• 한국자원식물학회지
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• 제17권2호
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• pp.161-168
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• 2004

Ferritin light heavy chain (FLHC) gene는 일부 중금속과 결합, 저장 및 운반하여 무독화 시킬 수 있는 것으로 알려져 있다. Fe 관련 유전자인 FLHC유전자를 식물 발현용 promoter인 35S promoter와 Tnos를 사용하여 식물 형질전환용 vector를 재조합하였다. 식물세포형질전환용 binary vector는 상기 cassette vector가 조립이 매우 양호하며 border sequence를 가지고 있는 pRD400 binary vector를 사용하여 최종적으로 가나마이신 내성 유전자 (NPT II gene)와 tadpole ferritin heavy chain gene 및 human ferritin light chain gene를 함유하고 있는 binary vector를 재조합하였다. Binary vector의 아그로박테리움에 도입은 triparental mating 방법에 의하여 수행하여 AB배지 및 가나마이신 함유 배지에서 disarmed Ti-vector를 가지고 있는 Agrobacterium tumefaciens MP90/FLHC을 선발하였다. FLHC 유전자 도입된 식물형질전환용 binary vector를 이용하여 형질전환방법을 변형하여 많은 embryo를 유도하였으며 유도된 embryo들은 GA 10mg/L가 첨가된 배지에 지상부를 유도하였다. 형질전환체식물체의 정상적인 생장을 유도하기 위해 최적의 배양조건을 조사하였던 바, 비교적 1/3 MS배지에서 뿌리의 생장과 지상부의 생장이 균일하게 생장하는 경향을 보였으며, 뿌리와 줄기가 잘 발달된 약 7cm의 유식물체를 대량으로 증식하여, 모래와 흙이 1:1로 혼합된 토양에 옮겼다.

• 생태와환경
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• 제42권1호
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• pp.32-38
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• 2009

긴꼬리투구새우 알의 온도별 부화율을 파악하기 위하여 여러 온도조건 (10, 15, 20, 25, 30, 35, 40, 45, $50^{\circ}C$)으로 설정된 인큐베이터에서 실험을 수행하였다. 실험재료로 사용된 긴꼬리투구새우의 알은 2006년 7월 경상북도 안동시 일대의 농경지에서 포획한 것이다. 부화 성공률은 $35^{\circ}C$에서 80.0 (${\pm}9.43$)%로 가장 높게 나타났다. 온도조건 중 $10^{\circ}C$와 $50^{\circ}C$에서는 부화 현상이 일어나지 않았다. 본 연구 결과 긴꼬리투구새우의 부화 최저임계온도는 $6.66^{\circ}C$, 최적 부화 온도는 $35^{\circ}C$로 나타났으며, 부화 가능 온도 범위는 $15{\sim}45^{\circ}C$로 비교적 넓은 것으로 확인되었다.

• 대한치과의사협회지
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• 제11권8호
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• pp.525-530
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• 1973

True collagenolytic enzymes in animal tissues were first demonstrated by Gross and Lapiere (1962), who showed the ability of such an enzyme in the culture medium of living explants of tadpole tissue to degrade a specific substrate of undenatured collagen under physiological conditions. Recently, tumor-associated collagenolytic activity has been demonstrated in human neoplasm and in ascites V Carcinoma. This investigation have been peforme to determine whether or not a collagen lytic enzyme could e found in isolated solid Tawa sarcoma of Donryu female rat obtained the culture medium. The results were as follows. 1. 11.5mg% of hydroxyproline contained in Donryu rat skin collagen, which was extracted by 0.5M acetic acid. 2. Cultivation of solid Tawa sarcoma tissues on reconstituted rat skin collagen gels showed lysis of adjacent gel after 18 hours, and much more extensive lysis after 5 days. 3. Collagen substrate was not attacked by the common proteolytic enzymes, trypsin, pepsin, and pronase.

• 한국조명전기설비학회지:조명전기설비
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• 제9권1호
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• pp.30-36
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• 1995

지금까지 TV와 모니터에서 발생되는 유해한 전자파와 정전기에 대해서는 단순한 차폐방법으로만 대응해 왔다. 본 연구에서는 이와는 달리 생체에 유익한 특성을 역으로 제공하는 방법에 착상하여 생체에 유익한 것으로 알려진 원적외선이 방사되도록 특수세라믹스를 브라운관에서 적용하고 이를 이용하여 생체실험을 한 결과, 일반 TV와 모니터에서보다 국화는 약 1.5배, 초파리와 올챙이는 약 2배 향상된 생명력이 나타났다. 결과적으로 원적외선이 방사되는 특수처리한 브라운관이 생체유익효과를 제공한다는 사실이 입증되었다.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.37-42
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• 2002

In ascidians, a primitive chordate, maternal cytoplasmic factors and inductive interactions are involved in the specification of cell fate in early embryos. The larval structure of ascidians is relatively simple, and the major mesodermal tissues of the tadpole larva are notochord, muscle and mesemchyme. Formation of muscle cells is a cell-autonomous process, and localized maternal macho-1 mRNA specify muscle fate in the posterior marginal zone of the early embryo. In contrast, inductive influence from endoderm precursors plays important roles in the specification of notochord and mesenchyme fates. FGF-Ras-MAPK signaling is involved in the induction of both tissues. The difference in responsiveness of the posterior mesenchyme and anterior notochord precursors is caused by the presence or absence of the posterior-vegetal egg cytoplasm, respectively. In these cases, directed signal may polarizes the responding cells and cause asymmetric cell divisions that operate in both the anterior and posterior regions.

• Parasites, Hosts and Diseases
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• 제35권2호
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• pp.75-78
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• 1997

In uitro excystation studies were done on the metacercarial cysts of Echinostomn triuolvis obtained from the kidneys of naturally infected Rnna species tadpoles. Cysts were excysted in an alkaline trypsin-bile salts medium and the percentage of excystation was compared with that from previous studies done on cysts obtained from the kidneys of snails. The percentage of excystation of E. triuoluis metacercariae from tadpole kidneys was similar to that reported for previous studies on cysts obtained from experimentally infected gastropod hosts. The possible role of tadpoles as an agent for the transmission of Echinostomn and echinostomiasis to humans is discussed.

• 한국환경보건학회지
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• 제5권1호
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• pp.66-70
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• 1978

This experiment is to search what some chemicals polluting water have an effect on the Rana nigromaculata Hallowell, in the survival rate and the metamorphosis speed from a tadpole to a young frog. 1. The fatal thickness by some agricultural chemicals and copper sulfate is different from one another. The fatal rate in the contrast group is 15 percent, and particulary Dithane M-45 is the most among the all reagents. 2. The effect on the anamorphosis is very severe. In the case of contrast group, it appears to stage 25, but it is not to appear at the group used agricultural chemicals. 3. The less the thickness of that is, the less the influence of the survival rate and the metamorphosis speed. 4. The density of the agricultural chemical is in inverse proportion to the survival rate and the metamorphosis speed, and the other side, the fatal rate is proportion.

• Animal Systematics, Evolution and Diversity
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• nspc3호
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• pp.59-66
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• 1992

경남 창녕 및 삼천포의 논에서 채집된 투구새우류 1종, Triops longicaudatus(LeConte,1846)을 재기재한다. 이것은 국내 최초의 배갑류에 대한 기록이다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.239-240
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• 2001

Collagenases are generally defined as enzymes capable of degrading the polypeptide backbone of native collagen under conditions which do not denature the protein. Two types of proteases with collagenolytic activity have been reported and thought to play different physiological functions. Metallo-collagenases, firstly discovered in tadpole tissue explants are zinc-containing enzymes requiring calcium for optimum activity and stability, and These enzymes have been widely studied from various mammalian tissues as well as from bacteria, such as Bacillus cereus, Clostridium histolyticum, Achromobacter, Vibrio alginolyticus and Clostridium perfringens and snake venoms. (omitted)

• Animal cells and systems
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• 제7권3호
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• pp.221-225
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• 2003

The tadpole larva of the ascidian Halocynthia roretzi has two sensory pigment cells in its brain vesicle. To elucidate the temporal requirement for FGF signaling in formation of the pigment cells, embryos were treated with an FGF receptor 1 inhibitor, SU5402, or an MEK inhibitor, U0126 during various embryonic stages. In the present study, it is shown that the embryos treated with SU5402 from the 16-cell stage to the early gastrula stage do not form pigment cells, whereas those treated after the early gastrula stage form pigment cells. In pigment cell formation, embryos suddenly exhibited the sensitivity to SU5402 only for 1 h at the neural plate stage(-4 h after the beginning of gastrulation). When U0126 treatment was carried out at various stages between the 8-cell and late neurula stages, the embryos scarcely formed pigment cells. Pigment cell formation occurred when the embryos were placed in U0126 at early tail bud stage. These results indicate that FGF signaling is involved in pigment cell formation at two separate processes during ascidian embryogenesis, whereas more prolonged period is required for MEK signaling.