• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.272-279
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• 2006

The gene encoding Thermus sp. T351 alkaline phosphatase (T351 APase) was cloned and sequenced. The gene consisted of 1,503 bp coding for a protein with 500 amino acid residues including a signal peptide. The deduced amino acid sequence of T351 APase showed relatively low similarity to other Thermus APases. The T351 APase gene was expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21 (DE3). The expressed enzyme was purified by heat treatment, and $UNO^{TM}$ Q and $HiTrap^{TM}$ Heparin HP column chromatographies. The purified enzyme exhibited high activity at extremely alkaline pHs, reaching a maximum at pH 12.0. The optimum temperature of the enzyme was $80^{\circ}C$, and the half-life at $85^{\circ}C$ was approximately 103 min. The enzyme activity was found to be dependent on metal ions: the addition of $Mg^{2+}$ and $CO^{2+}$ increased the activity, whereas EDTA inhibited it. With p-nitrophenyl phosphate as the substrate, T351 APase had a Michaelis constant ($K_{m}$) of $3.9{\times}10^{-5}M$. The enzyme catalyzed the hydrolysis of a wide variety of phosphorylated compounds.

• The Research Journal of the Costume Culture
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• v.20 no.1
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• pp.72-82
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• 2012

The purpose of this research to look into formative characteristics of eco T-shirt design. Previous study researchers have mostly focused on overall eco fashion design, but on the other hand the research on eco product entity leaves much to be desired. We analyzed formative characteristics of eco T-shirt design that is easily accessible in real life and is easy to passing message. We selected 23 eco brands through internet and analyzed total 500 photos of eco T-shirt. Each photo was categorized by sex and the nations which belong to the eco fashion world associations. The content and statistical analysis was used for data analysis. The content of the research is as the following. First, it was found out that basic straight silhouette, achromatic color, human pattern, plant pattern, and eco-friendly organic material were used for the design of eco T-shirt. Second, it showed difference of silhouette, color, pattern according to nations. Third, it showed difference of silhouette, color, pattern according to sex. Fourth, symbolism of eco T-shirt was nature love, nature support, society ethicality, anti-sociality, and economics. This research aims for providing practical help and assistance to the development of eco T-shirt and its relevant industries.

• The Korea Journal of Herbology
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• v.36 no.1
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• pp.67-76
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• 2021

Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development. Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 mg/kg) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment. Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured. Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines. Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.837-840
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• 2004

The purpose of this research was to investigate the effect of Eunkyo-San(EKS) on the immune system. Administration of EKS(500 mg/kg) enhanced viability of splenocytes and thymocytes in BALB/c mice, and also EKS increased of splenic B, T lymphocytes and thymic T lymphocytes, significantly increased CD4 positive TH cells. EKS markedly enhanced the production of -interferon in mice serum. EKS accelerated the production of nitric oxide in peritoneal macrophages. These results suggest that EKS have an immune-enhancing activity.

• Information and Communications Magazine
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• v.32 no.5
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• pp.23-30
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• 2015

인터넷 빅뱅은 세상의 모든 컴퓨터들을 연결하는 단계를 넘어 이제는 모든 사물들을 연결하는 사물인터넷(Internet of Things) 수준으로까지 확대되고 있다. 2020년이면 500억개 이상의 사물과 기기들이 연결되고, 이러한 사물들의 다양한 정보를 연동하고 제어함으로써 새로운 IT 시대가 열릴 것으로 전망되고 있다. 이러한 사물인터넷 확산에는 인터넷 기술뿐 아니라 고성능 디바이스 기술의 발달과 초고속 유무선 통신 기술, 클라우드/빅데이타와 같은 데이터 기술, 그리고 오픈소스와 같은 사용자 참여형 개방 기술의 발전도 중요한 밑거름이 되고 있다. 이에 본 고에서는"오픈소스 사물인터넷(OSIoT:Open Source Internet of Things)"동향과 전망을 살피고 거시적 관점에서의 사용자 참여와 개방 기술 중심의 사물인터넷의 발전 방향을 함께 살피고자 하였다.

• Journal of Environmental Science International
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• v.5 no.4
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• pp.513-524
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• 1996

Laboratory investigation was conducted to evaluat the mixing effects on organic removal efficiency to treat low-strength synthetic wastewater using modified anaerobic - filter reactor combining anaerobic filter and upflow anaerobic sludge blanket. Using the modified process the low-strength wastewater like municipal sewage could be treated with 85% T-COD removal efficiency at hydraulic retention time of 6 hours. At the constant organic loading of 0.5 kg COD/m 3-day, the organic removal efficiency and effluent COD concentration are increased as influent COD concentration increased from 125 mg/l to 500 mg/l. Mixing effects on organic removal efficiency are evident and optimum mixing speed is found as 50RPM. Placing the granular sludge and media on which slime layer was pre-formed into the reactor seemed to be very effective In achieving short start-up period. Therefore, the steady state was achived after 4 weeks and 1 week based on T-COD and S-COD, respectively.

• Journal of the Korean Operations Research and Management Science Society
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• v.22 no.3
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• pp.81-98
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• 1997

Given a rooted tree T with profits and node demands, the capacitated subtree of a tree problem (GSTP) consists of finding a rooted subtree of maximum profit, subject to having total demand no larger than the given capacity H. We first define the so-called critical item for CSTP and find an upper bound on the optimal value of CSTP in O(n$^{2}$) time, where n is the number of nodes in T. We then present our branch and bound algorithm for solving CSTP and illustrate the algiruthm by using an example. Finally, we implement our branch-and-bound algorithm and compare the computational results with those for both CPLEX and a dynamic programming algorithm. The comparison shows that our branch-and-bound algorithm performs much better than both CPLEX and the dynamic programming algorithm, where n and H are the range of [50, 500] and [5000, 10000], respectively.

• Proceedings of the KAIS Fall Conference
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• 2007.05a
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• pp.299-302
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• 2007

본 연구는 하 ${\cdot}$ 폐수 중의 유기물뿐만 아니라 질소, 인을 생물학적으로 제거하는 실험을 수행하였다. 공정 내 미생물의 유실을 방지하고 미생물이 고농도 상태로 유지 가능한 부착성장의 한 공법인 RBC에 끈상 나선형 미생물 접촉재를 설치한 반웅기를 이용하였다. 원수는 Glucose 1,800 mg/L, $NH_{4}Cl$ 500 mg/L, $KH_{2}PO_{4}$ 5mg/L를 혼합한 인공폐수를 제조하여 공정에 주입하였고, 그 결과 각각 미생물이 폐수에 적응하는 단계인 Period 1에서는 각 수질 분석 항목의 농도가 점차적으로 감소하는 경향을 보였으며, 정상상태라고 판단한 Period 2에서는 최종적으로 유입수에 대한 유출수의 제거율은 각각 $TCOD_{Cr}$ 94%, BOD 87%, T-N 85%, T-P 89%의 결과를 나타내었다.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05c
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• pp.341-346
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• 1996

사용후핵연료에 대한 장기건식저장과 관련하여 원자로에서 조사된 사용후 핵연료피복관에 대한 산화시험을 공기분위기에서 수행하였다. 피복관 시료의 50$0^{\circ}C$ 공기중 산화시험 결과 산화 초기에 급격한 산화율을 보였으며, 이 후 천이점까지 느리게 산화가 진행되다가 천이 후에는 선형적으로 급격히 무게가 증가하는 지르코늄 합금의 수증기 및 공기중에서의 전형적인 산화양상을 나타내었다. 시편별로는 가장 두꺼운 노내 산화막을 가진 시편이 가장 높은 산화율을 나타내었으며, 노내 산화시 천이점에 근접한 시편들이 가장 낮은 산화율을 보였다. 산화율이 가장 높은 시편의 천이후 영역에서의 산화율은 $\Delta$W ＝ 0.74 t ＋ 38.61과 같은 관계식으로 표현될 수 있었다. 이 때 $\Delta$W는 무게이득(mg/dm$^2$)이고 t는 산화시간(h)을 나타낸다. 시험에 사용된 피복관의 단위 산화막두께(l$\mu$m)에 대한 산화무게증가량은 약 13.4mg/dm$^2$으로 나타났다. 이러한 결과들은 사용후핵연료 중간저장 시설 및 저장캐스크의 설계 전산코드 작성 및 저장시설의 운영에 관련되어 기반자료로 활용될 수 있을 것이다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2001.11a
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• pp.26-27
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• 2001

SNCR 기술을 이용한 NOx 저감은 반응온도 850～$1100^{\circ}C$에서 효과적이였으며, 몰비($NH_3$/NOx), $O_2$ 농도에 상당히 영향을 받고 있었다. 최적온도 $950^{\circ}C$, 몰비 1.5에서 약65%의 NOx 제거효율을 얻을 수 있으며, 온도구배가 없고 $O_2$ 농도가 2～4%로 낮아질 경우 더 높은 제거효율이 기대된다. SCR 기술에 의한 NOx 저감을 위해 $V_3$$O_{5}$/-$WO_3$/$TiO_2$ 상용촉매를 사용하였으며, 반응 온도는 200～$500^{\circ}C$ 범위로 확인되었으며, 약 84%의 NOx 제거효율이 몰비 1.5 에서 얻어졌다. $O_2$ 농도가 21%로 높아짐에 따라 상당히 효율이 떨어짐이 밝혀졌다. SNCR/SCR combined 시스템은 몰비=2.0, $T_{SNCR}$/=$850^{\circ}C$, $T_{SCR}$ /=$350^{\circ}C$ 반응조건에서는 약 93%의 NOx 저감효율을 보여주어 SNCR, SCR 단위기술보다 더 효과적이었다.