• Applied Microscopy
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• 제51권
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• pp.19.1-19.7
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• 2021

The main purpose of this paper is the preparation of transmission electron microscopy (TEM) samples from the microsized powders of lithium-ion secondary batteries. To avoid artefacts during TEM sample preparation, the use of ion slicer milling for thinning and maintaining the intrinsic structure is described. Argon-ion milling techniques have been widely examined to make optimal specimens, thereby making TEM analysis more reliable. In the past few years, the correction of spherical aberration (Cs) in scanning transmission electron microscopy (STEM) has been developing rapidly, which results in direct observation at an atomic level resolution not only at a high acceleration voltage but also at a deaccelerated voltage. In particular, low-kV application has markedly increased, which requires a sufficiently transparent specimen without structural distortion during the sample preparation process. In this study, sample preparation for high-resolution STEM observation is accomplished, and investigations on the crystal integrity are carried out by Cs-corrected STEM.

• 한국동물위생학회지
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• 제34권1호
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• pp.37-43
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• 2011

This study was conducted to investigate the prevalence and genotypes of extended-spectrum ${\beta}$-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3~4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both $bla_{CTX-15}$ and $bla_{TEM-1}$, and one isolate from chicken have $bla_{CTX-15}$ and $bla_{TEM-116}$. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that $bla_{TEM-116}$ gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.

• Applied Microscopy
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• 제36권1호
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• pp.1-6
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• 2006

고분해능 TEM 영상 이론의 결맞음 조산에서의 결맞음에 관한 개념을 소개하였다. 현미경에서 부분 결맞음 파동으로 인한 transfer function과 envelope의 발생을 설명하고 현미경의 분해능과 관련된 passband와 Scherzer 초점 조건을 소개하고 결맞음 특성에 관하여 소개하였다.

• 한국전자파학회논문지
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• 제16권8호
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• pp.810-814
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• 2005

TEM 셀 내에 있는 혈액에 대한 삽입 손실과 반사율을 측정하여, 원하는 혈액 흡수 SAR(Specific Absorption Rate) 선택에 따른 TEM셀 입력 전력을 구하였다. 혈액 세포는 피부 세포와 함께 전자파 영향을 연구하기 위한 기준 세포이다. 따라서 SAR에 따른 혈액 세포에 인가할 전력 산출은 정확해야 함은 물론 타당성이 있어야 한다. 이 연구에서는 g당 혈액 세포가 받는 전력을 산출하기 위해 50 g의 혈액을 이용하여 전달 전력 감소를 측정하였다. 측정된 데이터로부터 휴대폰 전자파 생체 흡수율 SAR 0.8, 1.6, 3, 4 mW/g에 해당하는 TEM셀 입력 전력을 계산하였다. 이 연구 결과는 배양 세포의 전자파 방사 장치에 응용함을 목적으로 하고 있다.

• Applied Microscopy
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• 제31권4호
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• pp.315-323
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• 2001

Kaolinite분말 시료를 이용한 TEM 직접 가열 실험에서 얻은 결론은 아래와 같다. (1) Gatan사의 가열 시료지지대에서 사용하고 있는 증류수 순환장치는 $500^{\circ}C$이상의 가열 실험에서 시료의 유동을 막는 필수적인 장비이다. (2) $600^{\circ}C$이하의 가열 실험에서는 분말의 두께에 따른 상전이 진행정도의 차이가 나타나기 때문에 앙은 시편과 두꺼운 시편을 고루 관찰하는 노력이 필요하다. (3) $900^{\circ}C$ 이상의 고온 가열 실험에서는 융점이 높은 Mo-grid의 사용이 필수적이다. 전체적으로 온도 상승률이나 온도 지속 시간이 상전이 과정에 미치는 영향이 증가하기 때문에 이들의 정량적인 제어가 가능한 자동제어방식의 사용이 필요하다. (4) 단면 관찰을 위해 고온용 epoxy에 포매된 kaolinite분말의 TEM가열 실험은 고온 가열시 epoxy의 유동 문제를 극복할 새로운 방법의 개발이 필요하다.

• 한국세라믹학회:학술대회논문집
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• 한국세라믹학회 2000년도 추계총회,초청강연,특별강연,연구발표회
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• pp.186.1-186
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• 2000

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집E
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• pp.100-106
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• 2001

The size distributions of electrospray droplets from the Taylor cone in cone-jet mode are directly measured by using a freezing method and a transmission electron microscope (TEM) image processing technique. These results are compared with the data obtained by an aerodynamic size spectrometer (TSI Aerosizer DSP). The use of glycerol seeded with NaI and a freezing method make it possible to sample droplets with their original sizes preserved. Since pictures of droplets are taken with TEM with very low vapor pressure of the solution, evaporation is suppressed by freezing. For liquid flow rates below 1 nl/sec, the measured droplet diameters by the TEM image processing technique and the aerosizer are in the range of 0.25 to $0.32{\mu}m$ and 0.30 to $0.40{\mu}m$, respectively. Comparing the TEM data with the aerosizer measurements, it has been revealed that the TEM image processing technique can afford more accurate values of droplet size distributions in the submicron range of 0.1 to $0.4{\mu}m$.

• 전기학회논문지
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• 제63권3호
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• pp.404-408
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• 2014

In this research we evaluate the excitation homogeneity and SAR of three different Tx arrays after B1+ Shimming in the human body at 3T. Through the simulations, we compared the field properties that are standard deviation and mean value of transverse magnetization for 1) strip line TEM array, 2) rectangular loop array, 3) combined array of strip line element and rectangular loop and shown the utilities of B1+ shimming in human body model. After B1+ shimming, it is evaluated four different types of SAR for body mesh through whole body simulation; those are average, maximum 1-cell, maximum 1-gram, and maximum 10-gram SAR. It appears that in this particular comparison an array based on strip line elements can produce better homogeneity and lower SAR than an array of rectangular loops or an array of combined elements (strip line and loop). While many factors are considered in designing coils for production, it is hoped that methods and results like these will be used in the future to guide decisions and maximize benefit.

• 한국결정성장학회지
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• 제26권1호
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• pp.1-7
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• 2016

RE : YAG 단결정은 레이저 발진 소재로 다양한 성장 변수를 제어하면서 Czochralski법으로 성장된다. 성장과정 동안 고액계면의 온도구배 및 회전속도에 의해 발생하는 결함들은 결정의 광학적 특성 저하로 작용하기 때문에 결함 분석을 통한 결정 품질의 향상을 필요로 한다. 격자결함 밀도 분석(EPD)을 통하여 성장된 RE : YAG 단결정의 표면 결함 존재를 확인하였고, 이를 통해 투과전자현미경(TEM) 분석영역을 선택하였다. 선택한 영역의 시편은 트라이포드 연마 방법으로 제작하였고, 200 kV 투과전자현미경과 300 kV 전계 방사형 투과전자현미경(FE-TEM)을 사용하여 buckling, rod shaped, 내부응력에 의한 bend contours, 편석 등의 결함들을 관찰하였다.

• 한국초전도ㆍ저온공학회논문지
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• 제8권1호
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• pp.9-14
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• 2006

Sample preparation plays a critical role in microstructure analysis using TEM. Although TEM specimen has been usually prepared by jet-polishing or Ar-ion beam milling technique. these methods could not be applied to YBCO CC which is composed of IBAD or RABiTS substrates, several buffet layers, and YBCO superconducting layer because of big difference in mechanical strengths between the metallic phase and oxide phases. To obtain useful cross-sectional information such as interface between the phases or second phases in YBCO CC, it is prerequisite to secure the large area of thin section in the cross-sectional direction. The superconducting layer or the buffer layers are relatively weak and fragile compared to the metallic substrate such as Ni-5wt%W RABiTS of Hastelloy-based IBAD, and preferential removal of weak ceramic phases during polishing steps makes specimen preparation almost impossible. Tripod polisher and small jig were home-made and employed to sample preparation. The polishing angle was maintained <$1^{\circ}$ throughout the polishing steps using 2 micrometers attached to the tripod plate. TEM specimens with large and thin area could be secured and used for RABiTS/IBAD substrate analyses. In some cases, additional Ar-beam ion milling with low beam current and impinging angle was used for less than 30 sec. to remove debris or polishing media attacked to the specimens.