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Evaluation of Magnetization Transfer Ratio Imaging by Phase Sensitive Method in Knee Joint (슬관절 부위에서 자화전이 위상감도법에 의한 자화전이율 영상 평가)

  • Yoon, Moon-Hyun;Seung, Mi-Sook;Choe, Bo-Young
    • Progress in Medical Physics
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    • v.19 no.4
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    • pp.269-275
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    • 2008
  • Although MR imaging is generally applicable to depict knee joint deterioration it, is sometimes occurred to mis-read and mis-diagnose the common knee joint diseases. In this study, we employed magnetization transfer ratio (MTR) method to improve the diagnosis of the various knee joint diseases. Spin-echo (SE) T2-weighted images (TR/TE 3,400-3,500/90-100 ms) were obtained in seven cases of knee joint deterioration, FSE T2-weighted images (TR/TE 4,500-5,000/100-108 ms) were obtained in seven cases of knee joint deterioration, gradient-echo (GRE) T2-weighted images (TR/TE 9/4.56/$50^{\circ}$ flip angle, NEX 1) were obtained in 3 cases of knee joint deterioration, In six cases of knee joint deterioration, fat suppression was performed using a T2-weighted short T1/tau inverse recovery (STIR) sequence (TR/TE =2,894-3,215 ms/70 ms, NEX 3, ETL 9). Calculation of MTR for individual pixels was performed on registration of unsaturated and saturated images. After processing to make MTR images, the images were displayed in gray color. For improving diagnosis, three-dimensional isotropic volume images, the MR tristimulus color mapping and the MTR map was employed. MTR images showed diagnostic images quality to assess the patients' pathologies. The intensity difference between MTR images and conventional MRI was seen on the color bar. The profile graph on MTR imaging effect showed a quantitative measure of the relative decrease in signal intensity due to the MT pulse. To diagnose the pathologies of the knee joint, the profile graph data was shown on the image as a small cross. The present study indicated that MTR images in the knee joint were feasible. Investigation of physical change on MTR imaging enables to provide us more insight in the physical and technical basis of MTR imaging. MTR images could be useful for rapid assessment of diseases that we examine unambiguous contrast in MT images of knee disorder patients.

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Suppressive Effect of Acorn (Quercus acutissima Carr.) Extracts in 3T3-L1 Preadipocytes (도토리 추출물의 3T3-L1 세포 분화억제 효과)

  • Kim, Ji-Yeon;Lee, Jin;Lee, Chang-Won;Kim, Ae-Jung
    • The Korean Journal of Food And Nutrition
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    • v.28 no.4
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    • pp.650-657
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    • 2015
  • This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

The Application of Computer Program for Determination of Fluid Properties and P-T Condition from Microthermometric Data on Fluid Inclusions (유체포유물의 생성시 온도-압력 조건과 유체포유물의 물리화학적 특성연구에 있어서의 컴퓨터 프로그램이용)

  • Oh, Chang-Whan;Choi, Sang-Hoon
    • Economic and Environmental Geology
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    • v.26 no.1
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    • pp.21-27
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    • 1993
  • Fluid inclusion has been widely used to study the origin and physiochemical conditions of ore deposits. However, it is difficult to get the accurate physiochemical data from fluid inclusion study due to the error of microthermometric data and the complexity of calculation of density and isochore of fluid inclusion. The computer programs HALWAT, $CO_2$, and CHNACL written by Nicholls and Crowford (1985) partly contributed to improve the accuracy of physiochemical data by using complicated equations. These programs are applied to determine the densities and isochores of fluid inclusions for the Cretaceous Keumhak mine using Choi and So's data (1992) and for the Jurassic Samhwanghak mine using Yun's data (1990). The estimated PoT for Keumhak mine from calculated isochores of coexisting fluid inclusions are $230^{\circ}{\sim}290^{\circ}C$ and 500~800 bar which matche well to the poT estimated by Choi and So ($280^{\circ}{\sim}360^{\circ}C$ and 500~800 bar, 1992). However, the poT for Samwhanghak mine estimated in this study by combining the calculated isochores and sulfur isotope geothermometer data by Yun (1990) are about 4~7 kb at $329{\pm}50^{\circ}{\sim}344{\pm}55^{\circ}C$ which are quite different form the P-T estimates by Yun ($255^{\circ}{\sim}294^{\circ}C$ and 1.2~1.9kb, 1990). This discrepancy caused by misinterpretation of homogenization temperature (Th) of fluid inclusion and by application of inappropriate isochores. The application of homogenization temperature and/or inappropriately selected isochore to determine the trapping PoT condition of ore-deposits should be avoided, particularly for ore-deposits formed at pressures higher than 1~2 kb.

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Characterization of a Bacteriocin Produced by Enterococcus sp. T7 Isolated from Humans

  • Moon, Hi-Seong;Jeong, Jong-Jin;Ji, Geun-Eog;Kim, Jong-Sang;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.507-513
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    • 2000
  • A bacteriocin-producing organism, Enterococcus sp. T7, was isolated from human fecal samples. Bacteriocin T7, named tentatively as the bacteriocin, was produced by Enterococcus sp. T7 and it inhibited some strains of Lactobacillus. Staphylococcus, Enterococcus, and Streptococcus, but not all the lactococci and gram-negative bacteria tested. Bacteriocin T7 inhibited the growth of Listeria monocytogenes Scott A, but the degree of inhibition was less than those for other sensitive gram-positive vacteria. Bacteriocin T7 in MRS broth started to produce at the middle of the exponential growth phase and the inhibitory activity reached its maximum level during the stationary growth phase. Bacteriocin T7 was stable against heat treatments, pH variations (pH 2-10), and exposure to organic solvents. The molecular weight of bacteriocin T7 was estimated to be 6.500 Da by SDS-PAGe. All these facts, including physico-chemical stabilities, small molecular size, and inhibition of Kisteria monocytogenes, indicate that bacteriocin T7 is likely to be a member of the class IIa bacteriocins.

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Study on Creep Life Prediction by Initial Strain Method for Friction Welded Joints of Heat Resisting Steels (내열강 마찰용접재의 ISM에 의한 크리프 수명예측에 관한 연구)

  • 김헌경;김일석;이연탁;공유식;오세규
    • Journal of Ocean Engineering and Technology
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    • v.15 no.2
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    • pp.46-52
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    • 2001
  • In this paper, the real-time prediction of high temperature creep life was carried out for the friction welded joints of dissimilar heat resisting steels (SUH3-SUH35). various life prediction method such as LMP (Larson_miller Parameter) and ISM (initial strain method) were applied. The creep behaviors of those steels and the welds under static load were examined by ISM combined with LMP at 500, 600 and $700^{\circ}C$, and the relationship between these two methods was investigated. A real-time creep lie (tr, hr) prediction equation by initial strain (${\varepsilon}_0$, %) under any creep stress ($\sigma$, MPa) at any high temperature (T, K) was developed as follows: $t_r={\alpha}{\varepsilon}_0^{\beta}{\sigma}^{-1}$ where, ${\phi}=16: {\alpha}=10^{51.412-0.104T+5.375{\times}10^5T^2}$, $ {\beta}=-83.989+0.180T-9.957{\times}10^{-5}T^2,{\phi}=20:$ ${\alpha}=10^{69.910-0.146T+7.744{\times}10^{-5}T^2$, ${\beta}=-51.442+0.105T-5.595{\times}10^{-5}T^2$ for SUH3-SUH35 friction weld of =16mm and 20mm, respectively.

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Effects of Blended Essential Oil(CRINA®) Supplementation on the Performance, Nutrient Digestibility, Small Intestinal Microflora and Fatty Acid Composition of Meat in Broiler Chickens (사료중 Blended Essential Oil(CRINA®) 첨가가 육계의 생산성과 영양소 이용률, 소장 내 미생물 균총 및 계육내 지방산 조성에 미치는 영향)

  • Suk, J.C.;Lim , H.S.;Paik, I.K.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.777-786
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    • 2003
  • An experiment was conducted to investigate the effects of supplementary Blended essential oil(CRINA$^{\circledR}$) on the performance, nutrient availability, fatty acid composition of leg muscle, small intestinal microflora and blood parameters in broiler chickens. One thousand unsexed day-old broiler chickens were assigned to five treatments : control(T1), 5ppm avilamycin(starter diet) & 5ppm flavomycin(grower diet) T2, 5ppm avilamycin(starter diet) & 50ppm CRINA$^{\circledR}$(grower diet) T3, 50ppm CRINA$^{\circledR}$(starter & grower diet) T4, 50ppm CRINA$^{\circledR}$+ 500ppm lactic acid$^{\circledR}$ (starter & grower diet) T5. Each treatment had four replications of 50 birds each. Growth performance was significantly improved by dietary supplements(T2-T5). There were no significant differences among treatment T2, T3, T4 and T5. Feed intake was not significantly different among treatments. Dietary supplementation of CRINA$^{\circledR}$(T3, T4, T5) resulted in significant(p〈0.05) improvement in feed/gain(F/G) during finishing period (4-5weeks). The birds fed CRINA$^{\circledR}$ supplemented diet(T4) showed significantly(p〈0.05) higher availability of crude fat, methionine and methionine + cystine than those fed antibiotics supplemented diet(T2). Mortality was not significantly affected by treatments. The colony forming unit(CFU) of E.coli in small intestinal content was significantly lower in antibiotics & CRINA$^{\circledR}$(T3) compared to CRINA$^{\circledR}$ treatment(T4)(P〈0.05). CFU of Cl. perfringens was low in CRINA$^{\circledR}$(T4) but not different significantly with other treatments. Serum triglyceride level of birds fed CRINA$^{\circledR}$ + lactic acid diet(T5) was significantly lower(p〈0.05) than those fed antibiotics supplemented diet(T2). Cholesterol level of the birds fed antibiotics(T2) or CRINA$^{\circledR}$ + lactic acid supplemented diet(T5) was significantly higher(p〈0.05) than other treatments. HDL level of birds fed control diet was significantly lower(p〈0.05) than that of others. The levels of serum IgG were not significantly different among treatments. Major fatty acids composition of leg muscle fat was significantly influenced by treatments. Control group showed significantly higher palmitic acid(C$_{16:0}$) and steraric acid(C$_{18:0}$) content than other treatments(p〈0.05). Content of oleic acid(C$_{18:1}$), however, was significantly lower in the control than others treatments. Content of linolenic acid(C$_{18:3}$) was significantly higher in CRINA$^{\circledR}$+ lactic acid(T5) than antibiotics & CRINA$^{\circledR}$(T3) treatments. Total saturated fatty acids content was higher and total unsaturated fatty acids were lower in the leg muscle fat of the control than that of other treatments. It is concluded that CRINA$^{\circledR}$ supplementation improved growth rate and F/G ratio in broilers. The combination of CRINA$^{\circledR}$ with either antibiotics or lactic acid did not show any additive or synergistic effects in broiler chickens .

Fruit Quality and Harvest Time of 'Heukboseok' Grape by Fruit Load (착과량에 따른 '흑보석' 포도의 과실 품질 및 수확기)

  • Jung, MyungHee;Kwon, YongHee;Lee, ByulHaNa;Park, YoSup;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.289-295
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    • 2014
  • This research was conducted to investigate a difference in quality according to control of fruit load and cluster weight when seeded 'Heukboseok' grape was grown in a conventional cultivation system. Clusters per vine were set to harvest 1,500 kg, 1,800 kg, and 2,200 kg grapes per $990m^2$. In p lots t reated t o produce 1,800 kg grapes, clusters were set to 3 grades produce grapes with 350 g, 500 g, and 700 g in cluster weight. Based on color chart (National Institute of Horticultural & Herbal Science, Rural Development Admnistration) which show maturation stage for 'Kyoho' grape, grapes with higher than grade 9 were harvested at 80 and 90 days after full bloom, and the r est of them were harvested a t 100 days after full bloom. The final yield of 'Heukboseok' grape was the lowest in target yield of 1,800 kg with 700 g clusters with 75.5% of target yield, but 92.1%-100.1% of target yield were harvested in other treatments. Among treatments with several fruit setting, 83.3% of grapes harvested in treatment to produce 1,500 kg grapes as a target yield at 90 days after full bloom. Among the treatments with different cluster weights, 93.5% grapes were harvested in the treatment with 350 g in cluster weight. 'Heukboseok' grape showed rapid maturation pattern with no significant difference in either soluble solids content or acidity between 90 days from 80 days after full bloom. Therefore, to prevent flesh softening, it is appropriate to harvest fruit at 90 days after full blooms, and both yield and cluster weight were important factors influencing the quality of the fruits in 'Heukboseok' grape. It is confirmed that coloring and firmness was influenced by the final yield and weight per cluster, respectively. Therefore, the yield should be set as grape of 1,500 kg per $990m^2$ in target yield, and cluster weight should be adjusted to 350 g to produce grape of 1,800 kg with the fruits of high quality. It is considered to be hard to produce grape of high quality with 700 g in cluster weight in 'Heukboseok' grape.

Effects of MLSS Concentration and Influent C/N Ratio on the Nitrogen Removal Efficiency of Alternately Intermittently Aerated Nonwoven Fabric Filter Bioreactors (교차 간헐 포기식 부직포 여과막 생물반응조에서 MLSS 농도 및 유입수 C/N 비가 질소 제거효율에 미치는 영향)

  • Jung, Kyoung-Eun;Bae, Min-Su;Lee, Jong-Ho;Cho, Yun-Kyung;Cho, Kwang-Myeung
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.5
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    • pp.501-510
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    • 2006
  • To investigate the effects of MLSS concentration and influent C/N ratio on the nitrogen removal efficiency of alternately intermittently aerated nonwoven fabric filter bioreactors, the MLSS concentrations of the reactors were maintained at approximately 5,500 mg/L, 10,000 mg/L and 15,000 mg/L, and the influent TCOD/TKN ratio was decreased gradually from 5 to 2 by adding $NH_4Cl$. The influent was prepared by diluting a food waste leachate to a TCOD concentration of about 300 mg/L. The results of the experiment showed F/M ratios less than 0.112 g TCOD/g MLSS-day, average TCOD removal efficiencies of above 95%, and an average observed microbial yield coefficient of 0.283 g MLSS/g COD removed. The nitrification efficiencies were computed to be always better than 96% except one case where the nitrification efficiency was 90.5% when the MLSS concentration and the influent TCOD/TKN ratio was 5,500 mg/L and 2, respectively. The denitrification efficiency deteriorated as the influent TCOD/TKN ratio decreased. The average denitrification efficiency at the MLSS concentration of 10,000 mg/L was 10.7% better than that at the MLSS concentration of 5,500 mg/L, and the denitrification rate improved at a rate of 2.66 mg NL as the MLSS concentration increased by 1,000 mg/L. When the MLSS concentration was 15,000 mg/L, however, the average denitrification efficiency was merely 4.6% higher compared to when the MLSS concentration was 5,500 mg/L, and the denitrification rate increased at a rate of 0.75 mg N/L per 1,000 mg/L MLSS increase. Therefore, no strict proportional relationship was found between MLSS concentration and endogenous denitrification rate. The average alkalinity consumption rate was 3.36 mg alkalinity/mg T-N removed, which is similar to the theoretical value of 3.57 mg alkalinity/mg T-N removed, but the rate increased as the influent TCOD/TKN ratio decreased.