weight distribution of the intermediate oligomers and shortening of the polymerization time. This result indicates that the addition of CSA to the TEMPO-mediated bulk and the use of dispersion polymerization not only shortens the polymerization time, but also greatly improves the uniformity of the microspheres.

• 한국동물생명공학회지
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• 제36권4호
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• pp.299-306
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• 2021

Vitrification methods are commonly used for mammalian reproduction through the long-term storage of blastocyst produced in vitro. However, the survival and quality of embryos following vitrification are significantly low compared with blastocyst from in vitro production (IVP). This study evaluates that the survival of frozen-thawed bovine embryos was relevant to mitochondrial superoxide derived mitochondrial activity. Here we present supplementation of the cryopreservation medium with Mito-TEMPO (0.1 µM) induced a significant (p < 0.001; non-treated group: 56.8 ± 8.7%, reexpanded at 24 h vs Mito-TEMPO treated group: 77.5 ± 8.9%, re-expanded at 24 h) improvement in survival rate of cryopreserved-thawed bovine blastocyst. To confirm the quality of vitrified blastocyst after thawing, DNA fragmentation of survived embryos was examined by TUNEL assay. As a result, TUNEL positive cells rates of frozen-thawed embryos were lower in the Mito-TEMPO treated group (4.2 ± 1.4%) than the non-treated group (7.1 ± 3.5%). In addition, we investigated the intracellular ROS and mitochondrial specific superoxide production using DCF-DA and Mito-SOX staining in survived bovine embryos following vitrification depending on Mito-TEMPO treatment. As expected, intracellular ROS levels and superoxide production of vitrified blastocysts after cryopreservation were significantly reduced (p < 0.05) according to Mito-TEMPO supplement in freezing medium. Also, mitochondrial activity measured by MitoTracker Orange staining increased in the frozen-thawed embryos with Mito-TEMPO compared with non-treated group. These results indicate that the treatment of Mito-TEMPO during cryopreservation might induce reduction in DNA fragmentation and apoptosis-related ROS production, consequently increasing mitochondrial activation for developmental capacity of frozen-thawed embryos.

• 한국식품위생안전성학회지
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• 제32권4호
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• pp.249-253
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• 2017

본 연구는 자동화 MPN 분석 장비인 TEMPO (TEMPO BC)와 MYP 배지법을 이용한 바실러스 정량 시험 결과에 대한 상관관계와 유의성을 연구하였다. 자연적으로 오염되어 있는 B. cereus 다빈도 검출 식품을 선별하여(N = 384) 두 시험법으로 정량 시험한 결과값의 차가 1 log CFU/g 미만이 95.3%였으며, B. cereus가 검출된 시료에서는 92.5%였다. 통계학적으로 TEMPO BC 시험법과 MYP 배지법은 유의적 차이가 없었다(p < 0.05). 국내 정량 규격이 있는 식품에 인위적으로 B. cereus를 접종하고 두 시험법을 비교하였다. $R^2$값은 소스류(0.98), 조림식품류(0.98), 젓갈류(0.96)에서 유의적인 차이가 없었으나, 생식류(0.94), 청국장류(0.86), 된장류(0.71)는 시험법 간의 유의적인 차이가 있었다. 콩, 메밀 등의 곡류 제품은 TEMPO BC 결과값의 오류가 날 수 있는 재료로 명시되어 있으므로 선식류와 청국장류, 된장류는 자동화 시험법인 TEMPO BC로 사용하기 위해서는 시험법을 추가적으로 검증 할 필요성이 있다고 생각된다. 자연적으로 오염되어 있는 식품 시료와 인위적으로 접종한 시료에 대한 선형 방정식은 $log(TEMPO\;BC)=0.8453{\times}log$ (MYP배지) + 0.1642이며 paired t-test를 이용하여 검증한 결과 유의적인 차이가 없었다. 최근 식품의 안전성에 대한 소비자의 관심이 증가하여 식품공전 및 국제 기준에 정량기준이 강화되고 있는 추세이므로 TEMPO BC의 수요증가가 예상된다. 따라서 본 연구는 이에 대비하여 식품의 안전성 평가에 사용되는 기존의 실험법과의 비교 및 타당성 연구 기초 자료로 쓰일 것이라 생각된다.