• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.117-117
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• 2003

체외에서 한우 난포란의 감수분열과 배발달 능력의 획득에는 단백질 합성이 수반되어야 한다. 그러나 이러한 변화와 관련된 연구 보고는 거의 전무한 실정이다. 본 실험은 난자의 핵성숙과 관련된 세포질내 단백질 변화를 파악하기 위하여, 체외성숙 시간에 다른 배발달율과 세포질내 단백질을 비교하여 배발달능력 획득과 관련 있는 단백질을 규명하고자 실시하였다. 한우 난소에서 2-8mm의 가시난포로부터 난포란을 회수하였다. 회수된 난포란은 10% FBS와 호르몬이 첨가된 TCM199 용액에서 18시간 또는 24시간 체외성숙을 실시하였다. 난자 세포질내 단백질 변화는 2D gel electrophoresis를 이용하였고, 유의적인 변화를 나타낸 spot은 peptide mass fingerprinting을 통하여 단백질 동정을 실시하였다. 체외수정은 fer-TALP 용액을, 체외배양은 CR1aa 용액을 배양 3일째까지는 0.3% BSA, 그 이후에는 10% FBS와 난관상피세포를 첨가하여 사용하였다. 통계분석은 t-test를 이용하였다. 난자의 세포질에 대한 이차원전기영동 결과 29개의 단백질 spot들을 확인하였다. 한편 체외성숙 18시간째에 PB가 출현된 난자는 PB가 출현되지 않은 난자에 비하여 15개의 spot에서 유의적인 변화를 나타냈다. 이들 중 4개의 단백질 spot은 낮았고, 11개 spot의 수준은 높은 경향이었다. 체외성숙 18시간째와 24시간째의 배발달율을 조사한 결과 18시간째에서 유의적으로 높은 배반포 발달율을 나타냈다. 그리고 체외성숙 18시간과 24시간째 난자의 세포질내 단백질 spot들의 변화를 비교한 결과 PB가 출현된 난자 세포질에서 단백질의 변화와 유사한 경향이었다. 그러나 2개의 단백질 spot은 상반된 경향을 나타냈다. 따라서 본 실험에서 난자의 핵성숙과 관련 있는 15개의 spot을 확인하였고, 이들 단백질 spot중에서 2개가 배발달 능력과 관련이 있을 것으로 사료된다.보다는 육질등급에 많은 영향을 받는 것으로 판단된다. 한편 육질 1등급에서 배발달율이 낮은 이유는 육질 향상을 목적으로 암소를 비육 하는 경우 발생하는 번식장애와 밀접한 관계가 있는 것으로 사료된다.각각 가장 높았다. 배양 8일째 배반포의 세포수에 있어서 총세포수와 TE 세포수는 차이가 없었으나, ICM 세포수가 l0mg 첨가군에서 가장 높았다. 본 실험 결과에서 체외성숙 배지에 NEAA와 EAA 첨가가 배발달율에는 효과가 없었지만, 첨가농도의 증가에 따라 ICM 세포수가 증가하였다. 한편 체외성숙 배지에 LAH 첨가는 첨가 농도가 높을수록 배발달율은 낮았지만 ICM 세포수는 증가하였다.에 Csk가 관여하고 있음을 알 수 있다. 결론적으로 성적 성숙에 따른 생쥐 정소 내 Src-Csk loop의 발현과 Src kinase 활성의 변동은 정소 내 간충조직, 세정관 상피의 증식 및 기능적 분화 과정을 매개하는 생리적 활성분자 수용체 하위의 신호전달 과정에 Src-Csk loop에 의한 조절가능성을 확인할 수 있었다.rugrene의 향기성분이 주요 성분군으로 확인되었다. 2. 생강나무에서 생강의 향기를 발산하는 성분으로는 $\beta$-myrcene, o-terpinolene, phellandrone, ι-limonene, $\beta$-eudesmol, $\delta$-cadinone, elemol, trans-caryophyllene으로 동정되었으며 그 중에서도 phellandrene, $\beta$-eudesmol이 주된 역할을 하는 성분으로 확인하였다. 유의적인 관련성이 나타났고, 복부 비만의 지표인 허리엉덩이둘레비는 GPT, alkaline phosphatase, 공복시 혈당 및 MCV 등 다양한 건강지표와 관련성을 나타내어 향후 비만에 있어 다양한 혈액 성상의 변화 및 역할규명에 대한 연구가 이루어져야 할 것으로 본다.hat

• Journal of Acupuncture Research
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• v.29 no.4
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• pp.105-111
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• 2012

Objectives : An objective measurement for the effects of acupuncture therapy had been regarded as one of the important agendas in the research of acupuncture. This case report aimed to measure the changes of blood flow on the skin flap after one session of electroacupuncture treatment. Methods : A 27-year-old male patient was treated with ipsilateral or contralateral electroacupuncture on the $LI_{11}$, $LU_{10}$, $LI_{10}$, $TE_5$, $SI_3$ and Ex- $UE_9$(Palsa) in the arm with the skin flap on dorsal region of the hand. Changes of blood perfusion on the skin flap and opposite dorsum of hand were measured with laser Doppler blood perfusion imager(LDPI). Results : Immediate post-treatment relative changes of the blood perfusion on the skin flap and opposite hand after one session of ipsilateral electroacupuncture was 21% and 18% higher than values from pre-treatment assessment, respectively. On the contrary, when needled contralaterally, relative changes of the blood perfusion on the affected and unaffected hand was 7% lower and 22% higher than values from pre-treatment assessment, respectively. Mean differences of the blood perfusion between on the skin flap and on control hand remained similar after the ipsilateral acupuncture treatment but substantially increased(44.58 of perfusion units). Conclusions : Electroacupuncture may alter the local blood flow on the skin flap. More study is needed to investigate the effects of acupuncture on the blood flow and microcirculation on the skin tissue by well-designed clinical and experimental studies.

• Physical Therapy Korea
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• v.10 no.3
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• pp.17-27
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• 2003

Repetitive transcranial magnetic stimulation (rTMS) modulates cortical excitability beyond the duration of the rTMS trains themselves. Depending on rTMS parameters, a lasting inhibition or facilitation of cortical excitability can be induced. Therefore, rTMS of high or low frequency over motor cortex may change certain aspects of motor learning performance and cortical activation. This study investigated the effect of high and low frequency subthreshold rTMS applied to the motor cortex on motor learning of sequential finger movements and brain activation using functional MRI (fMRI). Three healthy right-handed subjects (mean age 23.3) were enrolled. All subjects were trained with sequences of seven-digit rapid sequential finger movements, 30 minutes per day for 5 consecutive days using their left hand. 10 Hz (high frequency) and 1 Hz (low frequency) trains of rTMS with 80% of resting motor threshold and sham stimulation were applied for each subject during the period of motor learning. rTMS was delivered on the scalp over the right primary motor cortex using a figure-eight shaped coil and a Rapid(R) stimulator with two Booster Modules (Magstim Co. Ltd, UK). Functional MRI (fMRI) was performed on a 3T ISOL Forte scanner before and after training in all subjects (35 slices per one brain volume TR/TE = 3000/30 ms, Flip angle $60^{\circ}$, FOV 220 mm, $64{\times}64$ matrix, slice thickness 4 mm). Response time (RT) and target scores (TS) of sequential finger movements were monitored during the training period and fMRl scanning. All subjects showed decreased RT and increased TS which reflecting learning effects over the training session. The subject who received high frequency rTMS showed better performance in TS and RT than those of the subjects with low frequency or sham stimulation of rTMS. In fMRI, the subject who received high frequency rTMS showed increased activation of primary motor cortex, premotor, and medial cerebellar areas after the motor sequence learning after the training, but the subject with low frequency rTMS showed decreased activation in above areas. High frequency subthreshold rTMS on the motor cortex may facilitate the excitability of motor cortex and improve the performance of motor sequence learning in normal subject.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.8 no.1
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• pp.14-22
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• 1972

For the development of acoustic fishing method, the noises of fishes have been recorded and analy/'ed by many scientists. Some specimens of fishes were selected as such Cyprinus carpio, Ctenopharyngodon idellus Carassius carassius, and pagrosol1ms major in this experiment. The noises such as feeding noise, driving away noise, jumping noise and fi llip noise were recorded by the tape recorder, Sony Model 262, through the underwa te r microph I one, Oki ST 6582, and analyzed in frequencies bv octave band analyzer, Rion SA-55, and sound pressure level of source by sound level meter, Rion NA-opNN The supplied feed was placed within 5em apart from the hydrophone. The result of analyzed noises were as follow. Cyprinus carjJio; Feeding noise 250- 500 cps, 92- 99 dB Driving away noise 125-2, 000 eps, 101-112 dB Jumping noise 125-2, 000 eps, 99-116.5 dB Ctenopharyngodon idcllus; Driving away noise 125-1, 000 cps, 96-109 dB Carassius carassius; Feeding noise 250- 500 cps, 91. 5- 99.5 dB Driving away noise 125-1, 000 eps, 99-108 dB Carassius auratus Feeding noise 250 eps, 94-101 dB Driving away noise 125-1, 000 cps, 98-110 dB Pagrosomus major Feeding noise 230-500 cps, 90-101 dB Fillip noise 500 cps, 98-108 dB (1) Feeding noise was produced as like as snap noise of twig and gulping down saliva noise in human and dominant frequency range of the noise is 250-500 cps and noise level 90-101 dB. (2) It was found that feeding noise were not a monotonic but a complex tones though fish took the same food. (3) Driving away noise was produced not so keen and the wave form of the noise is rising very sharp and big amplitude in the oscillograph. Dominant frequency range of this noise was about 150-1, 000 cps and noise level 96-112 dB except thut of carp. (4) The frequency of snapper's fillip noise, when it produced by caudal fin in swimming at the surface of water, was 500 cps and noise level 93-108 dB snd that of jumping noise of carp about 150-2, 000 cps and noise level 99-116.5 dB.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1666-1685
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• 2004

This present study was designed to screen medicinal plants for the treatment of brain diseases such as Parkinson's disease or aging. We tested the effects of the water extracts from 38 species medicinal plants on antioxidant capacity, monoamine oxidase B (MAO-B) inhibitory activity, acetylcholinesterase (AChE) inhibition and antiperoxidation activity in vitro. The water extracts from 38 species were tested on their antioxidant activity using radical scavenging effects against ABTS+. The water extract of C. sappan was showed the highest antioxidant capacity, the antioxidant activity at 1 Jig of herbal extract being 0.38mM TE. Lipid peroxidation in brain homogenates induced by NADPH and ADP-Fe/sup 2+/ was strong inhibited by C. sappan and R. palmatum extracts. Among the 38 medicinal plants investigated, R. palmatum showed significant biological activity (antioxidant capacity, MAO-B inhibiory activity, and AChE inhibitory activity). The protective efficacy of R. palmatum water extract on 1-methyl-4­phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism and its possible mechanism were studied in C57BL/6 mice. Treatment of R. palmatum water extract protected biomacromolecules such as lipids from oxidative damage induced by MPTP. The content of MDA in brain tissue was decreased significantly by R. palmatum extract. These results suggest that R. palmatum water extract plays on effective role in attenuating MPTP-induced neurotoxicity in mice. This protective effect of R. palmatum might be estimated the result from the inhibitory activity on monoamine oxidase B and the enhancement of antioxidant activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.1
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• pp.77-81
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• 1985

It was investigated about extractability and biological property(ATPase activity) of actomyosin from skeletal muscle of chi(:ken differed feeding period. The extractabilities of actomyosin from pectoral muscle were increased from 184.5 to 1020.1 mg per 100g muscle as feeding period prolonged from 3 weeks to 8 weeks. In case of leg-muscles, extractability was revealed the similar tendency as pectoral muscles. EDTA ATPase activity of actomyosin in various chicken muscles for 3 weeks feeding was 0.6 Brmole Piimg Protein/min., 0.59 for 6 Iveeks feeding and 0.50 for 8 weeks. The Ma^{+2}$-ATPase of actomyosin in various chicken muscles was showed inverted relationship with ionic strength. EGTA ($125\;{\mu}mole$)inhibited Ma^{+2}$-ATPase activity to below $0.1\;{\mu}mole$ Pi/mg protein/min. regardless the feeding period.

• 한국유가공학회:학술대회논문집
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• 1997.05a
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• pp.41-61
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• 1997

젖산균의 유전자 연구를 촉진하기 위해 간단하고 신속한 plasmid DNA의 분리방법과 electro-poration을 이용하여 vector plasmid의 간단하고 신속한 전이방법을 얻기 위해 젖산균의 형질전환에 영향하는 요인에 대하여 연구하였으며 연구결과는 다음과 같다. 1. O'Sullivan과 Klaenhammer의 방법을 개선하여 젖산균 plasmid DNA의 분리에 좋은 결과를 얻을 수 있는 신속하고 쉬운 방법을 고안하였으며, genomic DNA 분리에 이용되는 guanidium thio-cyanate 처리방법을 plasmid의 분리에 적용할 수 있었다. 2. L. casei, L. acidophilus. L. delbruekii var. bulgaricus. L. brevis와 L. plantarum 균주에서 plasmid를 확인하였으며, 돼지 분에서 분리된 L. lactis ssp. lactis. L. fermentum과 L. plantarum에서도 plasmid를 분리 확인하였다. 3. Lactococci의 plasmid분리는 lactobacilli와는 달리 mutanolysin의 처리없이도 잘 되었으며, L. lactis ssp. lactis와 Ent. faecalis에서 plasmid를 확인하였다. 4. E. coli plasimd 분리에 이용되는 MPS membrane filter 방법으로 젖산균 plasmid pLZ12의 분리가 가능하였으나, 세포파편이 filter를 막아 사용에 어려움이 있는 것으로 확인되었다. 5. Plasmid 분리없이 electroporation을 이용한 세포 대 세포 전이법으로 간편하고 빠르게 E. coli DH5${\alpha}$에 E. coli Jm109의 plasmid pBX19, pBR322를 전이시켰다. 6. L. lactis ssp. lactis 균주에 lysozyme 처리시 30${\sim}$80%의 생존율을 보였으며, 대부분의 L. acidophilus 균주의 경우 약 70%의 생존율을 보였다. L. casei 102S의 경우는 45분간 처리 시에도 100%의 생존율을 보였다. 8. L. lactis ssp. lactis 균주에 pLZ12를 6.0kV에서 전이시킨 결과 12.5kV에서보다 형질전환 효율이 훨씬 높았으며 lysozyme 처리에 의해 형질전환 효율이 증가되었다. 9. L. acidophilus 균주에 pLZ12를 전이시 6.0kV에서는 전이가 모두 이루어졌으나, 12.5kV에서는 L. acidophilus WIESBY와 NCFM에서 전이가 이루어지지 않았으며, lysozyme 처리 후 pLZ12를 전이시켰을 때 12kV보다 6.0kV에서 형질전환 효율이 증가되었다. 10. Gene Pulser와 Progenitor II를 사용하여 pLZ12를 L. lactis ssp. lactis 균주에 전이하였을 때 Gene Pulser에 비해 Progenitor II의 형질전환 효율이 현저히 떨어졌다. L. acidophilus HY7008과 HY7001은 두 기기 모두 형질전환이 이루어졌으나, L. acidophilus WEISBY와 NCFM은 Progeni-tor II에서 전이가 일어나지 않았으며, Gene Pulser에서 전이균주를 얻어 두 electroporator간에 형질전환 효율의 차이를 보였다. 11. L. casei 102S에 pLZ12를 electroporation시 낮은 전압에서 형질전환 효율이 비교적 좋았으며, 배양 시기를 달리하여 전이시켰을 때 대수생장 말기의 세포가 형질전환 효율이 좋았다. 12. L. casei 102S세포를 각각 10% glycerol, EB, 2차 증류수 등에 녹여 electroporation을 실시하였을 때 각각 $3.8{\times}10^3$, $5.0{\times}10^2$,1.5${\times}10^2$cfu의 형질전환 효율을 보였으며, 1.0mM HEPES, TE buffer를 사용하였을 때에는 전이가 이루어지지 않았다. 13. Plasmid pLZ12의 농도를 달리하여 electroporation을 하였을 때 형질전환 효율이 농도에 비례하여 증가하였다. 14. L. casei 102S에 대수생장 말기의 세포를 채취하여 10% glycerol, 200 Ohms, 25 ${\mu}$FD, 10kV/cm로 plasmid pLZ12를 electroporation할 때 최대 형질전환 효율인 3.8${\times}$10$^{3}$cfu를 얻었으며, lysozyme 처리가 다른 젖산균과는 달리 형질전환 효율을 증가시키지 못하였다. 15. L. casei 102S 세포를 10% glycerol과 EB에 녹여 -20$^{\circ}C$에서 냉동시킨 다음 1일과 7일 후의 세포를 electroporation한 결과 냉동시 세포에 손상을 주는 것으로 인식되었다.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.4
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• pp.237-242
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• 2000

This study was conducted to investigate the effects of cutting frequency on dry matter yield and nutritive value between heading and headless varieties of sorghumxsudangrass hybrid in 1998. Treatments were var. TE hay grazer(heading type) and Jumbo(headless type) as main plot, and 1, 2, and 3 cutting times per a year as sub plot. Although plant height of sorghumxsudangrass hybrid was no significant difference among treatments, headless type was very slightly higher than that of heading type, and both of variety decreased with 1, 2, and 3 cutting times in order. Also, dry matter yield of sorghum~sudangrass hybrid was no significant difference between heading and headless type. Dry matter yield of first cutting plot in heading type and in headless type was the higher than those of second and third cutting plot. Acid detergent fiber and neutral detergent fiber of headless type were slightly higher than that of heading type. Acid detergent fiber of sorghumxsudangrass hybrid was highest at first cutting plot in both varieties. Neutral detergent fiber of second cutting plot in heading type and first cutting plot in headless type were the highest respectively. In vitro dry matter digestibility of headless type was very slightly higher than that of heading type. And in vitro dry matter digestibility of second cutting plot was the highest in both varieties. In vitro digestible dry matter of sorghumxsudangrass hybrid was no significant difference among treatments. The results demonstrated that although there was not significant difference, cultivation of headless type and first cutting plot per a year were very good for nutritive value and DM yield of sorghumxsudangrass hybrid as summer forage crops in Korea. (Key words : Heading, Headless type, Cutting frequency, Sorghum X Sudangrass Hybrid)

• The Journal of Korean Society for Radiation Therapy
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• v.19 no.1
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• pp.7-17
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• 2007

Purpose: As increasing complexity of modern radiotherapy technique, more developing dosimetry is required. Polymer gel dosimeters offer a wide range of potential applications with high resolution and assured quality in the thee-dimensional verification of complex dose distribution such as intensity-modulated radiotherapy (IMRT). The purpose of this study is to find the most sensitive and suitable gel as a dosimeter by varying its composition ratio and its condition such as temperature during manufacturing. Materials and Methods: Each polymer gel with various ratio of composition was irradiated with the same amount of photon beam accordingly. Various polymer gels were analyzed and compared using a dedicated software written in visual C++ which converts TE images to R2 map images. Their sensitivities to the photon beam depending on their composition ratio were investigated. Results: There is no dependence on beam energy nor dose rate, and calibration curve is linear. Conclusion: The polymer gel dosimeter developed by using anti-oxidant in this study proved to be suitable for dosimetry.

• The Transactions of the Korea Information Processing Society
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• v.4 no.6
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• pp.1418-1432
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• 1997

Most general content_based image retrieval techniques use color and texture as retrieval indices. In color techniques, color histogram and color pair based color retrieval techniques suffer from a lack of spatial information and text. And This paper describes the design and implementation of content_based image retrieval system using color space and keywords. The preprocessor for image retrieval has used the coordinate system of the existing HSI(Hue, Saturation, Intensity) and preformed to split One image into chromatic region and achromatic region respectively, It is necessary to normalize the size of image for 200*N or N*200 and to convert true colors into 256 color. Two color histograms for background and object are used in order to decide on color selection in the color space. Spatial information is obtained using a maximum entropy discretization. It is possible to choose the class, color, shape, location and size of image by using keyword. An input color is limited by 15 kinds keyword of chromatic and achromatic colors of the Korea Industrial Standards. Image retrieval method is used as the key of retrieval properties in the similarity. The weight values of color space ${\alpha}(%)and\;keyword\;{\beta}(%)$ can be chosen by the user in inputting the query words, controlling the values according to the properties of image_contents. The result of retrieval in the test using extracted feature such as color space and keyword to the query image are lower that those of weight value. In the case of weight value, the average of te measuring parameters shows approximate Precision(0.858), Recall(0.936), RT(1), MT(0). The above results have proved higher retrieval effects than the content_based image retrieval by using color space of keywords.