• Title/Summary/Keyword: T500

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Isolation and Characterization of Immunomodulatory Glycoprotein from the Root of Panax ginseng

  • Shin, Han-Jae;Park, Kyeong-Mee;Kim, Young-Sook;Nam, Ki-Yeul;Lee, You-Hui;Park, Jong-Dae
    • Journal of Ginseng Research
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    • v.24 no.3
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    • pp.128-133
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    • 2000
  • A high molecular (more than 10 kDa) fraction, showing mitogenic and comitogenic activities in spleen cells of mouse, was isolated from water extract of ginseng. The crude protein substance prepared by 80% (NH$_4$)$_2$SO$_4$ precipitation from this fraction was purified and isolated by DEAE Sepharose column chromatography. Among the fractions eluted, it was found that four kinds of fractions eluted with 0 to 1 M NaCl gradient were glycoproteins, which induced proliferation of spleen cells and increased NO production in macrophages. Among them, F-2 fraction, which contained 35.9% protein,49.4% neutral sugar and 12.5% uronic acid, was found to show mitogenic activity as strong as that of LPS (lipopolysaccharide) at a concentration of 100 $\mu\textrm{g}$/ml and to remarkably stimulate NO production by murine macrophages at a concentration of 500 $\mu\textrm{g}$/ml. When F-2 is deproteinized, the mitogenic activity of F-2 was decreased significantly to 70.9% as compared with that of F-2. This results suggests that the protein moiety of F-2 may play an important role in immunomodulating activity of glycoprotein from the root of Panax ginseng.

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A Study on Education Need and Effective Network Formation for the KNOU Nursing Students (방송대 간호학생의 교육요구 및 효율적 네트워크 구성에 관한 조사연구)

  • Lee Sang-Mi;Kim Young-Im;Lee Sun-Ock;Geon Hyo-Geon
    • The Journal of Korean Academic Society of Nursing Education
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    • v.4 no.2
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    • pp.236-248
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    • 1998
  • This survey study was attempted for two purposes : 1) to grasp Korea National Open University(KNOU) students' changing aspects for their education need through comparison analyses with 1996 data ; 2) to establish foundation of the systematic network formation by investgating students' opinion about network framework. Among randomly assigned 4,500 students, 1,505 KNOU nursing students who allowed to participate in the study completed the questionnaires. The data were collected by mail. For the comparison 1996 data were also used. The data were analyzed using descriptive statistics, chi-square test, and t-test. Results of this study were as follows 1. The admission purposes of the KNOU nursing students were 'in order to get a bachelor's degree (70.7%)', 'to do studying and working simultaneously(43.0%)', or 'to be admitted for the graduate school (41%)' etc. Comparing the admission purposes by age, the investigator found 4 items which are 'small amount of tuition', 'graduate school admission', 'aspiration for the university', 'promotion or commencement of work' showed statistically significant differences. These 4 items were also found to show significant differences by marital status. 2. In relation to the learning media, the study showed most students(74%) got effective informations from the school newspaper(36.5%) or peer group(37.7%). The result showed that few students (0.7%) used the computer for communication. The research indicated that KNOU nursing students have tendency to rely on printed materials more than on broadcasting media. This is almost the same result as that of 1996. 3. The results revealed that 12.4% of the respondents had ever experienced unregistration or temporary withdrawal. The most common reason for the unregistration was 'due to family affairs or their job (71.3%)'. There were no change for this aspects with 1996. 4. As for the professors-students network formation. The result revealed that 38.5% students among respondents had heard of the network formation. 78.7% of respondents, however, positively responed that they would willingly participate in the networking if it is made. Especially the students showed much interest in 'the improvement for the understanding of study' and 'strengthening of the relations between professors and students'.

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The Automatic Extraction of Hypernyms and the Development of WordNet Prototype for Korean Nouns using Korean MRD (Machine Readable Dictionary) (국어사전을 이용한 한국어 명사에 대한 상위어 자동 추출 및 WordNet의 프로토타입 개발)

  • Kim, Min-Soo;Kim, Tae-Yeon;Noh, Bong-Nam
    • The Transactions of the Korea Information Processing Society
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    • v.2 no.6
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    • pp.847-856
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    • 1995
  • When a human recognizes nouns in a sentence, s/he associates them with the hyper concepts of onus. For computer to simulate the human's word recognition, it should build the knowledge base (WordNet)for the hyper concepts of words. Until now, works for the WordNet haven't been performed in Korea, because they need lots of human efforts and time. But, as the power of computer is radically improved and common MRD becomes available, it is more feasible to automatically construct the WordNet. This paper proposes the method that automatically builds the WordNet of Korean nouns by using the descripti on of onus in Korean MRD, and it proposes the rules for extracting the hyper concepts (hypernyms)by analyzing structrual characteristics of Korean. The rules effect such characteristics as a headword lies on the rear part of sentences and the descriptive sentences of nouns have special structure. In addition, the WordNet prototype of Korean Nouns is developed, which is made by combining the hypernyms produced by the rules mentioned above. It extracts the hypernyms of about 2,500 sample words, and the result shows that about 92per cents of hypernyms are correct.

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Electrical Property of the Li2O-2SiO2 Glass Sintered by Spark Plasma Sintering (Spark Plasma Sintering으로 제조한 Li2O-2SiO2 유리 소결체의 전기적 특성)

  • Yoon, Hae-Won;Song, Chul-Ho;Yang, Yong-Seok;Yoon, Su-Jong
    • Korean Journal of Materials Research
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    • v.22 no.2
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    • pp.61-65
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    • 2012
  • A $Li_2O-2SiO_2$ ($LS_2$) glass was investigated as a lithium-ion conducting oxide glass, which is applicable to a fast ionic conductor even at low temperature due to its high mechanical strength and chemical stability. The $Li_2O-2SiO_2$ glass is likely to be broken into small pieces when quenched; thus, it is difficult to fabricate a specifically sized sample. The production of properly sized glass samples is necessary for device applications. In this study, we applied spark plasma sintering (SPS) to fabricate $LS_2$ glass samples which have a particular size as well as high transparency. The sintered samples, $15mm\phi{\times}2mmT$ in size, ($LS_2$-s) were produced by SPS between $480^{\circ}C$ and $500^{\circ}C$ at 45MPa for 3~5mim, after which the thermal and dielectric properties of the $LS_2$-s samples were compared with those of quenched glass ($LS_2$-q) samples. Thermal behavior, crystalline structure, and electrical conductivity of both samples were analyzed by differential scanning calorimetry (DSC), X-ray diffraction (XRD) and an impedance/gain-phase analyzer, respectively. The results showed that the $LS_2$-s had an amorphous structure, like the $LS_2$-q sample, and that both samples took on the lithium disilicate structure after the heat treatment at $800^{\circ}C$. We observed similar dielectric peaks in both of the samples between room temperature and $700^{\circ}C$. The DC activation energies of the $LS_2$-q and $LS_2$-s samples were $0.48{\pm}0.05eV$ and $0.66{\pm}0.04eV$, while the AC activation energies were $0.48{\pm}0.05eV$ and $0.68{\pm}0.04eV$, respectively.

Kinetic Investigation of CO2-CH4 Reaction over Ni/La2O3 Catalyst using Photoacoustic Spectroscopy

  • Oh, Hyun-Jin;Kang, Jin-Gyu;Heo, Eil;Lee, Sung-Han;Choi, Joong-Gill
    • Bulletin of the Korean Chemical Society
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    • v.35 no.9
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    • pp.2615-2620
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    • 2014
  • Ni/$La_2O_3$ with a high dispersion was prepared by reduction of $La_2O_3$ perovskite oxide to examine the catalytic activity for the $CO_2-CH_4$ reaction. The Ni/$La_2O_3$ catalyst was found to be highly active for the reaction. The ratios of $H_2$/CO were measured in a flow of the reaction mixture containing $CO_2/CH_4$/Ar using an on-line gas chromatography system operated at 1 atm and found to be varied with temperature between 0.66 and 1 in the temperature range of $500-800^{\circ}C$. A kinetic study of the catalytic reaction was performed in a static reactor at 40 Torr total pressure of $CO_2/CH_4/N_2$ by using a photoacoustic spectroscopy technique. The $CO_2$ photoacoustic signal varying with the concentration of $CO_2$ during the catalytic reaction was recorded as a function of time. Rates of $CO_2$ disappearance in the temperature range of $550-700^{\circ}C$ were obtained from the changes in the $CO_2$ photoacoustic signal at early reaction stage. The plot of ln rate vs. 1/T showed linear lines below and above $610^{\circ}C$. Apparent activation energies were determined to be 10.4 kcal/mol in the temperature range of $550-610^{\circ}C$ and 14.6 kcal/mol in the temperature range of $610-700^{\circ}C$. From the initial rates measured at $640^{\circ}C$ under various partial pressures of $CO_2$ and $CH_4$, the reaction orders were determined to be 0.43 with respect to $CO_2$ and 0.33 with respect to $CH_4$. The kinetic results were compared with those reported previously and used to infer a reaction mechanism for the Ni/$La_2O_3$-catalyzed $CO_2-CH_4$ reaction.

The opening efficiency of the existent net of the anchovy boat seine (기선권현망 현용 어구의 전개 성능)

  • AN, Young-Su;BACK, Young-Su;JIN, Song-Han;JANG, Choong-Sik;KANG, Myoung-Hee;CHA, Bong-Jin;KIM, Bo-Yeon;CHA, Ju-Hyeng;CHO, Youn-Hyoung
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.54 no.1
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    • pp.1-11
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    • 2018
  • This study was conducted in order to improve fishing gear for existent net of the anchovy boat seine. Field experiments were performed to observe geometry of nets by catcher boats. When the distances between the two ships were 100, 300, and 500 m, and the speeds of towing nets were 0.6, 0.9, and 1.2 k't, respectively. The vertical opening and actual opening of each part of the existent net was as follows: the middle part of the wing net, 12.9-26.6 m, 19-39%; the entrance of the inside wing net, 23.3-35.3 m, 40-60%; the square and bosom, 18.4-24.2 m, 37-49%; the entrance of the bag net, 19.0-23.3 m, 79-96%; the flapper, 13.2-15.3 m, 142-161%; and the end of the bag net, 13.2-15.7 m, 51-61%. The actual net opening of each part of the existent nets used in this study was lower than that of the design net height, due to the low net height of the wing net and the inside wing net, it limited a range of the net height of the square and bosom. The opening of the entrance of the bag net caused the net pocket shape and inflated some parts of the nets. The tension of towing nets was measured between 4.4 and 11.0 tons, and it is necessary to reduce the structure and improve the structure of the bag net.

An Effect of Sex education for Knwledge and Attitude toward Sex in Elementary School Students (성교육이 초등학생의 성에 대한 지식과 태도에 미치는효과)

  • Kim Yeong-Hee;Lee Myung-Hwa
    • The Journal of Korean Academic Society of Nursing Education
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    • v.3 no.2
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    • pp.255-275
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    • 1997
  • This study was conducted to find out the effect of sexual education for knowledge and attitude toward sex are elementary school students. Therefore, the present study aims to understand the changes of the knowledge are the attitude toward sex before and after the sex education, in elementary school students and then finally to provide some basic data to suggest directions for sex education. The subjects were 500 male and female elementary students from the fifth and sixth grades of 4 public elementary schools in Pusan. The subjects were assigned to a experimental (250) and a control (250) group. The research tools were sex education program and consists of physiology(menache, ejaculation), psycological(pleasure of growth), sociological(hetero sexual relationship) are about set The sex education program was given to experimental group for 6 hours by the trained school nurse's lecture using slide film and OHP film. The data were obtained before and after the sex education. Data collection was done during the period betwen Feb. 3. 1997. to Feb. 20. 1997. The collected data were analyed using frequency, percentages, $X^2$-test, means, standard deviation, t-test, ANOVA, MANCOVA with SPSS program. The results were obtained as follows : 1. The first hypotheses attained that the experimental group(19.72) is higher than the control group(12.12) on the knowledge toward sex(F=467.30, P=.000). At the physical area, the experimental group(8.84) is higher than the control group(3.96) (F=687.39, P=.000). At the psychological area the experimental group (5.18) is higher than the control group(2.63) (F=411.66, P=.000). At the social area, the experimental group (5.70) is higher than the control group (5.54), this is not significant difference (F=.67, P=.413). 2. The second hypotheses attained that the experimental group(60.42) is higher than the control group(52.48) on the attitude toward sex(F=215.70, P=.000). At the physical area, the experimental group(23.10) is higher than the control group(20.68) (F=128.68, P=.000), At the psychological area, the experimental group(20.11) is higher than the control group(16.40) (F=108.64, P=.000). At the social area, the experimental group(17.22) is higher than the control group(16.00), this is area are significant differences (F=70.37, P=.000). 3. Knowledge toward sex according to sex, religon, atmosphere of family, mother age and educational background are significant differance (P<0.05). Attitude toward sex according to sex is significant differance (P<0.05).

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Effects of $1{\alpha}$-Hydroxycholecalciferol and Phytase on Growth Performance, Tibia Parameter and Meat Quality of 1- to 21-d-old Broilers

  • Han, J.C.;Yang, X.D.;Zhang, L.M.;Li, W.L.;Zhang, T.;Zhang, Z.Y.;Yao, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.6
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    • pp.857-864
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    • 2009
  • This experiment was conducted to investigate the effects of interaction between $1{\alpha}$-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) and phytase on growth performance, parameters of tibia and plasma, and meat quality of 1- to 21-d-old broilers. Two hundred and forty male, 1-d-old Arbor Acres broilers were randomly assigned to 20 cages, with 12 chicks per cage. Five treatments were designed, with four cages each. A 2${\times}$2 factorial experiment was designed to test 0 and 5 ${\mu}g/kg$ of $1{\alpha}$-OH $D_3$ in combination with 0 and 500 U/kg of phytase. A basal diet was formulated to contain 2.9 g/kg of non-phytate phosphorus (NPP), and the control diet was formulated to contain a normal level of NPP (4.5 g/kg). Results showed that $1{\alpha}$-OH $D_3$ alone increased tibia ash, contents of calcium and phosphate, breaking strength, concentrations of plasma calcium and phosphate, and water-holding capacity of breast and thigh meat, while it decreased growth of broilers. Phytase alone improved performance and tibia quality. Although growth of broilers was lower than that of the positive control when the diet was supplemented with $1{\alpha}$-OH $D_3$ and phytase, tibia quality was significantly improved by the addition of $1{\alpha}$-OH $D_3$ and phytase. These data suggest that interaction between $1{\alpha}$-OH $D_3$ and phytase at 2.9 g/kg of dietary NPP could significantly increase bone quality of 1- to 21-d-old broilers, while not improving growth performance.

Effect of Banded Subsoil Fertilization on the Yields and N Utilization of Radish (Raphanus sativus L.) in Plastic Film Mulching Cultivation (무 재배시 질소이용률 및 수량에 미치는 토중시비 효과)

  • Yang, Chang-Hyu;Yoo, Chul-Hyun;Shin, Bok-Woo;Kang, Seung-Won
    • Korean Journal of Environmental Agriculture
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    • v.24 no.4
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    • pp.398-403
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    • 2005
  • To establish law-put fertilization technique and increase of fertilization efficiency during cultivation of plastic film mulching for plastic the improvement of soil properties, nutrition efficiency and yield by banded subsoil fertilization (BSF) using band spoty applicator was conducted at radish (Raphanus sativus L.) field in Honan Agricultural Research Institute from 1997 to 1998 for 2 years. These results were as follows. T-N, available $P_2O_5$ exchangeable Ca and K contents of soil were increased those of before experiment especially in BSF treatment. Also, the content of soil $NO_3-N$ was higher in BSF as fertilization amount is increasing than in CF (conventional fertilization). Uptake amounts of nitrogen fertilized were high in BSF during overall the growth period of plant and N utilization was high by $21.9{\sim}30.4%$ in BSF compared to in CF. The rate of fertilizer loss by rainfall was largely reduced, because all fertilizer applied was putted around the root zone. The total yields of fresh radish BSF treatments were more increased $13{\sim}37%$ than that of CF treatment.

Screening of Cytotoxicity and Antimicrobial Effects of Extracts from Atractylodes macrocephala Koidz (백출 추출물의 세포독성과 항균효과검색)

  • Choi Eun Young;Oh Hyun Ju;Park Nang Kyu;Chun Hyun Ja;Ahn Jong Woog;Jeon Byung Hun;Han Du Seok;Lee Hyun Ok;Baek Seung Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.2
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    • pp.348-352
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    • 2002
  • This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents